Evaluation of general anesthesia protocols for a highly controlled cardiac ischemia-reperfusion model in mice
The aim of our study was to test different anesthetic mixtures in order to identify the most suitable one for a surgical cardiac ischemia-reperfusion model in mice. 1) Sixty four mice were submitted to one of the 6 combinations of ketamine or alfaxalone associated to xylazine, medetomidine or midazo...
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| creator | Leon, Christelle Ruelle, Alice Geoffray, Juliette Augeul, Lionel Vogt, Catherine Chiari, Pascal Gomez, Ludovic Ovize, Michel Bidaux, Gabriel Pillot, Bruno |
| description | The aim of our study was to test different anesthetic mixtures in order to identify the most suitable one for a surgical cardiac ischemia-reperfusion model in mice.
1) Sixty four mice were submitted to one of the 6 combinations of ketamine or alfaxalone associated to xylazine, medetomidine or midazolam. Depth and quality of anesthesia were evaluated via 5 reflex scores. 2) Impact of analgesic (buprenorphine or butorphanol), anesthesia reversal (with atipamezole) and surgery (cardiac ischemia-reperfusion surgery) have been tested in the selected protocols. 3) infarction size has been measured with TTC (Triphenyl Tetrazolium Chloride) method in mice anesthetized with best protocols.
Protocol involving medetomidine induced the longest surgical anesthesia: (median = 120, {interquartile range = 100-125}) min with ketamine and 53 {25-100} min with alfaxalone. Butorphanol substitution with buprenorphine did not alter time-related anesthesia parameters. Atipamezole reversal considerably reduced both recovery and immobilization time (respectively 22 {18-30} min and 98 {88-99} min vs. 55 {40-70} min and 143 {131-149} min, in groups with no reversal, p = 0.001) with no impact on infarction size measurement.
In this study, the combination alfaxalone/medetomidine/buprenorphine (80/0,3/0,075 mg.kg-1, s.c.) associated with reversal by atipamezole was a reliable anesthetic protocol for murine surgery, particularly for the study of ischemia-reperfusion. |
| doi_str_mv | 10.1371/journal.pone.0309799 |
| format | Article |
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1) Sixty four mice were submitted to one of the 6 combinations of ketamine or alfaxalone associated to xylazine, medetomidine or midazolam. Depth and quality of anesthesia were evaluated via 5 reflex scores. 2) Impact of analgesic (buprenorphine or butorphanol), anesthesia reversal (with atipamezole) and surgery (cardiac ischemia-reperfusion surgery) have been tested in the selected protocols. 3) infarction size has been measured with TTC (Triphenyl Tetrazolium Chloride) method in mice anesthetized with best protocols.
Protocol involving medetomidine induced the longest surgical anesthesia: (median = 120, {interquartile range = 100-125}) min with ketamine and 53 {25-100} min with alfaxalone. Butorphanol substitution with buprenorphine did not alter time-related anesthesia parameters. Atipamezole reversal considerably reduced both recovery and immobilization time (respectively 22 {18-30} min and 98 {88-99} min vs. 55 {40-70} min and 143 {131-149} min, in groups with no reversal, p = 0.001) with no impact on infarction size measurement.
In this study, the combination alfaxalone/medetomidine/buprenorphine (80/0,3/0,075 mg.kg-1, s.c.) associated with reversal by atipamezole was a reliable anesthetic protocol for murine surgery, particularly for the study of ischemia-reperfusion.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0309799</identifier><identifier>PMID: 39453969</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Analgesics ; Anesthesia ; Anesthesia, General - methods ; Animal models in research ; Animals ; Blood pressure ; Buprenorphine ; Butorphanol ; Care and treatment ; Disease Models, Animal ; General anesthesia ; Heart ; Immobilization ; Infarction ; Ischemia ; Ketamine ; Ketamine - administration & dosage ; Ketamine - pharmacology ; Male ; Medetomidine - administration & dosage ; Medetomidine - pharmacology ; Methods ; Mice ; Midazolam ; Myocardial ischemia ; Myocardial Reperfusion Injury - drug therapy ; Ostomy ; Physiology ; Reperfusion ; Reperfusion injury ; Rodents ; Surgery ; Triphenyltetrazolium chloride ; Ventilators ; Xylazine ; Xylazine - pharmacology</subject><ispartof>PloS one, 2024-10, Vol.19 (10), p.e0309799</ispartof><rights>Copyright: © 2024 Leon et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.</rights><rights>COPYRIGHT 2024 Public Library of Science</rights><rights>2024 Leon et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2024 Leon et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c4018-c307dfc2e7ea84ad01b2a124e9d4b90dee07b913b657f813f3d82bf98439ab5e3</cites><orcidid>0000-0002-1499-3299</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,782,786,866,2932,23875,27933,27934</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/39453969$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Viegas, Carlos Alberto Antunes</contributor><creatorcontrib>Leon, Christelle</creatorcontrib><creatorcontrib>Ruelle, Alice</creatorcontrib><creatorcontrib>Geoffray, Juliette</creatorcontrib><creatorcontrib>Augeul, Lionel</creatorcontrib><creatorcontrib>Vogt, Catherine</creatorcontrib><creatorcontrib>Chiari, Pascal</creatorcontrib><creatorcontrib>Gomez, Ludovic</creatorcontrib><creatorcontrib>Ovize, Michel</creatorcontrib><creatorcontrib>Bidaux, Gabriel</creatorcontrib><creatorcontrib>Pillot, Bruno</creatorcontrib><title>Evaluation of general anesthesia protocols for a highly controlled cardiac ischemia-reperfusion model in mice</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>The aim of our study was to test different anesthetic mixtures in order to identify the most suitable one for a surgical cardiac ischemia-reperfusion model in mice.
