Assessment of temperature and time on the survivability of porcine reproductive and respiratory syndrome virus (PRRSV) and porcine epidemic diarrhea virus (PEDV) on experimentally contaminated surfaces
Fomites might be responsible for virus introduction in swine farms, highlighting the importance of implementing practices to minimize the probability of virus introduction. The study's objective was to assess the efficacy of different combinations of temperatures and holding-times on detecting...
Gespeichert in:
| Veröffentlicht in: | PloS one 2024-01, Vol.19 (1), p.e0291181-e0291181 |
|---|---|
| Hauptverfasser: | , , , , , , , , , , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | e0291181 |
|---|---|
| container_issue | 1 |
| container_start_page | e0291181 |
| container_title | PloS one |
| container_volume | 19 |
| creator | Mil-Homens, Mafalda Aljets, Ethan Paiva, Rodrigo C Machado, Isadora Cezar, Guilherme Osemeke, Onyekachukwu Moraes, Daniel Jayaraman, Swaminathan Brinning, Mckenna Poeta Silva, Ana Paula Tidgren, Lauren Durflinger, Madison Wilhelm, Mallory Flores, Vivian Frenier, Jolie Linhares, Daniel Zhang, Jianqiang Holtkamp, Derald Silva, Gustavo S |
| description | Fomites might be responsible for virus introduction in swine farms, highlighting the importance of implementing practices to minimize the probability of virus introduction. The study's objective was to assess the efficacy of different combinations of temperatures and holding-times on detecting live PRRSV and PEDV on surfaces commonly found in supply entry rooms in swine farms. Two PRRSV isolates (MN 184 and 1-4-4 L1C variant) and one PEDV isolate (NC 49469/2013) were inoculated on cardboard and aluminum. An experimental study tested combinations of four temperatures (20°C, 30°C, 40°C, and 50°C) and six holding-times (15 minutes, 60 minutes, 6 hours, 12 hours, 24 hours, and 36 hours) for the presence of the viruses on each surface type. After virus titration, virus presence was assessed by assessing the cytopathic effects and immunofluorescence staining. The titers were expressed as log10 TCID50/ml, and regression models; half-lives equations were calculated to assess differences between treatments and time to not detect the live virus. The results suggest that the minimum time that surfaces should be held to not detect the virus at 30°C was 24 hours, 40°C required 12 hours, and 50°C required 6 hours; aluminum surfaces took longer to reach the desired temperature compared to cardboard. The results suggest that PRRSV 1-4-4 L1C variant had higher half-lives at higher temperatures than PRRSV MN 184. In conclusion, time and temperature combinations effectively decrease the concentration of PRRSV and PEDV on different surfaces found in supply entry rooms in swine farms. |
| doi_str_mv | 10.1371/journal.pone.0291181 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_plos_</sourceid><recordid>TN_cdi_plos_journals_3069213997</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><doaj_id>oai_doaj_org_article_bd75b2cf44c14d5bac47336adbb87df5</doaj_id><sourcerecordid>2917553485</sourcerecordid><originalsourceid>FETCH-LOGICAL-c420t-ef6815aa9c954a2c72c6b71f352764cc16676a026fd16f062cf94737ac521043</originalsourceid><addsrcrecordid>eNptks1u1DAUhSMEoqXwBggsdVMWM_gvdrKsSoFKlUClYms59g31KImD7YzII_JWOJ0fIcTKV9Z3zj3SPUXxmuA1YZK83_gpDLpbj36ANaY1IRV5UpySmtGVoJg9_Ws-KV7EuMG4ZJUQz4sTVlFOKKlPi9-XMUKMPQwJ-RYl6EcIOk0BkB4sSq4H5AeUHgDFKWzdVjeuc2le4NEH4wZAAcbg7WSS2-5UAeLososPM4rzYIPPLlsXpoguvt7dffv-7hE76GF0FnpnkHU6hAfQR_b6Q0bzeviVU7klpO66GRmfh94NOoFdYrXaQHxZPGt1F-HV_j0r7j9e3199Xt1--XRzdXm7MpzitIJWVKTUujZ1yTU1khrRSNKykkrBjSFCSKExFa0losWCmrbmkkltSkowZ2fF253t2Pmo9keIimFRU8LqWmbiZkdYrzdqzLl1mJXXTj1--PBD6ZCc6UA1VpZN3sC5IdyWjTZ5FRPaNk0lbVtmr4v9tuB_ThCT6l000HV6AD9Flc8uy5LxakHP_0H_H47vKBN8jAHaY0CC1dKrg0otvVL7XmXZm7351PRgj6JDkdgfTGHPtA</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>3069213997</pqid></control><display><type>article</type><title>Assessment