Emergence of crucial evidence catalyzing the origin tracing of SARS-CoV-2
Since the emergence of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), its genetic and geographical origins remain unclear, resulting in suspicions about its natural origin. In one of our previous studies, we reported the presence of a furin cleavage site RRAR in the junction region be...
Gespeichert in:
| Veröffentlicht in: | PloS one 2024-08, Vol.19 (8), p.e0309557 |
|---|---|
| Hauptverfasser: | , , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | |
|---|---|
| container_issue | 8 |
| container_start_page | e0309557 |
| container_title | PloS one |
| container_volume | 19 |
| creator | Chen, Shunmei Ruan, Cihan Guo, Yutong Chang, Jia Yan, Haohao Chen, Liang Duan, Yongzhong Duan, Guangyou Bei, Jinlong Li, Xin Gao, Shan |
| description | Since the emergence of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), its genetic and geographical origins remain unclear, resulting in suspicions about its natural origin. In one of our previous studies, we reported the presence of a furin cleavage site RRAR in the junction region between S1 and S2 subunits of the spike protein, which was discovered as the first crucial clue for the origin tracing of SARS-CoV-2. In the present study, we conducted an integrative analysis of new genome data from bat Sarbecovirus strains reported after the COVID-19 outbreak. The primary results included the identification of BANAL-20-52, Rp22DB159, and S18CXBatR24 as three close relatives of SARS-CoV-2 and the successful detection of seven out of nine key genomic features (designated as RC0-7 and ORF8) observed in wild types of SARS-CoV-2 in the three close relatives from Laos, Vietnam, and Yunnan province of China, respectively. The most significant contribution of the present study lies in the detection of RC1 in wild genotype in a bat Sarbecovirus population BANAL-20-52 belonging to. Encoding a segment of the NSP3 protein, RC1 was discovered as the second crucial clue for the origin tracing of SARS-CoV-2. Although RC0, encoding the junction furin cleavage site, remains undetected outside of the SARS-CoV-2 genome, Feuang of Laos is the sole place where eight of the nine wild-type features (RC1-7 and ORF8) have been detected. |
| doi_str_mv | 10.1371/journal.pone.0309557 |
| format | Article |
| fullrecord | <record><control><sourceid>gale_plos_</sourceid><recordid>TN_cdi_plos_journals_3099096979</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A806664708</galeid><doaj_id>oai_doaj_org_article_a1c60eaf0c594165b69a7ca6d930d513</doaj_id><sourcerecordid>A806664708</sourcerecordid><originalsourceid>FETCH-LOGICAL-c516t-7433ce11ab2744f9c4a7ee204a3900dcbe6d5ced9b2dce9d4e512abf8984e2733</originalsourceid><addsrcrecordid>eNqNklFr2zAUhc3YWLts_2BsgcLYHpxJli1ZjyF0W6BQaLa-imvp2lFwrE6yx7pfP7lxSzP6MPQgcfiOjji6SfKWkgVlgn7eucF30C5uXIcLwogsCvEsOaWSZSnPCHv-6HySvAphR0jBSs5fJidMZpRlUpwm6_M9-gY7jXNXz7UftIV2jr-sudM09NDe_rFdM--3EfG2sd2896BHKTo2y6tNunLXafY6eVFDG_DNtM-SH1_Ov6--pReXX9er5UWqC8r7VOSMaaQUqkzkeS11DgIxIzkwSYjRFXJTaDSyyoxGaXIsaAZVXcoyx0wwNkveH-69aV1QUwtBxQIkkVwKGYn1gTAOdurG2z34W-XAqjvB-UaB761uUQHVnCDURBcyp7youAShgRvJiCnomPZxSvPu54ChV3sbNLYtdOiGQ2xJchHZWXL2D_r04yaqgZhvu9qNbY6XqmVJOOe5IGWkFk9QcRncWx1_vLZRPzJ8OjJEpsfffQNDCGq9ufp_9vL6mP3wiN0itP02uHborevCMZgfQO1dCB7rh-IpUePA3rehxoFV08BG27uptKHao3kw3U8o-ws5XeLK</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>3099096979</pqid></control><display><type>article</type><title>Emergence of crucial evidence catalyzing the origin tracing of SARS-CoV-2</title><source>MEDLINE</source><source>DOAJ Directory of Open Access Journals</source><source>Public Library of Science (PLoS)</source><source>EZB-FREE-00999 freely available EZB journals</source><source>PubMed Central</source><source>Free Full-Text Journals