mRNA of Placental Origin Is Readily Detectable in Maternal Plasma
The discovery of circulating fetal nucleic acid in maternal plasma has opened up new possibilities for noninvasive prenatal diagnosis. Thus far, a gender- and polymorphism-independent fetal-specific target that can be used for prenatal screening and monitoring in all pregnant women has not been repo...
Gespeichert in:
| Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 2003-04, Vol.100 (8), p.4748-4753 |
|---|---|
| Hauptverfasser: | , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 4753 |
|---|---|
| container_issue | 8 |
| container_start_page | 4748 |
| container_title | Proceedings of the National Academy of Sciences - PNAS |
| container_volume | 100 |
| creator | Enders K. O. Ng Nancy B. Y. Tsui Lau, Tze K. Leung, Tse N. Rossa W. K. Chiu Panesar, Nirmal S. Lydia C. W. Lit Chan, Kam-Wing Y. M. Dennis Lo |
| description | The discovery of circulating fetal nucleic acid in maternal plasma has opened up new possibilities for noninvasive prenatal diagnosis. Thus far, a gender- and polymorphism-independent fetal-specific target that can be used for prenatal screening and monitoring in all pregnant women has not been reported. In addition, the origin of such circulating nucleic acid has remained unclear. Here we provide direct evidence that the placenta is an important source of fetal nucleic acid release into maternal plasma by demonstrating that mRNA transcripts from placenta-expressed genes are readily detectable in maternal plasma. The surprising stability of such placental mRNA species in maternal plasma and their rapid clearance after delivery demonstrate that such circulating mRNA molecules are practical markers for clinical use. The measurement of such plasma mRNA markers has provided a gender-independent approach for noninvasive prenatal gene expression profiling and has opened up numerous research and diagnostic possibilities. |
| doi_str_mv | 10.1073/pnas.0637450100 |
| format | Article |
| fullrecord | <record><control><sourceid>jstor_pnas_</sourceid><recordid>TN_cdi_pnas_primary_100_8_4748</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><jstor_id>3144013</jstor_id><sourcerecordid>3144013</sourcerecordid><originalsourceid>FETCH-LOGICAL-c588t-7e7e94a2ed68953b05fb381ffcc69cc68c3cdbaf8717e21a0fcc4a91dd3c66c63</originalsourceid><addsrcrecordid>eNqFkUtP3DAUhS3Uqkxp191UKGLRrgLXj_ixYDGiLyQoFSpry3EcyMhJBtupyr-voxkxtAu6sLy43zk69x6E3mE4xiDoyXow8Rg4FawCDLCHFhgULjlT8AItAIgoJSNsH72OcQUAqpLwCu1jwhkToBZo2V9_XxZjW_zwxrohGV9che62G4rzWFw703T-ofjkkrPJ1N4VeXBpkgtDBrMk9uYNetkaH93b7X-Abr58_nn2rby4-np-trwobSVlKoUTTjFDXMOlqmgNVVtTidvWWq7yk5bapjatFFg4gg3kATMKNw21nFtOD9Dpxnc91b1r5rDBeL0OXW_Cgx5Np_-eDN2dvh1_aVxRTkTWf9jqw3g_uZh030XrvDeDG6eoBSVAKcB_QSwF4VTKDB79A67Gab5M1AQwFRVXs9vJBrJhjDG49jExBj13qOcO9a7DrDh8uuiO35b2BJiVOzvQUjPB5mAfnwV0O3mf3O-UyfcbchXTGB5RihnLG9A_NCq4uw</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>201375690</pqid></control><display><type>article</type><title>mRNA of Placental Origin Is Readily Detectable in Maternal Plasma</title><source>MEDLINE</source><source>Jstor Complete Legacy</source><source>PubMed Central</source><source>Alma/SFX Local Collection</source><source>Free Full-Text Journals in Chemistry</source><creator>Enders K. O. Ng ; Nancy B. Y. Tsui ; Lau, Tze K. ; Leung, Tse N. ; Rossa W. K. Chiu ; Panesar, Nirmal S. ; Lydia C. W. Lit ; Chan, Kam-Wing ; Y. M. Dennis Lo</creator><creatorcontrib>Enders K. O. Ng ; Nancy B. Y. Tsui ; Lau, Tze K. ; Leung, Tse N. ; Rossa W. K. Chiu ; Panesar, Nirmal S. ; Lydia C. W. Lit ; Chan, Kam-Wing ; Y. M. Dennis Lo</creatorcontrib><description>The discovery of circulating fetal nucleic acid in maternal plasma has opened up new possibilities for noninvasive prenatal diagnosis. Thus far, a gender- and polymorphism-independent fetal-specific target that can be used for prenatal screening and monitoring in all pregnant women has not been reported. In addition, the origin of such circulating nucleic acid has