Increased QPCT gene expression by the hepatitis B virus promotes HBV replication
Glutamine cyclase, an enzyme involved in posttranslational modifications, is encoded by the glutaminyl-peptide cyclotransferase (QPCT) gene. Gene microarray analysis revealed that the QPCT gene was highly expressed in HepG2.2.15 cells compared with that in HepG2 cells. The serum expression level of...
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| creator | Zhang, Conghui Ma, Qingfeng Wang, Wei Song, Hui Wang, Xue Xu, Fengxia Zhu, Chengliang Liu, Xinghui |
| description | Glutamine cyclase, an enzyme involved in posttranslational modifications, is encoded by the glutaminyl-peptide cyclotransferase (QPCT) gene. Gene microarray analysis revealed that the QPCT gene was highly expressed in HepG2.2.15 cells compared with that in HepG2 cells. The serum expression level of the QPCT gene was detected by ELISA and was significantly greater in HBV-infected patients than in healthy controls. The mRNA and protein expression levels of the QPCT gene were markedly greater in the HBV-expressing cell lines (HepG2.2.15, and HepG2 and Huh7 cells transfected with the pBlu-HBV plasmid) than in the HepG2 and Huh7 cells. The levels of HBV pgRNA and HBV-DNA copy number, as well as the levels of HBeAg and HBsAg, also increased in the HepG2 and Huh7 cell lines cotransfected with the QPCT gene expression plasmid and the HBV 1.3-fold plasmid. Our study indicated that HBV can promote the expression of the QPCT gene, which in turn promotes the expression and replication of HBV. |
| doi_str_mv | 10.1371/journal.pone.0312773 |
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Gene microarray analysis revealed that the QPCT gene was highly expressed in HepG2.2.15 cells compared with that in HepG2 cells. The serum expression level of the QPCT gene was detected by ELISA and was significantly greater in HBV-infected patients than in healthy controls. The mRNA and protein expression levels of the QPCT gene were markedly greater in the HBV-expressing cell lines (HepG2.2.15, and HepG2 and Huh7 cells transfected with the pBlu-HBV plasmid) than in the HepG2 and Huh7 cells. The levels of HBV pgRNA and HBV-DNA copy number, as well as the levels of HBeAg and HBsAg, also increased in the HepG2 and Huh7 cell lines cotransfected with the QPCT gene expression plasmid and the HBV 1.3-fold plasmid. Our study indicated that HBV can promote the expression of the QPCT gene, which in turn promotes the expression and replication of HBV.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0312773</identifier><identifier>PMID: 39531472</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Adult ; Analysis ; Antigens ; Bioinformatics ; Biology and life sciences ; Cell lines ; Comparative analysis ; Copy number ; Diagnosis ; DNA microarrays ; DNA, Viral - genetics ; Enzyme-linked immunosorbent assay ; Enzymes ; Female ; Gene expression ; Genes ; Genetic aspects ; Genomes ; Glutamine ; Glutaminyl-peptide cyclotransferase ; Health care ; Hep G2 Cells ; Hepatitis B ; Hepatitis B - genetics ; Hepatitis B - virology ; Hepatitis B e antigen ; Hepatitis B e Antigens - genetics ; Hepatitis B e Antigens - metabolism ; Hepatitis B surface antigen ; Hepatitis B Surface Antigens - genetics ; Hepatitis B Surface Antigens - metabolism ; Hepatitis B virus - genetics ; Hepatitis B virus - physiology ; Humans ; Infections ; Liver cancer ; Male ; Medicine and Health Sciences ; Messenger RNA ; Middle Aged ; Mortality ; Plasmids ; Proteins ; Replication ; Research and Analysis Methods ; Statistical analysis ; Virus Replication - genetics ; Viruses</subject><ispartof>PloS one, 2024-11, Vol.19 (11), p.e0312773</ispartof><rights>Copyright: © 2024 Zhang et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.</rights><rights>COPYRIGHT 2024 Public Library of Science</rights><rights>2024 Zhang et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2024 Zhang et al 2024 Zhang et al</rights><rights>2024 Zhang et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c526t-9c01895dc69a92610bd02d730113fe3dc5d16a978668f68bc08e560586364f743</cites><orcidid>0000-0002-1676-3235</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC11556691/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC11556691/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,2096,2915,23845,27901,27902,53766,53768,79343,79344</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/39531472$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Domfeh, Seth