of temperature and time on the survivability of porcine reproductive and respiratory syndrome virus (PRRSV) and porcine epidemic diarrhea virus (PEDV) on experimentally contaminated surfaces</title><source>DOAJ Directory of Open Access Journals</source><source>Public Library of Science (PLoS)</source><source>EZB-FREE-00999 freely available EZB journals</source><source>PubMed Central</source><source>Free Full-Text Journals in Chemistry</source><creator>Mil-Homens, Mafalda ; Aljets, Ethan ; Paiva, Rodrigo C ; Machado, Isadora ; Cezar, Guilherme ; Osemeke, Onyekachukwu ; Moraes, Daniel ; Jayaraman, Swaminathan ; Brinning, Mckenna ; Poeta Silva, Ana Paula ; Tidgren, Lauren ; Durflinger, Madison ; Wilhelm, Mallory ; Flores, Vivian ; Frenier, Jolie ; Linhares, Daniel ; Zhang, Jianqiang ; Holtkamp, Derald ; Silva, Gustavo S</creator><contributor>Gladue, Douglas</contributor><creatorcontrib>Mil-Homens, Mafalda ; Aljets, Ethan ; Paiva, Rodrigo C ; Machado, Isadora ; Cezar, Guilherme ; Osemeke, Onyekachukwu ; Moraes, Daniel ; Jayaraman, Swaminathan ; Brinning, Mckenna ; Poeta Silva, Ana Paula ; Tidgren, Lauren ; Durflinger, Madison ; Wilhelm, Mallory ; Flores, Vivian ; Frenier, Jolie ; Linhares, Daniel ; Zhang, Jianqiang ; Holtkamp, Derald ; Silva, Gustavo S ; Gladue, Douglas</creatorcontrib><description>Fomites might be responsible for virus introduction in swine farms, highlighting the importance of implementing practices to minimize the probability of virus introduction. The study's objective was to assess the efficacy of different combinations of temperatures and holding-times on detecting live PRRSV and PEDV on surfaces commonly found in supply entry rooms in swine farms. Two PRRSV isolates (MN 184 and 1-4-4 L1C variant) and one PEDV isolate (NC 49469/2013) were inoculated on cardboard and aluminum. An experimental study tested combinations of four temperatures (20°C, 30°C, 40°C, and 50°C) and six holding-times (15 minutes, 60 minutes, 6 hours, 12 hours, 24 hours, and 36 hours) for the presence of the viruses on each surface type. After virus titration, virus presence was assessed by assessing the cytopathic effects and immunofluorescence staining. The titers were expressed as log10 TCID50/ml, and regression models; half-lives equations were calculated to assess differences between treatments and time to not detect the live virus. The results suggest that the minimum time that surfaces should be held to not detect the virus at 30°C was 24 hours, 40°C required 12 hours, and 50°C required 6 hours; aluminum surfaces took longer to reach the desired temperature compared to cardboard. The results suggest that PRRSV 1-4-4 L1C variant had higher half-lives at higher temperatures than PRRSV MN 184. In conclusion, time and temperature combinations effectively decrease the concentration of PRRSV and PEDV on different surfaces found in supply entry rooms in swine farms.