GC-MS metabolite profiling of Pseudocercospora fijiensis isolates resistant to thiabendazole
Black Sigatoka is the most widespread banana disease worldwide. It is caused by Pseudocercospora fijiensis, a fungal pathogen known for developing resistance to fungicides such as thiabendazole. Despite the increasing costs associated with the use of chemicals to control this disease, the pathogen&#...
Gespeichert in:
| Veröffentlicht in: | PloS one 2024-11, Vol.19 (11), p.e0313915 |
|---|---|
| Hauptverfasser: | , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | |
|---|---|
| container_issue | 11 |
| container_start_page | e0313915 |
| container_title | PloS one |
| container_volume | 19 |
| creator | Maridueña-Zavala, María Gabriela Chong-Aguirre, Pablo Antonio Freire-Peñaherrera, Andrea Moreno, Arturo Reyes-De-Corcuera, José Ignacio Jiménez-Feijoo, María Isabel Cevallos-Cevallos, Juan Manuel |
| description | Black Sigatoka is the most widespread banana disease worldwide. It is caused by Pseudocercospora fijiensis, a fungal pathogen known for developing resistance to fungicides such as thiabendazole. Despite the increasing costs associated with the use of chemicals to control this disease, the pathogen's mechanisms for fungicide resistance are not fully understood. The metabolite profiles of P. fijiensis isolates with different levels of resistance to thiabendazole were characterized by GC-MS. A total of 33 isolates were obtained from symptomatic banana plants and the sensitivity of each isolate to thiabendazole was assessed at 0, 1, 10, 100, 1000, and 10000 μg.mL-1. Then, the metabolite profile of each isolate was assessed using GC-MS. Metabolites such as hexadecanoic acid, tetradecanoic acid, octadecadienoic acid and octadecanoic acid were significantly over-accumulated in the presence of thiabendazole at 10 μg.mL-1. Phosphoric acid, L-proline, and D-allose increased in concentration with time in the presence of 100 μg.mL-1 of thiabendazole, and mannonic acid, 1-hexadecanol, D-sorbitol and tetracosanoic acid were only detected in the presence of the fungicide. Metabolic pathways including that of fructose, mannose metabolism, the biosynthesis of unsaturated fatty acids, and ABC transporters were upregulated in resistant isolates. Our findings show an increment of tetracosanoic (myristic) acid suggesting a possible β-tubulin-compensation mechanism in resistant isolates. The presence of myristic acid promoted the generation of diacylglycerol kinase δ which facilitated the production of β-tubulin in other studies. Additionally, important changes in the metabolite profiles were observed as soon as six hours after exposure to the fungicide showing an early response of the pathogen. To the best of our knowledge, this is the first report that describes the changes in the metabolite profile of P. fijiensis resistant to thiabendazole when exposed to the fungicide. |
| doi_str_mv | 10.1371/journal.pone.0313915 |
| format | Article |
| fullrecord | <record><control><sourceid>gale_plos_</sourceid><recordid>TN_cdi_plos_journals_3131776960</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A817044326</galeid><doaj_id>oai_doaj_org_article_88eed5e3c05b4b338a2b3da053fa8674</doaj_id><sourcerecordid>A817044326</sourcerecordid><originalsourceid>FETCH-LOGICAL-c4875-1c04644d57c3b60780a64b743fa0a9cc5ce0a9f381e3c8b9a20528f03bf43243</originalsourceid><addsrcrecordid>eNqNk1Fv0zAQxyMEYmPwDRBEQkLw0GLHTuI8oamCUWloiE08IVkX59y6cuPOdhDw6XFpNi1oDygPju3f_c_--y7LnlMyp6ym7zZu8D3Y-c71OCeMsoaWD7Jj2rBiVhWEPbzzf5Q9CWFDSMlEVT3OjlhT1kQU1XH2_Wwx-3yZbzFC66yJmO-808aafpU7nX8JOHROoVcu7JyHXJuNwT6YkJvgLEQMucc0jdDHPLo8rg202Hfw21l8mj3SYAM-G8eT7Orjh6vFp9n5xdlycXo-U1zU5YwqwivOu7JWrK1ILQhUvK0500CgUapUmEbNBEWmRNtAQcpCaMJazVnB2Un28iC7sy7I0ZcgkyW0rqumIolYHojOwUbuvNmC_yUdGPl3wfmVBB-NsiiFQOzKlIiULW8ZE1C0rINknQZR1fts78dsQ7vFTmEfPdiJ6HSnN2u5cj8kpaWgRSOSwptRwbvrAUOUWxMUWgs9uuFwcMEFY1VCX_2D3n-9kVpBuoHptUuJ1V5UngpaE55s2mvN76HS1-HWqFRF6dlxGvB2EpCYiD_jCoYQ5PLy6_-zF9-m7Os77BrBxnUqpiEa14cpyA-g8i4Ej_rWZUrkvglu3JD7JpBjE6SwF3df6DbopurZH9svAZc</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>3131776960</pqid></control><display><type>article</type><title>GC-MS