remained unclear. Here we provide direct evidence that the placenta is an important source of fetal nucleic acid release into maternal plasma by demonstrating that mRNA transcripts from placenta-expressed genes are readily detectable in maternal plasma. The surprising stability of such placental mRNA species in maternal plasma and their rapid clearance after delivery demonstrate that such circulating mRNA molecules are practical markers for clinical use. The measurement of such plasma mRNA markers has provided a gender-independent approach for noninvasive prenatal gene expression profiling and has opened up numerous research and diagnostic possibilities.</description><identifier>ISSN: 0027-8424</identifier><identifier>EISSN: 1091-6490</identifier><identifier>DOI: 10.1073/pnas.0637450100</identifier><identifier>PMID: 12644709</identifier><language>eng</language><publisher>United States: National Academy of Sciences</publisher><subject>Base Sequence ; Biological Sciences ; Biological Transport, Active ; Blood ; Blood plasma ; DNA ; DNA Primers - genetics ; Female ; Fetus - metabolism ; Fetuses ; Gene Expression Profiling ; Gestational Age ; Humans ; Maternal-Fetal Exchange - genetics ; Medical research ; Messenger RNA ; Nucleic acids ; Placenta ; Placenta - metabolism ; Placental Lactogen - genetics ; Plasma ; Plasma stability ; Pregnancy ; Prenatal Diagnosis ; Reverse Transcriptase Polymerase Chain Reaction ; Ribonucleic acid ; RNA ; RNA Stability ; RNA, Messenger - blood ; RNA, Messenger - genetics ; RNA, Messenger - metabolism</subject><ispartof>Proceedings of the National Academy of Sciences - PNAS, 2003-04, Vol.100 (8), p.4748-4753</ispartof><rights>Copyright 1993-2003 National Academy of Sciences of the United States of America</rights><rights>Copyright National Academy of Sciences Apr 15, 2003</rights><rights>Copyright © 2003, The National Academy of Sciences 2003</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c588t-7e7e94a2ed68953b05fb381ffcc69cc68c3cdbaf8717e21a0fcc4a91dd3c66c63</citedby><cites>FETCH-LOGICAL-c588t-7e7e94a2ed68953b05fb381ffcc69cc68c3cdbaf8717e21a0fcc4a91dd3c66c63</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.pnas.org/content/100/8.cover.gif</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/3144013$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/3144013$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,724,777,781,800,882,27905,27906,53772,53774,57998,58231</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12644709$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Enders K. O. Ng</creatorcontrib><creatorcontrib>Nancy B. Y. Tsui</creatorcontrib><creatorcontrib>Lau, Tze K.</creatorcontrib><creatorcontrib>Leung, Tse N.</creatorcontrib><creatorcontrib>Rossa W. K. Chiu</creatorcontrib><creatorcontrib>Panesar, Nirmal S.</creatorcontrib><creatorcontrib>Lydia C. W. Lit</creatorcontrib><creatorcontrib>Chan, Kam-Wing</creatorcontrib><creatorcontrib>Y. M. Dennis Lo</creatorcontrib><title>mRNA of Placental Origin Is Readily Detectable in Maternal Plasma</title><title>Proceedings of the National Academy of Sciences - PNAS</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>The discovery of circulating fetal nucleic acid in maternal plasma has opened up new possibilities for noninvasive prenatal diagnosis. Thus far, a gender- and polymorphism-independent fetal-specific target that can be used for prenatal screening and monitoring in all pregnant women has not been reported. In addition, the origin of such circulating nucleic acid has remained unclear. Here we provide direct evidence that the placenta is an important source of fetal nucleic acid release into maternal plasma by demonstrating that mRNA transcripts from placenta-expressed genes are readily detectable in maternal plasma. The surprising stability of such placental mRNA species in maternal plasma and their rapid clearance after delivery demonstrate that such circulating mRNA molecules are practical markers for clinical use. The measurement of such plasma mRNA markers has provided a gender-independent approach for noninvasive prenatal gene expression profiling and has opened up numerous research and diagnostic possibilities.