Effects of allopurinol and febuxostat on uric acid transport and transporter expression in human umbilical vein endothelial cells
Uric acid induces radical oxygen species formation, endothelial inflammation, and endothelial dysfunction which contributes to the progression of atherosclerosis. Febuxostat inhibits BCRP- and allopurinol stimulates MRP4-mediated uric acid efflux in human embryonic kidney cells. We hypothesized that...
Gespeichert in:
| Veröffentlicht in: | PloS one 2024-06, Vol.19 (6), p.e0305906 |
|---|---|
| Hauptverfasser: | , , , , , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | |
|---|---|
| container_issue | 6 |
| container_start_page | e0305906 |
| container_title | PloS one |
| container_volume | 19 |
| creator | van der Pol, Karel H Koenderink, Jan van den Heuvel, Jeroen J M W van den Broek, Petra Peters, Janny van Bunningen, Imke D W Pertijs, Jeanne Russel, Frans G M Koldenhof, Jim Morshuis, Wim J van Drongelen, Joris Schirris, Tom J J van der Meer, Andries Rongen, Gerard A |
| description | Uric acid induces radical oxygen species formation, endothelial inflammation, and endothelial dysfunction which contributes to the progression of atherosclerosis. Febuxostat inhibits BCRP- and allopurinol stimulates MRP4-mediated uric acid efflux in human embryonic kidney cells. We hypothesized that endothelial cells express uric acid transporters that regulate intracellular uric acid concentration and that modulation of these transporters by febuxostat and allopurinol contributes to their different impact on cardiovascular mortality. The aim of this study was to explore a potential difference between the effect of febuxostat and allopurinol on uric acid uptake by human umbilical vein endothelial cells. Febuxostat increased intracellular uric acid concentrations compared with control. In contrast, allopurinol did not affect intracellular uric acid concentration. In line with this observation, febuxostat increased mRNA expression of GLUT9 and reduced MRP4 expression, while allopurinol did not affect mRNA expression of these uric acid transporters. These findings provide a possible pathophysiological pathway which could explain the higher cardiovascular mortality for febuxostat compared to allopurinol but should be explored further. |
| doi_str_mv | 10.1371/journal.pone.0305906 |
| format | Article |
| fullrecord | <record><control><sourceid>gale_plos_</sourceid><recordid>TN_cdi_plos_journals_3071023436</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A798527605</galeid><doaj_id>oai_doaj_org_article_fd27e0ee1021449984bd8991649ed5a0</doaj_id><sourcerecordid>A798527605</sourcerecordid><originalsourceid>FETCH-LOGICAL-c642t-5bfe546f1857ccf3bc8c007ebef7db58b0e816ca6f3c20cc7fc555b6879684b53</originalsourceid><addsrcrecordid>eNqNk02P0zAQhiMEYpfCP0AQCQnBocWOYyc5odVqgUorrcTX1XKccevKsYvtrMqRf467zVYN2gPKwfH4eWfs154se4nRApMKf9i4wVthFltnYYEIog1ij7Jz3JBizgpEHp_8n2XPQtggREnN2NPsjNQNogXC59mfK6VAxpA7lQtj3Hbw2jqTC9vlCtph50IUMXc2TwsyF1J3efTChq3z8Y46zsDnsNt6CEEnXNt8PfQi6fpWGy2FyW8hBcF2Lq7B6BSQYEx4nj1RwgR4MY6z7Menq--XX-bXN5-XlxfXc8nKIs5pq4CWTOGaVlIq0spaIlRBC6rqWlq3CGrMpGCKyAJJWSlJKW1ZXTWsLltKZtnrQ96tcYGP7gVOUIVRQUrCErE8EJ0TG771uhf-N3dC87uA8ysufNTSAFddUQECSFJclk2TKnR102BWNtBRgVKuj2O1oe2hk2CTT2aSdLpi9Zqv3C3HGDdFmXY0y96NGbz7NUCIvNdh75iw4IZx4zUrCU7om3_Qh483UiuRTqCtcqmw3CflF1VT06JiaG_T4gEqfR30Wqa3pnSKTwTvJ4LERNjFlRhC4MtvX_-fvfk5Zd-esGsQJq6DM0NMrytMwfIASu9C8KCOLmPE961y7wbftwofWyXJXp3e0FF03xvkL4Z0EPY</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>3071023436</pqid></control><display><type>article</type><title>Effects of allopurinol and febuxostat on uric acid transport and transporter expression in