Bacterial production of ciprofloxacin and potential usage as a radiotracer
Infectious diseases caused by bacteria that have become resistant to antibiotics have increased in prevalence, necessitating new methods for their diagnosis and treatment. The aim of this study was to compare the efficacy of synthetic ciprofloxacin to that of organic ciprofloxacin produced by cave m...
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| creator | Karatay, Kadriye Busra Dogruoz Gungor, Nihal Colak, Batu Biber Muftuler, Fazilet Zumrut Aras, Omer |
| description | Infectious diseases caused by bacteria that have become resistant to antibiotics have increased in prevalence, necessitating new methods for their diagnosis and treatment. The aim of this study was to compare the efficacy of synthetic ciprofloxacin to that of organic ciprofloxacin produced by cave microorganisms, as well as to evaluate the feasibility of using organic ciprofloxacin radiolabeled with technetium-99m as an imaging agent. Organic ciprofloxacin produced by cave bacteria isolated from sediment taken from the dark zone of Antalya's "Yark Sinkhole," (Turkey's 14th deepest cave), was purified using high-performance liquid chromatography. Purified organic ciprofloxacin and standard ciprofloxacin were radiolabeled with technetium-99m (99mTc), and their uptake by pathogenic microorganisms as well as potential as an imaging agent were examined. According to thin-layer radiochromatography, radiolabeling efficiencies were 98.99 ± 0.34 (n = 7) and 91.25 ± 1.84 (n = 7) for radiolabeled organic ciprofloxacin and standard ciprofloxacin respectively. The binding efficiency of radiolabeled organic ciprofloxacin at the 240th minute was higher compared with radiolabeled standard ciprofloxacin, especially with P.aeruginosa, MRSA, VRE and E.coli. The results demonstrate that radiolabeling with 99mTc does not alter the biological behavior of organic ciprofloxacin, and radiolabeled organic ciprofloxacin has potential as an imaging agent for the detection of bacterial infection. The original value of the study is the monitoring of the antibiofilm effects of untouched cave-derived organic antibiotics by radiolabeling with a radionuclide. |
| doi_str_mv | 10.1371/journal.pone.0291342 |
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The aim of this study was to compare the efficacy of synthetic ciprofloxacin to that of organic ciprofloxacin produced by cave microorganisms, as well as to evaluate the feasibility of using organic ciprofloxacin radiolabeled with technetium-99m as an imaging agent. Organic ciprofloxacin produced by cave bacteria isolated from sediment taken from the dark zone of Antalya's "Yark Sinkhole," (Turkey's 14th deepest cave), was purified using high-performance liquid chromatography. Purified organic ciprofloxacin and standard ciprofloxacin were radiolabeled with technetium-99m (99mTc), and their uptake by pathogenic microorganisms as well as potential as an imaging agent were examined. According to thin-layer radiochromatography, radiolabeling efficiencies were 98.99 ± 0.34 (n = 7) and 91.25 ± 1.84 (n = 7) for radiolabeled organic ciprofloxacin and standard ciprofloxacin respectively. The binding efficiency of radiolabeled organic ciprofloxacin at the 240th minute was higher compared with radiolabeled standard ciprofloxacin, especially with P.aeruginosa, MRSA, VRE and E.coli. The results demonstrate that radiolabeling with 99mTc does not alter the biological behavior of organic ciprofloxacin, and radiolabeled organic ciprofloxacin has potential as an imaging agent for the detection of bacterial infection. The original value of the study is the monitoring of the antibiofilm effects of untouched cave-derived organic antibiotics by radiolabeling with a radionuclide.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0291342</identifier><identifier>PMID: 37943851</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Anti-Bacterial Agents - pharmacology ; Antibiotics ; Antimicrobial agents ; Bacteria ; Bacterial diseases ; Bacterial infections ; Biology and Life Sciences ; Care and treatment ; Chromatography ; Ciprofloxacin ; Ciprofloxacin - chemistry ; Ciprofloxacin - pharmacology ; Diagnosis ; Drug resistance ; E coli ; Escherichia coli - metabolism ; Evaluation ; Gas flow ; High performance liquid chromatography ; Infectious diseases ; Liquid chromatography ; Medical diagnosis ; Medical imaging ; Medical research ; Medicine and Health Sciences ; Methicillin ; Methods ; Microorganisms ; Nuclear medicine ; Peptides ; Properties ; Radioactive tracers ; Radioisotopes ; Radiolabelling ; Radionuclide Imaging ; Radiopharmaceuticals - chemistry ; Research and Analysis Methods ; Sinkholes ; Staphylococcus infections ; Technetium ; Technetium - chemistry ; Technetium isotopes</subject><ispartof>PloS one, 2023-11, Vol.18 (11), p.e0291342-e0291342</ispartof><rights>Copyright: © 2023 Karatay et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.