Naturally acquired antibodies against 4 Streptococcus pneumoniae serotypes in Pakistani adults with type 2 diabetes mellitus
Immune response elicited during pneumococcal carriage has been shown to protect against subsequent colonization and infection by Streptococcus pneumoniae. The study was designed to measure the baseline serotype-specific anti-capsular IgG concentration and opsonic titers elicited in response to asymp...
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| description | Immune response elicited during pneumococcal carriage has been shown to protect against subsequent colonization and infection by Streptococcus pneumoniae. The study was designed to measure the baseline serotype-specific anti-capsular IgG concentration and opsonic titers elicited in response to asymptomatic carriage in adults with and without type 2-diabetes. Level of IgG to capsular polysaccharide was measured in a total of 176 samples (124 with type 2 diabetes and 52 without type 2 diabetes) against serotype 1, 19F, 9V, and 18C. From within 176 samples, a nested cohort of 39 samples was selected for measuring the functional capacity of antibodies by measuring opsonic titer to serotypes 19F, 9V, and 18C. Next, we measured levels of IgG to PspA in 90 samples from individuals with and without diabetes (22 non-diabetes and 68 diabetes). Our results demonstrated comparable IgG titers against all serotypes between those with and without type 2-diabetes. Overall, we observed higher opsonic titers in those without diabetes as compared to individuals with diabetes for serotypes 19F and 9V. The opsonic titers for 19F and 9V significantly negatively correlated with HbA1c. For 19F, 41.66% (n = 10) showed opsonic titers ≥ 1:8 in the diabetes group as compared to 66.66% (n = 10) in the non-diabetes group. The percentage was 29.6% (n = 7) vs 66.66% (n = 10) for 9V and 70.83% (n = 17) vs 80% (n = 12) for 18C in diabetes and non-diabetes groups respectively. A comparable anti-PspA IgG (p = 0.409) was observed in those with and without diabetes, indicating that response to protein antigen is likely to remain intact in those with diabetes. In conclusion, we demonstrated comparable IgG titers to both capsular polysaccharide and protein antigens in those with and without diabetes, however, the protective capacity of antibodies differed between the two groups. |
| doi_str_mv | 10.1371/journal.pone.0306921 |
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The study was designed to measure the baseline serotype-specific anti-capsular IgG concentration and opsonic titers elicited in response to asymptomatic carriage in adults with and without type 2-diabetes. Level of IgG to capsular polysaccharide was measured in a total of 176 samples (124 with type 2 diabetes and 52 without type 2 diabetes) against serotype 1, 19F, 9V, and 18C. From within 176 samples, a nested cohort of 39 samples was selected for measuring the functional capacity of antibodies by measuring opsonic titer to serotypes 19F, 9V, and 18C. Next, we measured levels of IgG to PspA in 90 samples from individuals with and without diabetes (22 non-diabetes and 68 diabetes). Our results demonstrated comparable IgG titers against all serotypes between those with and without type 2-diabetes. Overall, we observed higher opsonic titers in those without diabetes as compared to individuals with diabetes for serotypes 19F and 9V. The opsonic titers for 19F and 9V significantly negatively correlated with HbA1c. For 19F, 41.66% (n = 10) showed opsonic titers ≥ 1:8 in the diabetes group as compared to 66.66% (n = 10) in the non-diabetes group. The percentage was 29.6% (n = 7) vs 66.66% (n = 10) for 9V and 70.83% (n = 17) vs 80% (n = 12) for 18C in diabetes and non-diabetes groups respectively. A comparable anti-PspA IgG (p = 0.409) was observed in those with and without diabetes, indicating that response to protein antigen is likely to remain intact in those with diabetes. In conclusion, we demonstrated comparable IgG titers to both capsular polysaccharide and protein antigens in those with and without diabetes, however, the protective capacity of antibodies differed between the two groups.