Development of rhesus macaque astrocyte cell lines supporting infection with a panel of viruses
Non-human primate (NHP)-based model systems are highly relevant for biomedical research. However, only few NHP cell lines are available and the generation of additional cell lines is an urgent need to help in the refinement and replacement of these models. Using lentiviral transduction of c-Fos, we...
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| creator | Reiter, Stefanie Sun, Ting Gärtner, Sabine Pöhlmann, Stefan Winkler, Michael |
| description | Non-human primate (NHP)-based model systems are highly relevant for biomedical research. However, only few NHP cell lines are available and the generation of additional cell lines is an urgent need to help in the refinement and replacement of these models. Using lentiviral transduction of c-Fos, we established cell lines from the brain of rhesus macaques (Macaca mulatta). Transcriptome analysis revealed that these cell lines are closely related to astrocytes, which was confirmed by immunoblot and immunofluorescence microscopy detecting expression of the astrocyte marker glial fibrillary acidic protein (GFAP). Quantitative real-time PCR (qRT-PCR) demonstrated that major pathways of the interferon (IFN) system are intact. Using retroviral pseudotypes we found that the cell lines are susceptible to entry driven by the glycoproteins of vesicular stomatitis virus (VSV), lymphocytic choriomeningitis virus (LCMV) and to a lesser extent influenza A virus (IAV). Finally, these cells supported growth of Zika virus (ZIKV) and Papiine alphaherpesvirus 2 (PaHV2). In summary, we developed IFN-responsive cell lines from the rhesus macaque brain that allowed entry driven by several viral glycoproteins and were permissive to infection with ZIKV and a primate simplexvirus. These cell lines will be useful for efforts to analyze neurotropic viral infections in rhesus macaque models. |
| doi_str_mv | 10.1371/journal.pone.0303059 |
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However, only few NHP cell lines are available and the generation of additional cell lines is an urgent need to help in the refinement and replacement of these models. Using lentiviral transduction of c-Fos, we established cell lines from the brain of rhesus macaques (Macaca mulatta). Transcriptome analysis revealed that these cell lines are closely related to astrocytes, which was confirmed by immunoblot and immunofluorescence microscopy detecting expression of the astrocyte marker glial fibrillary acidic protein (GFAP). Quantitative real-time PCR (qRT-PCR) demonstrated that major pathways of the interferon (IFN) system are intact. Using retroviral pseudotypes we found that the cell lines are susceptible to entry driven by the glycoproteins of vesicular stomatitis virus (VSV), lymphocytic choriomeningitis virus (LCMV) and to a lesser extent influenza A virus (IAV). Finally, these cells supported growth of Zika virus (ZIKV) and Papiine alphaherpesvirus 2 (PaHV2). In summary, we developed IFN-responsive cell lines from the rhesus macaque brain that allowed entry driven by several viral glycoproteins and were permissive to infection with ZIKV and a primate simplexvirus. These cell lines will be useful for efforts to analyze neurotropic viral infections in rhesus macaque models.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0303059</identifier><identifier>PMID: 38743751</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Amino acids ; Analysis ; Animal models ; Animals ; Astrocytes ; Astrocytes - metabolism ; Astrocytes - virology ; Biological response modifiers ; Biology and life sciences ; Brain ; Brain - metabolism ; Brain - virology ; c-Fos protein ; Cell culture ; Cell Line ; Cell lines ; Cloning ; Cytomegalovirus ; Ethylenediaminetetraacetic acid ; Genes ; Glial fibrillary acidic protein ; Glycoproteins ; Health aspects ; HIV ; Human immunodeficiency virus ; Humans ; Immunofluorescence ; Infections ; Infectious diseases ; Influenza A ; Interferon ; Macaca mulatta ; Medical research ; Medicine and Health Sciences ; Medicine, Experimental ; Monkeys & apes ; Neomycin ; Penicillin ; Plasmids ; Primates ; Real time ; Research and Analysis Methods ; Stomatitis ; Transcriptomes ; Vector-borne diseases ; Viral infections ; Viruses ; Zika virus</subject><ispartof>PloS one, 2024-05, Vol.19 (5), p.e0303059-e0303059</ispartof><rights>Copyright: © 2024 Reiter et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.</rights><rights>COPYRIGHT 2024 Public Library of Science</rights><rights>2024 Reiter et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2024 Reiter et al 2024 Reiter et al</rights><rights>2024 Reiter et al. 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These cell lines will be useful for efforts to analyze neurotropic viral infections in rhesus macaque models.</description><subject>Amino acids</subject><subject>Analysis</subject><subject>Animal models</subject><subject>Animals</subject><subject>Astrocytes</subject><subject>Astrocytes - metabolism</subject><subject>Astrocytes - virology</subject><subject>Biological response modifiers</subject><subject>Biology and life sciences</subject><subject>Brain</subject><subject>Brain - metabolism</subject><subject>Brain - virology</subject><subject>c-Fos protein</subject><subject>Cell culture</subject><subject>Cell Line</subject><subject>Cell lines</subject><subject>Cloning</subject><subject>Cytomegalovirus</subject><subject>Ethylenediaminetetraacetic acid</subject><subject>Genes</subject><subject>Glial fibrillary acidic protein</subject><subject>Glycoproteins</subject><subject>Health aspects</subject><subject>HIV</subject><subject>Human immunodeficiency 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In summary, we developed IFN-responsive cell lines from the rhesus macaque brain that allowed entry driven by several viral glycoproteins and were permissive to infection with ZIKV and a primate simplexvirus. These cell lines will be useful for efforts to analyze neurotropic viral infections in rhesus macaque models.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>38743751</pmid><doi>10.1371/journal.pone.0303059</doi><tpages>e0303059</tpages><orcidid>https://orcid.org/0000-0001-6086-9136</orcidid><orcidid>https://orcid.org/0000-0002-6198-554X</orcidid><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2024-05, Vol.19 (5), p.e0303059-e0303059 |
| issn | 1932-6203 1932-6203 |
| language | eng |
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| source | MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Public Library of Science (PLoS); PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Amino acids Analysis Animal models Animals Astrocytes Astrocytes - metabolism Astrocytes - virology Biological response modifiers Biology and life sciences Brain Brain - metabolism Brain - virology c-Fos protein Cell culture Cell Line Cell lines Cloning Cytomegalovirus Ethylenediaminetetraacetic acid Genes Glial fibrillary acidic protein Glycoproteins Health aspects HIV Human immunodeficiency virus Humans Immunofluorescence Infections Infectious diseases Influenza A Interferon Macaca mulatta Medical research Medicine and Health Sciences Medicine, Experimental Monkeys & apes Neomycin Penicillin Plasmids Primates Real time Research and Analysis Methods Stomatitis Transcriptomes Vector-borne diseases Viral infections Viruses Zika virus |
| title | Development of rhesus macaque astrocyte cell lines supporting infection with a panel of viruses |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-19T13%3A17%3A39IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Development%20of%20rhesus%20macaque%20astrocyte%20cell%20lines%20supporting%20infection%20with%20a%20panel%20of%20viruses&rft.jtitle=PloS%20one&rft.au=Reiter,%20Stefanie&rft.date=2024-05-14&rft.volume=19&rft.issue=5&rft.spage=e0303059&rft.epage=e0303059&rft.pages=e0303059-e0303059&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0303059&rft_dat=%3Cgale_plos_%3EA793817783%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=3069288151&rft_id=info:pmid/38743751&rft_galeid=A793817783&rft_doaj_id=oai_doaj_org_article_dac4eab56a984a2b89e19164147fae98&rfr_iscdi=true |