Genomic variability in Zika virus in GBS cases in Colombia
Major clusters of Guillain-Barré Syndrome (GBS) emerged during the Zika virus (ZIKV) outbreaks in the South Pacific and the Americas from 2014 to 2016. The factors contributing to GBS susceptibility in ZIKV infection remain unclear, although considerations of viral variation, patient susceptibility,...
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| description | Major clusters of Guillain-Barré Syndrome (GBS) emerged during the Zika virus (ZIKV) outbreaks in the South Pacific and the Americas from 2014 to 2016. The factors contributing to GBS susceptibility in ZIKV infection remain unclear, although considerations of viral variation, patient susceptibility, environmental influences, and other potential factors have been hypothesized. Studying the role of viral genetic factors has been challenging due to the low viral load and rapid viral clearance from the blood after the onset of Zika symptoms. The prolonged excretion of ZIKV in urine by the time of GBS onset, when the virus is no longer present in the blood, provides an opportunity to unravel whether specific ZIKV mutations are related to the development of GBS in certain individuals. This study aimed to investigate the association between specific ZIKV genotypes and the development of GBS, taking advantage of a unique collection of ZIKV-positive urine samples obtained from GBS cases and controls during the 2016 ZIKV outbreak in Colombia. Utilizing Oxford-Nanopore technology, we conducted complete genome sequencing of ZIKV in biological samples from 15 patients with GBS associated with ZIKV and 17 with ZIKV infection without neurological complications. ZIKV genotypes in Colombia exhibited distribution across three clades (average bootstrap of 90.9±14.9%), with two clades dominating the landscape. A comparative analysis of ZIKV genomes from GBS and non-neurological complications, alongside 1368 previously reported genomes, revealed no significant distinctions between the two groups. Both genotypes were similarly distributed among observed clades in Colombia. Furthermore, no variations were identified in the amino acid composition of the viral genome between the two groups. Our findings suggest that GBS in ZIKV infection is perhaps associated with patient susceptibility and/or other para- or post-infectious immune-mediated mechanisms rather than with specific ZIKV genome variations. |
| doi_str_mv | 10.1371/journal.pone.0313545 |
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The factors contributing to GBS susceptibility in ZIKV infection remain unclear, although considerations of viral variation, patient susceptibility, environmental influences, and other potential factors have been hypothesized. Studying the role of viral genetic factors has been challenging due to the low viral load and rapid viral clearance from the blood after the onset of Zika symptoms. The prolonged excretion of ZIKV in urine by the time of GBS onset, when the virus is no longer present in the blood, provides an opportunity to unravel whether specific ZIKV mutations are related to the development of GBS in certain individuals. This study aimed to investigate the association between specific ZIKV genotypes and the development of GBS, taking advantage of a unique collection of ZIKV-positive urine samples obtained from GBS cases and controls during the 2016 ZIKV outbreak in Colombia. Utilizing Oxford-Nanopore technology, we conducted complete genome sequencing of ZIKV in biological samples from 15 patients with GBS associated with ZIKV and 17 with ZIKV infection without neurological complications. ZIKV genotypes in Colombia exhibited distribution across three clades (average bootstrap of 90.9±14.9%), with two clades dominating the landscape. A comparative analysis of ZIKV genomes from GBS and non-neurological complications, alongside 1368 previously reported genomes, revealed no significant distinctions between the two groups. Both genotypes were similarly distributed among observed clades in Colombia. Furthermore, no variations were identified in the amino acid composition of the viral genome between the two groups. Our findings suggest that GBS in ZIKV infection is perhaps associated with patient susceptibility and/or other para- or post-infectious immune-mediated mechanisms rather than with specific ZIKV genome variations.