Promotion of Th1 and Th2 responses over Th17 in Riemerella anatipestifer stimulation in chicken splenocytes: Correlation of gga-miR-456-3p and gga-miR-16-5p with NOS2 and CCL5 expression
Riemerella (R.) anatipestifer poses a significant threat to ducks, resulting in mortality rates ranging from 5-75%. This disease is highly infectious and economically consequential for domestic ducks. Although other avian species, such as chickens, also display susceptibility, the impact is comparat...
Gespeichert in:
| Veröffentlicht in: | PloS one 2023-11, Vol.18 (11), p.e0294031-e0294031 |
|---|---|
| Hauptverfasser: | , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | e0294031 |
|---|---|
| container_issue | 11 |
| container_start_page | e0294031 |
| container_title | PloS one |
| container_volume | 18 |
| creator | Cammayo-Fletcher, Paula Leona T Flores, Rochelle A Nguyen, Binh T Villavicencio, Andrea Gail M Lee, Seung Yun Kim, Woo H Min, Wongi |
| description | Riemerella (R.) anatipestifer poses a significant threat to ducks, resulting in mortality rates ranging from 5-75%. This disease is highly infectious and economically consequential for domestic ducks. Although other avian species, such as chickens, also display susceptibility, the impact is comparatively less severe than in ducks. IL-17A has a pronounced correlation with R. anatipestifer infection in ducks, which is less in chickens. This study performed an in vitro transcriptome analysis using chicken splenic lymphocytes collected at 4-, 8-, and 24-hour intervals following R. anatipestifer stimulation. The primary objective was to discern the differentially expressed genes, with a specific focus on IL-17A and IL-17F expression. Moreover, an association between specific miRNAs with NOS2 and CCL5 was identified. The manifestation of riemerellosis in chickens was linked to heightened expression of Th1- and Th2-associated cells, while Th17 cells exhibited minimal involvement. This study elucidated the mechanism behind the absence of a Th17 immune response, shedding light on its role throughout disease progression. Additionally, through small RNA sequencing, we identified a connection between miRNAs, specifically miR-456-3p and miR-16-5p, and their respective target genes NOS2 and CCL5. These miRNAs are potential regulators of the inflammatory process during riemerellosis in chickens. |
| doi_str_mv | 10.1371/journal.pone.0294031 |
| format | Article |
| fullrecord | <record><control><sourceid>gale_plos_</sourceid><recordid>TN_cdi_plos_journals_3069280550</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A771800362</galeid><doaj_id>oai_doaj_org_article_c20ee8cc0cee486985523773179c162d</doaj_id><sourcerecordid>A771800362</sourcerecordid><originalsourceid>FETCH-LOGICAL-c670t-a3206a0899f2e4bf12919ecbda136ddaf4010782161dbab67ac37a66a1ee1b533</originalsourceid><addsrcrecordid>eNqNk9uO0zAQhiMEEkvhDZCIhITgIsWHxE64QauKQ6WKou7CreU4k8RLEgc7WXZfjafDaVO0RXuBcjHRzDf_79MEwXOMlphy_PbKjLaTzbI3HSwRyWJE8YPgDGeURIwg-vDO_-PgiXNXCCU0Zews-P3VmtYM2nShKcPLGoeyK3wkoQXn9Ry40FyDnUo81F2409CChaaRnpSD7sENuvSAD-3YyL2U51St1Q_oQtc30Bl1O4B7F66M9a3yaFdVMmr1LooTFtF-73xMYRYlffhLD3X4ZXtB9rXVapOEcNP7lTmv8DR4VMrGwbM5LoJvHz9crj5Hm-2n9ep8EynG0RBJShCTKM2ykkCcl5hkOAOVFxJTVhSyjBFGPCWY4SKXOeNSUS4ZkxgA5wmli-DFQbdvjBPzWTtBEctIipIEeWJ9IAojr0RvdSvtrTBSi33C2EpIO2jVgFAEAaRKIQUQpyxLk4RQzinmmcKMFF7r_ew25i0UCrrByuZE9LTS6VpU5lpgxAiPk8wrvJ4VrPk5-usRrXZqurEOzOgESb2v9_Sui-DlP-j925upSvod6K403lhNouKcc5wiRBnx1PIeyn8FtFr5l1lqnz9peHPS4JkBboZKjs6J9cXu_9nt91P21R22BtkMtTPNOL06dwrGB1BZ45yF8u8pYySmwTqehpgGS8yDRf8AQ0IU8Q</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>3069280550</pqid></control><display><type>article</type><title>Promotion of Th1 and Th2 responses over Th17 in