Cell migration and proliferation capacity of IPEC-J2 cells after short-chain fatty acid exposure
Novel antimicrobial strategies are necessary to tackle using antibiotics during the suckling and weaning period of piglets, often characterized by E. coli-induced diarrhea. In the last decades, acetate, propionate, and butyrate, all short-chain fatty acids (SCFAs), have been proposed as an alternati...
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| description | Novel antimicrobial strategies are necessary to tackle using antibiotics during the suckling and weaning period of piglets, often characterized by E. coli-induced diarrhea. In the last decades, acetate, propionate, and butyrate, all short-chain fatty acids (SCFAs), have been proposed as an alternative to antibiotics. SCFAs are instrumental in promoting the proliferation of enterocytes, preserving intestinal integrity, and modulating the microbial community by suppressing the growth of pathogenic bacteria in pigs. The effect of individual SCFAs (proprionate, acetate and butyrate) on the regenerative capacity of intestinal cells was investigated via an optimized wound-healing assay in IPEC-J2 cells, a porcine jejunal epithelial cell line. IPEC-J2 cells proved a good model as they express the free fatty acid receptor 2 (FFAR2), an important SCFA receptor with a high affinity for proprionate. Our study demonstrated that propionate (p = 0.005) and acetate (p = 0.037) were more effective in closing the wound than butyrate (p = 0.190). This holds promise in using SCFA's per os as an alternative to antibiotics. |
| doi_str_mv | 10.1371/journal.pone.0309742 |
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In the last decades, acetate, propionate, and butyrate, all short-chain fatty acids (SCFAs), have been proposed as an alternative to antibiotics. SCFAs are instrumental in promoting the proliferation of enterocytes, preserving intestinal integrity, and modulating the microbial community by suppressing the growth of pathogenic bacteria in pigs. The effect of individual SCFAs (proprionate, acetate and butyrate) on the regenerative capacity of intestinal cells was investigated via an optimized wound-healing assay in IPEC-J2 cells, a porcine jejunal epithelial cell line. IPEC-J2 cells proved a good model as they express the free fatty acid receptor 2 (FFAR2), an important SCFA receptor with a high affinity for proprionate. Our study demonstrated that propionate (p = 0.005) and acetate (p = 0.037) were more effective in closing the wound than butyrate (p = 0.190). This holds promise in using SCFA's per os as an alternative to antibiotics.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0309742</identifier><identifier>PMID: 39213333</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Acetic acid ; Animals ; Antibiotics ; Antimicrobial agents ; Butyrates - pharmacology ; Cell growth ; Cell Line ; Cell lines ; Cell migration ; Cell Movement - drug effects ; Cell proliferation ; Cell Proliferation - drug effects ; Diarrhea ; E coli ; Enterocytes ; Epidermal growth factor ; Epithelial cells ; Epithelial Cells - drug effects ; Epithelial Cells - metabolism ; Epithelium ; Experiments ; Fatty acids ; Fatty Acids, Volatile - metabolism ; Fatty Acids, Volatile - pharmacology ; Hogs ; Immunohistochemistry ; Intestinal Mucosa - cytology ; Intestinal Mucosa - drug effects ; Intestinal Mucosa - metabolism ; Intestinal Mucosa - microbiology ; Intestine ; Large intestine ; Microorganisms ; Penicillin ; Physiological aspects ; Propionates - pharmacology ; Propionic acid ; Receptors ; Small intestine ; Suckling behavior ; Swine ; Weaning ; Wound healing</subject><ispartof>PloS one, 2024-08, Vol.19 (8), p.e0309742</ispartof><rights>Copyright: © 2024 Van Bockstal et al. 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In the last decades, acetate, propionate, and butyrate, all short-chain fatty acids (SCFAs), have been proposed as an alternative to antibiotics. SCFAs are instrumental in promoting the proliferation of enterocytes, preserving intestinal integrity, and modulating the microbial community by suppressing the growth of pathogenic bacteria in pigs. The effect of individual SCFAs (proprionate, acetate and butyrate) on the regenerative capacity of intestinal cells was investigated via an optimized wound-healing assay in IPEC-J2 cells, a porcine jejunal epithelial cell line. IPEC-J2 cells proved a good model as they express the free fatty acid receptor 2 (FFAR2), an important SCFA receptor with a high affinity for proprionate. Our study demonstrated that propionate (p = 0.005) and acetate (p = 0.037) were more effective in closing the wound than butyrate (p = 0.190). This holds promise in using SCFA's per os as an alternative to antibiotics.</description><subject>Acetic acid</subject><subject>Animals</subject><subject>Antibiotics</subject><subject>Antimicrobial agents</subject><subject>Butyrates - pharmacology</subject><subject>Cell growth</subject><subject>Cell Line</subject><subject>Cell lines</subject><subject>Cell migration</subject><subject>Cell Movement - drug effects</subject><subject>Cell proliferation</subject><subject>Cell Proliferation - drug effects</subject><subject>Diarrhea</subject><subject>E coli</subject><subject>Enterocytes</subject><subject>Epidermal growth factor</subject><subject>Epithelial cells</subject><subject>Epithelial Cells - drug effects</subject><subject>Epithelial Cells - metabolism</subject><subject>Epithelium</subject><subject>Experiments</subject><subject>Fatty acids</subject><subject>Fatty Acids, Volatile - metabolism</subject><subject>Fatty Acids, Volatile - pharmacology</subject><subject>Hogs</subject><subject>Immunohistochemistry</subject><subject>Intestinal Mucosa - 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| subjects | Acetic acid Animals Antibiotics Antimicrobial agents Butyrates - pharmacology Cell growth Cell Line Cell lines Cell migration Cell Movement - drug effects Cell proliferation Cell Proliferation - drug effects Diarrhea E coli Enterocytes Epidermal growth factor Epithelial cells Epithelial Cells - drug effects Epithelial Cells - metabolism Epithelium Experiments Fatty acids Fatty Acids, Volatile - metabolism Fatty Acids, Volatile - pharmacology Hogs Immunohistochemistry Intestinal Mucosa - cytology Intestinal Mucosa - drug effects Intestinal Mucosa - metabolism Intestinal Mucosa - microbiology Intestine Large intestine Microorganisms Penicillin Physiological aspects Propionates - pharmacology Propionic acid Receptors Small intestine Suckling behavior Swine Weaning Wound healing |
| title | Cell migration and proliferation capacity of IPEC-J2 cells after short-chain fatty acid exposure |
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