Cryptic circulation of chikungunya virus in São Jose do Rio Preto, Brazil, 2015-2019
Chikungunya virus (CHIKV) has spread across Brazil with varying incidence rates depending on the affected areas. Due to cocirculation of arboviruses and overlapping disease symptoms, CHIKV infection may be underdiagnosed. To understand the lack of CHIKV epidemics in São José do Rio Preto (SJdRP), Sã...
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| creator | Zini, Nathalia Ávila, Matheus Henrique Tavares Cezarotti, Natalia Morbi Parra, Maisa Carla Pereira Banho, Cecília Artico Sacchetto, Livia Negri, Andreia Francesli Araújo, Emerson Bittar, Cintia Milhin, Bruno Henrique Gonçalves de Aguiar Miranda Hernandes, Victor Dutra, Karina Rocha Trigo, Leonardo Agopian Cecílio da Rocha, Leonardo Alves da Silva, Rafael Celestino Dutra da Silva, Gislaine Fernanda Pereira Dos Santos, Tamires de Carvalho Marques, Beatriz Lopes Dos Santos, Andresa Augusto, Marcos Tayar Mistrão, Natalia Franco Bueno Ribeiro, Milene Rocha Pinheiro, Tauyne Menegaldo Maria Izabel Lopes Dos Santos, Thayza Avilla, Clarita Maria Secco Bernardi, Victoria Freitas, Caroline Gandolfi, Flora de Andrade Ferraz Júnior, Hélio Correa Perim, Gabriela Camilotti Gomes, Mirella Cezare Garcia, Pedro Henrique Carrilho Rocha, Rodrigo Sborghi Galvão, Tayna Manfrin Fávaro, Eliane Aparecida Scamardi, Samuel Noah Rogovski, Karen Sanmartin Peixoto, Renan Luiz Benfatti, Luiza Cruz, Leonardo Teixeira Chama, Paula Patricia de Freitas Oliveira, Mânlio Tasso Watanabe, Aripuanã Sakurada Aranha Terzian, Ana Carolina Bernardes de Freitas Versiani, Alice Dibo, Margareth Regina Chiaravalotti-Neto, Francisco Weaver, Scott Cameron Estofolete, Cassia Fernanda Vasilakis, Nikos Nogueira, Mauricio Lacerda |
| description | Chikungunya virus (CHIKV) has spread across Brazil with varying incidence rates depending on the affected areas. Due to cocirculation of arboviruses and overlapping disease symptoms, CHIKV infection may be underdiagnosed. To understand the lack of CHIKV epidemics in São José do Rio Preto (SJdRP), São Paulo (SP), Brazil, we evaluated viral circulation by investigating anti-CHIKV IgG seroconversion in a prospective study of asymptomatic individuals and detecting anti-CHIKV IgM in individuals suspected of dengue infection, as well as CHIKV presence in Aedes mosquitoes. The opportunity to assess two different groups (symptomatic and asymptomatic) exposed at the same geographic region aimed to broaden the possibility of identifying the viral circulation, which had been previously considered absent.
Based on a prospective population study model and demographic characteristics (sex and age), we analyzed the anti-CHIKV IgG seroconversion rate in 341 subjects by ELISA over four years. The seroprevalence increased from 0.35% in the first year to 2.3% after 3 years of follow-up. Additionally, we investigated 497 samples from a blood panel collected from dengue-suspected individuals during the 2019 dengue outbreak in SJdRP. In total, 4.4% were positive for anti-CHIKV IgM, and 8.6% were positive for IgG. To exclude alphavirus cross-reactivity, we evaluated the presence of anti-Mayaro virus (MAYV) IgG by ELISA, and the positivity rate was 0.3% in the population study and 0.8% in the blood panel samples. In CHIKV and MAYV plaque reduction neutralization tests (PRNTs), the positivity rate for CHIKV-neutralizing antibodies in these ELISA-positive samples was 46.7%, while no MAYV-neutralizing antibodies were detected. Genomic sequencing and phylogenetic analysis revealed CHIKV genotype ECSA in São José do Rio Preto, SP. Finally, mosquitoes collected to complement human surveillance revealed CHIKV positivity of 2.76% of A. aegypti and 9.09% of A. albopictus (although it was far less abundant than A. aegypti) by RT-qPCR.