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0306921</identifier><identifier>PMID: 39121085</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Adult ; Adults ; Aged ; Antibiotics ; Antibodies ; Antibodies, Bacterial - blood ; Antibodies, Bacterial - immunology ; Antigens ; Asymptomatic ; Bacteria ; Capsular polysaccharides ; Diabetes ; Diabetes mellitus ; Diabetes mellitus (non-insulin dependent) ; Diabetes Mellitus, Type 2 - blood ; Diabetes Mellitus, Type 2 - immunology ; Diabetes Mellitus, Type 2 - microbiology ; Female ; Glycosylated hemoglobin ; Heat ; Humans ; Immune response ; Immunization ; Immunoglobulin G ; Immunoglobulin G - blood ; Immunoglobulin G - immunology ; Male ; Medical research ; Medicine, Experimental ; Middle Aged ; Pakistan - epidemiology ; Pneumococcal Infections - immunology ; Pneumococcal Infections - microbiology ; Pneumonia ; Polysaccharides ; Proteins ; PspA protein ; Serogroup ; Serotypes ; Streptococcus infections ; Streptococcus pneumoniae ; Streptococcus pneumoniae - immunology ; Vaccines ; Virulence</subject><ispartof>PloS one, 2024-08, Vol.19 (8), p.e0306921</ispartof><rights>Copyright: © 2024 Ahmad et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.</rights><rights>COPYRIGHT 2024 Public Library of Science</rights><rights>2024 Ahmad et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2024 Ahmad et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c540t-aea012eff141a0a36520db2700913077694afdc1cc5c188393858892bc065e893</cites><orcidid>0000-0002-6651-6101 ; 0000-0003-3371-3501</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0306921&type=printable$$EPDF$$P50$$Gplos$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://journals.plos.org/plosone/article?id=10.1371/journal.pone.0306921$$EHTML$$P50$$Gplos$$Hfree_for_read</linktohtml><link.rule.ids>314,776,780,860,2096,2915,23845,27901,27902,79569,79570</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/39121085$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ahmad, Izaz</creatorcontrib><creatorcontrib>Burton, Robert</creatorcontrib><creatorcontrib>Nahm, Moon</creatorcontrib><creatorcontrib>Ejaz, Hafiz Gohar</creatorcontrib><creatorcontrib>Arshad, Rozina</creatorcontrib><creatorcontrib>Younis, Bilal Bin</creatorcontrib><creatorcontrib>Mirza, Shaper</creatorcontrib><title>Naturally acquired antibodies against 4 Streptococcus pneumoniae serotypes in Pakistani adults with type 2 diabetes mellitus</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Immune response elicited during pneumococcal carriage has been shown to protect against subsequent colonization and infection by Streptococcus pneumoniae. 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The opsonic titers for 19F and 9V significantly negatively correlated with HbA1c. For 19F, 41.66% (n = 10) showed opsonic titers ≥ 1:8 in the diabetes group as compared to 66.66% (n = 10) in the non-diabetes group. The percentage was 29.6% (n = 7) vs 66.66% (n = 10) for 9V and 70.83% (n = 17) vs 80% (n = 12) for 18C in diabetes and non-diabetes groups respectively. A comparable anti-PspA IgG (p = 0.409) was observed in those with and without diabetes, indicating that response to protein antigen is likely to remain intact in those with diabetes. In conclusion, we demonstrated comparable IgG titers to both capsular polysaccharide and protein antigens in those with and without diabetes, however, the protective capacity of antibodies differed between the two groups.