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0291181</identifier><identifier>PMID: 38241219</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Aluminum ; Animal diseases ; Cardboard ; Diarrhea ; Discount coupons ; Economic impact ; Farms ; Fomites ; Half-life ; High temperature ; Immunofluorescence ; Monoclonal antibodies ; Regression analysis ; Regression models ; Statistical analysis ; Survivability ; Swine ; Temperature ; Titration ; Transmissible gastroenteritis ; Variance analysis ; Viral diseases ; Viruses</subject><ispartof>PloS one, 2024-01, Vol.19 (1), p.e0291181-e0291181</ispartof><rights>Copyright: © 2024 Mil-Homens et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.</rights><rights>2024 Mil-Homens et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2024 Mil-Homens et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c420t-ef6815aa9c954a2c72c6b71f352764cc16676a026fd16f062cf94737ac521043</cites><orcidid>0000-0002-6069-5014 ; 0000-0002-7581-3482 ; 0000-0003-3554-457X ; 0000-0001-5884-8803</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0291181&type=printable$$EPDF$$P50$$Gplos$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://journals.plos.org/plosone/article?id=10.1371/journal.pone.0291181$$EHTML$$P50$$Gplos$$Hfree_for_read</linktohtml><link.rule.ids>314,780,784,864,2100,2926,23865,27923,27924,79371,79372</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/38241219$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Gladue, Douglas</contributor><creatorcontrib>Mil-Homens, Mafalda</creatorcontrib><creatorcontrib>Aljets, Ethan</creatorcontrib><creatorcontrib>Paiva, Rodrigo C</creatorcontrib><creatorcontrib>Machado, Isadora</creatorcontrib><creatorcontrib>Cezar, Guilherme</creatorcontrib><creatorcontrib>Osemeke, Onyekachukwu</creatorcontrib><creatorcontrib>Moraes, Daniel</creatorcontrib><creatorcontrib>Jayaraman, Swaminathan</creatorcontrib><creatorcontrib>Brinning, Mckenna</creatorcontrib><creatorcontrib>Poeta Silva, Ana Paula</creatorcontrib><creatorcontrib>Tidgren, Lauren</creatorcontrib><creatorcontrib>Durflinger, Madison</creatorcontrib><creatorcontrib>Wilhelm, Mallory</creatorcontrib><creatorcontrib>Flores, Vivian</creatorcontrib><creatorcontrib>Frenier, Jolie</creatorcontrib><creatorcontrib>Linhares, Daniel</creatorcontrib><creatorcontrib>Zhang, Jianqiang</creatorcontrib><creatorcontrib>Holtkamp, Derald</creatorcontrib><creatorcontrib>Silva, Gustavo S</creatorcontrib><title>Assessment of temperature and time on the survivability of porcine reproductive and respiratory syndrome virus (PRRSV) and porcine epidemic diarrhea virus (PEDV) on experimentally contaminated surfaces</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Fomites might be responsible for virus introduction in swine farms, highlighting the importance of implementing practices to minimize the probability of virus introduction. The study's objective was to assess the efficacy of different combinations of temperatures and holding-times on detecting live PRRSV and PEDV on surfaces commonly found in supply entry rooms in swine farms. Two PRRSV isolates (MN 184 and 1-4-4 L1C variant) and one PEDV isolate (NC 49469/2013) were inoculated on cardboard and aluminum. An experimental study tested combinations of four temperatures (20°C, 30°C, 40°C, and 50°C) and six holding-times (15 minutes, 60 minutes, 6 hours, 12 hours, 24 hours, and 36 hours) for the presence of the viruses on each surface type. After virus titration, virus presence was assessed by assessing the cytopathic effects and immunofluorescence staining. The titers were expressed as log10 TCID50/ml, and regression models; half-lives equations were calculated to assess differences between treatments and time to not detect the live virus. The results suggest that the minimum time that surfaces should be held to not detect the virus at 30°C was 24 hours, 40°C required 12 hours, and 50°C required 6 hours; aluminum surfaces took longer to reach the desired temperature compared to cardboard. The results suggest that PRRSV 1-4-4 L1C variant had higher half-lives at higher temperatures than PRRSV MN 184. In conclusion, time and temperature combinations effectively decrease the concentration of PRRSV and PEDV on different surfaces found in supply entry rooms in swine farms.