in Chemistry</source><creator>Chen, Shunmei ; Ruan, Cihan ; Guo, Yutong ; Chang, Jia ; Yan, Haohao ; Chen, Liang ; Duan, Yongzhong ; Duan, Guangyou ; Bei, Jinlong ; Li, Xin ; Gao, Shan</creator><contributor>Anjorin, AbdulAzeez Adeyemi</contributor><creatorcontrib>Chen, Shunmei ; Ruan, Cihan ; Guo, Yutong ; Chang, Jia ; Yan, Haohao ; Chen, Liang ; Duan, Yongzhong ; Duan, Guangyou ; Bei, Jinlong ; Li, Xin ; Gao, Shan ; Anjorin, AbdulAzeez Adeyemi</creatorcontrib><description>Since the emergence of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), its genetic and geographical origins remain unclear, resulting in suspicions about its natural origin. In one of our previous studies, we reported the presence of a furin cleavage site RRAR in the junction region between S1 and S2 subunits of the spike protein, which was discovered as the first crucial clue for the origin tracing of SARS-CoV-2. In the present study, we conducted an integrative analysis of new genome data from bat Sarbecovirus strains reported after the COVID-19 outbreak. The primary results included the identification of BANAL-20-52, Rp22DB159, and S18CXBatR24 as three close relatives of SARS-CoV-2 and the successful detection of seven out of nine key genomic features (designated as RC0-7 and ORF8) observed in wild types of SARS-CoV-2 in the three close relatives from Laos, Vietnam, and Yunnan province of China, respectively. The most significant contribution of the present study lies in the detection of RC1 in wild genotype in a bat Sarbecovirus population BANAL-20-52 belonging to. Encoding a segment of the NSP3 protein, RC1 was discovered as the second crucial clue for the origin tracing of SARS-CoV-2. Although RC0, encoding the junction furin cleavage site, remains undetected outside of the SARS-CoV-2 genome, Feuang of Laos is the sole place where eight of the nine wild-type features (RC1-7 and ORF8) have been detected.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0309557</identifier><identifier>PMID: 39213297</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Animals ; China - epidemiology ; Chiroptera - virology ; Cleavage ; Coronaviruses ; COVID-19 ; COVID-19 - epidemiology ; COVID-19 - virology ; Disease transmission ; Furin ; Furin - genetics ; Furin - metabolism ; Genome, Viral ; Genomes ; Genomic analysis ; Genotypes ; Geographical distribution ; Humans ; Hypotheses ; Mutation ; NSP3 protein ; Peptides ; Phylogenetics ; Phylogeny ; Population studies ; Proteins ; Sarbecovirus ; SARS-CoV-2 - genetics ; SARS-CoV-2 - isolation & purification ; Severe acute respiratory syndrome coronavirus 2 ; Software ; Spike Glycoprotein, Coronavirus - genetics ; Spike protein ; Tracing ; Viral diseases ; Virus research</subject><ispartof>PloS one, 2024-08, Vol.19 (8), p.e0309557</ispartof><rights>Copyright: © 2024 Chen et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.</rights><rights>COPYRIGHT 2024 Public Library of Science</rights><rights>2024 Chen et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2024 Chen et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c516t-7433ce11ab2744f9c4a7ee204a3900dcbe6d5ced9b2dce9d4e512abf8984e2733</cites><orcidid>0000-0001-5502-7794 ; 0000-0002-8919-1338</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0309557&type=printable$$EPDF$$P50$$Gplos$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://journals.plos.org/plosone/article?id=10.1371/journal.pone.0309557$$EHTML$$P50$$Gplos$$Hfree_for_read</linktohtml><link.rule.ids>314,776,780,860,2095,2914,23846,27903,27904,79346,79347</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/39213297$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Anjorin, AbdulAzeez