metabolite profiling of Pseudocercospora fijiensis isolates resistant to thiabendazole</title><source>MEDLINE</source><source>DOAJ Directory of Open Access Journals</source><source>Public Library of Science (PLoS) Journals Open Access</source><source>EZB-FREE-00999 freely available EZB journals</source><source>PubMed Central</source><source>Free Full-Text Journals in Chemistry</source><creator>Maridueña-Zavala, María Gabriela ; Chong-Aguirre, Pablo Antonio ; Freire-Peñaherrera, Andrea ; Moreno, Arturo ; Reyes-De-Corcuera, José Ignacio ; Jiménez-Feijoo, María Isabel ; Cevallos-Cevallos, Juan Manuel</creator><contributor>Kniemeyer, Olaf</contributor><creatorcontrib>Maridueña-Zavala, María Gabriela ; Chong-Aguirre, Pablo Antonio ; Freire-Peñaherrera, Andrea ; Moreno, Arturo ; Reyes-De-Corcuera, José Ignacio ; Jiménez-Feijoo, María Isabel ; Cevallos-Cevallos, Juan Manuel ; Kniemeyer, Olaf</creatorcontrib><description>Black Sigatoka is the most widespread banana disease worldwide. It is caused by Pseudocercospora fijiensis, a fungal pathogen known for developing resistance to fungicides such as thiabendazole. Despite the increasing costs associated with the use of chemicals to control this disease, the pathogen's mechanisms for fungicide resistance are not fully understood. The metabolite profiles of P. fijiensis isolates with different levels of resistance to thiabendazole were characterized by GC-MS. A total of 33 isolates were obtained from symptomatic banana plants and the sensitivity of each isolate to thiabendazole was assessed at 0, 1, 10, 100, 1000, and 10000 μg.mL-1. Then, the metabolite profile of each isolate was assessed using GC-MS. Metabolites such as hexadecanoic acid, tetradecanoic acid, octadecadienoic acid and octadecanoic acid were significantly over-accumulated in the presence of thiabendazole at 10 μg.mL-1. Phosphoric acid, L-proline, and D-allose increased in concentration with time in the presence of 100 μg.mL-1 of thiabendazole, and mannonic acid, 1-hexadecanol, D-sorbitol and tetracosanoic acid were only detected in the presence of the fungicide. Metabolic pathways including that of fructose, mannose metabolism, the biosynthesis of unsaturated fatty acids, and ABC transporters were upregulated in resistant isolates. Our findings show an increment of tetracosanoic (myristic) acid suggesting a possible β-tubulin-compensation mechanism in resistant isolates. The presence of myristic acid promoted the generation of diacylglycerol kinase δ which facilitated the production of β-tubulin in other studies. Additionally, important changes in the metabolite profiles were observed as soon as six hours after exposure to the fungicide showing an early response of the pathogen. To the best of our knowledge, this is the first report that describes the changes in the metabolite profile of P. fijiensis resistant to thiabendazole when exposed to the fungicide.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0313915</identifier><identifier>PMID: 39570826</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Acid resistance ; Analysis ; Ascomycota - drug effects ; Ascomycota - metabolism ; Banana ; Bananas ; Biology and Life Sciences ; Biosynthesis ; Black Sigatoka ; Care and treatment ; Causes of ; D-Sorbitol ; Diacylglycerol kinase ; Disease control ; Disease resistance ; Diseases and pests ; Drug resistance in microorganisms ; Drug Resistance, Fungal - drug effects ; Enzymes ; Ethylenediaminetetraacetic acid ; Experiments ; Fructose ; Fungal diseases of plants ; Fungicides ; Fungicides, Industrial - pharmacology ; Gas Chromatography-Mass Spectrometry ; Growth ; Kinases ; Mannose ; Metabolic pathways ; Metabolites ; Metabolome - drug effects ; Musa - metabolism ; Musa - microbiology ; Palmitic acid ; Pathogens ; Pesticides ; Phosphoric acid ; Physical Sciences ; Plant diseases ; Plant Diseases - microbiology ; Plant layout ; Plant metabolites ; Proline ; Pseudocercospora fijiensis ; Research and Analysis Methods ; Saturated fatty acids ; Sorbitol ; Thiabendazole ; Thiabendazole - pharmacology ; Tubulin ; Tubulins ; Unsaturated fatty acids</subject><ispartof>PloS one, 2024-11, Vol.19 (11), p.e0313915</ispartof><rights>Copyright: © 2024 Maridueña-Zavala et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.</rights><rights>COPYRIGHT 2024 Public Library of Science</rights><rights>2024 Maridueña-Zavala et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2024 Maridueña-Zavala et al 2024 Maridueña-Zavala et al</rights><rights>2024 Maridueña-Zavala et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c4875-1c04644d57c3b60780a64b743fa0a9cc5ce0a9f381e3c8b9a20528f03bf43243</cites><orcidid>0000-0002-4327-0030 ; 0000-0003-4609-7998</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC11581298/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC11581298/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,2102,2928,23866,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/39570826$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Kniemeyer, Olaf</contributor><creatorcontrib>Maridueña-Zavala, María Gabriela</creatorcontrib><creatorcontrib>Chong-Aguirre, Pablo Antonio</creatorcontrib><creatorcontrib>Freire-Peñaherrera, Andrea</creatorcontrib><creatorcontrib>Moreno, Arturo</creatorcontrib><creatorcontrib>Reyes-De-Corcuera, José Ignacio</creatorcontrib><creatorcontrib>Jiménez-Feijoo, María Isabel</creatorcontrib><creatorcontrib>Cevallos-Cevallos, Juan Manuel</creatorcontrib><title>GC-MS metabolite profiling of Pseudocercospora fijiensis isolates resistant to thiabendazole</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Black Sigatoka is the most widespread banana disease worldwide. It is caused by Pseudocercospora fijiensis, a fungal pathogen known for developing resistance to fungicides such as thiabendazole. Despite the increasing costs associated with the use of chemicals to control this disease, the pathogen's mechanisms for fungicide resistance are not fully understood. The metabolite profiles of P. fijiensis isolates with different levels of resistance to thiabendazole were characterized by GC-MS. A total of 33 isolates were obtained from symptomatic banana plants and the sensitivity of each isolate to thiabendazole was assessed at 0, 1, 10, 100, 1000, and 10000 μg.mL-1. Then, the metabolite profile of each isolate was assessed using GC-MS. Metabolites such as hexadecanoic acid, tetradecanoic acid, octadecadienoic acid and octadecanoic acid were significantly over-accumulated in the presence of thiabendazole at 10 μg.mL-1. Phosphoric acid, L-proline, and D-allose increased in concentration with time in the presence of 100 μg.mL-1 of thiabendazole, and mannonic acid, 1-hexadecanol, D-sorbitol and tetracosanoic acid were only detected in the presence of the fungicide. Metabolic pathways including that of fructose, mannose metabolism, the biosynthesis of unsaturated fatty acids, and ABC transporters were upregulated in resistant isolates. Our findings show an increment of tetracosanoic (myristic) acid suggesting a possible β-tubulin-compensation mechanism in resistant isolates. The presence of myristic acid promoted the generation of diacylglycerol kinase δ which facilitated the production of β-tubulin in other studies. Additionally, important changes in the metabolite profiles were observed as soon as six hours after exposure to the fungicide showing an early response of the pathogen. To the best of our knowledge, this is the first report that describes the changes in the metabolite profile of P. fijiensis resistant to thiabendazole when exposed to the fungicide.