Transgenic mice expressing a soluble form of porcine nectin-1/herpesvirus entry mediator C as a model for pseudorabies-resistant livestock
An approach to genetically engineered resistance to pseudorabies virus (PRV) infection was examined by using a transgene encoding a soluble form of nectin-1, also known as herpesvirus entry mediator C. Nectin-1 is an alpha-herpesvirus receptor that binds to virion glycoprotein D. Nectin-1 mediates e...
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| Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 2004-11, Vol.101 (46), p.16150-16155 |
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| container_title | Proceedings of the National Academy of Sciences - PNAS |
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| creator | Ono, E Amagai, K Taharaguchi, S Tomioka, Y Yoshino, S Watanabe, Y Cherel, P Houdebine, L.M Adam, M Eloit, M |
| description | An approach to genetically engineered resistance to pseudorabies virus (PRV) infection was examined by using a transgene encoding a soluble form of nectin-1, also known as herpesvirus entry mediator C. Nectin-1 is an alpha-herpesvirus receptor that binds to virion glycoprotein D. Nectin-1 mediates entry of PRV, herpes simplex virus types 1 and 2, and bovine herpesvirus type 1. To assess the antiviral potential of an ectopic expression of the nectin-1 ectodomain in vivo, six transgenic mouse lines expressing a soluble form of nectin-1, consisting of an extracellular domain of porcine nectin-1 and the Fc portion of human IgG1, were generated. All of the transgenic mouse lines showed nearly complete resistance to PRV infection by means of both i.p. and intranasal routes. These results suggest that the introduction into farm animals of a transgene encoding a soluble form of nectin-1 would offer a potent biological approach to generating alpha-herpesvirus-resistant livestock. |
| doi_str_mv | 10.1073/pnas.0405816101 |
| format | Article |
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Nectin-1 is an alpha-herpesvirus receptor that binds to virion glycoprotein D. Nectin-1 mediates entry of PRV, herpes simplex virus types 1 and 2, and bovine herpesvirus type 1. To assess the antiviral potential of an ectopic expression of the nectin-1 ectodomain in vivo, six transgenic mouse lines expressing a soluble form of nectin-1, consisting of an extracellular domain of porcine nectin-1 and the Fc portion of human IgG1, were generated. All of the transgenic mouse lines showed nearly complete resistance to PRV infection by means of both i.p. and intranasal routes. These results suggest that the introduction into farm animals of a transgene encoding a soluble form of nectin-1 would offer a potent biological approach to generating alpha-herpesvirus-resistant livestock.</description><identifier>ISSN: 0027-8424</identifier><identifier>EISSN: 1091-6490</identifier><identifier>DOI: 10.1073/pnas.0405816101</identifier><identifier>PMID: 15534229</identifier><language>eng</language><publisher>United States: National Academy of Sciences</publisher><subject>Animals ; Antibodies ; Aujeszky disease ; Base Sequence ; Biological Sciences ; Cell Adhesion Molecules - chemistry ; Cell Adhesion Molecules - genetics ; Cell Adhesion Molecules - physiology ; Cellular Biology ; disease resistance ; DNA, Viral - genetics ; DNA, Viral - isolation & purification ; Fibroblasts ; Gene expression ; gene transfer ; Genetic engineering ; genetic resistance ; Herpes simplex virus ; Herpesviridae - pathogenicity ; Herpesvirus ; Herpesvirus 1, Suid - genetics ; Herpesvirus 1, Suid - isolation & purification ; Herpesvirus 1, Suid - pathogenicity ; Human herpesvirus 1 ; Humans ; Immunoglobulin Fc Fragments - genetics ; Immunoglobulin Fc Fragments - metabolism ; Immunoglobulin G - genetics ; Immunoglobulin G - metabolism ; Infections ; Lethal dose 50 ; Life Sciences ; Mice ; Mice, Inbred C57BL ; Mice, Transgenic ; Models, Biological ; nectin-1 ; Nectins ; Polymerase Chain Reaction ; Pseudorabies - immunology ; Pseudorabies - prevention & control ; Pseudorabies - virology ; Pseudorabies virus ; receptors ; Receptors, Virus - chemistry ; Receptors, Virus - genetics ; Receptors, Virus - physiology ; Recombinant Proteins - genetics ; Recombinant Proteins - metabolism ; Rodents ; Solubility ; soluble receptors ; Suid herpesvirus 1 ; Sus scrofa ; swine ; Transgenes ; Transgenic animals ; Virology ; Viruses</subject><ispartof>Proceedings of the National Academy of Sciences - PNAS, 2004-11, Vol.101 (46), p.16150-16155</ispartof><rights>Copyright 1993/2004 The National Academy of Sciences of the United States of America</rights><rights>Copyright National Academy of Sciences Nov 16, 2004</rights><rights>Attribution - ShareAlike</rights><rights>Copyright © 2004, The National Academy of Sciences 2004</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c650t-41fb4f14f2b194330d7c1dfaca5b72ff8fc5f15b308359d90a75977e7dcf902a3</citedby><cites>FETCH-LOGICAL-c650t-41fb4f14f2b194330d7c1dfaca5b72ff8fc5f15b308359d90a75977e7dcf902a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.pnas.org/content/101/46.cover.gif</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/3373787$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/3373787$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,723,776,780,799,881,27903,27904,53770,53772,57996,58229</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15534229$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://hal.inrae.fr/hal-02679869$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Ono, E</creatorcontrib><creatorcontrib>Amagai, K</creatorcontrib><creatorcontrib>Taharaguchi, S</creatorcontrib><creatorcontrib>Tomioka, Y</creatorcontrib><creatorcontrib>Yoshino, S</creatorcontrib><creatorcontrib>Watanabe, Y</creatorcontrib><creatorcontrib>Cherel, P</creatorcontrib><creatorcontrib>Houdebine, L.M</creatorcontrib><creatorcontrib>Adam, M</creatorcontrib><creatorcontrib>Eloit, M</creatorcontrib><title>Transgenic mice expressing a soluble form of porcine nectin-1/herpesvirus entry mediator C as a model for pseudorabies-resistant livestock</title><title>Proceedings of the National Academy of Sciences - PNAS</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>An approach to genetically engineered resistance to pseudorabies virus (PRV) infection was examined by using a transgene encoding a soluble form of nectin-1, also known as herpesvirus entry mediator C. Nectin-1 is an alpha-herpesvirus receptor that binds to virion glycoprotein D. Nectin-1 mediates entry of PRV, herpes simplex virus types 1 and 2, and bovine herpesvirus type 1. To assess the antiviral potential of an ectopic expression of the nectin-1 ectodomain in vivo, six transgenic mouse lines expressing a soluble form of nectin-1, consisting of an extracellular domain of porcine nectin-1 and the Fc portion of human IgG1, were generated. All of the transgenic mouse lines showed nearly complete resistance to PRV infection by means of both i.p. and intranasal routes. These results suggest that the introduction into farm animals of a transgene encoding a soluble form of nectin-1 would offer a potent biological approach to generating alpha-herpesvirus-resistant livestock.</description><subject>Animals</subject><subject>Antibodies</subject><subject>Aujeszky disease</subject><subject>Base Sequence</subject><subject>Biological Sciences</subject><subject>Cell Adhesion Molecules - chemistry</subject><subject>Cell Adhesion Molecules - genetics</subject><subject>Cell Adhesion Molecules - physiology</subject><subject>Cellular Biology</subject><subject>disease resistance</subject><subject>DNA, Viral - genetics</subject><subject>DNA, Viral - isolation & purification</subject><subject>Fibroblasts</subject><subject>Gene expression</subject><subject>gene transfer</subject><subject>Genetic engineering</subject><subject>genetic resistance</subject><subject>Herpes simplex virus</subject><subject>Herpesviridae - pathogenicity</subject><subject>Herpesvirus</subject><subject>Herpesvirus 1, Suid - genetics</subject><subject>Herpesvirus 1, Suid - isolation & purification</subject><subject>Herpesvirus 1, Suid - pathogenicity</subject><subject>Human herpesvirus 1</subject><subject>Humans</subject><subject>Immunoglobulin Fc Fragments - genetics</subject><subject>Immunoglobulin Fc Fragments - metabolism</subject><subject>Immunoglobulin G - genetics</subject><subject>Immunoglobulin G - metabolism</subject><subject>Infections</subject><subject>Lethal dose 50</subject><subject>Life Sciences</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>Mice, Transgenic</subject><subject>Models, Biological</subject><subject>nectin-1</subject><subject>Nectins</subject><subject>Polymerase Chain