Early neutrophil activation and NETs release in the pristane-induced lupus mice model
NETosis is recognized as an important source of autoantigens. Therefore, we hypothesized whether the pristane-induced lupus mice model shows early activation of neutrophils, the presence of low-density granulocytes (LDGs), and neutrophil extracellular traps (NETs) release, which could contribute to...
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| creator | Carrasco, Solange Liphaus, Bernadete L Peixoto, Tatiana Vasconcelos Lima, Thais Martins Ariga, Sueli Kunimi Kubo Jesus Queiroz, Zelita Aparecida de Matos Lobo, Thays Catanozi, Sergio Rodrigues, Letícia Gomes Filho, Antônio Santos Teodoro, Walcy Rosolia Velosa, Ana Paula Pereira Levy, Débora Soriano, Francisco Garcia Goldenstein-Schainberg, Cláudia |
| description | NETosis is recognized as an important source of autoantigens. Therefore, we hypothesized whether the pristane-induced lupus mice model shows early activation of neutrophils, the presence of low-density granulocytes (LDGs), and neutrophil extracellular traps (NETs) release, which could contribute to the development of a lupus phenotype.
Twelve female wild-type Balb/c mice were intraperitoneally injected with pristane (n = 6; pristane group) or saline (n = 6; control group). Five days after the injection, blood, peritoneal lavage, bone marrow, and spleen samples were collected for flow cytometry analyses of activated neutrophils (Ly6G+CD11b+), LDGs (CD15+CD14low), and NETs release (Sytox Green+).
The pristane-induced mice group had a significantly increased number of blood activated neutrophils and LDGs as well as NETs released by these cells compared to the saline-injected control group and the basal values determined 12 days before the injection. The pristane group also had a significantly increased number of activated neutrophils, LDGs, and NETs released compared to the control group for the peritoneal lavage and bone marrow, except total cell count in spleen.
We demonstrated early changes in the innate immune response such as an increased number of activated neutrophils and LDGs and mainly increased NETosis in the pristane-induced mice model which may be considered as the primary event triggering lupus development. |
| doi_str_mv | 10.1371/journal.pone.0306943 |
| format | Article |
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Twelve female wild-type Balb/c mice were intraperitoneally injected with pristane (n = 6; pristane group) or saline (n = 6; control group). Five days after the injection, blood, peritoneal lavage, bone marrow, and spleen samples were collected for flow cytometry analyses of activated neutrophils (Ly6G+CD11b+), LDGs (CD15+CD14low), and NETs release (Sytox Green+).
The pristane-induced mice group had a significantly increased number of blood activated neutrophils and LDGs as well as NETs released by these cells compared to the saline-injected control group and the basal values determined 12 days before the injection. The pristane group also had a significantly increased number of activated neutrophils, LDGs, and NETs released compared to the control group for the peritoneal lavage and bone marrow, except total cell count in spleen.
We demonstrated early changes in the innate immune response such as an increased number of activated neutrophils and LDGs and mainly increased NETosis in the pristane-induced mice model which may be considered as the primary event triggering lupus development.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0306943</identifier><identifier>PMID: 39752468</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Animals ; Autoantigens ; Autoimmune diseases ; Blood ; Bone marrow ; CD11b antigen ; Cell activation ; Cells ; Cloning ; Disease Models, Animal ; Extracellular Traps - immunology ; Extracellular Traps - metabolism ; Female ; Flow cytometry ; Granulocytes ; Granulocytes - immunology ; Granulocytes - metabolism ; Health aspects ; Immune response ; Injection ; Innate immunity ; Laboratory animals ; Lavage ; Leukocytes (granulocytic) ; Leukocytes (neutrophilic) ; Lupus ; Lupus Erythematosus, Systemic - chemically induced ; Lupus Erythematosus, Systemic - immunology ; Lupus Erythematosus, Systemic - pathology ; Medical research ; Medicine, Experimental ; Mice ; Mice, Inbred BALB C ; Monoclonal antibodies ; Neutrophil Activation ; Neutrophils ; Neutrophils - immunology ; Peritoneum ; Phenotypes ; Physiological aspects ; Pristane ; Spleen ; Spleen - immunology ; Systemic lupus erythematosus ; Terpenes</subject><ispartof>PloS one, 2025, Vol.20 (1), p.e0306943</ispartof><rights>Copyright: © 2025 Carrasco et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.