</description><subject>Aluminum</subject><subject>Animal diseases</subject><subject>Cardboard</subject><subject>Diarrhea</subject><subject>Discount coupons</subject><subject>Economic impact</subject><subject>Farms</subject><subject>Fomites</subject><subject>Half-life</subject><subject>High temperature</subject><subject>Immunofluorescence</subject><subject>Monoclonal antibodies</subject><subject>Regression analysis</subject><subject>Regression models</subject><subject>Statistical analysis</subject><subject>Survivability</subject><subject>Swine</subject><subject>Temperature</subject><subject>Titration</subject><subject>Transmissible gastroenteritis</subject><subject>Variance analysis</subject><subject>Viral diseases</subject><subject>Viruses</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>DOA</sourceid><recordid>eNptks1u1DAUhSMEoqXwBggsdVMWM_gvdrKsSoFKlUClYms59g31KImD7YzII_JWOJ0fIcTKV9Z3zj3SPUXxmuA1YZK83_gpDLpbj36ANaY1IRV5UpySmtGVoJg9_Ws-KV7EuMG4ZJUQz4sTVlFOKKlPi9-XMUKMPQwJ-RYl6EcIOk0BkB4sSq4H5AeUHgDFKWzdVjeuc2le4NEH4wZAAcbg7WSS2-5UAeLososPM4rzYIPPLlsXpoguvt7dffv-7hE76GF0FnpnkHU6hAfQR_b6Q0bzeviVU7klpO66GRmfh94NOoFdYrXaQHxZPGt1F-HV_j0r7j9e3199Xt1--XRzdXm7MpzitIJWVKTUujZ1yTU1khrRSNKykkrBjSFCSKExFa0losWCmrbmkkltSkowZ2fF253t2Pmo9keIimFRU8LqWmbiZkdYrzdqzLl1mJXXTj1--PBD6ZCc6UA1VpZN3sC5IdyWjTZ5FRPaNk0lbVtmr4v9tuB_ThCT6l000HV6AD9Flc8uy5LxakHP_0H_H47vKBN8jAHaY0CC1dKrg0otvVL7XmXZm7351PRgj6JDkdgfTGHPtA</recordid><startdate>20240101</startdate><enddate>20240101</enddate><creator>Mil-Homens, Mafalda</creator><creator>Aljets, Ethan</creator><creator>Paiva, Rodrigo C</creator><creator>Machado, Isadora</creator><creator>Cezar, Guilherme</creator><creator>Osemeke, Onyekachukwu</creator><creator>Moraes, Daniel</creator><creator>Jayaraman, Swaminathan</creator><creator>Brinning, Mckenna</creator><creator>Poeta Silva, Ana Paula</creator><creator>Tidgren, Lauren</creator><creator>Durflinger, Madison</creator><creator>Wilhelm, Mallory</creator><creator>Flores, Vivian</creator><creator>Frenier, Jolie</creator><creator>Linhares, Daniel</creator><creator>Zhang, Jianqiang</creator><creator>Holtkamp, Derald</creator><creator>Silva, Gustavo S</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0002-6069-5014</orcidid><orcidid>https://orcid.org/0000-0002-7581-3482</orcidid><orcidid>https://orcid.org/0000-0003-3554-457X</orcidid><orcidid>https://orcid.org/0000-0001-5884-8803</orcidid></search><sort><creationdate>20240101</creationdate><title>Assessment of temperature and time on the survivability of porcine reproductive and respiratory syndrome virus (PRRSV) and porcine epidemic diarrhea virus (PEDV) on experimentally contaminated surfaces</title><author>Mil-Homens, Mafalda ; Aljets, Ethan ; Paiva, Rodrigo C ; Machado, Isadora ; Cezar, Guilherme ; Osemeke, Onyekachukwu ; Moraes, Daniel ; Jayaraman, Swaminathan ; Brinning, Mckenna ; Poeta Silva, Ana Paula ; Tidgren, Lauren ; Durflinger, Madison ; Wilhelm, Mallory ; Flores, Vivian ; Frenier, Jolie ; Linhares, Daniel ; Zhang, Jianqiang ; Holtkamp, Derald ; Silva, Gustavo