</description><subject>Adult</subject><subject>Adults</subject><subject>Aged</subject><subject>Antibiotics</subject><subject>Antibodies</subject><subject>Antibodies, Bacterial - blood</subject><subject>Antibodies, Bacterial - immunology</subject><subject>Antigens</subject><subject>Asymptomatic</subject><subject>Bacteria</subject><subject>Capsular polysaccharides</subject><subject>Diabetes</subject><subject>Diabetes mellitus</subject><subject>Diabetes mellitus (non-insulin dependent)</subject><subject>Diabetes Mellitus, Type 2 - blood</subject><subject>Diabetes Mellitus, Type 2 - immunology</subject><subject>Diabetes Mellitus, Type 2 - microbiology</subject><subject>Female</subject><subject>Glycosylated hemoglobin</subject><subject>Heat</subject><subject>Humans</subject><subject>Immune response</subject><subject>Immunization</subject><subject>Immunoglobulin G</subject><subject>Immunoglobulin G - blood</subject><subject>Immunoglobulin G - immunology</subject><subject>Male</subject><subject>Medical research</subject><subject>Medicine, Experimental</subject><subject>Middle Aged</subject><subject>Pakistan - epidemiology</subject><subject>Pneumococcal Infections - immunology</subject><subject>Pneumococcal Infections - microbiology</subject><subject>Pneumonia</subject><subject>Polysaccharides</subject><subject>Proteins</subject><subject>PspA protein</subject><subject>Serogroup</subject><subject>Serotypes</subject><subject>Streptococcus infections</subject><subject>Streptococcus pneumoniae</subject><subject>Streptococcus pneumoniae - immunology</subject><subject>Vaccines</subject><subject>Virulence</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><sourceid>DOA</sourceid><recordid>eNqNkltrFDEYhgdRbK3-A9FAQfRi1xwmc7gsxcNCsWLV2_BNktnNOjOZ5oAu-OPNuNPSlV6YXCQkz_t9ycubZc8JXhJWkrdbG90A3XK0g15ihouakgfZMakZXRQUs4d39kfZE--3GHNWFcXj7IjVhBJc8ePs9ycI0UHX7RDI62icVgiGYBqrjPYI1mAGH1COroLTY7DSShk9GgcdezsY0MhrZ8NuTLAZ0Gf4YXyAwSBQsQse_TRhg6ZrRJEy0OiQwF53nQnRP80etdB5_WxeT7Jv7999Pf-4uLj8sDo_u1hInuOwAA2YUN22JCeAgRWcYtXQEuOaMFyWRZ1DqySRkktSVaxmFa-qmjYSF1xXNTvJXu7rjp31YjbOC5b0BJe8nIjVnlAWtmJ0pge3ExaM-Htg3VqAC0Z2WhQqz1XLeUl1metKNQU0aRSqJE2tc5pqvZ67OXsdtQ-iN16mL8OgbZzbclyVE3r6D3r_42ZqDam_GVobHMipqDircM5KWvMiUct7qDSV7o1MIWlNOj8QvDkQJCboX2EN0Xuxuvry_-zl90P21R12o6ELG2-7GIwd_CGY70HprPdOt7fGEyymjN-4IaaMiznjSfZiNi02vVa3optQsz8gZfay</recordid><startdate>20240809</startdate><enddate>20240809</enddate><creator>Ahmad, Izaz</creator><creator>Burton, Robert</creator><creator>Nahm, Moon</creator><creator>Ejaz, Hafiz Gohar</creator><creator>Arshad, Rozina</creator><creator>Younis, Bilal Bin</creator><creator>Mirza, Shaper</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>COVID</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PHGZM</scope><scope>PHGZT</scope><scope>PIMPY</scope><scope>PJZUB</scope><scope>PKEHL</scope><scope>PPXIY</scope><scope>PQEST</scope><scope>PQGLB</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0002-6651-6101</orcidid><orcidid>https://orcid.org/0000-0003-3371-3501</orcidid></search><sort><creationdate>20240809</creationdate><title>Naturally acquired antibodies against 4 Streptococcus pneumoniae serotypes in Pakistani adults with type 2 diabetes mellitus</title><author>Ahmad, Izaz ; Burton, Robert ; Nahm, Moon ; Ejaz, Hafiz Gohar ; Arshad, Rozina ; Younis, Bilal Bin ; Mirza, Shaper</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c540t-aea012eff141a0a36520db2700913077694afdc1cc5c188393858892bc065e893</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Adult</topic><topic>Adults</topic><topic>Aged</topic><topic>Antibiotics</topic><topic>Antibodies</topic><topic>Antibodies, Bacterial - 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The study was designed to measure the baseline serotype-specific