Our data suggest cryptic CHIKV circulation in SJdRP detected by continual active surveillance. These low levels, but increasing, of viral circulation highlight the possibility of CHIKV outbreaks, as there is a large naïve population. Improved knowledge of the epidemiological situation might aid in outbreaks prevention. |
| doi_str_mv | 10.1371/journal.pntd.0012013 |
| format | Article |
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Due to cocirculation of arboviruses and overlapping disease symptoms, CHIKV infection may be underdiagnosed. To understand the lack of CHIKV epidemics in São José do Rio Preto (SJdRP), São Paulo (SP), Brazil, we evaluated viral circulation by investigating anti-CHIKV IgG seroconversion in a prospective study of asymptomatic individuals and detecting anti-CHIKV IgM in individuals suspected of dengue infection, as well as CHIKV presence in Aedes mosquitoes. The opportunity to assess two different groups (symptomatic and asymptomatic) exposed at the same geographic region aimed to broaden the possibility of identifying the viral circulation, which had been previously considered absent.
Based on a prospective population study model and demographic characteristics (sex and age), we analyzed the anti-CHIKV IgG seroconversion rate in 341 subjects by ELISA over four years. The seroprevalence increased from 0.35% in the first year to 2.3% after 3 years of follow-up. Additionally, we investigated 497 samples from a blood panel collected from dengue-suspected individuals during the 2019 dengue outbreak in SJdRP. In total, 4.4% were positive for anti-CHIKV IgM, and 8.6% were positive for IgG. To exclude alphavirus cross-reactivity, we evaluated the presence of anti-Mayaro virus (MAYV) IgG by ELISA, and the positivity rate was 0.3% in the population study and 0.8% in the blood panel samples. In CHIKV and MAYV plaque reduction neutralization tests (PRNTs), the positivity rate for CHIKV-neutralizing antibodies in these ELISA-positive samples was 46.7%, while no MAYV-neutralizing antibodies were detected. Genomic sequencing and phylogenetic analysis revealed CHIKV genotype ECSA in São José do Rio Preto, SP. Finally, mosquitoes collected to complement human surveillance revealed CHIKV positivity of 2.76% of A. aegypti and 9.09% of A. albopictus (although it was far less abundant than A. aegypti) by RT-qPCR.
Our data suggest cryptic CHIKV circulation in SJdRP detected by continual active surveillance. These low levels, but increasing, of viral circulation highlight the possibility of CHIKV outbreaks, as there is a large naïve population. Improved knowledge of the epidemiological situation might aid in outbreaks prevention.</description><identifier>ISSN: 1935-2735</identifier><identifier>ISSN: 1935-2727</identifier><identifier>EISSN: 1935-2735</identifier><identifier>DOI: 10.1371/journal.pntd.0012013</identifier><identifier>PMID: 38484018</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Aedes ; Animals ; Antibodies ; Antibodies, Neutralizing - genetics ; Antibodies, Viral ; Aquatic insects ; Asymptomatic ; Biology and life sciences ; Blood ; Brazil ; Brazil - epidemiology ; Care and treatment ; Chikungunya fever ; Chikungunya Fever - epidemiology ; Chikungunya virus ; Chikungunya virus - genetics ; Circulation ; Consent ; Control ; Cross-reactivity ; Culicidae ; Dengue - diagnosis ; Dengue - epidemiology ; Dengue fever ; Diagnosis ; Epidemics ; Epidemiology ; Genomic analysis ; Genotypes ; Health surveillance ; Human diseases ; Humans ; Immunoglobulin G ; Immunoglobulin M ; Infections ; Medical research ; Medicine and Health Sciences ; Mosquitoes ; Neighborhoods ; Neutralization ; Neutralizing ; Outbreaks ; People and places ; Phylogenetics ; Phylogeny ; Population dynamics ; Population studies ; Prospective Studies ; Public health ; Questionnaires ; Research and Analysis Methods ; Review boards ; Risk factors ; Seroconversion ; Seroepidemiologic Studies ; Serology ; Signs and symptoms ; Sociodemographics ; Surveillance ; Vector-borne diseases ; Viral infections ; Viruses</subject><ispartof>PLoS neglected tropical diseases, 2024-03, Vol.18 (3), p.e0012013-e0012013</ispartof><rights>Copyright: © 2024 Zini et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.