</rights><rights>COPYRIGHT 2025 Public Library of Science</rights><rights>2025 Carrasco et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2025 Carrasco et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c3243-76ad29f085ccbebf81817fe29c6abbce2238f36ad839be02821eb6d3dd666caa3</cites><orcidid>0000-0003-2469-9744 ; 0000-0003-4898-0135</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0306943&type=printable$$EPDF$$P50$$Gplos$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://journals.plos.org/plosone/article?id=10.1371/journal.pone.0306943$$EHTML$$P50$$Gplos$$Hfree_for_read</linktohtml><link.rule.ids>314,776,780,860,2096,2915,4010,23845,27900,27901,27902,79569,79570</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/39752468$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Carrasco, Solange</creatorcontrib><creatorcontrib>Liphaus, Bernadete L</creatorcontrib><creatorcontrib>Peixoto, Tatiana Vasconcelos</creatorcontrib><creatorcontrib>Lima, Thais Martins</creatorcontrib><creatorcontrib>Ariga, Sueli Kunimi Kubo</creatorcontrib><creatorcontrib>Jesus Queiroz, Zelita Aparecida</creatorcontrib><creatorcontrib>de Matos Lobo, Thays</creatorcontrib><creatorcontrib>Catanozi, Sergio</creatorcontrib><creatorcontrib>Rodrigues, Letícia Gomes</creatorcontrib><creatorcontrib>Filho, Antônio Santos</creatorcontrib><creatorcontrib>Teodoro, Walcy Rosolia</creatorcontrib><creatorcontrib>Velosa, Ana Paula Pereira</creatorcontrib><creatorcontrib>Levy, Débora</creatorcontrib><creatorcontrib>Soriano, Francisco Garcia</creatorcontrib><creatorcontrib>Goldenstein-Schainberg, Cláudia</creatorcontrib><title>Early neutrophil activation and NETs release in the pristane-induced lupus mice model</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>NETosis is recognized as an important source of autoantigens. Therefore, we hypothesized whether the pristane-induced lupus mice model shows early activation of neutrophils, the presence of low-density granulocytes (LDGs), and neutrophil extracellular traps (NETs) release, which could contribute to the development of a lupus phenotype.
Twelve female wild-type Balb/c mice were intraperitoneally injected with pristane (n = 6; pristane group) or saline (n = 6; control group). Five days after the injection, blood, peritoneal lavage, bone marrow, and spleen samples were collected for flow cytometry analyses of activated neutrophils (Ly6G+CD11b+), LDGs (CD15+CD14low), and NETs release (Sytox Green+).
The pristane-induced mice group had a significantly increased number of blood activated neutrophils and LDGs as well as NETs released by these cells compared to the saline-injected control group and the basal values determined 12 days before the injection. The pristane group also had a significantly increased number of activated neutrophils, LDGs, and NETs released compared to the control group for the peritoneal lavage and bone marrow, except total cell count in spleen.
We demonstrated early changes in the innate immune response such as an increased number of activated neutrophils and LDGs and mainly increased NETosis in the pristane-induced mice model which may be considered as the primary event triggering lupus development.</description><subject>Animals</subject><subject>Autoantigens</subject><subject>Autoimmune diseases</subject><subject>Blood</subject><subject>Bone marrow</subject><subject>CD11b antigen</subject><subject>Cell activation</subject><subject>Cells</subject><subject>Cloning</subject><subject>Disease Models, Animal</subject><subject>Extracellular Traps - immunology</subject><subject>Extracellular Traps - metabolism</subject><subject>Female</subject><subject>Flow cytometry</subject><subject>Granulocytes</subject><subject>Granulocytes - immunology</subject><subject>Granulocytes - metabolism</subject><subject>Health aspects</subject><subject>Immune response</subject><subject>Injection</subject><subject>Innate immunity</subject><subject>Laboratory animals</subject><subject>Lavage</subject><subject>Leukocytes (granulocytic)</subject><subject>Leukocytes (neutrophilic)</subject><subject>Lupus</subject><subject>Lupus Erythematosus, Systemic - chemically induced</subject><subject>Lupus Erythematosus, Systemic - immunology</subject><subject>Lupus Erythematosus, Systemic - pathology</subject><subject>Medical research</subject><subject>Medicine, Experimental</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Monoclonal antibodies</subject><subject>Neutrophil Activation</subject><subject>Neutrophils</subject><subject>Neutrophils - immunology</subject><subject>Peritoneum</subject><subject>Phenotypes</subject><subject>Physiological aspects</subject><subject>Pristane</subject><subject>Spleen</subject><subject>Spleen - immunology</subject><subject>Systemic lupus 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neutrophil activation and NETs release in the pristane-induced lupus mice model</title><author>Carrasco, Solange ; Liphaus, Bernadete L ; Peixoto, Tatiana Vasconcelos ; Lima, Thais Martins ; Ariga, Sueli Kunimi Kubo ; Jesus Queiroz, Zelita Aparecida ; de Matos Lobo, Thays ; Catanozi, Sergio ; Rodrigues, Letícia Gomes ; Filho, Antônio Santos ; Teodoro, Walcy Rosolia ; Velosa, Ana Paula Pereira ; Levy, Débora ; Soriano, Francisco Garcia ; Goldenstein-Schainberg, Cláudia</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3243-76ad29f085ccbebf81817fe29c6abbce2238f36ad839be02821eb6d3dd666caa3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2025</creationdate><topic>Animals</topic><topic>Autoantigens</topic><topic>Autoimmune