Adipose tissue-derived exosomes alleviate particulate matter-induced inflammatory response and skin barrier damage in atopic dermatitis-like triple-cell model
Recently, particulate matter (PM) has been shown to exacerbate atopic dermatitis (AD) by inducing an inflammatory response. Meanwhile, several studies revealed that exosomes derived from adipose tissue-derived mesenchymal stem cells promote wound healing and alleviate inflammation via their regenera...
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| creator | Roh, Yoon Jin Choi, Yong Hee Shin, Sun Hye Lee, Mi-Kyung Won, Yu Jin Lee, Jun Ho Cho, Byong Seung Park, Kui Young Seo, Seong Jun |
| description | Recently, particulate matter (PM) has been shown to exacerbate atopic dermatitis (AD) by inducing an inflammatory response. Meanwhile, several studies revealed that exosomes derived from adipose tissue-derived mesenchymal stem cells promote wound healing and alleviate inflammation via their regenerative and immunomodulatory capacities. Our study aimed to investigate the effects of human adipose tissue-derived mesenchymal stem cell-derived (ASC)-exosomes in PM-induced AD. An AD-like triple-cell model was established by treating human keratinocytes, dermal fibroblasts, and mast cells with polyinosinic:polycytidylic acid (Poly I:C) and interleukin 1 alpha (IL-1α). The effects of PM and ASC-exosomes on the expression of pro-inflammatory cytokines and skin barrier proteins were examined using quantitative real-time polymerase chain reaction, western blotting, and immunofluorescence. PM increased pro-inflammatory cytokines (IL-6, IL-1β, and IL-1α) and decreased the anti-inflammatory cytokine IL-10, while the mRNA expression of skin barrier proteins (loricrin and filaggrin) decreased. However, when the cells were treated with ASC-exosomes, the PM-induced effects on pro-inflammatory cytokines and skin barrier proteins were reversed. Our results confirmed that PM-induced inflammation and skin barrier damage were alleviated by ASC-exosomes in our AD-like triple-cell model. These data suggest that ASC-exosomes can serve as a therapeutic agent for PM-exacerbated AD. |
| doi_str_mv | 10.1371/journal.pone.0292050 |
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Meanwhile, several studies revealed that exosomes derived from adipose tissue-derived mesenchymal stem cells promote wound healing and alleviate inflammation via their regenerative and immunomodulatory capacities. Our study aimed to investigate the effects of human adipose tissue-derived mesenchymal stem cell-derived (ASC)-exosomes in PM-induced AD. An AD-like triple-cell model was established by treating human keratinocytes, dermal fibroblasts, and mast cells with polyinosinic:polycytidylic acid (Poly I:C) and interleukin 1 alpha (IL-1α). The effects of PM and ASC-exosomes on the expression of pro-inflammatory cytokines and skin barrier proteins were examined using quantitative real-time polymerase chain reaction, western blotting, and immunofluorescence. PM increased pro-inflammatory cytokines (IL-6, IL-1β, and IL-1α) and decreased the anti-inflammatory cytokine IL-10, while the mRNA expression of skin barrier proteins (loricrin and filaggrin) decreased. However, when the cells were treated with ASC-exosomes, the PM-induced effects on pro-inflammatory cytokines and skin barrier proteins were reversed. Our results confirmed that PM-induced inflammation and skin barrier damage were alleviated by ASC-exosomes in our AD-like triple-cell model. These data suggest that ASC-exosomes can serve as a therapeutic agent for PM-exacerbated AD.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0292050</identifier><identifier>PMID: 38241278</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Adipose tissue ; Adipose tissues ; Advertising executives ; Air pollution ; Analysis ; Antibodies ; Atopic dermatitis ; Body fat ; Care and treatment ; Cell culture ; Composition ; Cytokines ; Damage ; Dermatitis ; Diagnosis ; Ethylenediaminetetraacetic acid ; Exosomes ; Filaggrin ; Gene expression ; Health aspects ; Immunofluorescence ; Immunomodulation ; Inflammation ; Inflammatory response ; Interleukin 1 ; Interleukins ; Keratinocytes ; Mast cells ; Measurement ; Mesenchymal stem cells ; Outdoor air quality ; Particles ; Particulate emissions ; Particulate matter ; Pharmacology ; Polyinosinic:polycytidylic acid ; Polymerase chain reaction ; Proteins ; Real time ; RNA ; Skin ; Stem cells ; Western blotting ; Wound healing</subject><ispartof>PloS one, 2024-01, Vol.19 (1), p.e0292050-e0292050</ispartof><rights>Copyright: © 2024 Roh et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.</rights><rights>COPYRIGHT 2024 Public Library of Science</rights><rights>2024 Roh et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2024 Roh et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. 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These data suggest that ASC-exosomes can serve as a therapeutic agent for PM-exacerbated AD.