Photobiomodulation mitigates Bothrops jararacussu venom-induced damage in myoblast cells by enhancing myogenic factors and reducing cytokine production
Photobiomodulation has exhibited promise in mitigating the local effects induced by Bothrops snakebite envenoming; however, the mechanisms underlying this protection are not yet fully understood. Herein, the effectiveness of photobiomodulation effects on regenerative response of C2C12 myoblast cells...
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| Veröffentlicht in: | PLoS neglected tropical diseases 2024-05, Vol.18 (5), p.e0012227 |
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| creator | Silva, Luciana Miato Gonçalves Gouveia, Viviane Almeida Campos, Gabriela Russo Soeiro Dale, Camila Squarzone da Palma, Renata Kelly de Oliveira, Ana Paula Ligeiro Marcos, Rodrigo Labat Duran, Cinthya Cosme Gutierrez Cogo, José Carlos Silva Junior, José Antônio Zamuner, Stella Regina |
| description | Photobiomodulation has exhibited promise in mitigating the local effects induced by Bothrops snakebite envenoming; however, the mechanisms underlying this protection are not yet fully understood. Herein, the effectiveness of photobiomodulation effects on regenerative response of C2C12 myoblast cells following exposure to Bothrops jararacussu venom (BjsuV), as well as the mechanisms involved was investigated.
C2C12 myoblast cells were exposed to BjsuV (12.5 μg/mL) and irradiated once for 10 seconds with laser light of 660 nm (14.08 mW; 0.04 cm2; 352 mW/cm2) or 780 nm (17.6 mW; 0.04 cm2; 440 mW/ cm2) to provide energy densities of 3.52 and 4.4 J/cm2, and total energies of 0.1408 and 0.176 J, respectively. Cell migration was assessed through a wound-healing assay. The expression of MAPK p38-α, NF-Кβ, Myf5, Pax-7, MyoD, and myogenin proteins were assessed by western blotting analysis. In addition, interleukin IL1-β, IL-6, TNF-alfa and IL-10 levels were measured in the supernatant by ELISA. The PBM applied to C2C12 cells exposed to BjsuV promoted cell migration, increase the expression of myogenic factors (Pax7, MyF5, MyoD and myogenin), reduced the levels of proinflammatory cytokines, IL1-β, IL-6, TNF-alfa, and increased the levels of anti-inflammatory cytokine IL-10. In addition, PBM downregulates the expression of NF-kB, and had no effect on p38 MAKP.
These data demonstrated that protection of the muscle cell by PBM seems to be related to the increase of myogenic factors as well as the modulation of inflammatory mediators. PBM therapy may offer a new therapeutic strategy to address the local effects of snakebite envenoming by promoting muscle regeneration and reducing the inflammatory process. |
| doi_str_mv | 10.1371/journal.pntd.0012227 |
| format | Article |
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C2C12 myoblast cells were exposed to BjsuV (12.5 μg/mL) and irradiated once for 10 seconds with laser light of 660 nm (14.08 mW; 0.04 cm2; 352 mW/cm2) or 780 nm (17.6 mW; 0.04 cm2; 440 mW/ cm2) to provide energy densities of 3.52 and 4.4 J/cm2, and total energies of 0.1408 and 0.176 J, respectively. Cell migration was assessed through a wound-healing assay. The expression of MAPK p38-α, NF-Кβ, Myf5, Pax-7, MyoD, and myogenin proteins were assessed by western blotting analysis. In addition, interleukin IL1-β, IL-6, TNF-alfa and IL-10 levels were measured in the supernatant by ELISA. The PBM applied to C2C12 cells exposed to BjsuV promoted cell migration, increase the expression of myogenic factors (Pax7, MyF5, MyoD and myogenin), reduced the levels of proinflammatory cytokines, IL1-β, IL-6, TNF-alfa, and increased the levels of anti-inflammatory cytokine IL-10. In addition, PBM downregulates the expression of NF-kB, and had no effect on p38 MAKP.
