Activity of glucose-6-phosphate dehydrogenease and its correlation with inflammatory factors in diabetic retinopathy
This study aims to explore glucose-6-phosphate dehydrogenase (G6PD) activity in diabetic retinopathy (DR) and its correlation with inflammatory factors, elucidating the regulatory role of G6PD in DR pathology. A total of 151 T2DM patients were divided into three groups: diabetes without retinopathy...
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| Veröffentlicht in: | PloS one 2024-12, Vol.19 (12), p.e0312452 |
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| creator | Liu, Dan Cheng, Chuchu Zhou, Lan Zeng, Qiqiao Zi, Tao Chen, Gongyi Sun, Hongyan Li, Cunzi Wang, Jun Yang, Ming-Ming |
| description | This study aims to explore glucose-6-phosphate dehydrogenase (G6PD) activity in diabetic retinopathy (DR) and its correlation with inflammatory factors, elucidating the regulatory role of G6PD in DR pathology.
A total of 151 T2DM patients were divided into three groups: diabetes without retinopathy (DNR, n = 59), non-proliferative retinopathy (NPDR, n = 46) and proliferative retinopathy (PDR, n = 49). Plasma G6PD activity was measured by a Randox G6PD kit and compared between these groups. Then the G6PD activity was correlated with inflammatory cytokines and metabolic parameters in these patients. A STZ-induced diabetic rat model was established, G6PD activity was validated by western blot and immunofluorescence staining in the retina of this model.
Plasma G6PD activity decreased in the order of DNR, NPDR and PDR groups (P |
| doi_str_mv | 10.1371/journal.pone.0312452 |
| format | Article |
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A total of 151 T2DM patients were divided into three groups: diabetes without retinopathy (DNR, n = 59), non-proliferative retinopathy (NPDR, n = 46) and proliferative retinopathy (PDR, n = 49). Plasma G6PD activity was measured by a Randox G6PD kit and compared between these groups. Then the G6PD activity was correlated with inflammatory cytokines and metabolic parameters in these patients. A STZ-induced diabetic rat model was established, G6PD activity was validated by western blot and immunofluorescence staining in the retina of this model.
Plasma G6PD activity decreased in the order of DNR, NPDR and PDR groups (P<0.01). G6PD activity was negatively correlated with IL-6, IL-8, TNF-α, cholesterol, and LDL (r = -0.1625, -0.1808, -0.1865, -0.1747, r = -0.1807, P<0.05). Multiple regression analysis showed TNF-α, IL-6, and LDL were independent related factors for G6PD. Logistic regression analysis showed G6PD, triglyceride, cholesterol, IL-8, TNF-α, and macular edema were influencing factors for T2DM with DR. Western Blot analysis indicated a significant reduction of G6PD expression in the retina, and immunofluorescence staining showed distribution of G6PD especially in the retinal endothelium cell decreased.