Agyei</contributor><creatorcontrib>Zhang, Conghui</creatorcontrib><creatorcontrib>Ma, Qingfeng</creatorcontrib><creatorcontrib>Wang, Wei</creatorcontrib><creatorcontrib>Song, Hui</creatorcontrib><creatorcontrib>Wang, Xue</creatorcontrib><creatorcontrib>Xu, Fengxia</creatorcontrib><creatorcontrib>Zhu, Chengliang</creatorcontrib><creatorcontrib>Liu, Xinghui</creatorcontrib><title>Increased QPCT gene expression by the hepatitis B virus promotes HBV replication</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Glutamine cyclase, an enzyme involved in posttranslational modifications, is encoded by the glutaminyl-peptide cyclotransferase (QPCT) gene. Gene microarray analysis revealed that the QPCT gene was highly expressed in HepG2.2.15 cells compared with that in HepG2 cells. The serum expression level of the QPCT gene was detected by ELISA and was significantly greater in HBV-infected patients than in healthy controls. The mRNA and protein expression levels of the QPCT gene were markedly greater in the HBV-expressing cell lines (HepG2.2.15, and HepG2 and Huh7 cells transfected with the pBlu-HBV plasmid) than in the HepG2 and Huh7 cells. The levels of HBV pgRNA and HBV-DNA copy number, as well as the levels of HBeAg and HBsAg, also increased in the HepG2 and Huh7 cell lines cotransfected with the QPCT gene expression plasmid and the HBV 1.3-fold plasmid. Our study indicated that HBV can promote the expression of the QPCT gene, which in turn promotes the expression and replication of HBV.</description><subject>Adult</subject><subject>Analysis</subject><subject>Antigens</subject><subject>Bioinformatics</subject><subject>Biology and life sciences</subject><subject>Cell lines</subject><subject>Comparative analysis</subject><subject>Copy number</subject><subject>Diagnosis</subject><subject>DNA microarrays</subject><subject>DNA, Viral - genetics</subject><subject>Enzyme-linked immunosorbent assay</subject><subject>Enzymes</subject><subject>Female</subject><subject>Gene expression</subject><subject>Genes</subject><subject>Genetic aspects</subject><subject>Genomes</subject><subject>Glutamine</subject><subject>Glutaminyl-peptide cyclotransferase</subject><subject>Health care</subject><subject>Hep G2 Cells</subject><subject>Hepatitis B</subject><subject>Hepatitis B - genetics</subject><subject>Hepatitis B - virology</subject><subject>Hepatitis B e antigen</subject><subject>Hepatitis B e Antigens - genetics</subject><subject>Hepatitis B e Antigens - metabolism</subject><subject>Hepatitis B surface antigen</subject><subject>Hepatitis B Surface Antigens - genetics</subject><subject>Hepatitis B Surface Antigens - metabolism</subject><subject>Hepatitis B virus - genetics</subject><subject>Hepatitis B virus - physiology</subject><subject>Humans</subject><subject>Infections</subject><subject>Liver cancer</subject><subject>Male</subject><subject>Medicine and Health Sciences</subject><subject>Messenger RNA</subject><subject>Middle Aged</subject><subject>Mortality</subject><subject>Plasmids</subject><subject>Proteins</subject><subject>Replication</subject><subject>Research and Analysis Methods</subject><subject>Statistical analysis</subject><subject>Virus Replication - genetics</subject><subject>Viruses</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><sourceid>DOA</sourceid><recordid>eNqNksFu1DAQhiMEoqXwBggiISE47GLHsR2fULsCulKlFii9Wo492fUqG6d2UrVvj9NNqw3qAflga_zNP-PxnyRvMZpjwvGXjet9o-p56xqYI4Izzsmz5BALks1YhsjzvfNB8iqEDUKUFIy9TA6IoATnPDtMLpaN9qACmPTnxeIyXUEDKdy2HkKwrknLu7RbQ7qGVnW2syE9SW-s70Paerd1HYT09OQq9dDWVkfCNa-TF5WqA7wZ96Pkz_dvl4vT2dn5j-Xi-Gymaca6mdAIF4IazYQSGcOoNCgznCCMSQXEaGowU4LHfouKFaVGBVCGaMEIyyuek6Pk_U63rV2Q4zCCHOZACWdCRGK5I4xTG9l6u1X-Tjpl5X3A-ZVUvrO6BqkyiNVzigypcqOrshIsMwwXCCmBKh61vo7V-nILRkPTeVVPRKc3jV3LlbuRGFPKmMBR4dOo4N11D6GTWxs01LVqwPX3jRecoSIf0A__oE8_b6RWKr7ANpWLhfUgKo8LTHmOMsYiNX-CisvA1uponcrG-CTh8yQhMh3cdivVhyCXv3_9P3t-NWU_7rFrUHW3Dq7uB8-EKZjvQO1dCB6qxyljJAfnP0xDDs6Xo_Nj2rv9H3pMerA6-QuG-Pu1</recordid><startdate>20241112</startdate><enddate>20241112</enddate><creator>Zhang, Conghui</creator><creator>Ma, Qingfeng</creator><creator>Wang, Wei</creator><creator>Song, Hui</creator><creator>Wang, Xue</creator><creator>Xu, Fengxia</creator><creator>Zhu, Chengliang</creator><creator>Liu, Xinghui</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0002-1676-3235</orcidid></search><sort><creationdate>20241112</creationdate><title>Increased QPCT gene expression by the hepatitis B virus promotes HBV replication</title><author>Zhang, Conghui ; Ma, Qingfeng ; Wang, Wei ; Song, Hui ; Wang, Xue ; Xu, Fengxia ; Zhu, Chengliang ; Liu, Xinghui</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c526t-9c01895dc69a92610bd02d730113fe3dc5d16a978668f68bc08e560586364f743</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Adult</topic><topic>Analysis</topic><topic>Antigens</topic><topic>Bioinformatics</topic><topic>Biology and life sciences</topic><topic>Cell lines</topic><topic>Comparative analysis</topic><topic>Copy number</topic><topic>Diagnosis</topic><topic>DNA microarrays</topic><topic>DNA, Viral - genetics</topic><topic>Enzyme-linked immunosorbent assay</topic><topic>Enzymes</topic><topic>Female</topic><topic>Gene expression</topic><topic>Genes</topic><topic>Genetic aspects</topic><topic>Genomes</topic><topic>Glutamine</topic><topic>Glutaminyl-peptide cyclotransferase</topic><topic>Health care</topic><topic>Hep G2 Cells</topic><topic>Hepatitis B</topic><topic>Hepatitis B - genetics</topic><topic>Hepatitis B - virology</topic><topic>Hepatitis B e antigen</topic><topic>Hepatitis B e Antigens - genetics</topic><topic>Hepatitis B e Antigens - metabolism</topic><topic>Hepatitis B surface antigen</topic><topic>Hepatitis B Surface Antigens - genetics</topic><topic>Hepatitis B Surface Antigens - metabolism</topic><topic>Hepatitis B virus - genetics</topic><topic>Hepatitis B virus - physiology</topic><topic>Humans</topic><topic>Infections</topic><topic>Liver cancer</topic><topic>Male</topic><topic>Medicine and Health Sciences</topic><topic>Messenger RNA</topic><topic>Middle Aged</topic><topic>Mortality</topic><topic>Plasmids</topic><topic>Proteins</topic><topic>Replication</topic><topic>Research and Analysis Methods</topic><topic>Statistical analysis</topic><topic>Virus Replication - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhang, Conghui</au><au>Ma, Qingfeng</au><au>Wang, Wei</au><au>Song, Hui</au><au>Wang, Xue</au><au>Xu, Fengxia</au><au>Zhu, Chengliang</au><au>Liu, Xinghui</au><au>Domfeh, Seth Agyei</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Increased QPCT gene expression by the hepatitis B virus promotes HBV replication</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2024-11-12</date><risdate>2024</risdate><volume>19</volume><issue>11</issue><spage>e0312773</spage><pages>e0312773-</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Glutamine cyclase, an enzyme involved in posttranslational modifications, is encoded by the glutaminyl-peptide cyclotransferase (QPCT) gene. Gene microarray analysis revealed that the QPCT gene was highly expressed in HepG2.2.15 cells compared with that in HepG2 cells. The serum expression level of the QPCT gene was detected by ELISA and was significantly greater in HBV-infected patients than in healthy controls. The mRNA and protein expression levels of the QPCT gene were markedly greater in the HBV-expressing cell lines (HepG2.2.15, and HepG2 and Huh7 cells transfected with the pBlu-HBV plasmid) than in the HepG2 and Huh7 cells. The levels of HBV pgRNA and HBV-DNA copy number, as well as the levels of HBeAg and HBsAg, also increased in the HepG2 and Huh7 cell lines cotransfected with the QPCT gene expression plasmid and the HBV 1.3-fold plasmid. Our study indicated that HBV can promote the expression of the QPCT gene, which in turn promotes the expression and replication of HBV.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>39531472</pmid><doi>10.1371/journal.pone.0312773</doi><tpages>e0312773</tpages><orcidid>https://orcid.org/0000-0002-1676-3235</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Adult Analysis Antigens Bioinformatics Biology and life sciences Cell lines Comparative analysis Copy number Diagnosis DNA microarrays DNA, Viral - genetics Enzyme-linked immunosorbent assay Enzymes Female Gene expression Genes Genetic aspects Genomes Glutamine Glutaminyl-peptide cyclotransferase Health care Hep G2 Cells Hepatitis B Hepatitis B - genetics Hepatitis B - virology Hepatitis B e antigen Hepatitis B e Antigens - genetics Hepatitis B e Antigens - metabolism Hepatitis B surface antigen Hepatitis B Surface Antigens - genetics Hepatitis B Surface Antigens - metabolism Hepatitis B virus - genetics Hepatitis B virus - physiology Humans Infections Liver cancer Male Medicine and Health Sciences Messenger RNA Middle Aged Mortality Plasmids Proteins Replication Research and Analysis Methods Statistical analysis Virus Replication - genetics Viruses |
| title | Increased QPCT gene expression by the hepatitis B virus promotes HBV replication |
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