</description><subject>Base Sequence</subject><subject>Biological Sciences</subject><subject>Biological Transport, Active</subject><subject>Blood</subject><subject>Blood plasma</subject><subject>DNA</subject><subject>DNA Primers - genetics</subject><subject>Female</subject><subject>Fetus - metabolism</subject><subject>Fetuses</subject><subject>Gene Expression Profiling</subject><subject>Gestational Age</subject><subject>Humans</subject><subject>Maternal-Fetal Exchange - genetics</subject><subject>Medical research</subject><subject>Messenger RNA</subject><subject>Nucleic acids</subject><subject>Placenta</subject><subject>Placenta - metabolism</subject><subject>Placental Lactogen - genetics</subject><subject>Plasma</subject><subject>Plasma stability</subject><subject>Pregnancy</subject><subject>Prenatal Diagnosis</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>Ribonucleic acid</subject><subject>RNA</subject><subject>RNA Stability</subject><subject>RNA, Messenger - blood</subject><subject>RNA, Messenger - genetics</subject><subject>RNA, Messenger - metabolism</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUtP3DAUhS3Uqkxp191UKGLRrgLXj_ixYDGiLyQoFSpry3EcyMhJBtupyr-voxkxtAu6sLy43zk69x6E3mE4xiDoyXow8Rg4FawCDLCHFhgULjlT8AItAIgoJSNsH72OcQUAqpLwCu1jwhkToBZo2V9_XxZjW_zwxrohGV9che62G4rzWFw703T-ofjkkrPJ1N4VeXBpkgtDBrMk9uYNetkaH93b7X-Abr58_nn2rby4-np-trwobSVlKoUTTjFDXMOlqmgNVVtTidvWWq7yk5bapjatFFg4gg3kATMKNw21nFtOD9Dpxnc91b1r5rDBeL0OXW_Cgx5Np_-eDN2dvh1_aVxRTkTWf9jqw3g_uZh030XrvDeDG6eoBSVAKcB_QSwF4VTKDB79A67Gab5M1AQwFRVXs9vJBrJhjDG49jExBj13qOcO9a7DrDh8uuiO35b2BJiVOzvQUjPB5mAfnwV0O3mf3O-UyfcbchXTGB5RihnLG9A_NCq4uw</recordid><startdate>20030415</startdate><enddate>20030415</enddate><creator>Enders K. O. Ng</creator><creator>Nancy B. Y. Tsui</creator><creator>Lau, Tze K.</creator><creator>Leung, Tse N.</creator><creator>Rossa W. K. Chiu</creator><creator>Panesar, Nirmal S.</creator><creator>Lydia C. W. Lit</creator><creator>Chan, Kam-Wing</creator><creator>Y. M. Dennis Lo</creator><general>National Academy of Sciences</general><general>National Acad Sciences</general><general>The National Academy of Sciences</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QG</scope><scope>7QL</scope><scope>7QP</scope><scope>7QR</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TK</scope><scope>7TM</scope><scope>7TO</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20030415</creationdate><title>mRNA of Placental Origin Is Readily Detectable in Maternal Plasma</title><author>Enders K. O. Ng ; Nancy B. Y. Tsui ; Lau, Tze K. ; Leung, Tse N. ; Rossa W. K. Chiu ; Panesar, Nirmal S. ; Lydia C. W. Lit ; Chan, Kam-Wing ; Y. M. Dennis Lo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c588t-7e7e94a2ed68953b05fb381ffcc69cc68c3cdbaf8717e21a0fcc4a91dd3c66c63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Base Sequence</topic><topic>Biological Sciences</topic><topic>Biological Transport, Active</topic><topic>Blood</topic><topic>Blood plasma</topic><topic>DNA</topic><topic>DNA Primers - genetics</topic><topic>Female</topic><topic>Fetus - metabolism</topic><topic>Fetuses</topic><topic>Gene Expression Profiling</topic><topic>Gestational Age</topic><topic>Humans</topic><topic>Maternal-Fetal Exchange - genetics</topic><topic>Medical research</topic><topic>Messenger RNA</topic><topic>Nucleic acids</topic><topic>Placenta</topic><topic>Placenta - metabolism</topic><topic>Placental Lactogen - genetics</topic><topic>Plasma</topic><topic>Plasma stability</topic><topic>Pregnancy</topic><topic>Prenatal Diagnosis</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>Ribonucleic acid</topic><topic>RNA</topic><topic>RNA Stability</topic><topic>RNA, Messenger - blood</topic><topic>RNA, Messenger - genetics</topic><topic>RNA, Messenger - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Enders K. O. Ng</creatorcontrib><creatorcontrib>Nancy B. Y. Tsui</creatorcontrib><creatorcontrib>Lau, Tze K.