human umbilical vein endothelial cells</title><source>Public Library of Science (PLoS) Journals Open Access</source><source>MEDLINE</source><source>DOAJ Directory of Open Access Journals</source><source>EZB-FREE-00999 freely available EZB journals</source><source>PubMed Central</source><source>Free Full-Text Journals in Chemistry</source><creator>van der Pol, Karel H ; Koenderink, Jan ; van den Heuvel, Jeroen J M W ; van den Broek, Petra ; Peters, Janny ; van Bunningen, Imke D W ; Pertijs, Jeanne ; Russel, Frans G M ; Koldenhof, Jim ; Morshuis, Wim J ; van Drongelen, Joris ; Schirris, Tom J J ; van der Meer, Andries ; Rongen, Gerard A</creator><creatorcontrib>van der Pol, Karel H ; Koenderink, Jan ; van den Heuvel, Jeroen J M W ; van den Broek, Petra ; Peters, Janny ; van Bunningen, Imke D W ; Pertijs, Jeanne ; Russel, Frans G M ; Koldenhof, Jim ; Morshuis, Wim J ; van Drongelen, Joris ; Schirris, Tom J J ; van der Meer, Andries ; Rongen, Gerard A</creatorcontrib><description>Uric acid induces radical oxygen species formation, endothelial inflammation, and endothelial dysfunction which contributes to the progression of atherosclerosis. Febuxostat inhibits BCRP- and allopurinol stimulates MRP4-mediated uric acid efflux in human embryonic kidney cells. We hypothesized that endothelial cells express uric acid transporters that regulate intracellular uric acid concentration and that modulation of these transporters by febuxostat and allopurinol contributes to their different impact on cardiovascular mortality. The aim of this study was to explore a potential difference between the effect of febuxostat and allopurinol on uric acid uptake by human umbilical vein endothelial cells. Febuxostat increased intracellular uric acid concentrations compared with control. In contrast, allopurinol did not affect intracellular uric acid concentration. In line with this observation, febuxostat increased mRNA expression of GLUT9 and reduced MRP4 expression, while allopurinol did not affect mRNA expression of these uric acid transporters. These findings provide a possible pathophysiological pathway which could explain the higher cardiovascular mortality for febuxostat compared to allopurinol but should be explored further.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0305906</identifier><identifier>PMID: 38905201</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Allopurinol ; Allopurinol - pharmacology ; Analysis ; Arteriosclerosis ; Atherosclerosis ; Biological Transport - drug effects ; Biology and Life Sciences ; Cardiovascular diseases ; Clinical trials ; Development and progression ; Disease ; Efflux ; Endothelial cells ; Endothelium ; Epidermal growth factor ; Febuxostat - pharmacology ; Gene expression ; Gene Expression Regulation - drug effects ; Glucose ; Glucose Transport Proteins, Facilitative - genetics ; Glucose Transport Proteins, Facilitative - metabolism ; Health aspects ; Hospitals ; Human Umbilical Vein Endothelial Cells - drug effects ; Human Umbilical Vein Endothelial Cells - metabolism ; Humans ; Inflammation ; Intracellular ; Kidneys ; Medicine and Health Sciences ; Mortality ; Multidrug Resistance-Associated Proteins - genetics ; Multidrug Resistance-Associated Proteins - metabolism ; Penicillin ; Physical Sciences ; Proteins ; RNA ; RNA, Messenger - genetics ; RNA, Messenger - metabolism ; Thoracic surgery ; Umbilical cord ; Umbilical vein ; Uric acid ; Uric Acid - metabolism ; Veins & arteries</subject><ispartof>PloS one, 2024-06, Vol.19 (6), p.e0305906</ispartof><rights>Copyright: © 2024 van der Pol et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.