Benzyl isothiocyanate as an alternative to antibiotics? a comparative in vivo study using Pseudomonas aeruginosa infection as a model
Due to over-prescription of antibiotics, antimicrobial resistance has emerged to be a critical concern globally. Many countries have tightened the control of antibiotic usage, which, in turn, promotes the search for alternatives to antibiotics. Quite a few phytochemicals have been investigated. Benz...
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| Veröffentlicht in: | PloS one 2024-05, Vol.19 (5), p.e0303490-e0303490 |
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| creator | Yang, Jian Hediyal, Tousif Ahmed Chidambaram, Saravana Babu Kaul-Ghanekar, Ruchika Sakharkar, Meena Kishore |
| description | Due to over-prescription of antibiotics, antimicrobial resistance has emerged to be a critical concern globally. Many countries have tightened the control of antibiotic usage, which, in turn, promotes the search for alternatives to antibiotics. Quite a few phytochemicals have been investigated. Benzyl isothiocyanate (BITC) is an important secondary metabolite in cruciferous species and exhibited potent antimicrobial activity under in vitro conditions. In this research, we undertook a comparative mouse model study of BITC with gentamycin sulfate (positive antibiotic control) and ceftiofur hydrochloride (negative antibiotic control) against Pseudomonas aeruginosa infection. Our results showed that BITC exhibited comparable or better antimicrobial activity and lower infiltration of mouse immune cells upon comparing to gentamycin sulfate. Furthermore, BITC did not impose any toxicity to the air pouch skin tissues. In summary, our current study suggests that BITC could be an alternative to antibiotics and deserves further in vivo and clinical trial studies. |
| doi_str_mv | 10.1371/journal.pone.0303490 |
| format | Article |
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Many countries have tightened the control of antibiotic usage, which, in turn, promotes the search for alternatives to antibiotics. Quite a few phytochemicals have been investigated. Benzyl isothiocyanate (BITC) is an important secondary metabolite in cruciferous species and exhibited potent antimicrobial activity under in vitro conditions. In this research, we undertook a comparative mouse model study of BITC with gentamycin sulfate (positive antibiotic control) and ceftiofur hydrochloride (negative antibiotic control) against Pseudomonas aeruginosa infection. Our results showed that BITC exhibited comparable or better antimicrobial activity and lower infiltration of mouse immune cells upon comparing to gentamycin sulfate. Furthermore, BITC did not impose any toxicity to the air pouch skin tissues. In summary, our current study suggests that BITC could be an alternative to antibiotics and deserves further in vivo and clinical trial studies.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0303490</identifier><identifier>PMID: 38753636</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Analysis ; Animals ; Anti-Bacterial Agents - pharmacology ; Anti-Bacterial Agents - therapeutic use ; Antibiotics ; Antimicrobial activity ; Antimicrobial agents ; Antimicrobial resistance ; Bacterial infections ; Biocompatibility ; Biology and Life Sciences ; Disease Models, Animal ; Drug resistance in microorganisms ; Ethylenediaminetetraacetic acid ; Female ; Gentamicin ; Health aspects ; Immune system ; In vivo methods and tests ; Infection ; Infections ; Inflammation ; Isothiocyanate ; Isothiocyanates - pharmacology ; Medical research ; Medicine and Health Sciences ; Medicine, Experimental ; Metabolites ; Methicillin ; Mice ; Microbial Sensitivity Tests ; Physical Sciences ; Phytochemicals ; Pseudomonas aeruginosa ; Pseudomonas aeruginosa - drug effects ; Pseudomonas Infections - drug therapy ; Pseudomonas Infections - microbiology ; Research and Analysis Methods ; Skin ; Sulfates ; Toxicity</subject><ispartof>PloS one, 2024-05, Vol.19 (5), p.e0303490-e0303490</ispartof><rights>Copyright: © 2024 Yang et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.</rights><rights>COPYRIGHT 2024 Public Library of Science</rights><rights>2024 Yang et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2024 Yang et al 2024 Yang et al</rights><rights>2024 Yang et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. 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Many countries have tightened the control of antibiotic usage, which, in turn, promotes the search for alternatives to antibiotics. Quite a few phytochemicals have been investigated. Benzyl isothiocyanate (BITC) is an important secondary metabolite in cruciferous species and exhibited potent antimicrobial activity under in vitro conditions. In this research, we undertook a comparative mouse model study of BITC with gentamycin sulfate (positive antibiotic control) and ceftiofur hydrochloride (negative antibiotic control) against Pseudomonas aeruginosa infection. Our results showed that BITC exhibited comparable or better antimicrobial activity and lower infiltration of mouse immune cells upon comparing to gentamycin sulfate. Furthermore, BITC did not impose any toxicity to the air pouch skin tissues. 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yang, Jian</au><au>Hediyal, Tousif Ahmed</au><au>Chidambaram, Saravana Babu</au><au>Kaul-Ghanekar, Ruchika</au><au>Sakharkar, Meena Kishore</au><au>Bakare, Adekunle Akeem</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Benzyl isothiocyanate as an alternative to antibiotics? a comparative in vivo study using Pseudomonas aeruginosa infection as a model</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2024-05-16</date><risdate>2024</risdate><volume>19</volume><issue>5</issue><spage>e0303490</spage><epage>e0303490</epage><pages>e0303490-e0303490</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Due to over-prescription of antibiotics, antimicrobial resistance has emerged to be a critical concern globally. Many countries have tightened the control of antibiotic usage, which, in turn, promotes the search for alternatives to antibiotics. Quite a few phytochemicals have been investigated. Benzyl isothiocyanate (BITC) is an important secondary metabolite in cruciferous species and exhibited potent antimicrobial activity under in vitro conditions. In this research, we undertook a comparative mouse model study of BITC with gentamycin sulfate (positive antibiotic control) and ceftiofur hydrochloride (negative antibiotic control) against Pseudomonas aeruginosa infection. Our results showed that BITC exhibited comparable or better antimicrobial activity and lower infiltration of mouse immune cells upon comparing to gentamycin sulfate. Furthermore, BITC did not impose any toxicity to the air pouch skin tissues. In summary, our current study suggests that BITC could be an alternative to antibiotics and deserves further in vivo and clinical trial studies.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>38753636</pmid><doi>10.1371/journal.pone.0303490</doi><tpages>e0303490</tpages><orcidid>https://orcid.org/0000-0002-4935-7380</orcidid><orcidid>https://orcid.org/0000-0002-9289-3314</orcidid><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; DOAJ Directory of Open Access Journals; Public Library of Science (PLoS) Journals Open Access; EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Analysis Animals Anti-Bacterial Agents - pharmacology Anti-Bacterial Agents - therapeutic use Antibiotics Antimicrobial activity Antimicrobial agents Antimicrobial resistance Bacterial infections Biocompatibility Biology and Life Sciences Disease Models, Animal Drug resistance in microorganisms Ethylenediaminetetraacetic acid Female Gentamicin Health aspects Immune system In vivo methods and tests Infection Infections Inflammation Isothiocyanate Isothiocyanates - pharmacology Medical research Medicine and Health Sciences Medicine, Experimental Metabolites Methicillin Mice Microbial Sensitivity Tests Physical Sciences Phytochemicals Pseudomonas aeruginosa Pseudomonas aeruginosa - drug effects Pseudomonas Infections - drug therapy Pseudomonas Infections - microbiology Research and Analysis Methods Skin Sulfates Toxicity |
| title | Benzyl isothiocyanate as an alternative to antibiotics? a comparative in vivo study using Pseudomonas aeruginosa infection as a model |
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