S</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c420t-ef6815aa9c954a2c72c6b71f352764cc16676a026fd16f062cf94737ac521043</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Aluminum</topic><topic>Animal diseases</topic><topic>Cardboard</topic><topic>Diarrhea</topic><topic>Discount coupons</topic><topic>Economic impact</topic><topic>Farms</topic><topic>Fomites</topic><topic>Half-life</topic><topic>High temperature</topic><topic>Immunofluorescence</topic><topic>Monoclonal antibodies</topic><topic>Regression analysis</topic><topic>Regression models</topic><topic>Statistical analysis</topic><topic>Survivability</topic><topic>Swine</topic><topic>Temperature</topic><topic>Titration</topic><topic>Transmissible gastroenteritis</topic><topic>Variance analysis</topic><topic>Viral diseases</topic><topic>Viruses</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mil-Homens, Mafalda</creatorcontrib><creatorcontrib>Aljets, Ethan</creatorcontrib><creatorcontrib>Paiva, Rodrigo C</creatorcontrib><creatorcontrib>Machado, Isadora</creatorcontrib><creatorcontrib>Cezar, Guilherme</creatorcontrib><creatorcontrib>Osemeke, Onyekachukwu</creatorcontrib><creatorcontrib>Moraes, Daniel</creatorcontrib><creatorcontrib>Jayaraman, Swaminathan</creatorcontrib><creatorcontrib>Brinning, Mckenna</creatorcontrib><creatorcontrib>Poeta Silva, Ana Paula</creatorcontrib><creatorcontrib>Tidgren, Lauren</creatorcontrib><creatorcontrib>Durflinger, Madison</creatorcontrib><creatorcontrib>Wilhelm, Mallory</creatorcontrib><creatorcontrib>Flores, Vivian</creatorcontrib><creatorcontrib>Frenier, Jolie</creatorcontrib><creatorcontrib>Linhares, Daniel</creatorcontrib><creatorcontrib>Zhang, Jianqiang</creatorcontrib><creatorcontrib>Holtkamp, Derald</creatorcontrib><creatorcontrib>Silva, Gustavo S</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Nursing & Allied Health Database</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Meteorological & Geoastrophysical Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Meteorological & Geoastrophysical Abstracts - Academic</collection><collection>ProQuest Engineering Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Engineering Database</collection><collection>Nursing & Allied Health Premium</collection><collection>Advanced Technologies & Aerospace Database</collection><collection>ProQuest Advanced Technologies & Aerospace Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>Materials Science Collection</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Engineering Collection</collection><collection>Environmental Science Collection</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mil-Homens, Mafalda</au><au>Aljets, Ethan</au><au>Paiva, Rodrigo C</au><au>Machado, Isadora</au><au>Cezar, Guilherme</au><au>Osemeke, Onyekachukwu</au><au>Moraes, Daniel</au><au>Jayaraman, Swaminathan</au><au>Brinning, Mckenna</au><au>Poeta Silva, Ana Paula</au><au>Tidgren, Lauren</au><au>Durflinger, Madison</au><au>Wilhelm, Mallory</au><au>Flores, Vivian</au><au>Frenier, Jolie</au><au>Linhares, Daniel</au><au>Zhang, Jianqiang</au><au>Holtkamp, Derald</au><au>Silva, Gustavo S</au><au>Gladue, Douglas</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Assessment of temperature and time on the survivability of porcine reproductive and respiratory syndrome virus (PRRSV) and porcine epidemic diarrhea virus (PEDV) on experimentally contaminated surfaces</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2024-01-01</date><risdate>2024</risdate><volume>19</volume><issue>1</issue><spage>e0291181</spage><epage>e0291181</epage><pages>e0291181-e0291181</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Fomites