These data demonstrated that protection of the muscle cell by PBM seems to be related to the increase of myogenic factors as well as the modulation of inflammatory mediators. PBM therapy may offer a new therapeutic strategy to address the local effects of snakebite envenoming by promoting muscle regeneration and reducing the inflammatory process.</description><identifier>ISSN: 1935-2735</identifier><identifier>ISSN: 1935-2727</identifier><identifier>EISSN: 1935-2735</identifier><identifier>DOI: 10.1371/journal.pntd.0012227</identifier><identifier>PMID: 38814992</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Aluminum ; Analysis ; Biology and Life Sciences ; Bothrops jararacussu ; Cell adhesion & migration ; Cell migration ; Cells ; Cytokines ; ELISA ; Engineering and Technology ; Enzyme-linked immunosorbent assay ; Experiments ; Exposure ; Inflammation ; Interleukin 10 ; Interleukin 6 ; Interleukins ; Lasers ; MAP kinase ; Medicine and Health Sciences ; Methods ; Muscles ; Musculoskeletal system ; Myoblasts ; MyoD protein ; Myogenin ; NF-κB protein ; Phototherapy ; Physiological aspects ; Production management ; Proteins ; Regeneration (biological) ; Snake bites ; Venom ; Western blotting ; Wound healing</subject><ispartof>PLoS neglected tropical diseases, 2024-05, Vol.18 (5), p.e0012227</ispartof><rights>Copyright: © 2024 Silva et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.</rights><rights>COPYRIGHT 2024 Public Library of Science</rights><rights>2024 Silva et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2024 Silva et al 2024 Silva et al</rights><rights>2024 Silva et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c504t-d2d1cc544d9eb6dd5b16811ad2fb742f988731e44b6846bf1ee6b2117f52ba1c3</cites><orcidid>0000-0002-4694-7617</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC11192417/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC11192417/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,2095,2914,23846,27903,27904,53770,53772,79347,79348</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/38814992$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Santoro, Marcelo Larami</contributor><creatorcontrib>Silva, Luciana Miato Gonçalves</creatorcontrib><creatorcontrib>Gouveia, Viviane Almeida</creatorcontrib><creatorcontrib>Campos, Gabriela Russo Soeiro</creatorcontrib><creatorcontrib>Dale, Camila Squarzone</creatorcontrib><creatorcontrib>da Palma, Renata Kelly</creatorcontrib><creatorcontrib>de Oliveira, Ana Paula Ligeiro</creatorcontrib><creatorcontrib>Marcos, Rodrigo Labat</creatorcontrib><creatorcontrib>Duran, Cinthya Cosme Gutierrez</creatorcontrib><creatorcontrib>Cogo, José Carlos</creatorcontrib><creatorcontrib>Silva Junior, José Antônio</creatorcontrib><creatorcontrib>Zamuner, Stella Regina</creatorcontrib><title>Photobiomodulation mitigates Bothrops jararacussu venom-induced damage in myoblast cells by enhancing myogenic factors and reducing cytokine production</title><title>PLoS neglected tropical diseases</title><addtitle>PLoS Negl Trop Dis</addtitle><description>Photobiomodulation has exhibited promise in mitigating the local effects induced by Bothrops snakebite envenoming; however, the mechanisms underlying this protection are not yet fully understood. Herein, the effectiveness of photobiomodulation effects on regenerative response of C2C12 myoblast cells following exposure to Bothrops jararacussu venom (BjsuV), as well as the mechanisms involved was investigated.
C2C12 myoblast cells were exposed to BjsuV (12.5 μg/mL) and irradiated once for 10 seconds with laser light of 660 nm (14.08 mW; 0.04 cm2; 352 mW/cm2) or 780 nm (17.6 mW; 0.04 cm2; 440 mW/ cm2) to provide energy densities of 3.52 and 4.4 J/cm2, and total energies of 0.1408 and 0.176 J, respectively. Cell migration was assessed through a wound-healing assay. The expression of MAPK p38-α, NF-Кβ, Myf5, Pax-7, MyoD, and myogenin proteins were assessed by western blotting analysis. In addition, interleukin IL1-β, IL-6, TNF-alfa and IL-10 levels were measured in the supernatant by ELISA. The PBM applied to C2C12 cells exposed to BjsuV promoted cell migration, increase the expression of myogenic factors (Pax7, MyF5, MyoD and myogenin), reduced the levels of proinflammatory cytokines, IL1-β, IL-6, TNF-alfa, and increased the levels of anti-inflammatory cytokine IL-10. In addition, PBM downregulates the expression of NF-kB, and had no effect on p38 MAKP.