1) Sixty four mice were submitted to one of the 6 combinations of ketamine or alfaxalone associated to xylazine, medetomidine or midazolam. Depth and quality of anesthesia were evaluated via 5 reflex scores. 2) Impact of analgesic (buprenorphine or butorphanol), anesthesia reversal (with atipamezole) and surgery (cardiac ischemia-reperfusion surgery) have been tested in the selected protocols. 3) infarction size has been measured with TTC (Triphenyl Tetrazolium Chloride) method in mice anesthetized with best protocols.
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In this study, the combination alfaxalone/medetomidine/buprenorphine (80/0,3/0,075 mg.kg-1, s.c.) associated with reversal by atipamezole was a reliable anesthetic protocol for murine surgery, particularly for the study of ischemia-reperfusion.</description><subject>Analgesics</subject><subject>Anesthesia</subject><subject>Anesthesia, General - methods</subject><subject>Animal models in research</subject><subject>Animals</subject><subject>Blood pressure</subject><subject>Buprenorphine</subject><subject>Butorphanol</subject><subject>Care and treatment</subject><subject>Disease Models, Animal</subject><subject>General anesthesia</subject><subject>Heart</subject><subject>Immobilization</subject><subject>Infarction</subject><subject>Ischemia</subject><subject>Ketamine</subject><subject>Ketamine - administration & dosage</subject><subject>Ketamine - pharmacology</subject><subject>Male</subject><subject>Medetomidine - administration & dosage</subject><subject>Medetomidine - pharmacology</subject><subject>Methods</subject><subject>Mice</subject><subject>Midazolam</subject><subject>Myocardial ischemia</subject><subject>Myocardial Reperfusion Injury - 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Academic</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Leon, Christelle</au><au>Ruelle, Alice</au><au>Geoffray, Juliette</au><au>Augeul, Lionel</au><au>Vogt, Catherine</au><au>Chiari, Pascal</au><au>Gomez, Ludovic</au><au>Ovize, Michel</au><au>Bidaux, Gabriel</au><au>Pillot, Bruno</au><au>Viegas, Carlos Alberto Antunes</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Evaluation of general anesthesia protocols for a highly controlled cardiac ischemia-reperfusion model in mice</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2024-10-25</date><risdate>2024</risdate><volume>19</volume><issue>10</issue><spage>e0309799</spage><pages>e0309799-</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>The aim of our study was to test different anesthetic mixtures in order to identify the most suitable one for a surgical cardiac ischemia-reperfusion model in mice.
1) Sixty four mice were submitted to one of the 6 combinations of ketamine or alfaxalone associated to xylazine, medetomidine or midazolam. Depth and quality of anesthesia were evaluated via 5 reflex scores. 2) Impact of analgesic (buprenorphine or butorphanol), anesthesia reversal (with atipamezole) and surgery (cardiac ischemia-reperfusion surgery) have been tested in the selected protocols. 3) infarction size has been measured with TTC (Triphenyl Tetrazolium Chloride) method in mice anesthetized with best protocols.
Protocol involving medetomidine induced the longest surgical anesthesia: (median = 120, {interquartile range = 100-125}) min with ketamine and 53 {25-100} min with alfaxalone. Butorphanol substitution with buprenorphine did not alter time-related anesthesia parameters. Atipamezole reversal considerably reduced both recovery and immobilization time (respectively 22 {18-30} min and 98 {88-99} min vs. 55 {40-70} min and 143 {131-149} min, in groups with no reversal, p = 0.001) with no impact on infarction size measurement.
In this study, the combination alfaxalone/medetomidine/buprenorphine (80/0,3/0,075 mg.kg-1, s.c.) associated with reversal by atipamezole was a reliable anesthetic protocol for murine surgery, particularly for the study of ischemia-reperfusion.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>39453969</pmid><doi>10.1371/journal.pone.0309799</doi><tpages>e0309799</tpages><orcidid>https://orcid.org/0000-0002-1499-3299</orcidid><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; DOAJ Directory of Open Access Journals; Public Library of Science (PLoS) Journals Open Access; EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Analgesics Anesthesia Anesthesia, General - methods Animal models in research Animals Blood pressure Buprenorphine Butorphanol Care and treatment Disease Models, Animal General anesthesia Heart Immobilization Infarction Ischemia Ketamine Ketamine - administration & dosage Ketamine - pharmacology Male Medetomidine - administration & dosage Medetomidine - pharmacology Methods Mice Midazolam Myocardial ischemia Myocardial Reperfusion Injury - drug therapy Ostomy Physiology Reperfusion Reperfusion injury Rodents Surgery Triphenyltetrazolium chloride Ventilators Xylazine Xylazine - pharmacology |
| title | Evaluation of general anesthesia protocols for a highly controlled cardiac ischemia-reperfusion model in mice |
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