Adeyemi</contributor><creatorcontrib>Chen, Shunmei</creatorcontrib><creatorcontrib>Ruan, Cihan</creatorcontrib><creatorcontrib>Guo, Yutong</creatorcontrib><creatorcontrib>Chang, Jia</creatorcontrib><creatorcontrib>Yan, Haohao</creatorcontrib><creatorcontrib>Chen, Liang</creatorcontrib><creatorcontrib>Duan, Yongzhong</creatorcontrib><creatorcontrib>Duan, Guangyou</creatorcontrib><creatorcontrib>Bei, Jinlong</creatorcontrib><creatorcontrib>Li, Xin</creatorcontrib><creatorcontrib>Gao, Shan</creatorcontrib><title>Emergence of crucial evidence catalyzing the origin tracing of SARS-CoV-2</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Since the emergence of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), its genetic and geographical origins remain unclear, resulting in suspicions about its natural origin. In one of our previous studies, we reported the presence of a furin cleavage site RRAR in the junction region between S1 and S2 subunits of the spike protein, which was discovered as the first crucial clue for the origin tracing of SARS-CoV-2. In the present study, we conducted an integrative analysis of new genome data from bat Sarbecovirus strains reported after the COVID-19 outbreak. The primary results included the identification of BANAL-20-52, Rp22DB159, and S18CXBatR24 as three close relatives of SARS-CoV-2 and the successful detection of seven out of nine key genomic features (designated as RC0-7 and ORF8) observed in wild types of SARS-CoV-2 in the three close relatives from Laos, Vietnam, and Yunnan province of China, respectively. The most significant contribution of the present study lies in the detection of RC1 in wild genotype in a bat Sarbecovirus population BANAL-20-52 belonging to. Encoding a segment of the NSP3 protein, RC1 was discovered as the second crucial clue for the origin tracing of SARS-CoV-2. Although RC0, encoding the junction furin cleavage site, remains undetected outside of the SARS-CoV-2 genome, Feuang of Laos is the sole place where eight of the nine wild-type features (RC1-7 and ORF8) have been detected.</description><subject>Animals</subject><subject>China - epidemiology</subject><subject>Chiroptera - virology</subject><subject>Cleavage</subject><subject>Coronaviruses</subject><subject>COVID-19</subject><subject>COVID-19 - epidemiology</subject><subject>COVID-19 - virology</subject><subject>Disease transmission</subject><subject>Furin</subject><subject>Furin - genetics</subject><subject>Furin - metabolism</subject><subject>Genome, Viral</subject><subject>Genomes</subject><subject>Genomic analysis</subject><subject>Genotypes</subject><subject>Geographical distribution</subject><subject>Humans</subject><subject>Hypotheses</subject><subject>Mutation</subject><subject>NSP3 protein</subject><subject>Peptides</subject><subject>Phylogenetics</subject><subject>Phylogeny</subject><subject>Population studies</subject><subject>Proteins</subject><subject>Sarbecovirus</subject><subject>SARS-CoV-2 - genetics</subject><subject>SARS-CoV-2 - isolation & purification</subject><subject>Severe acute respiratory syndrome coronavirus 2</subject><subject>Software</subject><subject>Spike Glycoprotein, Coronavirus - genetics</subject><subject>Spike protein</subject><subject>Tracing</subject><subject>Viral diseases</subject><subject>Virus research</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>DOA</sourceid><recordid>eNqNklFr2zAUhc3YWLts_2BsgcLYHpxJli1ZjyF0W6BQaLa-imvp2lFwrE6yx7pfP7lxSzP6MPQgcfiOjji6SfKWkgVlgn7eucF30C5uXIcLwogsCvEsOaWSZSnPCHv-6HySvAphR0jBSs5fJidMZpRlUpwm6_M9-gY7jXNXz7UftIV2jr-sudM09NDe_rFdM--3EfG2sd2896BHKTo2y6tNunLXafY6eVFDG_DNtM-SH1_Ov6--pReXX9er5UWqC8r7VOSMaaQUqkzkeS11DgIxIzkwSYjRFXJTaDSyyoxGaXIsaAZVXcoyx0wwNkveH-69aV1QUwtBxQIkkVwKGYn1gTAOdurG2z34W-XAqjvB-UaB761uUQHVnCDURBcyp7youAShgRvJiCnomPZxSvPu54ChV3sbNLYtdOiGQ2xJchHZWXL2D_r04yaqgZhvu9qNbY6XqmVJOOe5IGWkFk9QcRncWx1_vLZRPzJ8OjJEpsfffQNDCGq9ufp_9vL6mP3wiN0itP02