In vitro co-culture system for investigating Armillaria root rot in Prunus spp. using a fiber-supported liquid approach
In vitro co-culture techniques that allow the growth of plants and pathogens under controlled environmental conditions are being used to re-create host plant infection. These approaches reduce infection times, promote reproducibility, and enable a rapid evaluation of plant-pathogen interactions. As...
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| Veröffentlicht in: | PloS one 2024-09, Vol.19 (9), p.e0310314 |
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| creator | Calle, Alejandro Adelberg, Jeffrey Schnabel, Guido Naylor-Adelberg, Jacqueline Gelain, Jhulia Karakoc, Yeter Weaver, Jared Saski, Christopher Gasic, Ksenija |
| description | In vitro co-culture techniques that allow the growth of plants and pathogens under controlled environmental conditions are being used to re-create host plant infection. These approaches reduce infection times, promote reproducibility, and enable a rapid evaluation of plant-pathogen interactions. As a result, these systems have become essential in breeding programs aimed at developing plant resistance to diseases. In this study, we developed and validated an in vitro co-culture system to investigate the Armillaria root rot (ARR) affecting Prunus spp. This disease, caused by fungi Armillaria spp. and Desarmillaria caespitosa, poses a severe threat to the stone and nut fruit industry due to the susceptibility of most commercial rootstocks to infection and the lack of effective management options for its control. The system consists of a fiber-supported liquid approach in sterile plastic vessels that allows a fast and reproducible fungal infection under controlled environmental conditions. The floor of the vessels was covered with a polyester-fiber matte and a germination paper that served as an interface between the mycelia and the plant roots. The vessels were subjected to inoculation with Armillaria mellea and D. caespitosa, and three Prunus genotypes ('Guardian®', 'MP-29', and Prunus cerasifera '14-4') were co-cultured with both fungi. Disease progression and plant and fungal biomass were monitored during co-culture. The presented in vitro co-culture approach facilitates the concurrent growth of Armillaria/Desarmillaria spp. and Prunus spp., excluding most of the limitations associated with greenhouses and field experiments. This system provides consistent and reproducible conditions for investigating a prominent plant disease affecting Prunus spp. |
| doi_str_mv | 10.1371/journal.pone.0310314 |
| format | Article |
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These approaches reduce infection times, promote reproducibility, and enable a rapid evaluation of plant-pathogen interactions. As a result, these systems have become essential in breeding programs aimed at developing plant resistance to diseases. In this study, we developed and validated an in vitro co-culture system to investigate the Armillaria root rot (ARR) affecting Prunus spp. This disease, caused by fungi Armillaria spp. and Desarmillaria caespitosa, poses a severe threat to the stone and nut fruit industry due to the susceptibility of most commercial rootstocks to infection and the lack of effective management options for its control. The system consists of a fiber-supported liquid approach in sterile plastic vessels that allows a fast and reproducible fungal infection under controlled environmental conditions. The floor of the vessels was covered with a polyester-fiber matte and a germination paper that served as an interface between the mycelia and the plant roots. The vessels were subjected to inoculation with Armillaria mellea and D. caespitosa, and three Prunus genotypes ('Guardian®', 'MP-29', and Prunus cerasifera '14-4') were co-cultured with both fungi. Disease progression and plant and fungal biomass were monitored during co-culture. The presented in vitro co-culture approach facilitates the concurrent growth of Armillaria/Desarmillaria spp. and Prunus spp., excluding most of the limitations associated with greenhouses and field experiments. This system provides consistent and reproducible conditions for investigating a prominent plant disease affecting Prunus spp.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0310314</identifier><identifier>PMID: 39312543</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Analysis ; Antifungal agents ; Armillaria ; Armillaria - genetics ; Biology and Life Sciences ; Breeding ; Coculture Techniques - methods ; Control ; Culture techniques ; Disease control ; Disease susceptibility ; Dosage and administration ; Environmental conditions ; Environmental management ; Field tests ; Fruit industry ; Fungi ; Genetic aspects ; Genotypes ; Germination ; Health aspects ; Host plants ; Identification and classification ; Infection ; Infection control ; Infections ; Inoculation ; Lab Protocol ; Laboratories ; Medical research ; Medicine and Health Sciences ; Medicine, Experimental ; Methods ; Pathogens ; Physical Sciences ; Plant breeding ; Plant diseases ; Plant Diseases - microbiology ; Plant growth ; Plant layout ; Plant resistance ; Plant roots ; Plant Roots - growth & development ; Plant Roots - microbiology ; Protocol ; Prunus ; Prunus - microbiology ; Reproducibility ; Root rot ; Seeds ; Vessels</subject><ispartof>PloS one, 2024-09, Vol.19 (9), p.e0310314</ispartof><rights>Copyright: © 2024 Calle et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.