diseases</topic><topic>Blood</topic><topic>Bone marrow</topic><topic>CD11b antigen</topic><topic>Cell activation</topic><topic>Cells</topic><topic>Cloning</topic><topic>Disease 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immunology</topic><topic>Peritoneum</topic><topic>Phenotypes</topic><topic>Physiological aspects</topic><topic>Pristane</topic><topic>Spleen</topic><topic>Spleen - immunology</topic><topic>Systemic lupus erythematosus</topic><topic>Terpenes</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Carrasco, Solange</creatorcontrib><creatorcontrib>Liphaus, Bernadete L</creatorcontrib><creatorcontrib>Peixoto, Tatiana Vasconcelos</creatorcontrib><creatorcontrib>Lima, Thais Martins</creatorcontrib><creatorcontrib>Ariga, Sueli Kunimi Kubo</creatorcontrib><creatorcontrib>Jesus Queiroz, Zelita Aparecida</creatorcontrib><creatorcontrib>de Matos Lobo, Thays</creatorcontrib><creatorcontrib>Catanozi, Sergio</creatorcontrib><creatorcontrib>Rodrigues, Letícia Gomes</creatorcontrib><creatorcontrib>Filho, Antônio Santos</creatorcontrib><creatorcontrib>Teodoro, Walcy Rosolia</creatorcontrib><creatorcontrib>Velosa, Ana Paula 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Academic</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Carrasco, Solange</au><au>Liphaus, Bernadete L</au><au>Peixoto, Tatiana Vasconcelos</au><au>Lima, Thais Martins</au><au>Ariga, Sueli Kunimi Kubo</au><au>Jesus Queiroz, Zelita Aparecida</au><au>de Matos Lobo, Thays</au><au>Catanozi, Sergio</au><au>Rodrigues, Letícia Gomes</au><au>Filho, Antônio Santos</au><au>Teodoro, Walcy Rosolia</au><au>Velosa, Ana Paula Pereira</au><au>Levy, Débora</au><au>Soriano, Francisco Garcia</au><au>Goldenstein-Schainberg, Cláudia</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Early neutrophil activation and NETs release in the pristane-induced lupus mice model</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2025</date><risdate>2025</risdate><volume>20</volume><issue>1</issue><spage>e0306943</spage><pages>e0306943-</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>NETosis is recognized as an important source of autoantigens. Therefore, we hypothesized whether the pristane-induced lupus mice model shows early activation of neutrophils, the presence of low-density granulocytes (LDGs), and neutrophil extracellular traps (NETs) release, which could contribute to the development of a lupus phenotype.
Twelve female wild-type Balb/c mice were intraperitoneally injected with pristane (n = 6; pristane group) or saline (n = 6; control group). Five days after the injection, blood, peritoneal lavage, bone marrow, and spleen samples were collected for flow cytometry analyses of activated neutrophils (Ly6G+CD11b+), LDGs (CD15+CD14low), and NETs release (Sytox Green+).
The pristane-induced mice group had a significantly increased number of blood activated neutrophils and LDGs as well as NETs released by these cells compared to the saline-injected control group and the basal values determined 12 days before the injection. The pristane group also had a significantly increased number of activated neutrophils, LDGs, and NETs released compared to the control group for the peritoneal lavage and bone marrow, except total cell count in spleen.
We demonstrated early changes in the innate immune response such as an increased number of activated neutrophils and LDGs and mainly increased NETosis in the pristane-induced mice model which may be considered as the primary event triggering lupus development.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>39752468</pmid><doi>10.1371/journal.pone.0306943</doi><orcidid>https://orcid.org/0000-0003-2469-9744</orcidid><orcidid>https://orcid.org/0000-0003-4898-0135</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2025, Vol.20 (1), p.e0306943 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_3151369547 |
| source | Public Library of Science (PLoS) Journals Open Access; MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Animals Autoantigens Autoimmune diseases Blood Bone marrow CD11b antigen Cell activation Cells Cloning Disease Models, Animal Extracellular Traps - immunology Extracellular Traps - metabolism Female Flow cytometry Granulocytes Granulocytes - immunology Granulocytes - metabolism Health aspects Immune response Injection Innate immunity Laboratory animals Lavage Leukocytes (granulocytic) Leukocytes (neutrophilic) Lupus Lupus Erythematosus, Systemic - chemically induced Lupus Erythematosus, Systemic - immunology Lupus Erythematosus, Systemic - pathology Medical research Medicine, Experimental Mice Mice, Inbred BALB C Monoclonal antibodies Neutrophil Activation Neutrophils Neutrophils - immunology Peritoneum Phenotypes Physiological aspects Pristane Spleen Spleen - immunology Systemic lupus erythematosus Terpenes |
| title | Early neutrophil activation and NETs release in the pristane-induced lupus mice model |
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