G6PD may play an important role in DR occurrence and progression, with decreased expression correlating closely with lipid metabolism and inflammatory factors.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0312452</identifier><identifier>PMID: 39621725</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Aged ; Animals ; Antibodies ; Biology and Life Sciences ; Blood pressure ; Cardiovascular disease ; Care and treatment ; Cholesterol ; Correlation ; Creatinine ; Cytokines ; Cytokines - blood ; Cytokines - metabolism ; Dehydrogenases ; Development and progression ; Dextrose ; Diabetes ; Diabetes mellitus ; Diabetes Mellitus, Experimental - complications ; Diabetes Mellitus, Experimental - metabolism ; Diabetes Mellitus, Type 2 - complications ; Diabetes Mellitus, Type 2 - metabolism ; Diabetic retinopathy ; Diabetic Retinopathy - blood ; Diabetic Retinopathy - metabolism ; Diabetic Retinopathy - pathology ; Diagnosis ; Edema ; Endothelium ; Ethics ; Experiments ; Female ; Glucose ; Glucose 6 phosphate dehydrogenase ; Glucosephosphate dehydrogenase ; Glucosephosphate Dehydrogenase - metabolism ; Health aspects ; Hemoglobin ; Humans ; Immunofluorescence ; Inflammation ; Interleukin 6 ; Interleukin 8 ; Interleukin-6 - blood ; Interleukin-6 - metabolism ; Laboratory animals ; Lipid metabolism ; Lipids ; Lipoproteins ; Low density lipoprotein ; Low density lipoproteins ; Male ; Mediation ; Medicine and Health Sciences ; Middle Aged ; Multiple regression analysis ; Oxidative stress ; Patients ; Phosphates ; Physical Sciences ; Proteins ; Rats ; Rats, Sprague-Dawley ; Regression analysis ; Research and Analysis Methods ; Retina ; Retina - metabolism ; Retina - pathology ; Retinopathy ; Review boards ; Staining ; Triglycerides ; Tumor Necrosis Factor-alpha - blood ; Tumor Necrosis Factor-alpha - metabolism ; Tumor necrosis factor-TNF ; Tumor necrosis factor-α ; Type 2 diabetes ; Variance analysis</subject><ispartof>PloS one, 2024-12, Vol.19 (12), p.e0312452</ispartof><rights>Copyright: © 2024 Liu et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.</rights><rights>COPYRIGHT 2024 Public Library of Science</rights><rights>2024 Liu et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2024 Liu et al 2024 Liu et al</rights><rights>2024 Liu et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c572t-d10a6111ac96f138abb9057382ed845bafa780d1069551dbdfa458eb323aed193</cites><orcidid>0009-0002-2738-5679</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC11611225/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC11611225/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,2096,2915,23845,27901,27902,53766,53768,79342,79343</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/39621725$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Liu, Dan</creatorcontrib><creatorcontrib>Cheng, Chuchu</creatorcontrib><creatorcontrib>Zhou, Lan</creatorcontrib><creatorcontrib>Zeng, Qiqiao</creatorcontrib><creatorcontrib>Zi, Tao</creatorcontrib><creatorcontrib>Chen, Gongyi</creatorcontrib><creatorcontrib>Sun, Hongyan</creatorcontrib><creatorcontrib>Li, Cunzi</creatorcontrib><creatorcontrib>Wang, Jun</creatorcontrib><creatorcontrib>Yang, Ming-Ming</creatorcontrib><title>Activity of glucose-6-phosphate dehydrogenease and its correlation with inflammatory factors in diabetic retinopathy</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>This study aims to explore glucose-6-phosphate dehydrogenase (G6PD) activity in diabetic retinopathy (DR) and its correlation with inflammatory factors, elucidating the regulatory role of G6PD in DR pathology.
A total of 151 T2DM patients were divided into three groups: diabetes without retinopathy (DNR, n = 59), non-proliferative retinopathy (NPDR, n = 46) and proliferative retinopathy (PDR, n = 49). Plasma G6PD activity was measured by a Randox G6PD kit and compared between these groups. Then the G6PD activity was correlated with inflammatory cytokines and metabolic parameters in these patients. A STZ-induced diabetic rat model was established, G6PD activity was validated by western blot and immunofluorescence staining in the retina of this model.
Plasma G6PD activity decreased in the order of DNR, NPDR and PDR groups (P<0.01). G6PD activity was negatively correlated with IL-6, IL-8, TNF-α, cholesterol, and LDL (r = -0.1625, -0.1808, -0.1865, -0.1747, r = -0.1807, P<0.05). Multiple regression analysis showed TNF-α, IL-6, and LDL were independent related factors for G6PD. Logistic regression analysis showed G6PD, triglyceride, cholesterol, IL-8, TNF-α, and macular edema were influencing factors for T2DM with DR. Western Blot analysis indicated a significant reduction of G6PD expression in the retina, and immunofluorescence staining showed distribution of G6PD especially in the retinal endothelium cell decreased.