</rights><rights>COPYRIGHT 2024 Public Library of Science</rights><rights>2024 van der Pol et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2024 van der Pol et al 2024 van der Pol et al</rights><rights>2024 van der Pol et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c642t-5bfe546f1857ccf3bc8c007ebef7db58b0e816ca6f3c20cc7fc555b6879684b53</cites><orcidid>0000-0002-3064-3192 ; 0000-0002-7959-2314 ; 0000-0001-5345-9788</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC11192402/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC11192402/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,2096,2915,23845,27901,27902,53766,53768,79342,79343</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/38905201$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>van der Pol, Karel H</creatorcontrib><creatorcontrib>Koenderink, Jan</creatorcontrib><creatorcontrib>van den Heuvel, Jeroen J M W</creatorcontrib><creatorcontrib>van den Broek, Petra</creatorcontrib><creatorcontrib>Peters, Janny</creatorcontrib><creatorcontrib>van Bunningen, Imke D W</creatorcontrib><creatorcontrib>Pertijs, Jeanne</creatorcontrib><creatorcontrib>Russel, Frans G M</creatorcontrib><creatorcontrib>Koldenhof, Jim</creatorcontrib><creatorcontrib>Morshuis, Wim J</creatorcontrib><creatorcontrib>van Drongelen, Joris</creatorcontrib><creatorcontrib>Schirris, Tom J J</creatorcontrib><creatorcontrib>van der Meer, Andries</creatorcontrib><creatorcontrib>Rongen, Gerard A</creatorcontrib><title>Effects of allopurinol and febuxostat on uric acid transport and transporter expression in human umbilical vein endothelial cells</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Uric acid induces radical oxygen species formation, endothelial inflammation, and endothelial dysfunction which contributes to the progression of atherosclerosis. Febuxostat inhibits BCRP- and allopurinol stimulates MRP4-mediated uric acid efflux in human embryonic kidney cells. We hypothesized that endothelial cells express uric acid transporters that regulate intracellular uric acid concentration and that modulation of these transporters by febuxostat and allopurinol contributes to their different impact on cardiovascular mortality. The aim of this study was to explore a potential difference between the effect of febuxostat and allopurinol on uric acid uptake by human umbilical vein endothelial cells. Febuxostat increased intracellular uric acid concentrations compared with control. In contrast, allopurinol did not affect intracellular uric acid concentration. In line with this observation, febuxostat increased mRNA expression of GLUT9 and reduced MRP4 expression, while allopurinol did not affect mRNA expression of these uric acid transporters. These findings provide a possible pathophysiological pathway which could explain the higher cardiovascular mortality for febuxostat compared to allopurinol but should be explored further.</description><subject>Allopurinol</subject><subject>Allopurinol - pharmacology</subject><subject>Analysis</subject><subject>Arteriosclerosis</subject><subject>Atherosclerosis</subject><subject>Biological Transport - drug effects</subject><subject>Biology and Life Sciences</subject><subject>Cardiovascular diseases</subject><subject>Clinical trials</subject><subject>Development and progression</subject><subject>Disease</subject><subject>Efflux</subject><subject>Endothelial cells</subject><subject>Endothelium</subject><subject>Epidermal growth factor</subject><subject>Febuxostat - pharmacology</subject><subject>Gene expression</subject><subject>Gene Expression Regulation - drug effects</subject><subject>Glucose</subject><subject>Glucose Transport Proteins, Facilitative - genetics</subject><subject>Glucose Transport Proteins, Facilitative - metabolism</subject><subject>Health aspects</subject><subject>Hospitals</subject><subject>Human Umbilical Vein Endothelial Cells - drug effects</subject><subject>Human Umbilical Vein Endothelial Cells - metabolism</subject><subject>Humans</subject><subject>Inflammation</subject><subject>Intracellular</subject><subject>Kidneys</subject><subject>Medicine and Health Sciences</subject><subject>Mortality</subject><subject>Multidrug Resistance-Associated Proteins - genetics</subject><subject>Multidrug Resistance-Associated Proteins - metabolism</subject><subject>Penicillin</subject><subject>Physical Sciences</subject><subject>Proteins</subject><subject>RNA</subject><subject>RNA, Messenger - genetics</subject><subject>RNA, Messenger - metabolism</subject><subject>Thoracic surgery</subject><subject>Umbilical cord</subject><subject>Umbilical vein</subject><subject>Uric acid</subject><subject>Uric Acid - metabolism</subject><subject>Veins & arteries</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><sourceid>DOA</sourceid><recordid>eNqNk02P0zAQhiMEYpfCP0AQCQnBocWOYyc5odVqgUorrcTX1XKccevKsYvtrMqRf467zVYN2gPKwfH4eWfs154se4nRApMKf9i4wVthFltnYYEIog1ij7Jz3JBizgpEHp_8n2XPQtggREnN2NPsjNQNogXC59mfK6VAxpA7lQtj3Hbw2jqTC9vlCtph50IUMXc2TwsyF1J3efTChq3z8Y46zsDnsNt6CEEnXNt8PfQi6fpWGy2FyW8hBcF2Lq7B6BSQYEx4nj1RwgR4MY6z7Menq--XX-bXN5-XlxfXc8nKIs5pq4CWTOGaVlIq0spaIlRBC6rqWlq3CGrMpGCKyAJJWSlJKW1ZXTWsLltKZtnrQ96tcYGP7gVOUIVRQUrCErE8EJ0TG771uhf-N3dC87uA8ysufNTSAFddUQECSFJclk2TKnR102BWNtBRgVKuj2O1oe2hk2CTT2aSdLpi9Zqv3C3HGDdFmXY0y96NGbz7NUCIvNdh75iw4IZx4zUrCU7om3_Qh483UiuRTqCtcqmw3CflF1VT06JiaG_T4gEqfR30Wqa3pnSKTwTvJ4LERNjFlRhC4MtvX_-fvfk5Zd-esGsQJq6DM0NMrytMwfIASu9C8KCOLmPE961y7wbftwofWyXJXp3e0FF03xvkL4Z0EPY</recordid><startdate>20240621</startdate><enddate>20240621</enddate><creator>van der Pol, Karel H</creator><creator>Koenderink, Jan</creator><creator>van den Heuvel, Jeroen J M W</creator><creator>van den Broek, Petra</creator><creator>Peters, Janny</creator><creator>van Bunningen, Imke D W</creator><creator>Pertijs, Jeanne</creator><creator>Russel, Frans G M</creator><creator>Koldenhof, Jim</creator><creator>Morshuis, Wim J</creator><creator>van Drongelen, Joris</creator><creator>Schirris, Tom J J</creator><creator>van der Meer, Andries</creator><creator>Rongen, Gerard A</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0002-3064-3192</orcidid><orcidid>https://orcid.org/0000-0002-7959-2314</orcidid><orcidid>https://orcid.org/0000-0001-5345-9788</orcidid></search><sort><creationdate>20240621</creationdate><title>Effects of allopurinol and febuxostat on uric acid transport and transporter expression in human umbilical vein endothelial cells</title><author>van der Pol, Karel H ; Koenderink, Jan ; van den Heuvel, Jeroen J M W ; van den Broek, Petra ; Peters, Janny ; van Bunningen, Imke D W ; Pertijs, Jeanne ; Russel, Frans G M ; Koldenhof, Jim ; Morshuis, Wim J ; van Drongelen, Joris ; Schirris, Tom J J ; van der Meer, Andries ; Rongen, Gerard A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c642t-5bfe546f1857ccf3bc8c007ebef7db58b0e816ca6f3c20cc7fc555b6879684b53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Allopurinol</topic><topic>Allopurinol - pharmacology</topic><topic>Analysis</topic><topic>Arteriosclerosis</topic><topic>Atherosclerosis</topic><topic>Biological Transport - drug effects</topic><topic>Biology and Life Sciences</topic><topic>Cardiovascular diseases</topic><topic>Clinical trials</topic><topic>Development and progression</topic><topic>Disease</topic><topic>Efflux</topic><topic>Endothelial cells</topic><topic>Endothelium</topic><topic>Epidermal