</description><subject>Acid resistance</subject><subject>Analysis</subject><subject>Ascomycota - drug effects</subject><subject>Ascomycota - metabolism</subject><subject>Banana</subject><subject>Bananas</subject><subject>Biology and Life Sciences</subject><subject>Biosynthesis</subject><subject>Black Sigatoka</subject><subject>Care and treatment</subject><subject>Causes of</subject><subject>D-Sorbitol</subject><subject>Diacylglycerol kinase</subject><subject>Disease control</subject><subject>Disease resistance</subject><subject>Diseases and pests</subject><subject>Drug resistance in microorganisms</subject><subject>Drug Resistance, Fungal - drug effects</subject><subject>Enzymes</subject><subject>Ethylenediaminetetraacetic acid</subject><subject>Experiments</subject><subject>Fructose</subject><subject>Fungal diseases of plants</subject><subject>Fungicides</subject><subject>Fungicides, Industrial - pharmacology</subject><subject>Gas Chromatography-Mass Spectrometry</subject><subject>Growth</subject><subject>Kinases</subject><subject>Mannose</subject><subject>Metabolic pathways</subject><subject>Metabolites</subject><subject>Metabolome - drug effects</subject><subject>Musa - metabolism</subject><subject>Musa - microbiology</subject><subject>Palmitic acid</subject><subject>Pathogens</subject><subject>Pesticides</subject><subject>Phosphoric acid</subject><subject>Physical Sciences</subject><subject>Plant diseases</subject><subject>Plant Diseases - microbiology</subject><subject>Plant layout</subject><subject>Plant metabolites</subject><subject>Proline</subject><subject>Pseudocercospora fijiensis</subject><subject>Research and Analysis Methods</subject><subject>Saturated fatty acids</subject><subject>Sorbitol</subject><subject>Thiabendazole</subject><subject>Thiabendazole - pharmacology</subject><subject>Tubulin</subject><subject>Tubulins</subject><subject>Unsaturated fatty acids</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>DOA</sourceid><recordid>eNqNk1Fv0zAQxyMEYmPwDRBEQkLw0GLHTuI8oamCUWloiE08IVkX59y6cuPOdhDw6XFpNi1oDygPju3f_c_--y7LnlMyp6ym7zZu8D3Y-c71OCeMsoaWD7Jj2rBiVhWEPbzzf5Q9CWFDSMlEVT3OjlhT1kQU1XH2_Wwx-3yZbzFC66yJmO-808aafpU7nX8JOHROoVcu7JyHXJuNwT6YkJvgLEQMucc0jdDHPLo8rg202Hfw21l8mj3SYAM-G8eT7Orjh6vFp9n5xdlycXo-U1zU5YwqwivOu7JWrK1ILQhUvK0500CgUapUmEbNBEWmRNtAQcpCaMJazVnB2Un28iC7sy7I0ZcgkyW0rqumIolYHojOwUbuvNmC_yUdGPl3wfmVBB-NsiiFQOzKlIiULW8ZE1C0rINknQZR1fts78dsQ7vFTmEfPdiJ6HSnN2u5cj8kpaWgRSOSwptRwbvrAUOUWxMUWgs9uuFwcMEFY1VCX_2D3n-9kVpBuoHptUuJ1V5UngpaE55s2mvN76HS1-HWqFRF6dlxGvB2EpCYiD_jCoYQ5PLy6_-zF9-m7Os77BrBxnUqpiEa14cpyA-g8i4Ej_rWZUrkvglu3JD7JpBjE6SwF3df6DbopurZH9svAZc</recordid><startdate>20241121</startdate><enddate>20241121</enddate><creator>Maridueña-Zavala, María Gabriela</creator><creator>Chong-Aguirre, Pablo Antonio</creator><creator>Freire-Peñaherrera, Andrea</creator><creator>Moreno, Arturo</creator><creator>Reyes-De-Corcuera, José Ignacio</creator><creator>Jiménez-Feijoo, María Isabel</creator><creator>Cevallos-Cevallos, Juan Manuel</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0002-4327-0030</orcidid><orcidid>https://orcid.org/0000-0003-4609-7998</orcidid></search><sort><creationdate>20241121</creationdate><title>GC-MS metabolite profiling of Pseudocercospora fijiensis isolates resistant to thiabendazole</title><author>Maridueña-Zavala, María Gabriela ; Chong-Aguirre, Pablo Antonio ; Freire-Peñaherrera, Andrea ; Moreno, Arturo ; Reyes-De-Corcuera, José Ignacio ; Jiménez-Feijoo, María Isabel ; Cevallos-Cevallos, Juan Manuel</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4875-1c04644d57c3b60780a64b743fa0a9cc5ce0a9f381e3c8b9a20528f03bf43243</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Acid resistance</topic><topic>Analysis</topic><topic>Ascomycota - drug effects</topic><topic>Ascomycota - metabolism</topic><topic>Banana</topic><topic>Bananas</topic><topic>Biology