</rights><rights>COPYRIGHT 2024 Public Library of Science</rights><rights>2024 Zini et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2024 Zini et al 2024 Zini et al</rights><rights>2024 Zini et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c574t-78e2440f086a2ee40f358264bd16d8fae96109231d444576d757d4832891e8db3</cites><orcidid>0000-0003-1102-2419</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC10965090/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC10965090/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,724,777,781,861,882,2096,2915,23847,27905,27906,53772,53774,79349,79350</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/38484018$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Viennet, Elvina</contributor><creatorcontrib>Zini, Nathalia</creatorcontrib><creatorcontrib>Ávila, Matheus Henrique Tavares</creatorcontrib><creatorcontrib>Cezarotti, Natalia Morbi</creatorcontrib><creatorcontrib>Parra, Maisa Carla Pereira</creatorcontrib><creatorcontrib>Banho, Cecília Artico</creatorcontrib><creatorcontrib>Sacchetto, Livia</creatorcontrib><creatorcontrib>Negri, Andreia Francesli</creatorcontrib><creatorcontrib>Araújo, Emerson</creatorcontrib><creatorcontrib>Bittar, Cintia</creatorcontrib><creatorcontrib>Milhin, Bruno Henrique Gonçalves de Aguiar</creatorcontrib><creatorcontrib>Miranda Hernandes, Victor</creatorcontrib><creatorcontrib>Dutra, Karina Rocha</creatorcontrib><creatorcontrib>Trigo, Leonardo Agopian</creatorcontrib><creatorcontrib>Cecílio da Rocha, Leonardo</creatorcontrib><creatorcontrib>Alves da Silva, Rafael</creatorcontrib><creatorcontrib>Celestino Dutra da Silva, Gislaine</creatorcontrib><creatorcontrib>Fernanda Pereira Dos Santos, Tamires</creatorcontrib><creatorcontrib>de Carvalho Marques, Beatriz</creatorcontrib><creatorcontrib>Lopes Dos Santos, Andresa</creatorcontrib><creatorcontrib>Augusto, Marcos Tayar</creatorcontrib><creatorcontrib>Mistrão, Natalia Franco Bueno</creatorcontrib><creatorcontrib>Ribeiro, Milene Rocha</creatorcontrib><creatorcontrib>Pinheiro, Tauyne Menegaldo</creatorcontrib><creatorcontrib>Maria Izabel Lopes Dos Santos, Thayza</creatorcontrib><creatorcontrib>Avilla, Clarita Maria Secco</creatorcontrib><creatorcontrib>Bernardi, Victoria</creatorcontrib><creatorcontrib>Freitas, Caroline</creatorcontrib><creatorcontrib>Gandolfi, Flora de Andrade</creatorcontrib><creatorcontrib>Ferraz Júnior, Hélio Correa</creatorcontrib><creatorcontrib>Perim, Gabriela Camilotti</creatorcontrib><creatorcontrib>Gomes, Mirella Cezare</creatorcontrib><creatorcontrib>Garcia, Pedro Henrique Carrilho</creatorcontrib><creatorcontrib>Rocha, Rodrigo Sborghi</creatorcontrib><creatorcontrib>Galvão, Tayna Manfrin</creatorcontrib><creatorcontrib>Fávaro, Eliane Aparecida</creatorcontrib><creatorcontrib>Scamardi, Samuel Noah</creatorcontrib><creatorcontrib>Rogovski, Karen Sanmartin</creatorcontrib><creatorcontrib>Peixoto, Renan Luiz</creatorcontrib><creatorcontrib>Benfatti, Luiza</creatorcontrib><creatorcontrib>Cruz, Leonardo Teixeira</creatorcontrib><creatorcontrib>Chama, Paula Patricia de Freitas</creatorcontrib><creatorcontrib>Oliveira, Mânlio Tasso</creatorcontrib><creatorcontrib>Watanabe, Aripuanã Sakurada Aranha</creatorcontrib><creatorcontrib>Terzian, Ana Carolina Bernardes</creatorcontrib><creatorcontrib>de Freitas Versiani, Alice</creatorcontrib><creatorcontrib>Dibo, Margareth Regina</creatorcontrib><creatorcontrib>Chiaravalotti-Neto, Francisco</creatorcontrib><creatorcontrib>Weaver, Scott Cameron</creatorcontrib><creatorcontrib>Estofolete, Cassia Fernanda</creatorcontrib><creatorcontrib>Vasilakis, Nikos</creatorcontrib><creatorcontrib>Nogueira, Mauricio Lacerda</creatorcontrib><title>Cryptic circulation of chikungunya virus in São Jose do Rio Preto, Brazil, 2015-2019</title><title>PLoS neglected tropical diseases</title><addtitle>PLoS Negl Trop Dis</addtitle><description>Chikungunya virus (CHIKV) has spread across Brazil with varying incidence rates depending on the affected areas. Due to cocirculation of arboviruses and overlapping disease symptoms, CHIKV infection may be underdiagnosed. To understand the lack of CHIKV epidemics in São José do Rio Preto (SJdRP), São Paulo (SP), Brazil, we evaluated viral circulation by investigating anti-CHIKV IgG seroconversion in a prospective study of asymptomatic individuals and detecting anti-CHIKV IgM in individuals suspected of dengue infection, as well as CHIKV presence in Aedes mosquitoes. The opportunity to assess two different groups (symptomatic and asymptomatic) exposed at the same geographic region aimed to broaden the possibility of identifying the viral circulation, which had been previously considered absent.