</rights><rights>COPYRIGHT 2024 Public Library of Science</rights><rights>2024 Calle et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2024 Calle et al 2024 Calle et al</rights><rights>2024 Calle et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c460t-2d03b9f90fbbb7ee19f903d840a6a1a132a8630f0a00a354bed6b2e5c0c572f83</cites><orcidid>0000-0003-4391-5262</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC11419352/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC11419352/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,2914,23846,27903,27904,53769,53771,79346,79347</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/39312543$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Pandey, Abhay K.</contributor><creatorcontrib>Calle, Alejandro</creatorcontrib><creatorcontrib>Adelberg, Jeffrey</creatorcontrib><creatorcontrib>Schnabel, Guido</creatorcontrib><creatorcontrib>Naylor-Adelberg, Jacqueline</creatorcontrib><creatorcontrib>Gelain, Jhulia</creatorcontrib><creatorcontrib>Karakoc, Yeter</creatorcontrib><creatorcontrib>Weaver, Jared</creatorcontrib><creatorcontrib>Saski, Christopher</creatorcontrib><creatorcontrib>Gasic, Ksenija</creatorcontrib><title>In vitro co-culture system for investigating Armillaria root rot in Prunus spp. using a fiber-supported liquid approach</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>In vitro co-culture techniques that allow the growth of plants and pathogens under controlled environmental conditions are being used to re-create host plant infection. 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These approaches reduce infection times, promote reproducibility, and enable a rapid evaluation of plant-pathogen interactions. As a result, these systems have become essential in breeding programs aimed at developing plant resistance to diseases. In this study, we developed and validated an in vitro co-culture system to investigate the Armillaria root rot (ARR) affecting Prunus spp. This disease, caused by fungi Armillaria spp. and Desarmillaria caespitosa, poses a severe threat to the stone and nut fruit industry due to the susceptibility of most commercial rootstocks to infection and the lack of effective management options for its control. The system consists of a fiber-supported liquid approach in sterile plastic vessels that allows a fast and reproducible fungal infection under controlled environmental conditions. The floor of the vessels was covered with a polyester-fiber matte and a germination paper that served as an interface between the mycelia and the plant roots. The vessels were subjected to inoculation with Armillaria mellea and D. caespitosa, and three Prunus genotypes ('Guardian®', 'MP-29', and Prunus cerasifera '14-4') were co-cultured with both fungi. Disease progression and plant and fungal biomass were monitored during co-culture. The presented in vitro co-culture approach facilitates the concurrent growth of Armillaria/Desarmillaria spp. and Prunus spp., excluding most of the limitations associated with greenhouses and field experiments. This system provides consistent and reproducible conditions for investigating a prominent plant disease affecting Prunus spp.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>39312543</pmid><doi>10.1371/journal.pone.0310314</doi><tpages>e0310314</tpages><orcidid>https://orcid.org/0000-0003-4391-5262</orcidid><oa>free_for_read</oa></addata></record> |
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| ispartof | PloS one, 2024-09, Vol.19 (9), p.e0310314 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_3108572914 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Public Library of Science (PLoS) Journals Open Access; EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Analysis Antifungal agents Armillaria Armillaria - genetics Biology and Life Sciences Breeding Coculture Techniques - methods Control Culture techniques Disease control Disease susceptibility Dosage and administration Environmental conditions Environmental management Field tests Fruit industry Fungi Genetic aspects Genotypes Germination Health aspects Host plants Identification and classification Infection Infection control Infections Inoculation Lab Protocol Laboratories Medical research Medicine and Health Sciences Medicine, Experimental Methods Pathogens Physical Sciences Plant breeding Plant diseases Plant Diseases - microbiology Plant growth Plant layout Plant resistance Plant roots Plant Roots - growth & development Plant Roots - microbiology Protocol Prunus Prunus - microbiology Reproducibility Root rot Seeds Vessels |
| title | In vitro co-culture system for investigating Armillaria root rot in Prunus spp. using a fiber-supported liquid approach |
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