Riemerella anatipestifer stimulation in chicken splenocytes: Correlation of gga-miR-456-3p and gga-miR-16-5p with NOS2 and CCL5 expression</title><source>DOAJ Directory of Open Access Journals</source><source>EZB-FREE-00999 freely available EZB journals</source><source>PubMed Central</source><source>Free Full-Text Journals in Chemistry</source><source>Public Library of Science (PLoS)</source><creator>Cammayo-Fletcher, Paula Leona T ; Flores, Rochelle A ; Nguyen, Binh T ; Villavicencio, Andrea Gail M ; Lee, Seung Yun ; Kim, Woo H ; Min, Wongi</creator><creatorcontrib>Cammayo-Fletcher, Paula Leona T ; Flores, Rochelle A ; Nguyen, Binh T ; Villavicencio, Andrea Gail M ; Lee, Seung Yun ; Kim, Woo H ; Min, Wongi</creatorcontrib><description>Riemerella (R.) anatipestifer poses a significant threat to ducks, resulting in mortality rates ranging from 5-75%. This disease is highly infectious and economically consequential for domestic ducks. Although other avian species, such as chickens, also display susceptibility, the impact is comparatively less severe than in ducks. IL-17A has a pronounced correlation with R. anatipestifer infection in ducks, which is less in chickens. This study performed an in vitro transcriptome analysis using chicken splenic lymphocytes collected at 4-, 8-, and 24-hour intervals following R. anatipestifer stimulation. The primary objective was to discern the differentially expressed genes, with a specific focus on IL-17A and IL-17F expression. Moreover, an association between specific miRNAs with NOS2 and CCL5 was identified. The manifestation of riemerellosis in chickens was linked to heightened expression of Th1- and Th2-associated cells, while Th17 cells exhibited minimal involvement. This study elucidated the mechanism behind the absence of a Th17 immune response, shedding light on its role throughout disease progression. Additionally, through small RNA sequencing, we identified a connection between miRNAs, specifically miR-456-3p and miR-16-5p, and their respective target genes NOS2 and CCL5. These miRNAs are potential regulators of the inflammatory process during riemerellosis in chickens.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0294031</identifier><language>eng</language><publisher>San Francisco: Public Library of Science</publisher><subject>Analysis ; Aquatic birds ; Bacteria ; Bacterial infections ; Biology and life sciences ; Chickens ; Cytokines ; Data analysis ; Development and progression ; Ducks ; Gene expression ; Gene sequencing ; Genes ; Genomes ; Health aspects ; Helper cells ; Immune response ; Infection ; Infections ; Inflammation ; Inflammatory diseases ; Lymphocytes ; Lymphocytes T ; Medicine and Health Sciences ; MicroRNA ; MicroRNAs ; Poultry ; Riemerella anatipestifer ; RNA sequencing ; Splenocytes ; Stimulation ; Transcriptomes ; Waterfowl</subject><ispartof>PloS one, 2023-11, Vol.18 (11), p.e0294031-e0294031</ispartof><rights>COPYRIGHT 2023 Public Library of Science</rights><rights>2023 Cammayo-Fletcher et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2023 Cammayo-Fletcher et al 2023 Cammayo-Fletcher et al</rights><rights>2023 Cammayo-Fletcher et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c670t-a3206a0899f2e4bf12919ecbda136ddaf4010782161dbab67ac37a66a1ee1b533</citedby><cites>FETCH-LOGICAL-c670t-a3206a0899f2e4bf12919ecbda136ddaf4010782161dbab67ac37a66a1ee1b533</cites><orcidid>0000-0003-2437-7366 ; 0000-0002-6874-6787</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC10627459/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC10627459/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,2096,2915,23845,27901,27902,53766,53768,79342,79343</link.rule.ids></links><search><creatorcontrib>Cammayo-Fletcher, Paula