Flow cytometry-based quantification of genome editing efficiency in human cell lines using the L1CAM gene

CRISPR/Cas9 is a powerful genome editing system that has remarkably facilitated gene knockout and targeted knock-in. To accelerate the practical use of CRISPR/Cas9, however, it remains crucial to improve the efficiency, precision, and specificity of genome editing, particularly targeted knock-in, ac...

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Veröffentlicht in:PloS one 2023-11, Vol.18 (11), p.e0294146-e0294146
Hauptverfasser: Hasan, Muhammad Nazmul, Hyodo, Toshinori, Biswas, Mrityunjoy, Rahman, Md. Lutfur, Mihara, Yuko, Karnan, Sivasundaram, Ota, Akinobu, Tsuzuki, Shinobu, Hosokawa, Yoshitaka, Konishi, Hiroyuki
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Sprache:eng
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