G6PD may play an important role in DR occurrence and progression, with decreased expression correlating closely with lipid metabolism and inflammatory factors.</description><subject>Aged</subject><subject>Animals</subject><subject>Antibodies</subject><subject>Biology and Life Sciences</subject><subject>Blood pressure</subject><subject>Cardiovascular disease</subject><subject>Care and treatment</subject><subject>Cholesterol</subject><subject>Correlation</subject><subject>Creatinine</subject><subject>Cytokines</subject><subject>Cytokines - blood</subject><subject>Cytokines - metabolism</subject><subject>Dehydrogenases</subject><subject>Development and progression</subject><subject>Dextrose</subject><subject>Diabetes</subject><subject>Diabetes mellitus</subject><subject>Diabetes Mellitus, Experimental - complications</subject><subject>Diabetes Mellitus, Experimental - metabolism</subject><subject>Diabetes Mellitus, Type 2 - complications</subject><subject>Diabetes Mellitus, Type 2 - metabolism</subject><subject>Diabetic retinopathy</subject><subject>Diabetic Retinopathy - blood</subject><subject>Diabetic Retinopathy - metabolism</subject><subject>Diabetic Retinopathy - pathology</subject><subject>Diagnosis</subject><subject>Edema</subject><subject>Endothelium</subject><subject>Ethics</subject><subject>Experiments</subject><subject>Female</subject><subject>Glucose</subject><subject>Glucose 6 phosphate dehydrogenase</subject><subject>Glucosephosphate dehydrogenase</subject><subject>Glucosephosphate Dehydrogenase - metabolism</subject><subject>Health aspects</subject><subject>Hemoglobin</subject><subject>Humans</subject><subject>Immunofluorescence</subject><subject>Inflammation</subject><subject>Interleukin 6</subject><subject>Interleukin 8</subject><subject>Interleukin-6 - blood</subject><subject>Interleukin-6 - metabolism</subject><subject>Laboratory animals</subject><subject>Lipid metabolism</subject><subject>Lipids</subject><subject>Lipoproteins</subject><subject>Low density lipoprotein</subject><subject>Low density lipoproteins</subject><subject>Male</subject><subject>Mediation</subject><subject>Medicine and Health Sciences</subject><subject>Middle Aged</subject><subject>Multiple regression analysis</subject><subject>Oxidative stress</subject><subject>Patients</subject><subject>Phosphates</subject><subject>Physical Sciences</subject><subject>Proteins</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Regression analysis</subject><subject>Research and Analysis Methods</subject><subject>Retina</subject><subject>Retina - metabolism</subject><subject>Retina - pathology</subject><subject>Retinopathy</subject><subject>Review boards</subject><subject>Staining</subject><subject>Triglycerides</subject><subject>Tumor Necrosis Factor-alpha - blood</subject><subject>Tumor Necrosis Factor-alpha - metabolism</subject><subject>Tumor necrosis factor-TNF</subject><subject>Tumor necrosis factor-α</subject><subject>Type 2 diabetes</subject><subject>Variance