Field study examining the mucosal microbiome in equine glandular gastric disease
Equine glandular gastric disease (EGGD) is a common disease among athletic horses that can negatively impact health and performance. The pathophysiology of this EGGD remains poorly understood. Previous studies using controlled populations of horses identified differences in the gastric glandular muc...
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| Veröffentlicht in: | PloS one 2023-12, Vol.18 (12), p.e0295697-e0295697 |
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| creator | Paul, Linda J Ericsson, Aaron C Andrews, Frank M McAdams, Zachary Keowen, Michael L St Blanc, Michael P Banse, Heidi E |
| description | Equine glandular gastric disease (EGGD) is a common disease among athletic horses that can negatively impact health and performance. The pathophysiology of this EGGD remains poorly understood. Previous studies using controlled populations of horses identified differences in the gastric glandular mucosal microbiome associated with disease. The objective of this study was to compare the gastric microbiome in horses with EGGD and those without across multiple barns and differing management practices. We hypothesized that alterations in the microbiome of the gastric glandular mucosa are associated with EGGD. A secondary objective was to perform a risk factor analysis for EGGD using the diet and management data collected. Microbial populations of biopsies from normal pyloric mucosa of horses without EGGD (control biopsies), normal pyloric mucosa of horses with EGGD (normal biopsies) and areas of glandular mucosal disruption in horses with EGGD (lesion biopsies) were compared. Lesion biopsies had a different microbial community structure than control biopsies. Control biopsies had a higher read count for the phylum Actinomycetota compared to lesion biopsies. Control biopsies also had an enrichment of the genera Staphylococcus and Lawsonella and the species Streptococcus salivarius. Lesion biopsies had an enrichment of the genera Lactobacillus and Actinobacillus and the species Lactobacillus equigenerosi. These results demonstrate differences in the gastric glandular microbiome between sites of disrupted mucosa in horses with EGGD compared to pyloric mucosa of horses without EGGD. Risk factor analysis indicated that exercise duration per week was a risk factor for EGGD. |
| doi_str_mv | 10.1371/journal.pone.0295697 |
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The pathophysiology of this EGGD remains poorly understood. Previous studies using controlled populations of horses identified differences in the gastric glandular mucosal microbiome associated with disease. The objective of this study was to compare the gastric microbiome in horses with EGGD and those without across multiple barns and differing management practices. We hypothesized that alterations in the microbiome of the gastric glandular mucosa are associated with EGGD. A secondary objective was to perform a risk factor analysis for EGGD using the diet and management data collected. Microbial populations of biopsies from normal pyloric mucosa of horses without EGGD (control biopsies), normal pyloric mucosa of horses with EGGD (normal biopsies) and areas of glandular mucosal disruption in horses with EGGD (lesion biopsies) were compared. Lesion biopsies had a different microbial community structure than control biopsies. Control biopsies had a higher read count for the phylum Actinomycetota compared to lesion biopsies. Control biopsies also had an enrichment of the genera Staphylococcus and Lawsonella and the species Streptococcus salivarius. Lesion biopsies had an enrichment of the genera Lactobacillus and Actinobacillus and the species Lactobacillus equigenerosi. These results demonstrate differences in the gastric glandular microbiome between sites of disrupted mucosa in horses with EGGD compared to pyloric mucosa of horses without EGGD. Risk factor analysis indicated that exercise duration per week was a risk factor for EGGD.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0295697</identifier><identifier>PMID: 38060520</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Animals ; Biopsy ; Care and treatment ; Community structure ; Diagnosis ; Diet ; Discriminant analysis ; Disease management ; Endoscopy ; Factor analysis ; Gastric Mucosa - pathology ; Gastrointestinal diseases ; Health aspects ; Horse Diseases - pathology ; Horses ; Lactobacillus ; Lesions ; Mann-Whitney U test ; Medical research ; Medicine, Experimental ; Microbiomes ; Microbiota ; Microbiota (Symbiotic organisms) ; Microorganisms ; Mucosa ; Pathophysiology ; Phenylbutazone ; Population studies ; Populations ; Regression analysis ; Risk analysis ; Risk Factors ; Stomach Diseases - pathology ; Stomach Ulcer - pathology</subject><ispartof>PloS one, 2023-12, Vol.18 (12), p.e0295697-e0295697</ispartof><rights>Copyright: © 2023 Paul et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.</rights><rights>COPYRIGHT 2023 Public Library of Science</rights><rights>2023 Paul et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2023 Paul et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c586t-f9e0a5d160495fa08b4fd33817d4221d0e150d72ba2dbefc06fccce6f2ea84183</cites><orcidid>0000-0002-1298-5615 ; 0000-0003-2883-507X</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0295697&type=printable$$EPDF$$P50$$Gplos$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://journals.plos.org/plosone/article?id=10.1371/journal.pone.0295697$$EHTML$$P50$$Gplos$$Hfree_for_read</linktohtml><link.rule.ids>314,780,784,864,2102,2928,23866,27924,27925,79600,79601</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/38060520$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Suchodolski, Jan S.