uHborevCMZgfQO1dCB7rh-IpUePA3rehxoFV08BG27uptKHao3kw3U8o-ws5XeLK</recordid><startdate>20240830</startdate><enddate>20240830</enddate><creator>Chen, Shunmei</creator><creator>Ruan, Cihan</creator><creator>Guo, Yutong</creator><creator>Chang, Jia</creator><creator>Yan, Haohao</creator><creator>Chen, Liang</creator><creator>Duan, Yongzhong</creator><creator>Duan, Guangyou</creator><creator>Bei, Jinlong</creator><creator>Li, Xin</creator><creator>Gao, Shan</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>COVID</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0001-5502-7794</orcidid><orcidid>https://orcid.org/0000-0002-8919-1338</orcidid></search><sort><creationdate>20240830</creationdate><title>Emergence of crucial evidence catalyzing the origin tracing of SARS-CoV-2</title><author>Chen, Shunmei ; Ruan, Cihan ; Guo, Yutong ; Chang, Jia ; Yan, Haohao ; Chen, Liang ; Duan, Yongzhong ; Duan, Guangyou ; Bei, Jinlong ; Li, Xin ; Gao, Shan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c516t-7433ce11ab2744f9c4a7ee204a3900dcbe6d5ced9b2dce9d4e512abf8984e2733</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Animals</topic><topic>China - epidemiology</topic><topic>Chiroptera - virology</topic><topic>Cleavage</topic><topic>Coronaviruses</topic><topic>COVID-19</topic><topic>COVID-19 - epidemiology</topic><topic>COVID-19 - virology</topic><topic>Disease transmission</topic><topic>Furin</topic><topic>Furin - genetics</topic><topic>Furin - metabolism</topic><topic>Genome, Viral</topic><topic>Genomes</topic><topic>Genomic analysis</topic><topic>Genotypes</topic><topic>Geographical distribution</topic><topic>Humans</topic><topic>Hypotheses</topic><topic>Mutation</topic><topic>NSP3 protein</topic><topic>Peptides</topic><topic>Phylogenetics</topic><topic>Phylogeny</topic><topic>Population studies</topic><topic>Proteins</topic><topic>Sarbecovirus</topic><topic>SARS-CoV-2 - genetics</topic><topic>SARS-CoV-2 - isolation & purification</topic><topic>Severe acute respiratory syndrome coronavirus 2</topic><topic>Software</topic><topic>Spike Glycoprotein, Coronavirus - genetics</topic><topic>Spike protein</topic><topic>Tracing</topic><topic>Viral diseases</topic><topic>Virus research</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chen, Shunmei</creatorcontrib><creatorcontrib>Ruan, Cihan</creatorcontrib><creatorcontrib>Guo, Yutong</creatorcontrib><creatorcontrib>Chang, Jia</creatorcontrib><creatorcontrib>Yan, Haohao</creatorcontrib><creatorcontrib>Chen, Liang</creatorcontrib><creatorcontrib>Duan, Yongzhong</creatorcontrib><creatorcontrib>Duan, Guangyou</creatorcontrib><creatorcontrib>Bei, Jinlong</creatorcontrib><creatorcontrib>Li, Xin</creatorcontrib><creatorcontrib>Gao, Shan</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: Opposing Viewpoints</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Nursing & Allied Health Database</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Meteorological & Geoastrophysical Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>Coronavirus Research Database</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Meteorological & Geoastrophysical Abstracts - Academic</collection><collection>ProQuest Engineering Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Engineering Database</collection><collection>Nursing & Allied Health Premium</collection><collection>Advanced Technologies & Aerospace Database</collection><collection>ProQuest Advanced Technologies & Aerospace Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>Materials Science Collection</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Engineering Collection</collection><collection>Environmental Science