Three Cadherin Alleles Associated with Resistance to Bacillus thuringiensis in Pink Bollworm
Evolution of resistance by pests is the main threat to long-term insect control by transgenic crops that produce Bacillus thuringiensis (Bt) toxins. Because inheritance of resistance to the Bt toxins in transgenic crops is typically recessive, DNA-based screening for resistance alleles in heterozygo...
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| Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 2003-04, Vol.100 (9), p.5004-5009 |
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| creator | Morin, Shai Biggs, Robert W. Sisterson, Mark S. Shriver, Laura Ellers-Kirk, Christa Higginson, Dawn Holley, Daniel Gahan, Linda J. Heckel, David G. Carrière, Yves Dennehy, Timothy J. Brown, Judith K. Tabashnik, Bruce E. |
| description | Evolution of resistance by pests is the main threat to long-term insect control by transgenic crops that produce Bacillus thuringiensis (Bt) toxins. Because inheritance of resistance to the Bt toxins in transgenic crops is typically recessive, DNA-based screening for resistance alleles in heterozygotes is potentially much more efficient than detection of resistant homozygotes with bioassays. Such screening, however, requires knowledge of the resistance alleles in field populations of pests that are associated with survival on Bt crops. Here we report that field populations of pink bollworm (Pectinophora gossypiella), a major cotton pest, harbored three mutant alleles of a cadherin-encoding gene linked with resistance to Bt toxin Cry1Ac and survival on transgenic Bt cotton. Each of the three resistance alleles has a deletion expected to eliminate at least eight amino acids upstream of the putative toxin-binding region of the cadherin protein. Larvae with two resistance alleles in any combination were resistant, whereas those with one or none were susceptible to Cry1Ac. Together with previous evidence, the results reported here identify the cadherin gene as a leading target for DNA-based screening of resistance to Bt crops in lepidopteran pests. |
| doi_str_mv | 10.1073/pnas.0831036100 |
| format | Article |
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Because inheritance of resistance to the Bt toxins in transgenic crops is typically recessive, DNA-based screening for resistance alleles in heterozygotes is potentially much more efficient than detection of resistant homozygotes with bioassays. Such screening, however, requires knowledge of the resistance alleles in field populations of pests that are associated with survival on Bt crops. Here we report that field populations of pink bollworm (Pectinophora gossypiella), a major cotton pest, harbored three mutant alleles of a cadherin-encoding gene linked with resistance to Bt toxin Cry1Ac and survival on transgenic Bt cotton. Each of the three resistance alleles has a deletion expected to eliminate at least eight amino acids upstream of the putative toxin-binding region of the cadherin protein. Larvae with two resistance alleles in any combination were resistant, whereas those with one or none were susceptible to Cry1Ac. Together with previous evidence, the results reported here identify the cadherin gene as a leading target for DNA-based screening of resistance to Bt crops in lepidopteran pests.</description><identifier>ISSN: 0027-8424</identifier><identifier>EISSN: 1091-6490</identifier><identifier>DOI: 10.1073/pnas.0831036100</identifier><identifier>PMID: 12695565</identifier><language>eng</language><publisher>United States: National Academy of Sciences</publisher><subject>Alleles ; Amino Acid Sequence ; Animals ; Bacillus thuringiensis - pathogenicity ; Bioassay ; Biological Assay ; Biological Sciences ; Bollworms ; Cadherins ; Cadherins - chemistry ; Cadherins - genetics ; Cotton ; Diet ; Female ; Genes ; Genetic Linkage ; Genetic Predisposition to Disease ; Genotypes ; Insect larvae ; Larvae ; Lepidoptera - genetics ; Lepidoptera - microbiology ; Male ; Molecular Sequence Data ; Mutation ; Proteins ; Reverse Transcriptase Polymerase Chain Reaction ; Sequence Homology, Amino Acid ; Toxins</subject><ispartof>Proceedings of the National Academy of Sciences - PNAS, 2003-04, Vol.100 (9), p.5004-5009</ispartof><rights>Copyright 1993-2003 National Academy of Sciences of the United States of America</rights><rights>Copyright National Academy of Sciences Apr 29, 2003</rights><rights>Copyright © 2003, The National Academy of Sciences 2003</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c522t-59d606722e9fbd97caed106ba263b46f3edc3f1b8639965dae5ef3926fad6e533</citedby><cites>FETCH-LOGICAL-c522t-59d606722e9fbd97caed106ba263b46f3edc3f1b8639965dae5ef3926fad6e533</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.pnas.org/content/100/9.cover.gif</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/3139655$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/3139655$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,723,776,780,799,881,27903,27904,53770,53772,57996,58229</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12695565$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Morin, Shai</creatorcontrib><creatorcontrib>Biggs, Robert W.</creatorcontrib><creatorcontrib>Sisterson, Mark S.