Leona T</creatorcontrib><creatorcontrib>Flores, Rochelle A</creatorcontrib><creatorcontrib>Nguyen, Binh T</creatorcontrib><creatorcontrib>Villavicencio, Andrea Gail M</creatorcontrib><creatorcontrib>Lee, Seung Yun</creatorcontrib><creatorcontrib>Kim, Woo H</creatorcontrib><creatorcontrib>Min, Wongi</creatorcontrib><title>Promotion of Th1 and Th2 responses over Th17 in Riemerella anatipestifer stimulation in chicken splenocytes: Correlation of gga-miR-456-3p and gga-miR-16-5p with NOS2 and CCL5 expression</title><title>PloS one</title><description>Riemerella (R.) anatipestifer poses a significant threat to ducks, resulting in mortality rates ranging from 5-75%. This disease is highly infectious and economically consequential for domestic ducks. Although other avian species, such as chickens, also display susceptibility, the impact is comparatively less severe than in ducks. IL-17A has a pronounced correlation with R. anatipestifer infection in ducks, which is less in chickens. This study performed an in vitro transcriptome analysis using chicken splenic lymphocytes collected at 4-, 8-, and 24-hour intervals following R. anatipestifer stimulation. The primary objective was to discern the differentially expressed genes, with a specific focus on IL-17A and IL-17F expression. Moreover, an association between specific miRNAs with NOS2 and CCL5 was identified. The manifestation of riemerellosis in chickens was linked to heightened expression of Th1- and Th2-associated cells, while Th17 cells exhibited minimal involvement. This study elucidated the mechanism behind the absence of a Th17 immune response, shedding light on its role throughout disease progression. Additionally, through small RNA sequencing, we identified a connection between miRNAs, specifically miR-456-3p and miR-16-5p, and their respective target genes NOS2 and CCL5. These miRNAs are potential regulators of the inflammatory process during riemerellosis in chickens.</description><subject>Analysis</subject><subject>Aquatic birds</subject><subject>Bacteria</subject><subject>Bacterial infections</subject><subject>Biology and life sciences</subject><subject>Chickens</subject><subject>Cytokines</subject><subject>Data analysis</subject><subject>Development and progression</subject><subject>Ducks</subject><subject>Gene expression</subject><subject>Gene sequencing</subject><subject>Genes</subject><subject>Genomes</subject><subject>Health aspects</subject><subject>Helper cells</subject><subject>Immune response</subject><subject>Infection</subject><subject>Infections</subject><subject>Inflammation</subject><subject>Inflammatory diseases</subject><subject>Lymphocytes</subject><subject>Lymphocytes T</subject><subject>Medicine and Health Sciences</subject><subject>MicroRNA</subject><subject>MicroRNAs</subject><subject>Poultry</subject><subject>Riemerella anatipestifer</subject><subject>RNA sequencing</subject><subject>Splenocytes</subject><subject>Stimulation</subject><subject>Transcriptomes</subject><subject>Waterfowl</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>BENPR</sourceid><sourceid>DOA</sourceid><recordid>eNqNk9uO0zAQhiMEEkvhDZCIhITgIsWHxE64QauKQ6WKou7CreU4k8RLEgc7WXZfjafDaVO0RXuBcjHRzDf_79MEwXOMlphy_PbKjLaTzbI3HSwRyWJE8YPgDGeURIwg-vDO_-PgiXNXCCU0Zews-P3VmtYM2nShKcPLGoeyK3wkoQXn9Ry40FyDnUo81F2409CChaaRnpSD7sENuvSAD-3YyL2U51St1Q_oQtc30Bl1O4B7F66M9a3yaFdVMmr1LooTFtF-73xMYRYlffhLD3X4ZXtB9rXVapOEcNP7lTmv8DR4VMrGwbM5LoJvHz9crj5Hm-2n9ep8EynG0RBJShCTKM2ykkCcl5hkOAOVFxJTVhSyjBFGPCWY4SKXOeNSUS4ZkxgA5wmli-DFQbdvjBPzWTtBEctIipIEeWJ9IAojr0RvdSvtrTBSi33C2EpIO2jVgFAEAaRKIQUQpyxLk4RQzinmmcKMFF7r_ew25i0UCrrByuZE9LTS6VpU5lpgxAiPk8wrvJ4VrPk5-usRrXZqurEOzOgESb2v9_Sui-DlP-j925upSvod6K403lhNouKcc5wiRBnx1PIeyn8FtFr5l1lqnz9peHPS4JkBboZKjs6J9cXu_9nt91P21R22BtkMtTPNOL06dwrGB1BZ45yF8u8pYySmwTqehpgGS8yDRf8AQ0IU8Q</recordid><startdate>20231106</startdate><enddate>20231106</enddate><creator>Cammayo-Fletcher, Paula