</description><subject>Adipose tissue</subject><subject>Adipose tissues</subject><subject>Advertising executives</subject><subject>Air pollution</subject><subject>Analysis</subject><subject>Antibodies</subject><subject>Atopic dermatitis</subject><subject>Body fat</subject><subject>Care and treatment</subject><subject>Cell culture</subject><subject>Composition</subject><subject>Cytokines</subject><subject>Damage</subject><subject>Dermatitis</subject><subject>Diagnosis</subject><subject>Ethylenediaminetetraacetic acid</subject><subject>Exosomes</subject><subject>Filaggrin</subject><subject>Gene expression</subject><subject>Health aspects</subject><subject>Immunofluorescence</subject><subject>Immunomodulation</subject><subject>Inflammation</subject><subject>Inflammatory response</subject><subject>Interleukin 1</subject><subject>Interleukins</subject><subject>Keratinocytes</subject><subject>Mast 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tissue-derived exosomes alleviate particulate matter-induced inflammatory response and skin barrier damage in atopic dermatitis-like triple-cell model</title><author>Roh, Yoon Jin ; Choi, Yong Hee ; Shin, Sun Hye ; Lee, Mi-Kyung ; Won, Yu Jin ; Lee, Jun Ho ; Cho, Byong Seung ; Park, Kui Young ; Seo, Seong Jun</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c637t-211a3a579eb588768f073f01c75fac7f42a40186d4c72746f8f76f098838034d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Adipose tissue</topic><topic>Adipose tissues</topic><topic>Advertising executives</topic><topic>Air pollution</topic><topic>Analysis</topic><topic>Antibodies</topic><topic>Atopic dermatitis</topic><topic>Body fat</topic><topic>Care and treatment</topic><topic>Cell 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Meanwhile, several studies revealed that exosomes derived from adipose tissue-derived mesenchymal stem cells promote wound healing and alleviate inflammation via their regenerative and immunomodulatory capacities. Our study aimed to investigate the effects of human adipose tissue-derived mesenchymal stem cell-derived (ASC)-exosomes in PM-induced AD. An AD-like triple-cell model was established by treating human keratinocytes, dermal fibroblasts, and mast cells with polyinosinic:polycytidylic acid (Poly I:C) and interleukin 1 alpha (IL-1α). The effects of PM and ASC-exosomes on the expression of pro-inflammatory cytokines and skin barrier proteins were examined using quantitative real-time polymerase chain reaction, western blotting, and immunofluorescence. PM increased pro-inflammatory cytokines (IL-6, IL-1β, and IL-1α) and decreased the anti-inflammatory cytokine IL-10, while the mRNA expression of skin barrier proteins (loricrin and filaggrin) decreased. However, when the cells were treated with ASC-exosomes, the PM-induced effects on pro-inflammatory cytokines and skin barrier proteins were reversed. Our results confirmed that PM-induced inflammation and skin barrier damage were alleviated by ASC-exosomes in our AD-like triple-cell model. These data suggest that ASC-exosomes can serve as a therapeutic agent for PM-exacerbated AD.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>38241278</pmid><doi>10.1371/journal.pone.0292050</doi><tpages>e0292050</tpages><orcidid>https://orcid.org/0000-0002-3201-6408</orcidid><orcidid>https://orcid.org/0000-0001-5965-1754</orcidid><oa>free_for_read</oa></addata></record> |
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| ispartof | PloS one, 2024-01, Vol.19 (1), p.e0292050-e0292050 |
| issn | 1932-6203 1932-6203 |
| language | eng |
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| source | DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; Free Full-Text Journals in Chemistry; Public Library of Science (PLoS) |
| subjects | Adipose tissue Adipose tissues Advertising executives Air pollution Analysis Antibodies Atopic dermatitis Body fat Care and treatment Cell culture Composition Cytokines Damage Dermatitis Diagnosis Ethylenediaminetetraacetic acid Exosomes Filaggrin Gene expression Health aspects Immunofluorescence Immunomodulation Inflammation Inflammatory response Interleukin 1 Interleukins Keratinocytes Mast cells Measurement Mesenchymal stem cells Outdoor air quality Particles Particulate emissions Particulate matter Pharmacology Polyinosinic:polycytidylic acid Polymerase chain reaction Proteins Real time RNA Skin Stem cells Western blotting Wound healing |
| title | Adipose tissue-derived exosomes alleviate particulate matter-induced inflammatory response and skin barrier damage in atopic dermatitis-like triple-cell model |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-13T04%3A23%3A37IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Adipose%20tissue-derived%20exosomes%20alleviate%20particulate%20matter-induced%20inflammatory%20response%20and%20skin%20barrier%20damage%20in%20atopic%20dermatitis-like%20triple-cell%20model&rft.jtitle=PloS%20one&rft.au=Roh,%20Yoon%20Jin&rft.date=2024-01-19&rft.volume=19&rft.issue=1&rft.spage=e0292050&rft.epage=e0292050&rft.pages=e0292050-e0292050&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0292050&rft_dat=%3Cgale_plos_%3EA779822026%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=3069213983&rft_id=info:pmid/38241278&rft_galeid=A779822026&rft_doaj_id=oai_doaj_org_article_98256eb82dd449249b0b98a59bcaace3&rfr_iscdi=true |