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0313545</identifier><identifier>PMID: 39561198</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Adult ; Amino acid composition ; Amino acids ; Analysis ; Bacterial infections ; Biological properties ; Biological samples ; Biology and life sciences ; Biotechnology industry ; Blood ; Colombia - epidemiology ; Comparative analysis ; Computer and Information Sciences ; Diagnosis ; Disease Outbreaks ; Disease susceptibility ; DNA sequencing ; Epidemics ; Female ; Gene sequencing ; Genes ; Genetic aspects ; Genetic factors ; Genetic Variation ; Genome, Viral - genetics ; Genomes ; Genomics ; Genotype ; Genotypes ; Guillain-Barre syndrome ; Guillain-Barre Syndrome - epidemiology ; Guillain-Barre Syndrome - virology ; Health aspects ; Humans ; Immune clearance ; Infection ; Infections ; Male ; Maximum likelihood method ; Medical research ; Medicine and Health Sciences ; Medicine, Experimental ; Microcephaly ; Middle Aged ; Mosquitoes ; Neurological complications ; Nucleotide sequencing ; Outbreaks ; People and places ; Phylogenetics ; Phylogeny ; Risk factors ; Urine ; Variation ; Vector-borne diseases ; Viral genetics ; Viruses ; Young Adult ; Zika virus ; Zika Virus - genetics ; Zika Virus - isolation & purification ; Zika Virus Infection - epidemiology ; Zika Virus Infection - urine ; Zika Virus Infection - virology</subject><ispartof>PloS one, 2024-11, Vol.19 (11), p.e0313545</ispartof><rights>Copyright: © 2024 Rivera-Franco et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.</rights><rights>COPYRIGHT 2024 Public Library of Science</rights><rights>2024 Rivera-Franco et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2024 Rivera-Franco et al 2024 Rivera-Franco et al</rights><rights>2024 Rivera-Franco et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c572t-2132817d8b3b07a3ebcd14cebce934ef71ec4a6a176287f433d4ed8d6f82ae123</cites><orcidid>0000-0002-8859-7432 ; 0000-0002-5371-7449</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC11575819/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC11575819/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,724,777,781,861,882,2096,2915,23847,27905,27906,53772,53774,79349,79350</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/39561198$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Ruiz-Saenz, Julian</contributor><creatorcontrib>Rivera-Franco, Nelson</creatorcontrib><creatorcontrib>López-Alvarez, Diana</creatorcontrib><creatorcontrib>Castillo, Andrés</creatorcontrib><creatorcontrib>Aristizabal, Erica</creatorcontrib><creatorcontrib>Puiu, Daniela</creatorcontrib><creatorcontrib>Salzberg, Steven L</creatorcontrib><creatorcontrib>Pardo, Carlos A</creatorcontrib><creatorcontrib>Parra, Beatriz</creatorcontrib><creatorcontrib>NEAS</creatorcontrib><creatorcontrib>on behalf of NEAS</creatorcontrib><title>Genomic variability in Zika virus in GBS cases in Colombia</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Major clusters of Guillain-Barré Syndrome (GBS) emerged during the Zika virus (ZIKV) outbreaks in the South Pacific and the Americas from 2014 to 2016. The factors contributing to GBS susceptibility in ZIKV infection remain unclear, although considerations of viral variation, patient susceptibility, environmental influences, and other potential factors have been hypothesized. Studying the role of viral genetic factors has been challenging due to the low viral load and rapid viral clearance from the blood after the onset of Zika symptoms. The prolonged excretion of ZIKV in urine by the time of GBS onset, when the virus is no longer present in the blood, provides an opportunity to unravel whether specific ZIKV mutations are related to the development of GBS in certain individuals. This study aimed to investigate the association between specific ZIKV genotypes and the development of GBS, taking advantage of a unique collection of ZIKV-positive urine samples obtained from GBS cases and controls during the 2016 ZIKV outbreak in Colombia. Utilizing Oxford-Nanopore technology, we conducted complete genome sequencing of ZIKV in biological samples from 15 patients with GBS associated with ZIKV and 17 with ZIKV infection without neurological complications. ZIKV genotypes in Colombia exhibited distribution across three clades (average bootstrap of 90.9±14.9%), with two clades dominating the landscape. A comparative analysis of ZIKV genomes from GBS and non-neurological complications, alongside 1368 previously reported genomes, revealed no significant distinctions between the two groups. Both genotypes were similarly distributed among observed clades in Colombia. Furthermore, no variations were identified in the amino acid composition of the viral genome between the two groups. 