might be responsible for virus introduction in swine farms, highlighting the importance of implementing practices to minimize the probability of virus introduction. The study's objective was to assess the efficacy of different combinations of temperatures and holding-times on detecting live PRRSV and PEDV on surfaces commonly found in supply entry rooms in swine farms. Two PRRSV isolates (MN 184 and 1-4-4 L1C variant) and one PEDV isolate (NC 49469/2013) were inoculated on cardboard and aluminum. An experimental study tested combinations of four temperatures (20°C, 30°C, 40°C, and 50°C) and six holding-times (15 minutes, 60 minutes, 6 hours, 12 hours, 24 hours, and 36 hours) for the presence of the viruses on each surface type. After virus titration, virus presence was assessed by assessing the cytopathic effects and immunofluorescence staining. The titers were expressed as log10 TCID50/ml, and regression models; half-lives equations were calculated to assess differences between treatments and time to not detect the live virus. The results suggest that the minimum time that surfaces should be held to not detect the virus at 30°C was 24 hours, 40°C required 12 hours, and 50°C required 6 hours; aluminum surfaces took longer to reach the desired temperature compared to cardboard. The results suggest that PRRSV 1-4-4 L1C variant had higher half-lives at higher temperatures than PRRSV MN 184. In conclusion, time and temperature combinations effectively decrease the concentration of PRRSV and PEDV on different surfaces found in supply entry rooms in swine farms.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>38241219</pmid><doi>10.1371/journal.pone.0291181</doi><orcidid>https://orcid.org/0000-0002-6069-5014</orcidid><orcidid>https://orcid.org/0000-0002-7581-3482</orcidid><orcidid>https://orcid.org/0000-0003-3554-457X</orcidid><orcidid>https://orcid.org/0000-0001-5884-8803</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2024-01, Vol.19 (1), p.e0291181-e0291181 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_3069213997 |
| source | DOAJ Directory of Open Access Journals; Public Library of Science (PLoS); EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Aluminum Animal diseases Cardboard Diarrhea Discount coupons Economic impact Farms Fomites Half-life High temperature Immunofluorescence Monoclonal antibodies Regression analysis Regression models Statistical analysis Survivability Swine Temperature Titration Transmissible gastroenteritis Variance analysis Viral diseases Viruses |
| title | Assessment of temperature and time on the survivability of porcine reproductive and respiratory syndrome virus (PRRSV) and porcine epidemic diarrhea virus (PEDV) on experimentally contaminated surfaces |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-11T16%3A30%3A06IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Assessment%20of%20temperature%20and%20time%20on%20the%20survivability%20of%20porcine%20reproductive%20and%20respiratory%20syndrome%20virus%20(PRRSV)%20and%20porcine%20epidemic%20diarrhea%20virus%20(PEDV)%20on%20experimentally%20contaminated%20surfaces&rft.jtitle=PloS%20one&rft.au=Mil-Homens,%20Mafalda&rft.date=2024-01-01&rft.volume=19&rft.issue=1&rft.spage=e0291181&rft.epage=e0291181&rft.pages=e0291181-e0291181&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0291181&rft_dat=%3Cproquest_plos_%3E2917553485%3C/proquest_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=3069213997&rft_id=info:pmid/38241219&rft_doaj_id=oai_doaj_org_article_bd75b2cf44c14d5bac47336adbb87df5&rfr_iscdi=true |