</creatorcontrib><creatorcontrib>Leung, Tse N.</creatorcontrib><creatorcontrib>Rossa W. K. Chiu</creatorcontrib><creatorcontrib>Panesar, Nirmal S.</creatorcontrib><creatorcontrib>Lydia C. W. Lit</creatorcontrib><creatorcontrib>Chan, Kam-Wing</creatorcontrib><creatorcontrib>Y. M. Dennis Lo</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Enders K. O. Ng</au><au>Nancy B. Y. Tsui</au><au>Lau, Tze K.</au><au>Leung, Tse N.</au><au>Rossa W. K. Chiu</au><au>Panesar, Nirmal S.</au><au>Lydia C. W. Lit</au><au>Chan, Kam-Wing</au><au>Y. M. Dennis Lo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>mRNA of Placental Origin Is Readily Detectable in Maternal Plasma</atitle><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle><addtitle>Proc Natl Acad Sci U S A</addtitle><date>2003-04-15</date><risdate>2003</risdate><volume>100</volume><issue>8</issue><spage>4748</spage><epage>4753</epage><pages>4748-4753</pages><issn>0027-8424</issn><eissn>1091-6490</eissn><abstract>The discovery of circulating fetal nucleic acid in maternal plasma has opened up new possibilities for noninvasive prenatal diagnosis. Thus far, a gender- and polymorphism-independent fetal-specific target that can be used for prenatal screening and monitoring in all pregnant women has not been reported. In addition, the origin of such circulating nucleic acid has remained unclear. Here we provide direct evidence that the placenta is an important source of fetal nucleic acid release into maternal plasma by demonstrating that mRNA transcripts from placenta-expressed genes are readily detectable in maternal plasma. The surprising stability of such placental mRNA species in maternal plasma and their rapid clearance after delivery demonstrate that such circulating mRNA molecules are practical markers for clinical use. The measurement of such plasma mRNA markers has provided a gender-independent approach for noninvasive prenatal gene expression profiling and has opened up numerous research and diagnostic possibilities.</abstract><cop>United States</cop><pub>National Academy of Sciences</pub><pmid>12644709</pmid><doi>10.1073/pnas.0637450100</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0027-8424 |
| ispartof | Proceedings of the National Academy of Sciences - PNAS, 2003-04, Vol.100 (8), p.4748-4753 |
| issn | 0027-8424 1091-6490 |
| language | eng |
| recordid | cdi_pnas_primary_100_8_4748 |
| source | MEDLINE; Jstor Complete Legacy; PubMed Central; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry |
| subjects | Base Sequence Biological Sciences Biological Transport, Active Blood Blood plasma DNA DNA Primers - genetics Female Fetus - metabolism Fetuses Gene Expression Profiling Gestational Age Humans Maternal-Fetal Exchange - genetics Medical research Messenger RNA Nucleic acids Placenta Placenta - metabolism Placental Lactogen - genetics Plasma Plasma stability Pregnancy Prenatal Diagnosis Reverse Transcriptase Polymerase Chain Reaction Ribonucleic acid RNA RNA Stability RNA, Messenger - blood RNA, Messenger - genetics RNA, Messenger - metabolism |
| title | mRNA of Placental Origin Is Readily Detectable in Maternal Plasma |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-20T13%3A08%3A20IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-jstor_pnas_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=mRNA%20of%20Placental%20Origin%20Is%20Readily%20Detectable%20in%20Maternal%20Plasma&rft.jtitle=Proceedings%20of%20the%20National%20Academy%20of%20Sciences%20-%20PNAS&rft.au=Enders%20K.%20O.%20Ng&rft.date=2003-04-15&rft.volume=100&rft.issue=8&rft.spage=4748&rft.epage=4753&rft.pages=4748-4753&rft.issn=0027-8424&rft.eissn=1091-6490&rft_id=info:doi/10.1073/pnas.0637450100&rft_dat=%3Cjstor_pnas_%3E3144013%3C/jstor_pnas_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=201375690&rft_id=info:pmid/12644709&rft_jstor_id=3144013&rfr_iscdi=true |