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Rivera-Franco, Nelson</au><au>López-Alvarez, Diana</au><au>Castillo, Andrés</au><au>Aristizabal, Erica</au><au>Puiu, Daniela</au><au>Salzberg, Steven L</au><au>Pardo, Carlos A</au><au>Parra, Beatriz</au><au>Ruiz-Saenz, Julian</au><aucorp>NEAS</aucorp><aucorp>on behalf of NEAS</aucorp><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Genomic variability in Zika virus in GBS cases in Colombia</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2024-11-19</date><risdate>2024</risdate><volume>19</volume><issue>11</issue><spage>e0313545</spage><pages>e0313545-</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Major clusters of Guillain-Barré Syndrome (GBS) emerged during the Zika virus (ZIKV) outbreaks in the South Pacific and the Americas from 2014 to 2016. The factors contributing to GBS susceptibility in ZIKV infection remain unclear, although considerations of viral variation, patient susceptibility, environmental influences, and other potential factors have been hypothesized. Studying the role of viral genetic factors has been challenging due to the low viral load and rapid viral clearance from the blood after the onset of Zika symptoms. The prolonged excretion of ZIKV in urine by the time of GBS onset, when the virus is no longer present in the blood, provides an opportunity to unravel whether specific ZIKV mutations are related to the development of GBS in certain individuals. This study aimed to investigate the association between specific ZIKV genotypes and the development of GBS, taking advantage of a unique collection of ZIKV-positive urine samples obtained from GBS cases and controls during the 2016 ZIKV outbreak in Colombia. Utilizing Oxford-Nanopore technology, we conducted complete genome sequencing of ZIKV in biological samples from 15 patients with GBS associated with ZIKV and 17 with ZIKV infection without neurological complications. ZIKV genotypes in Colombia exhibited distribution across three clades (average bootstrap of 90.9±14.9%), with two clades dominating the landscape. A comparative analysis of ZIKV genomes from GBS and non-neurological complications, alongside 1368 previously reported genomes, revealed no significant distinctions between the two groups. Both genotypes were similarly distributed among observed clades in Colombia. Furthermore, no variations were identified in the amino acid composition of the viral genome between the two groups. Our findings suggest that GBS in ZIKV infection is perhaps associated with patient susceptibility and/or other para- or post-infectious immune-mediated mechanisms rather than with specific ZIKV genome variations.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>39561198</pmid><doi>10.1371/journal.pone.0313545</doi><tpages>e0313545</tpages><orcidid>https://orcid.org/0000-0002-8859-7432</orcidid><orcidid>https://orcid.org/0000-0002-5371-7449</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2024-11, Vol.19 (11), p.e0313545 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_3130600143 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Public Library of Science (PLoS); PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Adult Amino acid composition Amino acids Analysis Bacterial infections Biological properties Biological samples Biology and life sciences Biotechnology industry Blood Colombia - epidemiology Comparative analysis Computer and Information Sciences Diagnosis Disease Outbreaks Disease susceptibility DNA sequencing Epidemics Female Gene sequencing Genes Genetic aspects Genetic factors Genetic Variation Genome, Viral - genetics Genomes Genomics Genotype Genotypes Guillain-Barre syndrome Guillain-Barre Syndrome - epidemiology Guillain-Barre Syndrome - virology Health aspects Humans Immune clearance Infection Infections Male Maximum likelihood method Medical research Medicine and Health Sciences Medicine, Experimental Microcephaly Middle Aged Mosquitoes Neurological complications Nucleotide sequencing Outbreaks People and places Phylogenetics Phylogeny Risk factors Urine Variation Vector-borne diseases Viral genetics Viruses Young Adult Zika virus Zika Virus - genetics Zika Virus - isolation & purification Zika Virus Infection - epidemiology Zika Virus Infection - urine Zika Virus Infection - virology |
| title | Genomic variability in Zika virus in GBS cases in Colombia |
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