growth factor</topic><topic>Febuxostat - pharmacology</topic><topic>Gene expression</topic><topic>Gene Expression Regulation - drug effects</topic><topic>Glucose</topic><topic>Glucose Transport Proteins, Facilitative - genetics</topic><topic>Glucose Transport Proteins, Facilitative - metabolism</topic><topic>Health aspects</topic><topic>Hospitals</topic><topic>Human Umbilical Vein Endothelial Cells - drug effects</topic><topic>Human Umbilical Vein Endothelial Cells - metabolism</topic><topic>Humans</topic><topic>Inflammation</topic><topic>Intracellular</topic><topic>Kidneys</topic><topic>Medicine and Health Sciences</topic><topic>Mortality</topic><topic>Multidrug Resistance-Associated Proteins - genetics</topic><topic>Multidrug Resistance-Associated Proteins - metabolism</topic><topic>Penicillin</topic><topic>Physical Sciences</topic><topic>Proteins</topic><topic>RNA</topic><topic>RNA, Messenger - genetics</topic><topic>RNA, Messenger - metabolism</topic><topic>Thoracic surgery</topic><topic>Umbilical cord</topic><topic>Umbilical vein</topic><topic>Uric acid</topic><topic>Uric Acid - metabolism</topic><topic>Veins & arteries</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>van der Pol, Karel H</creatorcontrib><creatorcontrib>Koenderink, Jan</creatorcontrib><creatorcontrib>van den Heuvel, Jeroen J M W</creatorcontrib><creatorcontrib>van den Broek, Petra</creatorcontrib><creatorcontrib>Peters, Janny</creatorcontrib><creatorcontrib>van Bunningen, Imke D W</creatorcontrib><creatorcontrib>Pertijs, Jeanne</creatorcontrib><creatorcontrib>Russel, Frans G M</creatorcontrib><creatorcontrib>Koldenhof, Jim</creatorcontrib><creatorcontrib>Morshuis, Wim J</creatorcontrib><creatorcontrib>van Drongelen, Joris</creatorcontrib><creatorcontrib>Schirris, Tom J J</creatorcontrib><creatorcontrib>van der Meer, Andries</creatorcontrib><creatorcontrib>Rongen, Gerard A</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: Opposing Viewpoints</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Nursing & Allied Health Database</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Meteorological & Geoastrophysical Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Meteorological & Geoastrophysical Abstracts - Academic</collection><collection>ProQuest Engineering Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Engineering Database</collection><collection>Nursing & Allied Health Premium</collection><collection>Advanced Technologies & Aerospace Database</collection><collection>ProQuest Advanced Technologies & Aerospace Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>Materials Science Collection</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Engineering Collection</collection><collection>Environmental Science Collection</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>van der Pol, Karel H</au><au>Koenderink, Jan</au><au>van den Heuvel, Jeroen J M W</au><au>van den Broek, Petra</au><au>Peters, Janny</au><au>van Bunningen, Imke D W</au><au>Pertijs, Jeanne</au><au>Russel, Frans G M</au><au>Koldenhof, Jim</au><au>Morshuis, Wim J</au><au>van Drongelen, Joris</au><au>Schirris, Tom J J</au><au>van der Meer, Andries</au><au>Rongen, Gerard A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effects of allopurinol and febuxostat on uric acid transport and transporter expression in human umbilical vein endothelial cells</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2024-06-21</date><risdate>2024</risdate><volume>19</volume><issue>6</issue><spage>e0305906</spage><pages>e0305906-</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Uric acid induces radical oxygen species