</contributor><creatorcontrib>Paul, Linda J</creatorcontrib><creatorcontrib>Ericsson, Aaron C</creatorcontrib><creatorcontrib>Andrews, Frank M</creatorcontrib><creatorcontrib>McAdams, Zachary</creatorcontrib><creatorcontrib>Keowen, Michael L</creatorcontrib><creatorcontrib>St Blanc, Michael P</creatorcontrib><creatorcontrib>Banse, Heidi E</creatorcontrib><title>Field study examining the mucosal microbiome in equine glandular gastric disease</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Equine glandular gastric disease (EGGD) is a common disease among athletic horses that can negatively impact health and performance. The pathophysiology of this EGGD remains poorly understood. Previous studies using controlled populations of horses identified differences in the gastric glandular mucosal microbiome associated with disease. The objective of this study was to compare the gastric microbiome in horses with EGGD and those without across multiple barns and differing management practices. We hypothesized that alterations in the microbiome of the gastric glandular mucosa are associated with EGGD. A secondary objective was to perform a risk factor analysis for EGGD using the diet and management data collected. Microbial populations of biopsies from normal pyloric mucosa of horses without EGGD (control biopsies), normal pyloric mucosa of horses with EGGD (normal biopsies) and areas of glandular mucosal disruption in horses with EGGD (lesion biopsies) were compared. Lesion biopsies had a different microbial community structure than control biopsies. 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Risk factor analysis indicated that exercise duration per week was a risk factor for EGGD.</description><subject>Animals</subject><subject>Biopsy</subject><subject>Care and treatment</subject><subject>Community structure</subject><subject>Diagnosis</subject><subject>Diet</subject><subject>Discriminant analysis</subject><subject>Disease management</subject><subject>Endoscopy</subject><subject>Factor analysis</subject><subject>Gastric Mucosa - pathology</subject><subject>Gastrointestinal diseases</subject><subject>Health aspects</subject><subject>Horse Diseases - pathology</subject><subject>Horses</subject><subject>Lactobacillus</subject><subject>Lesions</subject><subject>Mann-Whitney U test</subject><subject>Medical research</subject><subject>Medicine, Experimental</subject><subject>Microbiomes</subject><subject>Microbiota</subject><subject>Microbiota (Symbiotic organisms)</subject><subject>Microorganisms</subject><subject>Mucosa</subject><subject>Pathophysiology</subject><subject>Phenylbutazone</subject><subject>Population studies</subject><subject>Populations</subject><subject>Regression analysis</subject><subject>Risk analysis</subject><subject>Risk Factors</subject><subject>Stomach Diseases - 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The pathophysiology of this EGGD remains poorly understood. Previous studies using controlled populations of horses identified differences in the gastric glandular mucosal microbiome associated with disease. The objective of this study was to compare the gastric microbiome in horses with EGGD and those without across multiple barns and differing management practices. We hypothesized that alterations in the microbiome of the gastric glandular mucosa are associated with EGGD. A secondary objective was to perform a risk factor analysis for EGGD using the diet and management data collected. Microbial populations of biopsies from normal pyloric mucosa of horses without EGGD (control biopsies), normal pyloric mucosa of horses with EGGD (normal biopsies) and areas of glandular mucosal disruption in horses with EGGD (lesion biopsies) were compared. Lesion biopsies had a different microbial community structure than control biopsies. Control biopsies had a higher read count for the phylum Actinomycetota compared to lesion biopsies. Control biopsies also had an enrichment of the genera Staphylococcus and Lawsonella and the species Streptococcus salivarius. Lesion biopsies had an enrichment of the genera Lactobacillus and Actinobacillus and the species Lactobacillus equigenerosi. These results demonstrate differences in the gastric glandular microbiome between sites of disrupted mucosa in horses with EGGD compared to pyloric mucosa of horses without EGGD. Risk factor analysis indicated that exercise duration per week was a risk factor for EGGD.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>38060520</pmid><doi>10.1371/journal.pone.0295697</doi><tpages>e0295697</tpages><orcidid>https://orcid.org/0000-0002-1298-5615</orcidid><orcidid>https://orcid.org/0000-0003-2883-507X</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Animals Biopsy Care and treatment Community structure Diagnosis Diet Discriminant analysis Disease management Endoscopy Factor analysis Gastric Mucosa - pathology Gastrointestinal diseases Health aspects Horse Diseases - pathology Horses Lactobacillus Lesions Mann-Whitney U test Medical research Medicine, Experimental Microbiomes Microbiota Microbiota (Symbiotic organisms) Microorganisms Mucosa Pathophysiology Phenylbutazone Population studies Populations Regression analysis Risk analysis Risk Factors Stomach Diseases - pathology Stomach Ulcer - pathology |
| title | Field study examining the mucosal microbiome in equine glandular gastric disease |
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