anti-capsular IgG concentration and opsonic titers elicited in response to asymptomatic carriage in adults with and without type 2-diabetes. Level of IgG to capsular polysaccharide was measured in a total of 176 samples (124 with type 2 diabetes and 52 without type 2 diabetes) against serotype 1, 19F, 9V, and 18C. From within 176 samples, a nested cohort of 39 samples was selected for measuring the functional capacity of antibodies by measuring opsonic titer to serotypes 19F, 9V, and 18C. Next, we measured levels of IgG to PspA in 90 samples from individuals with and without diabetes (22 non-diabetes and 68 diabetes). Our results demonstrated comparable IgG titers against all serotypes between those with and without type 2-diabetes. Overall, we observed higher opsonic titers in those without diabetes as compared to individuals with diabetes for serotypes 19F and 9V. The opsonic titers for 19F and 9V significantly negatively correlated with HbA1c. For 19F, 41.66% (n = 10) showed opsonic titers ≥ 1:8 in the diabetes group as compared to 66.66% (n = 10) in the non-diabetes group. The percentage was 29.6% (n = 7) vs 66.66% (n = 10) for 9V and 70.83% (n = 17) vs 80% (n = 12) for 18C in diabetes and non-diabetes groups respectively. A comparable anti-PspA IgG (p = 0.409) was observed in those with and without diabetes, indicating that response to protein antigen is likely to remain intact in those with diabetes. In conclusion, we demonstrated comparable IgG titers to both capsular polysaccharide and protein antigens in those with and without diabetes, however, the protective capacity of antibodies differed between the two groups.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>39121085</pmid><doi>10.1371/journal.pone.0306921</doi><tpages>e0306921</tpages><orcidid>https://orcid.org/0000-0002-6651-6101</orcidid><orcidid>https://orcid.org/0000-0003-3371-3501</orcidid><oa>free_for_read</oa></addata></record> |
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| recordid | cdi_plos_journals_3091107579 |
| source | Public Library of Science (PLoS) Journals Open Access; MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Adult Adults Aged Antibiotics Antibodies Antibodies, Bacterial - blood Antibodies, Bacterial - immunology Antigens Asymptomatic Bacteria Capsular polysaccharides Diabetes Diabetes mellitus Diabetes mellitus (non-insulin dependent) Diabetes Mellitus, Type 2 - blood Diabetes Mellitus, Type 2 - immunology Diabetes Mellitus, Type 2 - microbiology Female Glycosylated hemoglobin Heat Humans Immune response Immunization Immunoglobulin G Immunoglobulin G - blood Immunoglobulin G - immunology Male Medical research Medicine, Experimental Middle Aged Pakistan - epidemiology Pneumococcal Infections - immunology Pneumococcal Infections - microbiology Pneumonia Polysaccharides Proteins PspA protein Serogroup Serotypes Streptococcus infections Streptococcus pneumoniae Streptococcus pneumoniae - immunology Vaccines Virulence |
| title | Naturally acquired antibodies against 4 Streptococcus pneumoniae serotypes in Pakistani adults with type 2 diabetes mellitus |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-21T20%3A58%3A26IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Naturally%20acquired%20antibodies%20against%204%20Streptococcus%20pneumoniae%20serotypes%20in%20Pakistani%20adults%20with%20type%202%20diabetes%20mellitus&rft.jtitle=PloS%20one&rft.au=Ahmad,%20Izaz&rft.date=2024-08-09&rft.volume=19&rft.issue=8&rft.spage=e0306921&rft.pages=e0306921-&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0306921&rft_dat=%3Cgale_plos_%3EA804372956%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=3091107579&rft_id=info:pmid/39121085&rft_galeid=A804372956&rft_doaj_id=oai_doaj_org_article_6d44df5572e74e8db6abbbb6d71b9e42&rfr_iscdi=true |