The clinical significance of long non-coding RNAs MALAT1 and CASC2 in the diagnosis of HCV-related hepatocellular carcinoma
Globally, hepatocellular carcinoma (HCC) is the second most common cause of cancer-related death due to a lack of early predictive and/or diagnostic tools. Thus, research for a new biomarker is important. LncRNAs play a functional role in target gene regulation and their deregulation is associated w...
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| Veröffentlicht in: | PloS one 2024-05, Vol.19 (5), p.e0303314-e0303314 |
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| creator | Golam, Rehab M Khalil, Mahmoud A F Shaker, Olfat G Ahmed, Tarek I Elguaad, Mohamed K Abd Hassan, Essam A El-Ansary, Mahmoud R M Ismail, Ahmed Kandil, Yasser I Mohammed, Osama A Doghish, Ahmed S |
| description | Globally, hepatocellular carcinoma (HCC) is the second most common cause of cancer-related death due to a lack of early predictive and/or diagnostic tools. Thus, research for a new biomarker is important. LncRNAs play a functional role in target gene regulation and their deregulation is associated with several pathological conditions including HCC.
This study aimed to explore the diagnostic potential of two LncRNAs MALAT1 and CASC2 in HCC compared to the routinely used diagnostic biomarker.
The current study is a case-control study carried out at Fayoum University Hospital and conducted on 89 individuals. The study included three groups of 36 HCC patients on top of HCV(HCC/HCV), 33 HCV patients, and 20 healthy volunteers as a control group. All study subjects were subjected to radiological examinations. The determination of CBC was performed by the automated counter and liver function tests by the enzymatic method were performed. In addition, HCV RNA quantification and the expression level of two LncRNAs (MALAT1 and CASC2) were performed by qRT-PCR.
The results revealed a statistically significant difference between study groups regarding liver function tests with a higher mean in HCC/HCV group. Also, serum MALAT1 significantly up-regulated in HCV (11.2±2.8) and HCC/HCV (4.56±1.4) compared to the control group. Besides, serum CASC2 levels in the HCV group were significantly upregulated (14.9±3.6), while, downregulated in the HCC group (0.16± 0.03). Furthermore, The ROC analysis for diagnostic efficacy parameters indicated that CASC2 has higher accuracy (94.6%) and sensitivity (97.2%) for HCC diagnosis than AFP with an accuracy of (90.9%), sensitivity (69.4%), and MALAT1 showed an accuracy of (56.9%), sensitivity (72.2%).
Our study results indicated that CASC2 is a promising biomarker and is considered better and could help in HCC diagnosis on top of HCV than MALAT1 and the routine biomarker AFP. |
| doi_str_mv | 10.1371/journal.pone.0303314 |
| format | Article |
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This study aimed to explore the diagnostic potential of two LncRNAs MALAT1 and CASC2 in HCC compared to the routinely used diagnostic biomarker.
The current study is a case-control study carried out at Fayoum University Hospital and conducted on 89 individuals. The study included three groups of 36 HCC patients on top of HCV(HCC/HCV), 33 HCV patients, and 20 healthy volunteers as a control group. All study subjects were subjected to radiological examinations. The determination of CBC was performed by the automated counter and liver function tests by the enzymatic method were performed. In addition, HCV RNA quantification and the expression level of two LncRNAs (MALAT1 and CASC2) were performed by qRT-PCR.
The results revealed a statistically significant difference between study groups regarding liver function tests with a higher mean in HCC/HCV group. Also, serum MALAT1 significantly up-regulated in HCV (11.2±2.8) and HCC/HCV (4.56±1.4) compared to the control group. Besides, serum CASC2 levels in the HCV group were significantly upregulated (14.9±3.6), while, downregulated in the HCC group (0.16± 0.03). Furthermore, The ROC analysis for diagnostic efficacy parameters indicated that CASC2 has higher accuracy (94.6%) and sensitivity (97.2%) for HCC diagnosis than AFP with an accuracy of (90.9%), sensitivity (69.4%), and MALAT1 showed an accuracy of (56.9%), sensitivity (72.2%).
