Enhanced biocontrol activity of Trichoderma through inactivation of a mitogen-activated protein kinase
The production of lytic enzymes in Trichoderma is considered determinant in its parasitic response against fungal species. A mitogen-activated protein kinase encoding gene, tvk1, from Trichoderma virens was cloned, and its role during the mycoparasitism, conidiation, and biocontrol was examined in t...
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| Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 2003-12, Vol.100 (26), p.15965-15970 |
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| container_title | Proceedings of the National Academy of Sciences - PNAS |
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| creator | Mendoza-Mendoza, A Pozo, M.J Grzegorski, D Martinez, P Garcia, J.M Olmedo-Monfil, V Cortes, C Kenerley, C Herrera-Estrella, A |
| description | The production of lytic enzymes in Trichoderma is considered determinant in its parasitic response against fungal species. A mitogen-activated protein kinase encoding gene, tvk1, from Trichoderma virens was cloned, and its role during the mycoparasitism, conidiation, and biocontrol was examined in tvk1 null mutants. These mutants showed a clear increase in the level of the expression of mycoparasitism-related genes under simulated mycoparasitism and during direct confrontation with the plant pathogen Rhizoctonia solani. The null mutants displayed an increased protein secretion phenotype as measured by the production of lytic enzymes in culture supernatant compared to the wild type. Consistently, biocontrol assays demonstrated that the null mutants were considerably more effective in disease control than the wild-type strain or a chemical fungicide. In addition, tvk1 gene disruptant strains sporulated abundantly in submerged cultures, a condition that is not conducive to sporulation in the wild type. These data suggest that Tvk1 acts as a negative modulator during host sensing and sporulation in T. virens. |
| doi_str_mv | 10.1073/pnas.2136716100 |
| format | Article |
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A mitogen-activated protein kinase encoding gene, tvk1, from Trichoderma virens was cloned, and its role during the mycoparasitism, conidiation, and biocontrol was examined in tvk1 null mutants. These mutants showed a clear increase in the level of the expression of mycoparasitism-related genes under simulated mycoparasitism and during direct confrontation with the plant pathogen Rhizoctonia solani. The null mutants displayed an increased protein secretion phenotype as measured by the production of lytic enzymes in culture supernatant compared to the wild type. Consistently, biocontrol assays demonstrated that the null mutants were considerably more effective in disease control than the wild-type strain or a chemical fungicide. In addition, tvk1 gene disruptant strains sporulated abundantly in submerged cultures, a condition that is not conducive to sporulation in the wild type. These data suggest that Tvk1 acts as a negative modulator during host sensing and sporulation in T. virens.</description><identifier>ISSN: 0027-8424</identifier><identifier>EISSN: 1091-6490</identifier><identifier>DOI: 10.1073/pnas.2136716100</identifier><identifier>PMID: 14673101</identifier><language>eng</language><publisher>United States: National Academy of Sciences</publisher><subject>biological control ; biological control agents ; Biological Sciences ; Carbon ; Cell walls ; Cloning, Molecular ; conidiation ; cotton ; Enzyme Inhibitors - pharmacology ; Enzymes ; fungal antagonists ; fungal diseases of plants ; Fungal spores ; Fungi ; gene activation ; Genes ; Genetic Vectors ; Gossypium - microbiology ; Gossypium hirsutum ; Hypocrea virens ; messenger RNA ; Microbiology ; Mitogen-Activated Protein Kinases - antagonists & inhibitors ; Mitogen-Activated Protein Kinases - genetics ; Molecular Sequence Data ; mutants ; mycoparasitism ; Nitrogen ; nucleotide sequences ; Parasite hosts ; Parasites ; parasitism ; Pest Control, Biological - methods ; plant pathogenic fungi ; Plant Roots - microbiology ; Plants ; Proteins ; Pythium ultimum ; Rhizoctonia solani ; seed inoculation ; sporulation ; structural genes ; Thanatephorus