formation, endothelial inflammation, and endothelial dysfunction which contributes to the progression of atherosclerosis. Febuxostat inhibits BCRP- and allopurinol stimulates MRP4-mediated uric acid efflux in human embryonic kidney cells. We hypothesized that endothelial cells express uric acid transporters that regulate intracellular uric acid concentration and that modulation of these transporters by febuxostat and allopurinol contributes to their different impact on cardiovascular mortality. The aim of this study was to explore a potential difference between the effect of febuxostat and allopurinol on uric acid uptake by human umbilical vein endothelial cells. Febuxostat increased intracellular uric acid concentrations compared with control. In contrast, allopurinol did not affect intracellular uric acid concentration. In line with this observation, febuxostat increased mRNA expression of GLUT9 and reduced MRP4 expression, while allopurinol did not affect mRNA expression of these uric acid transporters. These findings provide a possible pathophysiological pathway which could explain the higher cardiovascular mortality for febuxostat compared to allopurinol but should be explored further.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>38905201</pmid><doi>10.1371/journal.pone.0305906</doi><tpages>e0305906</tpages><orcidid>https://orcid.org/0000-0002-3064-3192</orcidid><orcidid>https://orcid.org/0000-0002-7959-2314</orcidid><orcidid>https://orcid.org/0000-0001-5345-9788</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2024-06, Vol.19 (6), p.e0305906 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_3071023436 |
| source | Public Library of Science (PLoS) Journals Open Access; MEDLINE; DOAJ Directory of Open Access Journals; EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Allopurinol Allopurinol - pharmacology Analysis Arteriosclerosis Atherosclerosis Biological Transport - drug effects Biology and Life Sciences Cardiovascular diseases Clinical trials Development and progression Disease Efflux Endothelial cells Endothelium Epidermal growth factor Febuxostat - pharmacology Gene expression Gene Expression Regulation - drug effects Glucose Glucose Transport Proteins, Facilitative - genetics Glucose Transport Proteins, Facilitative - metabolism Health aspects Hospitals Human Umbilical Vein Endothelial Cells - drug effects Human Umbilical Vein Endothelial Cells - metabolism Humans Inflammation Intracellular Kidneys Medicine and Health Sciences Mortality Multidrug Resistance-Associated Proteins - genetics Multidrug Resistance-Associated Proteins - metabolism Penicillin Physical Sciences Proteins RNA RNA, Messenger - genetics RNA, Messenger - metabolism Thoracic surgery Umbilical cord Umbilical vein Uric acid Uric Acid - metabolism Veins & arteries |
| title | Effects of allopurinol and febuxostat on uric acid transport and transporter expression in human umbilical vein endothelial cells |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-10T13%3A38%3A26IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Effects%20of%20allopurinol%20and%20febuxostat%20on%20uric%20acid%20transport%20and%20transporter%20expression%20in%20human%20umbilical%20vein%20endothelial%20cells&rft.jtitle=PloS%20one&rft.au=van%20der%20Pol,%20Karel%20H&rft.date=2024-06-21&rft.volume=19&rft.issue=6&rft.spage=e0305906&rft.pages=e0305906-&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0305906&rft_dat=%3Cgale_plos_%3EA798527605%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=3071023436&rft_id=info:pmid/38905201&rft_galeid=A798527605&rft_doaj_id=oai_doaj_org_article_fd27e0ee1021449984bd8991649ed5a0&rfr_iscdi=true |