Leona T</creator><creator>Flores, Rochelle A</creator><creator>Nguyen, Binh T</creator><creator>Villavicencio, Andrea Gail M</creator><creator>Lee, Seung Yun</creator><creator>Kim, Woo H</creator><creator>Min, Wongi</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0003-2437-7366</orcidid><orcidid>https://orcid.org/0000-0002-6874-6787</orcidid></search><sort><creationdate>20231106</creationdate><title>Promotion of Th1 and Th2 responses over Th17 in Riemerella anatipestifer stimulation in chicken splenocytes: Correlation of gga-miR-456-3p and gga-miR-16-5p with NOS2 and CCL5 expression</title><author>Cammayo-Fletcher, Paula Leona T ; Flores, Rochelle A ; Nguyen, Binh T ; Villavicencio, Andrea Gail M ; Lee, Seung Yun ; Kim, Woo H ; Min, Wongi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c670t-a3206a0899f2e4bf12919ecbda136ddaf4010782161dbab67ac37a66a1ee1b533</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Analysis</topic><topic>Aquatic birds</topic><topic>Bacteria</topic><topic>Bacterial infections</topic><topic>Biology and life sciences</topic><topic>Chickens</topic><topic>Cytokines</topic><topic>Data analysis</topic><topic>Development and progression</topic><topic>Ducks</topic><topic>Gene expression</topic><topic>Gene sequencing</topic><topic>Genes</topic><topic>Genomes</topic><topic>Health aspects</topic><topic>Helper cells</topic><topic>Immune response</topic><topic>Infection</topic><topic>Infections</topic><topic>Inflammation</topic><topic>Inflammatory diseases</topic><topic>Lymphocytes</topic><topic>Lymphocytes T</topic><topic>Medicine and Health Sciences</topic><topic>MicroRNA</topic><topic>MicroRNAs</topic><topic>Poultry</topic><topic>Riemerella anatipestifer</topic><topic>RNA sequencing</topic><topic>Splenocytes</topic><topic>Stimulation</topic><topic>Transcriptomes</topic><topic>Waterfowl</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Cammayo-Fletcher, Paula Leona T</creatorcontrib><creatorcontrib>Flores, Rochelle A</creatorcontrib><creatorcontrib>Nguyen, Binh T</creatorcontrib><creatorcontrib>Villavicencio, Andrea Gail M</creatorcontrib><creatorcontrib>Lee, Seung Yun</creatorcontrib><creatorcontrib>Kim, Woo H</creatorcontrib><creatorcontrib>Min, Wongi</creatorcontrib><collection>CrossRef</collection><collection>Gale In Context: Opposing Viewpoints</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Nursing & Allied Health Database</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Meteorological & Geoastrophysical Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Meteorological & Geoastrophysical Abstracts - Academic</collection><collection>ProQuest Engineering Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Engineering Database</collection><collection>Nursing & Allied Health Premium</collection><collection>Advanced Technologies & Aerospace Database</collection><collection>ProQuest Advanced Technologies & Aerospace Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>Materials Science Collection</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Engineering Collection</collection><collection>Environmental Science Collection</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Cammayo-Fletcher, Paula Leona T</au><au>Flores, Rochelle A</au><au>Nguyen, Binh T</au><au>Villavicencio, Andrea Gail M</au><au>Lee, Seung Yun</au><au>Kim, Woo H</au><au>Min, Wongi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Promotion of Th1 and Th2 responses over Th17 in Riemerella anatipestifer stimulation in chicken splenocytes: Correlation of gga-miR-456-3p