Effect of type of anticoagulant, transportation time, and glucose in the culture media on neutrophil viability and function test results in dairy cattle

In dairy cattle research, in vitro assessment of innate immune function is commonly evaluated by flow cytometry via the quantitative analysis of circulating polymorphonuclear leukocytes (PMN) functionalities specifically focusing on the capacities for phagocytosis (PC) and oxidative burst (OB). Vari...

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Veröffentlicht in:	PloS one 2024-10, Vol.19 (10), p.e0311742
Hauptverfasser:	Chandrappa, Sanjana Malledevarahalli, Xie, Lei, Andueza, Sebastian Gonzalez, Sadeghi, Hafez, Rashid, Muhammad Hussnain, Niazi, Mehrnaz, Qiao, Kaixi, Dong, Qiang, Vincenti, Leila, Ricci, Alessandro, Pascottini, Osvaldo Bogado, Opsomer, Geert
Format:	Artikel
Sprache:	eng
Schlagworte:	Acetic acid Acids Analysis Animals Anticoagulants Anticoagulants (Medicine) Anticoagulants - pharmacology Apoptosis Biology and Life Sciences Blood Cattle Cell culture Cell Survival - drug effects Centrifugation Citric Acid Culture Media Dairy cattle Diseases Dosage and administration Edetic acid Edetic Acid - pharmacology Engineering and Technology Ethylenediaminetetraacetic acids Female Flow cytometry Fluorescence Genetic aspects Glucose Glucose - analogs & derivatives Glucose - metabolism Growth Immune response In vitro methods and tests In vivo methods and tests Leukocytes (neutrophilic) Leukocytes (polymorphonuclear) Medical examination Medicine and Health Sciences Neutrophils - cytology Neutrophils - drug effects Neutrophils - metabolism Parameters Phagocytosis Phagocytosis - drug effects Phorbol 12-myristate 13-acetate Physical Sciences Regression analysis Regression models Research and Analysis Methods Respiratory Burst - drug effects Sample size Time Factors Transportation Tubes Viability
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