analysis</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><sourceid>DOA</sourceid><recordid>eNqNk1uL1TAQx4so7rr6DUQLguhDj03SS_okh8XLgYUFb69h2iRtDmlTk3T1fHtTT3c5lX2QQBImv_lnZpKJouco3SBSond7M9kB9GY0g9ikBOEsxw-ic1QRnBQ4JQ9P9mfRE-f2aZoTWhSPozNSFRiVOD-P_Lbx6kb5Q2xk3OqpMU4kRTJ2xo0deBFz0R24Na0YBDgRw8Bj5V3cGGuFBq_MEP9SvovVIDX0PXhjD7GEJqwuGGOuoBZeNbEN82BG8N3hafRIgnbi2bJeRN8_fvh2-Tm5uv60u9xeJU1eYp9wlEKBEIKmKiQiFOq6SvOSUCw4zfIaJJQ0DVRR5TniNZeQ5VTUBBMQPCR_Eb086o7aOLYUzDGCSB50AhCI3ZHgBvZstKoHe2AGFPtrMLZlYEP0WrCqRFRKwitMq4ygjCKa1VmZVmVVy7pEQev9cttU94I3YvAW9Ep0fTKojrXmhiEUssQ4DwpvFgVrfk7CedYr1witYRBmmgPP0goTXMzoq3_Q-9NbqBZCBuGJTLi4mUXZNsRfUITpTG3uocLgoldN-F5SBfvK4e3KITBe_PYtTM6x3dcv_89e_1izr0_YToD2nTN6mn-ZW4PZEWyscc4KeVdllLK5O26rwebuYEt3BLcXpy9053TbDuQPJxMK8Q</recordid><startdate>20241202</startdate><enddate>20241202</enddate><creator>Liu, Dan</creator><creator>Cheng, Chuchu</creator><creator>Zhou, Lan</creator><creator>Zeng, Qiqiao</creator><creator>Zi, Tao</creator><creator>Chen, Gongyi</creator><creator>Sun, Hongyan</creator><creator>Li, Cunzi</creator><creator>Wang, Jun</creator><creator>Yang, Ming-Ming</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope><orcidid>https://orcid.org/0009-0002-2738-5679</orcidid></search><sort><creationdate>20241202</creationdate><title>Activity of glucose-6-phosphate dehydrogenease and its correlation with inflammatory factors in diabetic retinopathy</title><author>Liu, Dan ; Cheng, Chuchu ; Zhou, Lan ; Zeng, Qiqiao ; Zi, Tao ; Chen, Gongyi ; Sun, Hongyan ; Li, Cunzi ; Wang, Jun ; Yang, Ming-Ming</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c572t-d10a6111ac96f138abb9057382ed845bafa780d1069551dbdfa458eb323aed193</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Aged</topic><topic>Animals</topic><topic>Antibodies</topic><topic>Biology and Life Sciences</topic><topic>Blood pressure</topic><topic>Cardiovascular disease</topic><topic>Care and treatment</topic><topic>Cholesterol</topic><topic>Correlation</topic><topic>Creatinine</topic><topic>Cytokines</topic><topic>Cytokines - blood</topic><topic>Cytokines - metabolism</topic><topic>Dehydrogenases</topic><topic>Development and progression</topic><topic>Dextrose</topic><topic>Diabetes</topic><topic>Diabetes mellitus</topic><topic>Diabetes Mellitus, Experimental - complications</topic><topic>Diabetes Mellitus, Experimental - metabolism</topic><topic>Diabetes Mellitus, Type 2 - complications</topic><topic>Diabetes Mellitus, Type 2 - metabolism</topic><topic>Diabetic retinopathy</topic><topic>Diabetic Retinopathy - blood</topic><topic>Diabetic Retinopathy - metabolism</topic><topic>Diabetic Retinopathy - pathology</topic><topic>Diagnosis</topic><topic>Edema</topic><topic>Endothelium</topic><topic>Ethics</topic><topic>Experiments</topic><topic>Female</topic><topic>Glucose</topic><topic>Glucose 6 phosphate dehydrogenase</topic><topic>Glucosephosphate dehydrogenase</topic><topic>Glucosephosphate Dehydrogenase - metabolism</topic><topic>Health aspects</topic><topic>Hemoglobin</topic><topic>Humans</topic><topic>Immunofluorescence</topic><topic>Inflammation</topic><topic>Interleukin 6</topic><topic>Interleukin 8</topic><topic>Interleukin-6 - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Liu, Dan</au><au>Cheng, Chuchu</au><au>Zhou, Lan</au><au>Zeng, Qiqiao</au><au>Zi, Tao</au><au>Chen, Gongyi</au><au>Sun, Hongyan</au><au>Li, Cunzi</au><au>Wang, Jun</au><au>Yang, Ming-Ming</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Activity of glucose-6-phosphate dehydrogenease and its correlation with inflammatory factors in diabetic retinopathy</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2024-12-02</date><risdate>2024</risdate><volume>19</volume><issue>12</issue><spage>e0312452</spage><pages>e0312452-</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>This study aims to explore glucose-6-phosphate dehydrogenase (G6PD) activity in diabetic retinopathy (DR) and its correlation with inflammatory factors, elucidating the regulatory role of G6PD in DR pathology.