and gga-miR-16-5p with NOS2 and CCL5 expression</atitle><jtitle>PloS one</jtitle><date>2023-11-06</date><risdate>2023</risdate><volume>18</volume><issue>11</issue><spage>e0294031</spage><epage>e0294031</epage><pages>e0294031-e0294031</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Riemerella (R.) anatipestifer poses a significant threat to ducks, resulting in mortality rates ranging from 5-75%. This disease is highly infectious and economically consequential for domestic ducks. Although other avian species, such as chickens, also display susceptibility, the impact is comparatively less severe than in ducks. IL-17A has a pronounced correlation with R. anatipestifer infection in ducks, which is less in chickens. This study performed an in vitro transcriptome analysis using chicken splenic lymphocytes collected at 4-, 8-, and 24-hour intervals following R. anatipestifer stimulation. The primary objective was to discern the differentially expressed genes, with a specific focus on IL-17A and IL-17F expression. Moreover, an association between specific miRNAs with NOS2 and CCL5 was identified. The manifestation of riemerellosis in chickens was linked to heightened expression of Th1- and Th2-associated cells, while Th17 cells exhibited minimal involvement. This study elucidated the mechanism behind the absence of a Th17 immune response, shedding light on its role throughout disease progression. Additionally, through small RNA sequencing, we identified a connection between miRNAs, specifically miR-456-3p and miR-16-5p, and their respective target genes NOS2 and CCL5. These miRNAs are potential regulators of the inflammatory process during riemerellosis in chickens.</abstract><cop>San Francisco</cop><pub>Public Library of Science</pub><doi>10.1371/journal.pone.0294031</doi><tpages>e0294031</tpages><orcidid>https://orcid.org/0000-0003-2437-7366</orcidid><orcidid>https://orcid.org/0000-0002-6874-6787</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2023-11, Vol.18 (11), p.e0294031-e0294031 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_3069280550 |
| source | DOAJ Directory of Open Access Journals; EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry; Public Library of Science (PLoS) |
| subjects | Analysis Aquatic birds Bacteria Bacterial infections Biology and life sciences Chickens Cytokines Data analysis Development and progression Ducks Gene expression Gene sequencing Genes Genomes Health aspects Helper cells Immune response Infection Infections Inflammation Inflammatory diseases Lymphocytes Lymphocytes T Medicine and Health Sciences MicroRNA MicroRNAs Poultry Riemerella anatipestifer RNA sequencing Splenocytes Stimulation Transcriptomes Waterfowl |
| title | Promotion of Th1 and Th2 responses over Th17 in Riemerella anatipestifer stimulation in chicken splenocytes: Correlation of gga-miR-456-3p and gga-miR-16-5p with NOS2 and CCL5 expression |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-10T16%3A49%3A14IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Promotion%20of%20Th1%20and%20Th2%20responses%20over%20Th17%20in%20Riemerella%20anatipestifer%20stimulation%20in%20chicken%20splenocytes:%20Correlation%20of%20gga-miR-456-3p%20and%20gga-miR-16-5p%20with%20NOS2%20and%20CCL5%20expression&rft.jtitle=PloS%20one&rft.au=Cammayo-Fletcher,%20Paula%20Leona%20T&rft.date=2023-11-06&rft.volume=18&rft.issue=11&rft.spage=e0294031&rft.epage=e0294031&rft.pages=e0294031-e0294031&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0294031&rft_dat=%3Cgale_plos_%3EA771800362%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=3069280550&rft_id=info:pmid/&rft_galeid=A771800362&rft_doaj_id=oai_doaj_org_article_c20ee8cc0cee486985523773179c162d&rfr_iscdi=true |