cucumeris ; Trichoderma ; Trichoderma - drug effects ; Trichoderma - genetics ; Trichoderma - pathogenicity ; tvk1 gene ; Tvk1 protein</subject><ispartof>Proceedings of the National Academy of Sciences - PNAS, 2003-12, Vol.100 (26), p.15965-15970</ispartof><rights>Copyright 1993-2003 National Academy of Sciences of the United States of America</rights><rights>Copyright National Academy of Sciences Dec 23, 2003</rights><rights>Copyright © 2003, The National Academy of Sciences 2003</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c550t-13db7a9b226215efaa728478547b41cf5c1002a3e431aca3358b0c48a8be19ba3</citedby><cites>FETCH-LOGICAL-c550t-13db7a9b226215efaa728478547b41cf5c1002a3e431aca3358b0c48a8be19ba3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.pnas.org/content/100/26.cover.gif</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/3149114$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/3149114$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,723,776,780,799,881,27901,27902,53766,53768,57992,58225</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/14673101$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Mendoza-Mendoza, A</creatorcontrib><creatorcontrib>Pozo, M.J</creatorcontrib><creatorcontrib>Grzegorski, D</creatorcontrib><creatorcontrib>Martinez, P</creatorcontrib><creatorcontrib>Garcia, J.M</creatorcontrib><creatorcontrib>Olmedo-Monfil, V</creatorcontrib><creatorcontrib>Cortes, C</creatorcontrib><creatorcontrib>Kenerley, C</creatorcontrib><creatorcontrib>Herrera-Estrella, A</creatorcontrib><title>Enhanced biocontrol activity of Trichoderma through inactivation of a mitogen-activated protein kinase</title><title>Proceedings of the National Academy of Sciences - PNAS</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>The production of lytic enzymes in Trichoderma is considered determinant in its parasitic response against fungal species. A mitogen-activated protein kinase encoding gene, tvk1, from Trichoderma virens was cloned, and its role during the mycoparasitism, conidiation, and biocontrol was examined in tvk1 null mutants. These mutants showed a clear increase in the level of the expression of mycoparasitism-related genes under simulated mycoparasitism and during direct confrontation with the plant pathogen Rhizoctonia solani. The null mutants displayed an increased protein secretion phenotype as measured by the production of lytic enzymes in culture supernatant compared to the wild type. Consistently, biocontrol assays demonstrated that the null mutants were considerably more effective in disease control than the wild-type strain or a chemical fungicide. In addition, tvk1 gene disruptant strains sporulated abundantly in submerged cultures, a condition that is not conducive to sporulation in the wild type. These data suggest that Tvk1 acts as a negative modulator during host sensing and sporulation in T. virens.</description><subject>biological control</subject><subject>biological control agents</subject><subject>Biological Sciences</subject><subject>Carbon</subject><subject>Cell walls</subject><subject>Cloning, Molecular</subject><subject>conidiation</subject><subject>cotton</subject><subject>Enzyme Inhibitors - pharmacology</subject><subject>Enzymes</subject><subject>fungal antagonists</subject><subject>fungal diseases of plants</subject><subject>Fungal spores</subject><subject>Fungi</subject><subject>gene activation</subject><subject>Genes</subject><subject>Genetic Vectors</subject><subject>Gossypium - microbiology</subject><subject>Gossypium hirsutum</subject><subject>Hypocrea virens</subject><subject>messenger RNA</subject><subject>Microbiology</subject><subject>Mitogen-Activated Protein Kinases - antagonists & inhibitors</subject><subject>Mitogen-Activated Protein Kinases - genetics</subject><subject>Molecular Sequence Data</subject><subject>mutants</subject><subject>mycoparasitism</subject><subject>Nitrogen</subject><subject>nucleotide sequences</subject><subject>Parasite hosts</subject><subject>Parasites</subject><subject>parasitism</subject><subject>Pest Control, Biological - methods</subject><subject>plant pathogenic fungi</subject><subject>Plant Roots - microbiology</subject><subject>Plants</subject><subject>Proteins</subject><subject>Pythium