Age and sex-related variations in murine laryngeal microbiota
The larynx undergoes significant age and sex-related changes in structure and function across the lifespan. Emerging evidence suggests that laryngeal microbiota influences immunological processes. Thus, there is a critical need to delineate microbial mechanisms that may underlie laryngeal physiologi...
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| creator | An, Ran Venkatraman, Anumitha Binns, John Saric, Callie Rey, Federico E Thibeault, Susan L |
| description | The larynx undergoes significant age and sex-related changes in structure and function across the lifespan. Emerging evidence suggests that laryngeal microbiota influences immunological processes. Thus, there is a critical need to delineate microbial mechanisms that may underlie laryngeal physiological and immunological changes. As a first step, the present study explored potential age and sex-related changes in the laryngeal microbiota across the lifespan in a murine model. We compared laryngeal microbial profiles of mice across the lifespan (adolescents, young adults, older adults and elderly) to determine age and sex-related microbial variation on 16s rRNA gene sequencing. Measures of alpha diversity and beta diversity were obtained, along with differentially abundant taxa across age groups and biological sexes. There was relative stability of the laryngeal microbiota within each age group and no significant bacterial compositional shift in the laryngeal microbiome across the lifespan. There was an abundance of short-chain fatty acid producing bacteria in the adolescent group, unique to the laryngeal microbiota; taxonomic changes in the elderly resembled that of the aged gut microbiome. There were no significant changes in the laryngeal microbiota relating to biological sex. This is the first study to report age and sex-related variation in laryngeal microbiota. This data lays the groundwork for defining how age-related microbial mechanisms may govern laryngeal health and disease. Bacterial compositional changes, as a result of environmental or systemic stimuli, may not only be indicative of laryngeal-specific metabolic and immunoregulatory processes, but may precede structural and functional age-related changes in laryngeal physiology. |
| doi_str_mv | 10.1371/journal.pone.0300672 |
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Emerging evidence suggests that laryngeal microbiota influences immunological processes. Thus, there is a critical need to delineate microbial mechanisms that may underlie laryngeal physiological and immunological changes. As a first step, the present study explored potential age and sex-related changes in the laryngeal microbiota across the lifespan in a murine model. We compared laryngeal microbial profiles of mice across the lifespan (adolescents, young adults, older adults and elderly) to determine age and sex-related microbial variation on 16s rRNA gene sequencing. Measures of alpha diversity and beta diversity were obtained, along with differentially abundant taxa across age groups and biological sexes. There was relative stability of the laryngeal microbiota within each age group and no significant bacterial compositional shift in the laryngeal microbiome across the lifespan. There was an abundance of short-chain fatty acid producing bacteria in the adolescent group, unique to the laryngeal microbiota; taxonomic changes in the elderly resembled that of the aged gut microbiome. There were no significant changes in the laryngeal microbiota relating to biological sex. This is the first study to report age and sex-related variation in laryngeal microbiota. This data lays the groundwork for defining how age-related microbial mechanisms may govern laryngeal health and disease. Bacterial compositional changes, as a result of environmental or systemic stimuli, may not only be indicative of laryngeal-specific metabolic and immunoregulatory processes, but may precede structural and functional age-related changes in laryngeal physiology.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0300672</identifier><identifier>PMID: 38743725</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Adolescents ; Adults ; Age ; Age Factors ; Age groups ; Aging - physiology ; Animal models ; Animals ; Bacteria ; Bacteria - classification ; Bacteria - genetics ; Biological diversity ; Biology and Life Sciences ; Comparative analysis ; Environmental effects ; Ethylenediaminetetraacetic acid ; Fatty acids ; Female ; Fourier transforms ; Gender differences ; Gene amplification ; Gene sequencing ; Genetic testing ; Immunology ; Immunoregulation ; Intestinal microflora ; Larynx ; Larynx - microbiology ; Life span ; Male ; Medicine and Health Sciences ; Mice ; Mice, Inbred C57BL ; Microbiomes ; Microbiota ; Microbiota (Symbiotic organisms) ; Microorganisms ; Older people ; Pathogenesis ; Pathology ; People and Places ; Physiological aspects ; Physiology ; RNA ; RNA, Ribosomal, 16S - genetics ; rRNA 16S ; Sex ; Sex Factors ; Sexes ; Structure-function relationships ; Variance analysis ; Variation ; Young adults</subject><ispartof>PloS one, 2024-05, Vol.19 (5), p.e0300672-e0300672</ispartof><rights>Copyright: © 2024 An et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.</rights><rights>COPYRIGHT 2024 Public Library of Science</rights><rights>2024 An et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2024 An et al 2024 An et al</rights><rights>2024 An et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. 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Emerging evidence suggests that laryngeal microbiota influences immunological processes. Thus, there is a critical need to delineate microbial mechanisms that may underlie laryngeal physiological and immunological changes. As a first step, the present study explored potential age and sex-related changes in the laryngeal microbiota across the lifespan in a murine model. We compared laryngeal microbial profiles of mice across the lifespan (adolescents, young adults, older adults and elderly) to determine age and sex-related microbial variation on 16s rRNA gene sequencing. Measures of alpha diversity and beta diversity were obtained, along with differentially abundant taxa across age groups and biological sexes. There was relative stability of the laryngeal microbiota within each age group and no significant bacterial compositional shift in the laryngeal microbiome across the lifespan. There was an abundance of short-chain fatty acid producing bacteria in the adolescent group, unique to the laryngeal microbiota; taxonomic changes in the elderly resembled that of the aged gut microbiome. There were no significant changes in the laryngeal microbiota relating to biological sex. This is the first study to report age and sex-related variation in laryngeal microbiota. This data lays the groundwork for defining how age-related microbial mechanisms may govern laryngeal health and disease. 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| issn | 1932-6203 1932-6203 |
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| subjects | Adolescents Adults Age Age Factors Age groups Aging - physiology Animal models Animals Bacteria Bacteria - classification Bacteria - genetics Biological diversity Biology and Life Sciences Comparative analysis Environmental effects Ethylenediaminetetraacetic acid Fatty acids Female Fourier transforms Gender differences Gene amplification Gene sequencing Genetic testing Immunology Immunoregulation Intestinal microflora Larynx Larynx - microbiology Life span Male Medicine and Health Sciences Mice Mice, Inbred C57BL Microbiomes Microbiota Microbiota (Symbiotic organisms) Microorganisms Older people Pathogenesis Pathology People and Places Physiological aspects Physiology RNA RNA, Ribosomal, 16S - genetics rRNA 16S Sex Sex Factors Sexes Structure-function relationships Variance analysis Variation Young adults |
| title | Age and sex-related variations in murine laryngeal microbiota |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-21T03%3A09%3A31IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Age%20and%20sex-related%20variations%20in%20murine%20laryngeal%20microbiota&rft.jtitle=PloS%20one&rft.au=An,%20Ran&rft.date=2024-05-14&rft.volume=19&rft.issue=5&rft.spage=e0300672&rft.epage=e0300672&rft.pages=e0300672-e0300672&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0300672&rft_dat=%3Cgale_plos_%3EA793817797%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=3069288163&rft_id=info:pmid/38743725&rft_galeid=A793817797&rft_doaj_id=oai_doaj_org_article_8f7a7350b7544c3695f0327c6372c65c&rfr_iscdi=true |