Based on a prospective population study model and demographic characteristics (sex and age), we analyzed the anti-CHIKV IgG seroconversion rate in 341 subjects by ELISA over four years. The seroprevalence increased from 0.35% in the first year to 2.3% after 3 years of follow-up. Additionally, we investigated 497 samples from a blood panel collected from dengue-suspected individuals during the 2019 dengue outbreak in SJdRP. In total, 4.4% were positive for anti-CHIKV IgM, and 8.6% were positive for IgG. To exclude alphavirus cross-reactivity, we evaluated the presence of anti-Mayaro virus (MAYV) IgG by ELISA, and the positivity rate was 0.3% in the population study and 0.8% in the blood panel samples. In CHIKV and MAYV plaque reduction neutralization tests (PRNTs), the positivity rate for CHIKV-neutralizing antibodies in these ELISA-positive samples was 46.7%, while no MAYV-neutralizing antibodies were detected. Genomic sequencing and phylogenetic analysis revealed CHIKV genotype ECSA in São José do Rio Preto, SP. Finally, mosquitoes collected to complement human surveillance revealed CHIKV positivity of 2.76% of A. aegypti and 9.09% of A. albopictus (although it was far less abundant than A. aegypti) by RT-qPCR.
Our data suggest cryptic CHIKV circulation in SJdRP detected by continual active surveillance. These low levels, but increasing, of viral circulation highlight the possibility of CHIKV outbreaks, as there is a large naïve population. Improved knowledge of the epidemiological situation might aid in outbreaks prevention.</description><subject>Aedes</subject><subject>Animals</subject><subject>Antibodies</subject><subject>Antibodies, Neutralizing - genetics</subject><subject>Antibodies, Viral</subject><subject>Aquatic insects</subject><subject>Asymptomatic</subject><subject>Biology and life sciences</subject><subject>Blood</subject><subject>Brazil</subject><subject>Brazil - epidemiology</subject><subject>Care and treatment</subject><subject>Chikungunya fever</subject><subject>Chikungunya Fever - epidemiology</subject><subject>Chikungunya virus</subject><subject>Chikungunya virus - genetics</subject><subject>Circulation</subject><subject>Consent</subject><subject>Control</subject><subject>Cross-reactivity</subject><subject>Culicidae</subject><subject>Dengue - diagnosis</subject><subject>Dengue - epidemiology</subject><subject>Dengue fever</subject><subject>Diagnosis</subject><subject>Epidemics</subject><subject>Epidemiology</subject><subject>Genomic analysis</subject><subject>Genotypes</subject><subject>Health surveillance</subject><subject>Human diseases</subject><subject>Humans</subject><subject>Immunoglobulin G</subject><subject>Immunoglobulin M</subject><subject>Infections</subject><subject>Medical research</subject><subject>Medicine and Health Sciences</subject><subject>Mosquitoes</subject><subject>Neighborhoods</subject><subject>Neutralization</subject><subject>Neutralizing</subject><subject>Outbreaks</subject><subject>People and places</subject><subject>Phylogenetics</subject><subject>Phylogeny</subject><subject>Population dynamics</subject><subject>Population studies</subject><subject>Prospective Studies</subject><subject>Public health</subject><subject>Questionnaires</subject><subject>Research and Analysis Methods</subject><subject>Review boards</subject><subject>Risk factors</subject><subject>Seroconversion</subject><subject>Seroepidemiologic Studies</subject><subject>Serology</subject><subject>Signs and symptoms</subject><subject>Sociodemographics</subject><subject>Surveillance</subject><subject>Vector-borne