Reaction</subject><subject>Pseudorabies - immunology</subject><subject>Pseudorabies - prevention & control</subject><subject>Pseudorabies - virology</subject><subject>Pseudorabies virus</subject><subject>receptors</subject><subject>Receptors, Virus - chemistry</subject><subject>Receptors, Virus - genetics</subject><subject>Receptors, Virus - physiology</subject><subject>Recombinant Proteins - genetics</subject><subject>Recombinant Proteins - metabolism</subject><subject>Rodents</subject><subject>Solubility</subject><subject>soluble receptors</subject><subject>Suid herpesvirus 1</subject><subject>Sus scrofa</subject><subject>swine</subject><subject>Transgenes</subject><subject>Transgenic animals</subject><subject>Virology</subject><subject>Viruses</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkkFv1DAQhSMEokvhzAWBxQGJQ7rj2I7jA4dqBRRpJQ60Z8tx7F0vSRzsZNX-BX41DrvqQi89WfJ873lm_LLsNYYLDJwsh17FC6DAKlxiwE-yBQaB85IKeJotAAqeV7SgZ9mLGHcAIFgFz7MzzBihRSEW2e_roPq4Mb3TqHPaIHM7BBOj6zdIoejbqW4Nsj50yFs0-KBdb1Bv9Oj6HC-3Jgwm7l2YIjL9GO5QZxqnRh_QCqmYLDrfmHY2QEM0U-ODqp2JeXrDxVH1I2rd3sTR658vs2dWtdG8Op7n2c2Xz9erq3z9_eu31eU61yWDMafY1tRiaosaC0oINFzjxiqtWM0LayurmcWsJlARJhoBijPBueGNtgIKRc6zTwffYapTt3ruW7VyCK5T4U565eT_ld5t5cbvJSsqwSDpPx702weqq8u1nO-gKLmoSrHHif1wfCv4X1OaU3YuatO2qjd-irLkUFFB4FEQc17iisyO7x-AOz-FPi1MFoAJpSWwBC0PkA4-xmDsfZ8Y5BwcOQdHnoKTFG__XcqJPyblNPVf5ckOS1rK5MFA2qltR3M7JhY9wibkzQHZpb8P9wwhnPCKp_K7Q9kqL9UmuChvfszzpQhXHNIEfwCLQuyH</recordid><startdate>20041116</startdate><enddate>20041116</enddate><creator>Ono, E</creator><creator>Amagai, K</creator><creator>Taharaguchi, S</creator><creator>Tomioka, Y</creator><creator>Yoshino, S</creator><creator>Watanabe, Y</creator><creator>Cherel, P</creator><creator>Houdebine, L.M</creator><creator>Adam, M</creator><creator>Eloit, M</creator><general>National Academy of Sciences</general><general>National Acad Sciences</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QG</scope><scope>7QL</scope><scope>7QP</scope><scope>7QR</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TK</scope><scope>7TM</scope><scope>7TO</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7QO</scope><scope>7X8</scope><scope>1XC</scope><scope>VOOES</scope><scope>5PM</scope></search><sort><creationdate>20041116</creationdate><title>Transgenic mice expressing a soluble form of porcine nectin-1/herpesvirus entry mediator C as a model for pseudorabies-resistant livestock</title><author>Ono, E ; Amagai, K ; Taharaguchi, S ; Tomioka, Y ; Yoshino, S ; Watanabe, Y ; Cherel, P ; Houdebine, L.M ; Adam, M ; Eloit, M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c650t-41fb4f14f2b194330d7c1dfaca5b72ff8fc5f15b308359d90a75977e7dcf902a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Animals</topic><topic>Antibodies</topic><topic>Aujeszky disease</topic><topic>Base Sequence</topic><topic>Biological Sciences</topic><topic>Cell Adhesion Molecules - chemistry</topic><topic>Cell Adhesion Molecules - genetics</topic><topic>Cell Adhesion Molecules - physiology</topic><topic>Cellular Biology</topic><topic>disease resistance</topic><topic>DNA, Viral - genetics</topic><topic>DNA, Viral - isolation & purification</topic><topic>Fibroblasts</topic><topic>Gene expression</topic><topic>gene transfer</topic><topic>Genetic engineering</topic><topic>genetic resistance</topic><topic>Herpes simplex virus</topic><topic>Herpesviridae - pathogenicity</topic><topic>Herpesvirus</topic><topic>Herpesvirus 1, Suid - genetics</topic><topic>Herpesvirus 1, Suid - isolation & purification</topic><topic>Herpesvirus 1, Suid - pathogenicity</topic><topic>Human herpesvirus 1</topic><topic>Humans</topic><topic>Immunoglobulin Fc Fragments - genetics</topic><topic>Immunoglobulin Fc Fragments - metabolism</topic><topic>Immunoglobulin G - genetics</topic><topic>Immunoglobulin G - metabolism</topic><topic>Infections</topic><topic>Lethal dose 50</topic><topic>Life Sciences</topic><topic>Mice</topic><topic>Mice, Inbred C57BL</topic><topic>Mice, Transgenic</topic><topic>Models, Biological</topic><topic>nectin-1</topic><topic>Nectins</topic><topic>Polymerase Chain Reaction</topic><topic>Pseudorabies - immunology</topic><topic>Pseudorabies - prevention & control</topic><topic>Pseudorabies - virology</topic><topic>Pseudorabies virus</topic><topic>receptors</topic><topic>Receptors, Virus - chemistry</topic><topic>Receptors, Virus - genetics</topic><topic>Receptors, Virus - physiology</topic><topic>Recombinant Proteins - genetics</topic><topic>Recombinant Proteins - metabolism</topic><topic>Rodents</topic><topic>Solubility</topic><topic>soluble receptors</topic><topic>Suid herpesvirus 1</topic><topic>Sus scrofa</topic><topic>swine</topic><topic>Transgenes</topic><topic>Transgenic animals</topic><topic>Virology</topic><topic>Viruses</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ono, E</creatorcontrib><creatorcontrib>Amagai, K</creatorcontrib><creatorcontrib>Taharaguchi, S</creatorcontrib><creatorcontrib>Tomioka, Y</creatorcontrib><creatorcontrib>Yoshino, S</creatorcontrib><creatorcontrib>Watanabe, Y</creatorcontrib><creatorcontrib>Cherel, P</creatorcontrib><creatorcontrib>Houdebine, L.M</creatorcontrib><creatorcontrib>Adam, M</creatorcontrib><creatorcontrib>Eloit, M</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>MEDLINE - Academic</collection><collection>Hyper Article en Ligne (HAL)</collection><collection>Hyper Article en Ligne (HAL) (Open Access)</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ono, E</au><au>Amagai, K</au><au>Taharaguchi, S</au><au>Tomioka, Y</au><au>Yoshino, S</au><au>Watanabe, Y</au><au>Cherel, P</au><au>Houdebine, L.M</au><au>Adam, M</au><au>Eloit, M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Transgenic mice expressing a soluble form of porcine nectin-1/herpesvirus entry mediator C as a model for pseudorabies-resistant livestock</atitle><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle><addtitle>Proc Natl Acad Sci U S A</addtitle><date>2004-11-16</date><risdate>2004</risdate><volume>101</volume><issue>46</issue><spage>16150</spage><epage>16155</epage><pages>16150-16155</pages><issn>0027-8424</issn><eissn>1091-6490</eissn><abstract>An approach to genetically engineered resistance to pseudorabies virus (PRV) infection was examined by using a transgene encoding a soluble form of nectin-1, also known as herpesvirus entry mediator C. Nectin-1 is an alpha-herpesvirus receptor that binds to virion glycoprotein D. Nectin-1 mediates entry of PRV, herpes simplex virus types 1 and 2, and bovine herpesvirus type 1. To assess the antiviral potential of an ectopic expression of the nectin-1 ectodomain in vivo, six transgenic mouse lines expressing a soluble form of nectin-1, consisting of an extracellular domain of porcine nectin-1 and the Fc portion of human IgG1, were generated. All of the transgenic mouse lines showed nearly complete resistance to PRV infection by means of both i.p. and intranasal routes. These results suggest that the introduction into farm animals of a transgene encoding a soluble form of nectin-1 would offer a potent biological approach to generating alpha-herpesvirus-resistant livestock.</abstract><cop>United States</cop><pub>National Academy of Sciences</pub><pmid>15534229</pmid><doi>10.1073/pnas.0405816101</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Animals Antibodies Aujeszky disease Base Sequence Biological Sciences Cell Adhesion Molecules - chemistry Cell Adhesion Molecules - genetics Cell Adhesion Molecules - physiology Cellular Biology disease resistance DNA, Viral - genetics DNA, Viral - isolation & purification Fibroblasts Gene expression gene transfer Genetic engineering genetic resistance Herpes simplex virus Herpesviridae - pathogenicity Herpesvirus Herpesvirus 1, Suid - genetics Herpesvirus 1, Suid - isolation & purification Herpesvirus 1, Suid - pathogenicity Human herpesvirus 1 Humans Immunoglobulin Fc Fragments - genetics Immunoglobulin Fc Fragments - metabolism Immunoglobulin G - genetics Immunoglobulin G - metabolism Infections Lethal dose 50 Life Sciences Mice Mice, Inbred C57BL Mice, Transgenic Models, Biological nectin-1 Nectins Polymerase Chain Reaction Pseudorabies - immunology Pseudorabies - prevention & control Pseudorabies - virology Pseudorabies virus receptors Receptors, Virus - chemistry Receptors, Virus - genetics Receptors, Virus - physiology Recombinant Proteins - genetics Recombinant Proteins - metabolism Rodents Solubility soluble receptors Suid herpesvirus 1 Sus scrofa swine Transgenes Transgenic animals Virology Viruses |
| title | Transgenic mice expressing a soluble form of porcine nectin-1/herpesvirus entry mediator C as a model for pseudorabies-resistant livestock |
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