A novel system to culture human intestinal organoids under physiological oxygen content to study microbial-host interaction

Mechanistic investigation of host-microbe interactions in the human gut are hindered by difficulty of co-culturing microbes with intestinal epithelial cells. On one hand the gut bacteria are a mix of facultative, aerotolerant or obligate anaerobes, while the intestinal epithelium requires oxygen for...

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Veröffentlicht in:	PloS one 2024-07, Vol.19 (7), p.e0300666
Hauptverfasser:	Fofanova, Tatiana Y, Karandikar, Umesh C, Auchtung, Jennifer M, Wilson, Reid L, Valentin, Antonio J, Britton, Robert A, Grande-Allen, K Jane, Estes, Mary K, Hoffman, Kristi, Ramani, Sashirekha, Stewart, Christopher J, Petrosino, Joseph F
Format:	Artikel
Sprache:	eng
Schlagworte:	Anaerobes Anaerobic bacteria Anaerobic conditions Anaerobic microorganisms Bacteria Bacteria, Anaerobic - growth & development Bacteria, Anaerobic - metabolism Bacteroides thetaiotaomicron - metabolism Biology and Life Sciences Cell culture Coculture Techniques - methods Digestive system Epithelial cells Epithelium Gastrointestinal Microbiome Gastrointestinal tract Gene expression Genes Host Microbial Interactions Humans Hypoxia Immunomodulation In vivo methods and tests Intestinal Mucosa - cytology Intestinal Mucosa - metabolism Intestinal Mucosa - microbiology Intestine Intestines - cytology Intestines - microbiology Medicine and Health Sciences Microbiomes Microbiota (Symbiotic organisms) Microorganisms Morphology Organoids Organoids - metabolism Organoids - microbiology Oxygen Oxygen - metabolism Oxygen content Oxygen requirement Physical Sciences Physiological aspects Physiology Research and Analysis Methods Transcriptomics
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