diseases</subject><subject>Viral 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circulation of chikungunya virus in São Jose do Rio Preto, Brazil, 2015-2019</title><author>Zini, Nathalia ; Ávila, Matheus Henrique Tavares ; Cezarotti, Natalia Morbi ; Parra, Maisa Carla Pereira ; Banho, Cecília Artico ; Sacchetto, Livia ; Negri, Andreia Francesli ; Araújo, Emerson ; Bittar, Cintia ; Milhin, Bruno Henrique Gonçalves de Aguiar ; Miranda Hernandes, Victor ; Dutra, Karina Rocha ; Trigo, Leonardo Agopian ; Cecílio da Rocha, Leonardo ; Alves da Silva, Rafael ; Celestino Dutra da Silva, Gislaine ; Fernanda Pereira Dos Santos, Tamires ; de Carvalho Marques, Beatriz ; Lopes Dos Santos, Andresa ; Augusto, Marcos Tayar ; Mistrão, Natalia Franco Bueno ; Ribeiro, Milene Rocha ; Pinheiro, Tauyne Menegaldo ; Maria Izabel Lopes Dos Santos, Thayza ; Avilla, Clarita Maria Secco ; Bernardi, Victoria ; Freitas, Caroline ; Gandolfi, Flora de Andrade ; Ferraz Júnior, Hélio Correa ; Perim, Gabriela Camilotti ; Gomes, Mirella Cezare ; Garcia, Pedro Henrique Carrilho ; Rocha, Rodrigo Sborghi ; Galvão, Tayna Manfrin ; Fávaro, Eliane Aparecida ; Scamardi, Samuel Noah ; Rogovski, Karen Sanmartin ; Peixoto, Renan Luiz ; Benfatti, Luiza ; Cruz, Leonardo Teixeira ; Chama, Paula Patricia de Freitas ; Oliveira, Mânlio Tasso ; Watanabe, Aripuanã Sakurada Aranha ; Terzian, Ana Carolina Bernardes ; de Freitas Versiani, Alice ; Dibo, Margareth Regina ; Chiaravalotti-Neto, Francisco ; Weaver, Scott Cameron ; Estofolete, Cassia Fernanda ; Vasilakis, Nikos ; Nogueira, Mauricio Lacerda</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c574t-78e2440f086a2ee40f358264bd16d8fae96109231d444576d757d4832891e8db3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Aedes</topic><topic>Animals</topic><topic>Antibodies</topic><topic>Antibodies, Neutralizing - genetics</topic><topic>Antibodies, Viral</topic><topic>Aquatic insects</topic><topic>Asymptomatic</topic><topic>Biology and life sciences</topic><topic>Blood</topic><topic>Brazil</topic><topic>Brazil - epidemiology</topic><topic>Care and treatment</topic><topic>Chikungunya fever</topic><topic>Chikungunya Fever - epidemiology</topic><topic>Chikungunya virus</topic><topic>Chikungunya virus - genetics</topic><topic>Circulation</topic><topic>Consent</topic><topic>Control</topic><topic>Cross-reactivity</topic><topic>Culicidae</topic><topic>Dengue - diagnosis</topic><topic>Dengue - epidemiology</topic><topic>Dengue fever</topic><topic>Diagnosis</topic><topic>Epidemics</topic><topic>Epidemiology</topic><topic>Genomic analysis</topic><topic>Genotypes</topic><topic>Health surveillance</topic><topic>Human diseases</topic><topic>Humans</topic><topic>Immunoglobulin G</topic><topic>Immunoglobulin M</topic><topic>Infections</topic><topic>Medical research</topic><topic>Medicine and Health Sciences</topic><topic>Mosquitoes</topic><topic>Neighborhoods</topic><topic>Neutralization</topic><topic>Neutralizing</topic><topic>Outbreaks</topic><topic>People and places</topic><topic>Phylogenetics</topic><topic>Phylogeny</topic><topic>Population dynamics</topic><topic>Population studies</topic><topic>Prospective Studies</topic><topic>Public health</topic><topic>Questionnaires</topic><topic>Research and Analysis Methods</topic><topic>Review boards</topic><topic>Risk factors</topic><topic>Seroconversion</topic><topic>Seroepidemiologic Studies</topic><topic>Serology</topic><topic>Signs and symptoms</topic><topic>Sociodemographics</topic><topic>Surveillance</topic><topic>Vector-borne