and Life Sciences</topic><topic>Biosynthesis</topic><topic>Black Sigatoka</topic><topic>Care and treatment</topic><topic>Causes of</topic><topic>D-Sorbitol</topic><topic>Diacylglycerol kinase</topic><topic>Disease control</topic><topic>Disease resistance</topic><topic>Diseases and pests</topic><topic>Drug resistance in microorganisms</topic><topic>Drug Resistance, Fungal - drug effects</topic><topic>Enzymes</topic><topic>Ethylenediaminetetraacetic acid</topic><topic>Experiments</topic><topic>Fructose</topic><topic>Fungal diseases of plants</topic><topic>Fungicides</topic><topic>Fungicides, Industrial - pharmacology</topic><topic>Gas Chromatography-Mass Spectrometry</topic><topic>Growth</topic><topic>Kinases</topic><topic>Mannose</topic><topic>Metabolic pathways</topic><topic>Metabolites</topic><topic>Metabolome - drug effects</topic><topic>Musa - metabolism</topic><topic>Musa - microbiology</topic><topic>Palmitic acid</topic><topic>Pathogens</topic><topic>Pesticides</topic><topic>Phosphoric acid</topic><topic>Physical Sciences</topic><topic>Plant diseases</topic><topic>Plant Diseases - microbiology</topic><topic>Plant layout</topic><topic>Plant metabolites</topic><topic>Proline</topic><topic>Pseudocercospora fijiensis</topic><topic>Research and Analysis Methods</topic><topic>Saturated fatty acids</topic><topic>Sorbitol</topic><topic>Thiabendazole</topic><topic>Thiabendazole - pharmacology</topic><topic>Tubulin</topic><topic>Tubulins</topic><topic>Unsaturated fatty acids</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Maridueña-Zavala, María Gabriela</creatorcontrib><creatorcontrib>Chong-Aguirre, Pablo Antonio</creatorcontrib><creatorcontrib>Freire-Peñaherrera, Andrea</creatorcontrib><creatorcontrib>Moreno, Arturo</creatorcontrib><creatorcontrib>Reyes-De-Corcuera, José Ignacio</creatorcontrib><creatorcontrib>Jiménez-Feijoo, María Isabel</creatorcontrib><creatorcontrib>Cevallos-Cevallos, Juan Manuel</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: Opposing Viewpoints</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Nursing & Allied Health Database</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Meteorological & Geoastrophysical Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Meteorological & Geoastrophysical Abstracts - Academic</collection><collection>ProQuest Engineering Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Engineering Database</collection><collection>Nursing & Allied Health Premium</collection><collection>Advanced Technologies & Aerospace Database</collection><collection>ProQuest Advanced Technologies & Aerospace Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>Materials Science Collection</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Engineering Collection</collection><collection>Environmental Science Collection</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Maridueña-Zavala, María Gabriela</au><au>Chong-Aguirre, Pablo Antonio</au><au>Freire-Peñaherrera, Andrea</au><au>Moreno, Arturo</au><au>Reyes-De-Corcuera, José Ignacio</au><au>Jiménez-Feijoo, María Isabel</au><au>Cevallos-Cevallos, Juan Manuel</au><au>Kniemeyer, Olaf</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>GC-MS metabolite profiling of Pseudocercospora fijiensis isolates resistant to thiabendazole</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2024-11-21</date><risdate>2024</risdate><volume>19</volume><issue>11</issue><spage>e0313915</spage><pages>e0313915-</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Black Sigatoka is the most widespread banana disease worldwide. It is caused by Pseudocercospora fijiensis, a fungal pathogen known for developing resistance to fungicides such as thiabendazole. Despite the increasing costs associated with the use of chemicals to control this disease, the pathogen's mechanisms for fungicide resistance are not fully understood. The