ultimum</subject><subject>Rhizoctonia solani</subject><subject>seed inoculation</subject><subject>sporulation</subject><subject>structural genes</subject><subject>Thanatephorus cucumeris</subject><subject>Trichoderma</subject><subject>Trichoderma - drug effects</subject><subject>Trichoderma - genetics</subject><subject>Trichoderma - pathogenicity</subject><subject>tvk1 gene</subject><subject>Tvk1 protein</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkTtvFDEUhUcIRJZATYNgRIFEMcm9fs4UFCgKDykSBUltebyeXS-z9mJ7IvLv8bCrLNCksuT7nWOfe6rqJcIZgqTnO6_TGUEqJAoEeFQtEDpsBOvgcbUAILJpGWEn1bOUNgDQ8RaeVifIhKQIuKiGS7_W3thl3btggs8xjLU22d26fFeHob6OzqzD0satrvM6hmm1rp3_Q-jsgp8ZXW9dDivrm8N9sdvFkK3z9Y8CJ_u8ejLoMdkXh_O0uvl0eX3xpbn69vnrxcerxnAOuUG67KXuekIEQW4HrSVpmWw5kz1DM3BTQhJNLaOojaaUtz0Y1uq2t9j1mp5WH_a-u6nf2qWxJZAe1S66rY53Kmin_p14t1arcKsoSCFF0b876GP4OdmU1dYlY8dRexumpCSyllPBHgSxI5xBJwv49j9wE6boyxIUAWRAWjo_e76HTAwpRTvc_xhBzUWruWh1LLooXv8d9Mgfmi1AfQBm5dEOFBEKeSd4Qd4_gKhhGsdsf-XCvtqzm5RDvIcpsg5xXseb_XjQQelVdEndfC_5KCAIpLyjvwH0_NEu</recordid><startdate>20031223</startdate><enddate>20031223</enddate><creator>Mendoza-Mendoza, A</creator><creator>Pozo, M.J</creator><creator>Grzegorski, D</creator><creator>Martinez, P</creator><creator>Garcia, J.M</creator><creator>Olmedo-Monfil, V</creator><creator>Cortes, C</creator><creator>Kenerley, C</creator><creator>Herrera-Estrella, A</creator><general>National Academy of Sciences</general><general>National Acad Sciences</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QG</scope><scope>7QL</scope><scope>7QP</scope><scope>7QR</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TK</scope><scope>7TM</scope><scope>7TO</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7T7</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20031223</creationdate><title>Enhanced biocontrol activity of Trichoderma through inactivation of a mitogen-activated protein kinase</title><author>Mendoza-Mendoza, A ; 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A mitogen-activated protein kinase encoding gene, tvk1, from Trichoderma virens was cloned, and its role during the mycoparasitism, conidiation, and biocontrol was examined in tvk1 null mutants. These mutants showed a clear increase in the level of the expression of mycoparasitism-related genes under simulated mycoparasitism and during direct confrontation with the plant pathogen Rhizoctonia solani. The null mutants displayed an increased protein secretion phenotype as measured by the production of lytic enzymes in culture supernatant compared to the wild type. Consistently, biocontrol assays demonstrated that the null mutants were considerably more effective in disease control than the wild-type strain or a chemical fungicide. In addition, tvk1 gene disruptant strains sporulated abundantly in submerged cultures, a condition that is not conducive to sporulation in the wild type. These data suggest that Tvk1 acts as a negative modulator during host sensing and sporulation in T. virens.</abstract><cop>United States</cop><pub>National Academy of Sciences</pub><pmid>14673101</pmid><doi>10.1073/pnas.2136716100</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | biological control biological control agents Biological Sciences Carbon Cell walls Cloning, Molecular conidiation cotton Enzyme Inhibitors - pharmacology Enzymes fungal antagonists fungal diseases of plants Fungal spores Fungi gene activation Genes Genetic Vectors Gossypium - microbiology Gossypium hirsutum Hypocrea virens messenger RNA Microbiology Mitogen-Activated Protein Kinases - antagonists & inhibitors Mitogen-Activated Protein Kinases - genetics Molecular Sequence Data mutants mycoparasitism Nitrogen nucleotide sequences Parasite hosts Parasites parasitism Pest Control, Biological - methods plant pathogenic fungi Plant Roots - microbiology Plants Proteins Pythium ultimum Rhizoctonia solani seed inoculation sporulation structural genes Thanatephorus cucumeris Trichoderma Trichoderma - drug effects Trichoderma - genetics Trichoderma - pathogenicity tvk1 gene Tvk1 protein |
| title | Enhanced biocontrol activity of Trichoderma through inactivation of a mitogen-activated protein kinase |
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