diseases</topic><topic>Viral infections</topic><topic>Viruses</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zini, Nathalia</creatorcontrib><creatorcontrib>Ávila, Matheus Henrique Tavares</creatorcontrib><creatorcontrib>Cezarotti, Natalia 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Tayar</creatorcontrib><creatorcontrib>Mistrão, Natalia Franco Bueno</creatorcontrib><creatorcontrib>Ribeiro, Milene Rocha</creatorcontrib><creatorcontrib>Pinheiro, Tauyne Menegaldo</creatorcontrib><creatorcontrib>Maria Izabel Lopes Dos Santos, Thayza</creatorcontrib><creatorcontrib>Avilla, Clarita Maria Secco</creatorcontrib><creatorcontrib>Bernardi, Victoria</creatorcontrib><creatorcontrib>Freitas, Caroline</creatorcontrib><creatorcontrib>Gandolfi, Flora de Andrade</creatorcontrib><creatorcontrib>Ferraz Júnior, Hélio Correa</creatorcontrib><creatorcontrib>Perim, Gabriela Camilotti</creatorcontrib><creatorcontrib>Gomes, Mirella Cezare</creatorcontrib><creatorcontrib>Garcia, Pedro Henrique Carrilho</creatorcontrib><creatorcontrib>Rocha, Rodrigo Sborghi</creatorcontrib><creatorcontrib>Galvão, Tayna Manfrin</creatorcontrib><creatorcontrib>Fávaro, Eliane Aparecida</creatorcontrib><creatorcontrib>Scamardi, Samuel Noah</creatorcontrib><creatorcontrib>Rogovski, Karen Sanmartin</creatorcontrib><creatorcontrib>Peixoto, Renan Luiz</creatorcontrib><creatorcontrib>Benfatti, Luiza</creatorcontrib><creatorcontrib>Cruz, Leonardo Teixeira</creatorcontrib><creatorcontrib>Chama, Paula Patricia de Freitas</creatorcontrib><creatorcontrib>Oliveira, Mânlio Tasso</creatorcontrib><creatorcontrib>Watanabe, Aripuanã Sakurada Aranha</creatorcontrib><creatorcontrib>Terzian, Ana Carolina Bernardes</creatorcontrib><creatorcontrib>de Freitas Versiani, Alice</creatorcontrib><creatorcontrib>Dibo, Margareth Regina</creatorcontrib><creatorcontrib>Chiaravalotti-Neto, Francisco</creatorcontrib><creatorcontrib>Weaver, Scott Cameron</creatorcontrib><creatorcontrib>Estofolete, Cassia Fernanda</creatorcontrib><creatorcontrib>Vasilakis, Nikos</creatorcontrib><creatorcontrib>Nogueira, Mauricio Lacerda</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE 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Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zini, Nathalia</au><au>Ávila, Matheus Henrique Tavares</au><au>Cezarotti, Natalia Morbi</au><au>Parra, Maisa Carla Pereira</au><au>Banho, Cecília Artico</au><au>Sacchetto, Livia</au><au>Negri, Andreia Francesli</au><au>Araújo, Emerson</au><au>Bittar, Cintia</au><au>Milhin, Bruno Henrique Gonçalves de Aguiar</au><au>Miranda Hernandes, Victor</au><au>Dutra, Karina Rocha</au><au>Trigo, Leonardo Agopian</au><au>Cecílio da Rocha, Leonardo</au><au>Alves da Silva, Rafael</au><au>Celestino Dutra da Silva, Gislaine</au><au>Fernanda Pereira Dos Santos, Tamires</au><au>de Carvalho Marques, Beatriz</au><au>Lopes Dos Santos, Andresa</au><au>Augusto, Marcos Tayar</au><au>Mistrão, Natalia Franco Bueno</au><au>Ribeiro, Milene Rocha</au><au>Pinheiro, Tauyne Menegaldo</au><au>Maria Izabel Lopes Dos Santos, Thayza</au><au>Avilla, Clarita Maria Secco</au><au>Bernardi, Victoria</au><au>Freitas, Caroline</au><au>Gandolfi, Flora de Andrade</au><au>Ferraz Júnior, Hélio Correa</au><au>Perim, Gabriela Camilotti</au><au>Gomes, Mirella Cezare</au><au>Garcia, Pedro Henrique Carrilho</au><au>Rocha, Rodrigo Sborghi</au><au>Galvão, Tayna Manfrin</au><au>Fávaro, Eliane Aparecida</au><au>Scamardi, Samuel Noah</au><au>Rogovski, Karen Sanmartin</au><au>Peixoto, Renan Luiz</au><au>Benfatti, Luiza</au><au>Cruz, Leonardo