These data demonstrated that protection of the muscle cell by PBM seems to be related to the increase of myogenic factors as well as the modulation of inflammatory mediators. PBM therapy may offer a new therapeutic strategy to address the local effects of snakebite envenoming by promoting muscle regeneration and reducing the inflammatory process.</description><subject>Aluminum</subject><subject>Analysis</subject><subject>Biology and Life Sciences</subject><subject>Bothrops jararacussu</subject><subject>Cell adhesion & migration</subject><subject>Cell migration</subject><subject>Cells</subject><subject>Cytokines</subject><subject>ELISA</subject><subject>Engineering and Technology</subject><subject>Enzyme-linked immunosorbent assay</subject><subject>Experiments</subject><subject>Exposure</subject><subject>Inflammation</subject><subject>Interleukin 10</subject><subject>Interleukin 6</subject><subject>Interleukins</subject><subject>Lasers</subject><subject>MAP kinase</subject><subject>Medicine and Health Sciences</subject><subject>Methods</subject><subject>Muscles</subject><subject>Musculoskeletal system</subject><subject>Myoblasts</subject><subject>MyoD protein</subject><subject>Myogenin</subject><subject>NF-κB protein</subject><subject>Phototherapy</subject><subject>Physiological aspects</subject><subject>Production management</subject><subject>Proteins</subject><subject>Regeneration (biological)</subject><subject>Snake bites</subject><subject>Venom</subject><subject>Western blotting</subject><subject>Wound 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Larami</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Photobiomodulation mitigates Bothrops jararacussu venom-induced damage in myoblast cells by enhancing myogenic factors and reducing cytokine production</atitle><jtitle>PLoS neglected tropical diseases</jtitle><addtitle>PLoS Negl Trop Dis</addtitle><date>2024-05-30</date><risdate>2024</risdate><volume>18</volume><issue>5</issue><spage>e0012227</spage><pages>e0012227-</pages><issn>1935-2735</issn><issn>1935-2727</issn><eissn>1935-2735</eissn><abstract>Photobiomodulation has exhibited promise in mitigating the local effects induced by Bothrops snakebite envenoming; however, the mechanisms underlying this protection are not yet fully understood. Herein, the effectiveness of photobiomodulation effects on regenerative response of C2C12 myoblast cells following exposure to Bothrops jararacussu venom (BjsuV), as well as the mechanisms involved was investigated.
C2C12 myoblast cells were exposed to BjsuV (12.5 μg/mL) and irradiated once for 10 seconds with laser light of 660 nm (14.08 mW; 0.04 cm2; 352 mW/cm2) or 780 nm (17.6 mW; 0.04 cm2; 440 mW/ cm2) to provide energy densities of 3.52 and 4.4 J/cm2, and total energies of 0.1408 and 0.176 J, respectively. Cell migration was assessed through a wound-healing assay. The expression of MAPK p38-α, NF-Кβ, Myf5, Pax-7, MyoD, and myogenin proteins were assessed by western blotting analysis. In addition, interleukin IL1-β, IL-6, TNF-alfa and IL-10 levels were measured in the supernatant by ELISA. The PBM applied to C2C12 cells exposed to BjsuV promoted cell migration, increase the expression of myogenic factors (Pax7, MyF5, MyoD and myogenin), reduced the levels of proinflammatory cytokines, IL1-β, IL-6, TNF-alfa, and increased the levels of anti-inflammatory cytokine IL-10. In addition, PBM downregulates the expression of NF-kB, and had no effect on p38 MAKP.
These data demonstrated that protection of the muscle cell by PBM seems to be related to the increase of myogenic factors as well as the modulation of inflammatory mediators. PBM therapy may offer a new therapeutic strategy to address the local effects of snakebite envenoming by promoting muscle regeneration and reducing the inflammatory process.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>38814992</pmid><doi>10.1371/journal.pntd.0012227</doi><orcidid>https://orcid.org/0000-0002-4694-7617</orcidid><oa>free_for_read</oa></addata></record> |
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| ispartof | PLoS neglected tropical diseases, 2024-05, Vol.18 (5), p.e0012227 |
| issn | 1935-2735 1935-2727 1935-2735 |
| language | eng |
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| source | PubMed Central Open Access; Public Library of Science (PLoS); DOAJ Directory of Open Access Journals; EZB-FREE-00999 freely available EZB journals; PubMed Central |
| subjects | Aluminum Analysis Biology and Life Sciences Bothrops jararacussu Cell adhesion & migration Cell migration Cells Cytokines ELISA Engineering and Technology Enzyme-linked immunosorbent assay Experiments Exposure Inflammation Interleukin 10 Interleukin 6 Interleukins Lasers MAP kinase Medicine and Health Sciences Methods Muscles Musculoskeletal system Myoblasts MyoD protein Myogenin NF-κB protein Phototherapy Physiological aspects Production management Proteins Regeneration (biological) Snake bites Venom Western blotting Wound healing |
| title | Photobiomodulation mitigates Bothrops jararacussu venom-induced damage in myoblast cells by enhancing myogenic factors and reducing cytokine production |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-22T13%3A56%3A54IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Photobiomodulation%20mitigates%20Bothrops%20jararacussu%20venom-induced%20damage%20in%20myoblast%20cells%20by%20enhancing%20myogenic%20factors%20and%20reducing%20cytokine%20production&rft.jtitle=PLoS%20neglected%20tropical%20diseases&rft.au=Silva,%20Luciana%20Miato%20Gon%C3%A7alves&rft.date=2024-05-30&rft.volume=18&rft.issue=5&rft.spage=e0012227&rft.pages=e0012227-&rft.issn=1935-2735&rft.eissn=1935-2735&rft_id=info:doi/10.1371/journal.pntd.0012227&rft_dat=%3Cgale_plos_%3EA796130719%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=3069186316&rft_id=info:pmid/38814992&rft_galeid=A796130719&rft_doaj_id=oai_doaj_org_article_f64fc8f510ab4132a89c8f8f35cf3fe8&rfr_iscdi=true |