metabolite profiles of P. fijiensis isolates with different levels of resistance to thiabendazole were characterized by GC-MS. A total of 33 isolates were obtained from symptomatic banana plants and the sensitivity of each isolate to thiabendazole was assessed at 0, 1, 10, 100, 1000, and 10000 μg.mL-1. Then, the metabolite profile of each isolate was assessed using GC-MS. Metabolites such as hexadecanoic acid, tetradecanoic acid, octadecadienoic acid and octadecanoic acid were significantly over-accumulated in the presence of thiabendazole at 10 μg.mL-1. Phosphoric acid, L-proline, and D-allose increased in concentration with time in the presence of 100 μg.mL-1 of thiabendazole, and mannonic acid, 1-hexadecanol, D-sorbitol and tetracosanoic acid were only detected in the presence of the fungicide. Metabolic pathways including that of fructose, mannose metabolism, the biosynthesis of unsaturated fatty acids, and ABC transporters were upregulated in resistant isolates. Our findings show an increment of tetracosanoic (myristic) acid suggesting a possible β-tubulin-compensation mechanism in resistant isolates. The presence of myristic acid promoted the generation of diacylglycerol kinase δ which facilitated the production of β-tubulin in other studies. Additionally, important changes in the metabolite profiles were observed as soon as six hours after exposure to the fungicide showing an early response of the pathogen. To the best of our knowledge, this is the first report that describes the changes in the metabolite profile of P. fijiensis resistant to thiabendazole when exposed to the fungicide.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>39570826</pmid><doi>10.1371/journal.pone.0313915</doi><tpages>e0313915</tpages><orcidid>https://orcid.org/0000-0002-4327-0030</orcidid><orcidid>https://orcid.org/0000-0003-4609-7998</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2024-11, Vol.19 (11), p.e0313915 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_3131776960 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Public Library of Science (PLoS) Journals Open Access; EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Acid resistance Analysis Ascomycota - drug effects Ascomycota - metabolism Banana Bananas Biology and Life Sciences Biosynthesis Black Sigatoka Care and treatment Causes of D-Sorbitol Diacylglycerol kinase Disease control Disease resistance Diseases and pests Drug resistance in microorganisms Drug Resistance, Fungal - drug effects Enzymes Ethylenediaminetetraacetic acid Experiments Fructose Fungal diseases of plants Fungicides Fungicides, Industrial - pharmacology Gas Chromatography-Mass Spectrometry Growth Kinases Mannose Metabolic pathways Metabolites Metabolome - drug effects Musa - metabolism Musa - microbiology Palmitic acid Pathogens Pesticides Phosphoric acid Physical Sciences Plant diseases Plant Diseases - microbiology Plant layout Plant metabolites Proline Pseudocercospora fijiensis Research and Analysis Methods Saturated fatty acids Sorbitol Thiabendazole Thiabendazole - pharmacology Tubulin Tubulins Unsaturated fatty acids |
| title | GC-MS metabolite profiling of Pseudocercospora fijiensis isolates resistant to thiabendazole |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-24T03%3A05%3A59IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=GC-MS%20metabolite%20profiling%20of%20Pseudocercospora%20fijiensis%20isolates%20resistant%20to%20thiabendazole&rft.jtitle=PloS%20one&rft.au=Maridue%C3%B1a-Zavala,%20Mar%C3%ADa%20Gabriela&rft.date=2024-11-21&rft.volume=19&rft.issue=11&rft.spage=e0313915&rft.pages=e0313915-&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0313915&rft_dat=%3Cgale_plos_%3EA817044326%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=3131776960&rft_id=info:pmid/39570826&rft_galeid=A817044326&rft_doaj_id=oai_doaj_org_article_88eed5e3c05b4b338a2b3da053fa8674&rfr_iscdi=true |