Methods optimization for the expression and purification of human calcium calmodulin-dependent protein kinase II alpha

Calcium/calmodulin-dependent protein kinase II (CaMKII) is a complex multifunctional kinase that is highly expressed in central nervous tissues and plays a key regulatory role in the calcium signaling pathway. Despite over 30 years of recombinant expression and characterization studies, CaMKII conti...

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Veröffentlicht in:	PloS one 2024-01, Vol.19 (1), p.e0285651-e0285651
Hauptverfasser:	Bolton, Scott C, Thompson, David H, Kinzer-Ursem, Tamara L
Format:	Artikel
Sprache:	eng
Schlagworte:	Analysis Baculoviridae - genetics Baculovirus Biophysics Ca2+/calmodulin-dependent protein kinase II Calcium Calcium compounds Calcium Signaling Calcium signalling Calcium-Calmodulin-Dependent Protein Kinase Type 2 - genetics Calmodulin Chromatography E coli Enzymes Escherichia coli Escherichia coli - genetics Ethylenediaminetetraacetic acid Humans Insects Kinases Methods Nervous tissues Phosphatase Protein kinases Protein purification Proteins Purification Signal transduction Substrates
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