Collection</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chen, Shunmei</au><au>Ruan, Cihan</au><au>Guo, Yutong</au><au>Chang, Jia</au><au>Yan, Haohao</au><au>Chen, Liang</au><au>Duan, Yongzhong</au><au>Duan, Guangyou</au><au>Bei, Jinlong</au><au>Li, Xin</au><au>Gao, Shan</au><au>Anjorin, AbdulAzeez Adeyemi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Emergence of crucial evidence catalyzing the origin tracing of SARS-CoV-2</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2024-08-30</date><risdate>2024</risdate><volume>19</volume><issue>8</issue><spage>e0309557</spage><pages>e0309557-</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Since the emergence of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), its genetic and geographical origins remain unclear, resulting in suspicions about its natural origin. In one of our previous studies, we reported the presence of a furin cleavage site RRAR in the junction region between S1 and S2 subunits of the spike protein, which was discovered as the first crucial clue for the origin tracing of SARS-CoV-2. In the present study, we conducted an integrative analysis of new genome data from bat Sarbecovirus strains reported after the COVID-19 outbreak. The primary results included the identification of BANAL-20-52, Rp22DB159, and S18CXBatR24 as three close relatives of SARS-CoV-2 and the successful detection of seven out of nine key genomic features (designated as RC0-7 and ORF8) observed in wild types of SARS-CoV-2 in the three close relatives from Laos, Vietnam, and Yunnan province of China, respectively. The most significant contribution of the present study lies in the detection of RC1 in wild genotype in a bat Sarbecovirus population BANAL-20-52 belonging to. Encoding a segment of the NSP3 protein, RC1 was discovered as the second crucial clue for the origin tracing of SARS-CoV-2. Although RC0, encoding the junction furin cleavage site, remains undetected outside of the SARS-CoV-2 genome, Feuang of Laos is the sole place where eight of the nine wild-type features (RC1-7 and ORF8) have been detected.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>39213297</pmid><doi>10.1371/journal.pone.0309557</doi><tpages>e0309557</tpages><orcidid>https://orcid.org/0000-0001-5502-7794</orcidid><orcidid>https://orcid.org/0000-0002-8919-1338</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2024-08, Vol.19 (8), p.e0309557 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_3099096979 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Public Library of Science (PLoS); EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Animals China - epidemiology Chiroptera - virology Cleavage Coronaviruses COVID-19 COVID-19 - epidemiology COVID-19 - virology Disease transmission Furin Furin - genetics Furin - metabolism Genome, Viral Genomes Genomic analysis Genotypes Geographical distribution Humans Hypotheses Mutation NSP3 protein Peptides Phylogenetics Phylogeny Population studies Proteins Sarbecovirus SARS-CoV-2 - genetics SARS-CoV-2 - isolation & purification Severe acute respiratory syndrome coronavirus 2 Software Spike Glycoprotein, Coronavirus - genetics Spike protein Tracing Viral diseases Virus research |
| title | Emergence of crucial evidence catalyzing the origin tracing of SARS-CoV-2 |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-26T07%3A24%3A36IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Emergence%20of%20crucial%20evidence%20catalyzing%20the%20origin%20tracing%20of%20SARS-CoV-2&rft.jtitle=PloS%20one&rft.au=Chen,%20Shunmei&rft.date=2024-08-30&rft.volume=19&rft.issue=8&rft.spage=e0309557&rft.pages=e0309557-&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0309557&rft_dat=%3Cgale_plos_%3EA806664708%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=3099096979&rft_id=info:pmid/39213297&rft_galeid=A806664708&rft_doaj_id=oai_doaj_org_article_a1c60eaf0c594165b69a7ca6d930d513&rfr_iscdi=true |