</creatorcontrib><creatorcontrib>Shriver, Laura</creatorcontrib><creatorcontrib>Ellers-Kirk, Christa</creatorcontrib><creatorcontrib>Higginson, Dawn</creatorcontrib><creatorcontrib>Holley, Daniel</creatorcontrib><creatorcontrib>Gahan, Linda J.</creatorcontrib><creatorcontrib>Heckel, David G.</creatorcontrib><creatorcontrib>Carrière, Yves</creatorcontrib><creatorcontrib>Dennehy, Timothy J.</creatorcontrib><creatorcontrib>Brown, Judith K.</creatorcontrib><creatorcontrib>Tabashnik, Bruce E.</creatorcontrib><title>Three Cadherin Alleles Associated with Resistance to Bacillus thuringiensis in Pink Bollworm</title><title>Proceedings of the National Academy of Sciences - PNAS</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>Evolution of resistance by pests is the main threat to long-term insect control by transgenic crops that produce Bacillus thuringiensis (Bt) toxins. 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Together with previous evidence, the results reported here identify the cadherin gene as a leading target for DNA-based screening of resistance to Bt crops in lepidopteran pests.</description><subject>Alleles</subject><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Bacillus thuringiensis - pathogenicity</subject><subject>Bioassay</subject><subject>Biological Assay</subject><subject>Biological Sciences</subject><subject>Bollworms</subject><subject>Cadherins</subject><subject>Cadherins - chemistry</subject><subject>Cadherins - genetics</subject><subject>Cotton</subject><subject>Diet</subject><subject>Female</subject><subject>Genes</subject><subject>Genetic Linkage</subject><subject>Genetic Predisposition to Disease</subject><subject>Genotypes</subject><subject>Insect larvae</subject><subject>Larvae</subject><subject>Lepidoptera - genetics</subject><subject>Lepidoptera - microbiology</subject><subject>Male</subject><subject>Molecular Sequence Data</subject><subject>Mutation</subject><subject>Proteins</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>Sequence Homology, Amino Acid</subject><subject>Toxins</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU1v1DAURS0EokNhzQahiAWs0j7bsRMvWExHfEmVQKjskCwneWk8eOLBdij8ezyaUaewgJUX75zna19CnlI4o1Dz8-1k4hk0nAKXFOAeWVBQtJSVgvtkAcDqsqlYdUIexbgGACUaeEhOKJNKCCkW5OvVGBCLlelHDHYqls6hw1gsY_SdNQn74samsfiM0cZkpg6L5IsL01nn5likcc7WtcUpj4vsf7LTt-LCO3fjw-YxeTAYF_HJ4TwlX96-uVq9Ly8_vvuwWl6WnWAslUL1EmTNGKqh7VXdGewpyNYwydtKDhz7jg-0bSRXSoreoMCBKyYH00sUnJ-S1_u927ndZBinFIzT22A3JvzS3lj952Syo772PzQVFWua7L88-MF_nzEmvbGxQ-fMhH6OuuaM5evq_4K0qXkjlMrgi7_AtZ_DlD9BM6BcCaZ2sc_3UBd8jAGH28QU9K5evatXH-vNxvO7Dz3yhz7vADvzuA600gKgysCrfwJ6mJ1L-DNl8tmeXMfkwy3Kc3opBP8NRTPDmw</recordid><startdate>20030429</startdate><enddate>20030429</enddate><creator>Morin, Shai</creator><creator>Biggs, Robert W.</creator><creator>Sisterson, Mark S.</creator><creator>Shriver, Laura</creator><creator>Ellers-Kirk, Christa</creator><creator>Higginson, Dawn</creator><creator>Holley, Daniel</creator><creator>Gahan, Linda J.</creator><creator>Heckel, David G.</creator><creator>Carrière, Yves</creator><creator>Dennehy, Timothy J.</creator><creator>Brown, Judith K.</creator><creator>Tabashnik, Bruce E.</creator><general>National Academy of Sciences</general><general>National Acad Sciences</general><general>The National Academy of Sciences</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QG</scope><scope>7QL</scope><scope>7QP</scope><scope>7QR</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TK</scope><scope>7TM</scope><scope>7TO</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7QO</scope><scope>7T7</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20030429</creationdate><title>Three Cadherin Alleles Associated with Resistance to Bacillus thuringiensis in Pink Bollworm</title><author>Morin, Shai ; 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Because inheritance of resistance to the Bt toxins in transgenic crops is typically recessive, DNA-based screening for resistance alleles in heterozygotes is potentially much more efficient than detection of resistant homozygotes with bioassays. Such screening, however, requires knowledge of the resistance alleles in field populations of pests that are associated with survival on Bt crops. Here we report that field populations of pink bollworm (Pectinophora gossypiella), a major cotton pest, harbored three mutant alleles of a cadherin-encoding gene linked with resistance to Bt toxin Cry1Ac and survival on transgenic Bt cotton. Each of the three resistance alleles has a deletion expected to eliminate at least eight amino acids upstream of the putative toxin-binding region of the cadherin protein. Larvae with two resistance alleles in any combination were resistant, whereas those with one or none were susceptible to Cry1Ac. 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| subjects | Alleles Amino Acid Sequence Animals Bacillus thuringiensis - pathogenicity Bioassay Biological Assay Biological Sciences Bollworms Cadherins Cadherins - chemistry Cadherins - genetics Cotton Diet Female Genes Genetic Linkage Genetic Predisposition to Disease Genotypes Insect larvae Larvae Lepidoptera - genetics Lepidoptera - microbiology Male Molecular Sequence Data Mutation Proteins Reverse Transcriptase Polymerase Chain Reaction Sequence Homology, Amino Acid Toxins |
| title | Three Cadherin Alleles Associated with Resistance to Bacillus thuringiensis in Pink Bollworm |
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