A total of 151 T2DM patients were divided into three groups: diabetes without retinopathy (DNR, n = 59), non-proliferative retinopathy (NPDR, n = 46) and proliferative retinopathy (PDR, n = 49). Plasma G6PD activity was measured by a Randox G6PD kit and compared between these groups. Then the G6PD activity was correlated with inflammatory cytokines and metabolic parameters in these patients. A STZ-induced diabetic rat model was established, G6PD activity was validated by western blot and immunofluorescence staining in the retina of this model.
Plasma G6PD activity decreased in the order of DNR, NPDR and PDR groups (P<0.01). G6PD activity was negatively correlated with IL-6, IL-8, TNF-α, cholesterol, and LDL (r = -0.1625, -0.1808, -0.1865, -0.1747, r = -0.1807, P<0.05). Multiple regression analysis showed TNF-α, IL-6, and LDL were independent related factors for G6PD. Logistic regression analysis showed G6PD, triglyceride, cholesterol, IL-8, TNF-α, and macular edema were influencing factors for T2DM with DR. Western Blot analysis indicated a significant reduction of G6PD expression in the retina, and immunofluorescence staining showed distribution of G6PD especially in the retinal endothelium cell decreased.
G6PD may play an important role in DR occurrence and progression, with decreased expression correlating closely with lipid metabolism and inflammatory factors.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>39621725</pmid><doi>10.1371/journal.pone.0312452</doi><tpages>e0312452</tpages><orcidid>https://orcid.org/0009-0002-2738-5679</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2024-12, Vol.19 (12), p.e0312452 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_3135057193 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; Free Full-Text Journals in Chemistry; Public Library of Science (PLoS) |
| subjects | Aged Animals Antibodies Biology and Life Sciences Blood pressure Cardiovascular disease Care and treatment Cholesterol Correlation Creatinine Cytokines Cytokines - blood Cytokines - metabolism Dehydrogenases Development and progression Dextrose Diabetes Diabetes mellitus Diabetes Mellitus, Experimental - complications Diabetes Mellitus, Experimental - metabolism Diabetes Mellitus, Type 2 - complications Diabetes Mellitus, Type 2 - metabolism Diabetic retinopathy Diabetic Retinopathy - blood Diabetic Retinopathy - metabolism Diabetic Retinopathy - pathology Diagnosis Edema Endothelium Ethics Experiments Female Glucose Glucose 6 phosphate dehydrogenase Glucosephosphate dehydrogenase Glucosephosphate Dehydrogenase - metabolism Health aspects Hemoglobin Humans Immunofluorescence Inflammation Interleukin 6 Interleukin 8 Interleukin-6 - blood Interleukin-6 - metabolism Laboratory animals Lipid metabolism Lipids Lipoproteins Low density lipoprotein Low density lipoproteins Male Mediation Medicine and Health Sciences Middle Aged Multiple regression analysis Oxidative stress Patients Phosphates Physical Sciences Proteins Rats Rats, Sprague-Dawley Regression analysis Research and Analysis Methods Retina Retina - metabolism Retina - pathology Retinopathy Review boards Staining Triglycerides Tumor Necrosis Factor-alpha - blood Tumor Necrosis Factor-alpha - metabolism Tumor necrosis factor-TNF Tumor necrosis factor-α Type 2 diabetes Variance analysis |
| title | Activity of glucose-6-phosphate dehydrogenease and its correlation with inflammatory factors in diabetic retinopathy |
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