Our study results indicated that CASC2 is a promising biomarker and is considered better and could help in HCC diagnosis on top of HCV than MALAT1 and the routine biomarker AFP.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0303314</identifier><identifier>PMID: 38739668</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Adult ; Aged ; Biology and life sciences ; Biomarkers ; Biomarkers, Tumor - blood ; Biomarkers, Tumor - genetics ; Blood ; Cancer ; Carcinoma, Hepatocellular - diagnosis ; Carcinoma, Hepatocellular - genetics ; Carcinoma, Hepatocellular - virology ; Case-Control Studies ; Clinical Relevance ; Deregulation ; Diagnosis ; Enzymes ; Ethylenediaminetetraacetic acid ; Female ; Females ; Gene expression ; Gene Expression Regulation, Neoplastic ; Gene regulation ; Genes ; Genetic aspects ; Health aspects ; Hemoglobin ; Hepacivirus - genetics ; Hepatitis B ; Hepatitis C - complications ; Hepatitis C - diagnosis ; Hepatitis C - genetics ; Hepatitis C - virology ; Hepatitis C virus ; Hepatocellular carcinoma ; Hepatoma ; Humans ; Infections ; Liver ; Liver cancer ; Liver Neoplasms - diagnosis ; Liver Neoplasms - genetics ; Liver Neoplasms - virology ; Male ; Medical diagnosis ; Medical research ; Medicine and Health Sciences ; MicroRNAs ; Middle Aged ; Non-coding RNA ; Parameter sensitivity ; Patients ; Phosphatase ; Proteins ; RNA ; RNA, Long Noncoding - blood ; RNA, Long Noncoding - genetics ; ROC Curve ; Sensitivity ; Statistical analysis ; Tumor Suppressor Proteins - genetics ; Ultrasonic imaging ; Variance analysis</subject><ispartof>PloS one, 2024-05, Vol.19 (5), p.e0303314-e0303314</ispartof><rights>Copyright: © 2024 Golam et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.</rights><rights>COPYRIGHT 2024 Public Library of Science</rights><rights>2024 Golam et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2024 Golam et al 2024 Golam et al</rights><rights>2024 Golam et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. 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Thus, research for a new biomarker is important. LncRNAs play a functional role in target gene regulation and their deregulation is associated with several pathological conditions including HCC.
This study aimed to explore the diagnostic potential of two LncRNAs MALAT1 and CASC2 in HCC compared to the routinely used diagnostic biomarker.
The current study is a case-control study carried out at Fayoum University Hospital and conducted on 89 individuals. The study included three groups of 36 HCC patients on top of HCV(HCC/HCV), 33 HCV patients, and 20 healthy volunteers as a control group. All study subjects were subjected to radiological examinations. The determination of CBC was performed by the automated counter and liver function tests by the enzymatic method were performed. In addition, HCV RNA quantification and the expression level of two LncRNAs (MALAT1 and CASC2) were performed by qRT-PCR.
The results revealed a statistically significant difference between study groups regarding liver function tests with a higher mean in HCC/HCV group. Also, serum MALAT1 significantly up-regulated in HCV (11.2±2.8) and HCC/HCV (4.56±1.4) compared to the control group. Besides, serum CASC2 levels in the HCV group were significantly upregulated (14.9±3.6), while, downregulated in the HCC group (0.16± 0.03). Furthermore, The ROC analysis for diagnostic efficacy parameters indicated that CASC2 has higher accuracy (94.6%) and sensitivity (97.2%) for HCC diagnosis than AFP with an accuracy of (90.9%), sensitivity (69.4%), and MALAT1 showed an accuracy of (56.9%), sensitivity (72.2%).
Our study results indicated that CASC2 is a promising biomarker and is considered better and could help in HCC diagnosis on top of HCV than MALAT1 and the routine biomarker AFP.</description><subject>Adult</subject><subject>Aged</subject><subject>Biology and life sciences</subject><subject>Biomarkers</subject><subject>Biomarkers, Tumor - blood</subject><subject>Biomarkers, Tumor - genetics</subject><subject>Blood</subject><subject>Cancer</subject><subject>Carcinoma, Hepatocellular - diagnosis</subject><subject>Carcinoma, Hepatocellular - genetics</subject><subject>Carcinoma, Hepatocellular - virology</subject><subject>Case-Control Studies</subject><subject>Clinical Relevance</subject><subject>Deregulation</subject><subject>Diagnosis</subject><subject>Enzymes</subject><subject>Ethylenediaminetetraacetic acid</subject><subject>Female</subject><subject>Females</subject><subject>Gene expression</subject><subject>Gene Expression Regulation, Neoplastic</subject><subject>Gene regulation</subject><subject>Genes</subject><subject>Genetic aspects</subject><subject>Health aspects</subject><subject>Hemoglobin</subject><subject>Hepacivirus - 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diagnosis</topic><topic>Liver Neoplasms - genetics</topic><topic>Liver Neoplasms - virology</topic><topic>Male</topic><topic>Medical diagnosis</topic><topic>Medical research</topic><topic>Medicine and Health Sciences</topic><topic>MicroRNAs</topic><topic>Middle Aged</topic><topic>Non-coding RNA</topic><topic>Parameter sensitivity</topic><topic>Patients</topic><topic>Phosphatase</topic><topic>Proteins</topic><topic>RNA</topic><topic>RNA, Long Noncoding - blood</topic><topic>RNA, Long Noncoding - genetics</topic><topic>ROC Curve</topic><topic>Sensitivity</topic><topic>Statistical analysis</topic><topic>Tumor Suppressor Proteins - genetics</topic><topic>Ultrasonic imaging</topic><topic>Variance analysis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Golam, Rehab M</creatorcontrib><creatorcontrib>Khalil, Mahmoud A F</creatorcontrib><creatorcontrib>Shaker, Olfat G</creatorcontrib><creatorcontrib>Ahmed, Tarek I</creatorcontrib><creatorcontrib>Elguaad, Mohamed K Abd</creatorcontrib><creatorcontrib>Hassan, Essam A</creatorcontrib><creatorcontrib>El-Ansary, Mahmoud R M</creatorcontrib><creatorcontrib>Ismail, Ahmed</creatorcontrib><creatorcontrib>Kandil, Yasser I</creatorcontrib><creatorcontrib>Mohammed, Osama A</creatorcontrib><creatorcontrib>Doghish, Ahmed S</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: Opposing Viewpoints</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Nursing & Allied Health Database</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Meteorological & Geoastrophysical Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Meteorological & Geoastrophysical Abstracts - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Golam, Rehab M</au><au>Khalil, Mahmoud A F</au><au>Shaker, Olfat G</au><au>Ahmed, Tarek I</au><au>Elguaad, Mohamed K Abd</au><au>Hassan, Essam A</au><au>El-Ansary, Mahmoud R M</au><au>Ismail, Ahmed</au><au>Kandil, Yasser I</au><au>Mohammed, Osama A</au><au>Doghish, Ahmed S</au><au>Bernardes de Jesus, Bruno</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The clinical significance of long non-coding RNAs MALAT1 and CASC2 in the diagnosis of HCV-related hepatocellular carcinoma</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2024-05-13</date><risdate>2024</risdate><volume>19</volume><issue>5</issue><spage>e0303314</spage><epage>e0303314</epage><pages>e0303314-e0303314</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Globally, hepatocellular carcinoma (HCC) is the second most common cause of cancer-related death due to a lack of early predictive and/or diagnostic tools. Thus, research for a new biomarker is important. LncRNAs play a functional role in target gene regulation and their deregulation is associated with several pathological conditions including HCC.
This study aimed to explore the diagnostic potential of two LncRNAs MALAT1 and CASC2 in HCC compared to the routinely used diagnostic biomarker.
The current study is a case-control study carried out at Fayoum University Hospital and conducted on 89 individuals. The study included three groups of 36 HCC patients on top of HCV(HCC/HCV), 33 HCV patients, and 20 healthy volunteers as a control group. All study subjects were subjected to radiological examinations. The determination of CBC was performed by the automated counter and liver function tests by the enzymatic method were performed. In addition, HCV RNA quantification and the expression level of two LncRNAs (MALAT1 and CASC2) were performed by qRT-PCR.
The results revealed a statistically significant difference between study groups regarding liver function tests with a higher mean in HCC/HCV group. Also, serum MALAT1 significantly up-regulated in HCV (11.2±2.8) and HCC/HCV (4.56±1.4) compared to the control group. Besides, serum CASC2 levels in the HCV group were significantly upregulated (14.9±3.6), while, downregulated in the HCC group (0.16± 0.03). Furthermore, The ROC analysis for diagnostic efficacy parameters indicated that CASC2 has higher accuracy (94.6%) and sensitivity (97.2%) for HCC diagnosis than AFP with an accuracy of (90.9%), sensitivity (69.4%), and MALAT1 showed an accuracy of (56.9%), sensitivity (72.2%).
Our study results indicated that CASC2 is a promising biomarker and is considered better and could help in HCC diagnosis on top of HCV than MALAT1 and the routine biomarker AFP.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>38739668</pmid><doi>10.1371/journal.pone.0303314</doi><tpages>e0303314</tpages><orcidid>https://orcid.org/0000-0002-0136-7096</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2024-05, Vol.19 (5), p.e0303314-e0303314 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_3069288140 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Public Library of Science (PLoS); PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Adult Aged Biology and life sciences Biomarkers Biomarkers, Tumor - blood Biomarkers, Tumor - genetics Blood Cancer Carcinoma, Hepatocellular - diagnosis Carcinoma, Hepatocellular - genetics Carcinoma, Hepatocellular - virology Case-Control Studies Clinical Relevance Deregulation Diagnosis Enzymes Ethylenediaminetetraacetic acid Female Females Gene expression Gene Expression Regulation, Neoplastic Gene regulation Genes Genetic aspects Health aspects Hemoglobin Hepacivirus - genetics Hepatitis B Hepatitis C - complications Hepatitis C - diagnosis Hepatitis C - genetics Hepatitis C - virology Hepatitis C virus Hepatocellular carcinoma Hepatoma Humans Infections Liver Liver cancer Liver Neoplasms - diagnosis Liver Neoplasms - genetics Liver Neoplasms - virology Male Medical diagnosis Medical research Medicine and Health Sciences MicroRNAs Middle Aged Non-coding RNA Parameter sensitivity Patients Phosphatase Proteins RNA RNA, Long Noncoding - blood RNA, Long Noncoding - genetics ROC Curve Sensitivity Statistical analysis Tumor Suppressor Proteins - genetics Ultrasonic imaging Variance analysis |
| title | The clinical significance of long non-coding RNAs MALAT1 and CASC2 in the diagnosis of HCV-related hepatocellular carcinoma |
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