</rights><rights>COPYRIGHT 2023 Public Library of Science</rights><rights>2023 Karatay et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2023 Karatay et al 2023 Karatay et al</rights><rights>2023 Karatay et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. 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The binding efficiency of radiolabeled organic ciprofloxacin at the 240th minute was higher compared with radiolabeled standard ciprofloxacin, especially with P.aeruginosa, MRSA, VRE and E.coli. The results demonstrate that radiolabeling with 99mTc does not alter the biological behavior of organic ciprofloxacin, and radiolabeled organic ciprofloxacin has potential as an imaging agent for the detection of bacterial infection. 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The aim of this study was to compare the efficacy of synthetic ciprofloxacin to that of organic ciprofloxacin produced by cave microorganisms, as well as to evaluate the feasibility of using organic ciprofloxacin radiolabeled with technetium-99m as an imaging agent. Organic ciprofloxacin produced by cave bacteria isolated from sediment taken from the dark zone of Antalya's "Yark Sinkhole," (Turkey's 14th deepest cave), was purified using high-performance liquid chromatography. Purified organic ciprofloxacin and standard ciprofloxacin were radiolabeled with technetium-99m (99mTc), and their uptake by pathogenic microorganisms as well as potential as an imaging agent were examined. According to thin-layer radiochromatography, radiolabeling efficiencies were 98.99 ± 0.34 (n = 7) and 91.25 ± 1.84 (n = 7) for radiolabeled organic ciprofloxacin and standard ciprofloxacin respectively. The binding efficiency of radiolabeled organic ciprofloxacin at the 240th minute was higher compared with radiolabeled standard ciprofloxacin, especially with P.aeruginosa, MRSA, VRE and E.coli. The results demonstrate that radiolabeling with 99mTc does not alter the biological behavior of organic ciprofloxacin, and radiolabeled organic ciprofloxacin has potential as an imaging agent for the detection of bacterial infection. The original value of the study is the monitoring of the antibiofilm effects of untouched cave-derived organic antibiotics by radiolabeling with a radionuclide.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>37943851</pmid><doi>10.1371/journal.pone.0291342</doi><tpages>e0291342</tpages><orcidid>https://orcid.org/0000-0001-8184-9660</orcidid><orcidid>https://orcid.org/0000-0003-1758-0542</orcidid><oa>free_for_read</oa></addata></record> |
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| ispartof | PloS one, 2023-11, Vol.18 (11), p.e0291342-e0291342 |
| issn | 1932-6203 1932-6203 |
| language | eng |
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| source | MEDLINE; DOAJ Directory of Open Access Journals; Public Library of Science (PLoS); EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Anti-Bacterial Agents - pharmacology Antibiotics Antimicrobial agents Bacteria Bacterial diseases Bacterial infections Biology and Life Sciences Care and treatment Chromatography Ciprofloxacin Ciprofloxacin - chemistry Ciprofloxacin - pharmacology Diagnosis Drug resistance E coli Escherichia coli - metabolism Evaluation Gas flow High performance liquid chromatography Infectious diseases Liquid chromatography Medical diagnosis Medical imaging Medical research Medicine and Health Sciences Methicillin Methods Microorganisms Nuclear medicine Peptides Properties Radioactive tracers Radioisotopes Radiolabelling Radionuclide Imaging Radiopharmaceuticals - chemistry Research and Analysis Methods Sinkholes Staphylococcus infections Technetium Technetium - chemistry Technetium isotopes |
| title | Bacterial production of ciprofloxacin and potential usage as a radiotracer |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-22T13%3A56%3A16IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Bacterial%20production%20of%20ciprofloxacin%20and%20potential%20usage%20as%20a%20radiotracer&rft.jtitle=PloS%20one&rft.au=Karatay,%20Kadriye%20Busra&rft.date=2023-11-09&rft.volume=18&rft.issue=11&rft.spage=e0291342&rft.epage=e0291342&rft.pages=e0291342-e0291342&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0291342&rft_dat=%3Cgale_plos_%3EA772190661%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=3069280837&rft_id=info:pmid/37943851&rft_galeid=A772190661&rft_doaj_id=oai_doaj_org_article_e170aece6f354d99b51e1da8ccf1b530&rfr_iscdi=true |