Teixeira</au><au>Chama, Paula Patricia de Freitas</au><au>Oliveira, Mânlio Tasso</au><au>Watanabe, Aripuanã Sakurada Aranha</au><au>Terzian, Ana Carolina Bernardes</au><au>de Freitas Versiani, Alice</au><au>Dibo, Margareth Regina</au><au>Chiaravalotti-Neto, Francisco</au><au>Weaver, Scott Cameron</au><au>Estofolete, Cassia Fernanda</au><au>Vasilakis, Nikos</au><au>Nogueira, Mauricio Lacerda</au><au>Viennet, Elvina</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cryptic circulation of chikungunya virus in São Jose do Rio Preto, Brazil, 2015-2019</atitle><jtitle>PLoS neglected tropical diseases</jtitle><addtitle>PLoS Negl Trop Dis</addtitle><date>2024-03-01</date><risdate>2024</risdate><volume>18</volume><issue>3</issue><spage>e0012013</spage><epage>e0012013</epage><pages>e0012013-e0012013</pages><issn>1935-2735</issn><issn>1935-2727</issn><eissn>1935-2735</eissn><abstract>Chikungunya virus (CHIKV) has spread across Brazil with varying incidence rates depending on the affected areas. Due to cocirculation of arboviruses and overlapping disease symptoms, CHIKV infection may be underdiagnosed. To understand the lack of CHIKV epidemics in São José do Rio Preto (SJdRP), São Paulo (SP), Brazil, we evaluated viral circulation by investigating anti-CHIKV IgG seroconversion in a prospective study of asymptomatic individuals and detecting anti-CHIKV IgM in individuals suspected of dengue infection, as well as CHIKV presence in Aedes mosquitoes. The opportunity to assess two different groups (symptomatic and asymptomatic) exposed at the same geographic region aimed to broaden the possibility of identifying the viral circulation, which had been previously considered absent.
Based on a prospective population study model and demographic characteristics (sex and age), we analyzed the anti-CHIKV IgG seroconversion rate in 341 subjects by ELISA over four years. The seroprevalence increased from 0.35% in the first year to 2.3% after 3 years of follow-up. Additionally, we investigated 497 samples from a blood panel collected from dengue-suspected individuals during the 2019 dengue outbreak in SJdRP. In total, 4.4% were positive for anti-CHIKV IgM, and 8.6% were positive for IgG. To exclude alphavirus cross-reactivity, we evaluated the presence of anti-Mayaro virus (MAYV) IgG by ELISA, and the positivity rate was 0.3% in the population study and 0.8% in the blood panel samples. In CHIKV and MAYV plaque reduction neutralization tests (PRNTs), the positivity rate for CHIKV-neutralizing antibodies in these ELISA-positive samples was 46.7%, while no MAYV-neutralizing antibodies were detected. Genomic sequencing and phylogenetic analysis revealed CHIKV genotype ECSA in São José do Rio Preto, SP. Finally, mosquitoes collected to complement human surveillance revealed CHIKV positivity of 2.76% of A. aegypti and 9.09% of A. albopictus (although it was far less abundant than A. aegypti) by RT-qPCR.
Our data suggest cryptic CHIKV circulation in SJdRP detected by continual active surveillance. These low levels, but increasing, of viral circulation highlight the possibility of CHIKV outbreaks, as there is a large naïve population. Improved knowledge of the epidemiological situation might aid in outbreaks prevention.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>38484018</pmid><doi>10.1371/journal.pntd.0012013</doi><orcidid>https://orcid.org/0000-0003-1102-2419</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1935-2735 |
| ispartof | PLoS neglected tropical diseases, 2024-03, Vol.18 (3), p.e0012013-e0012013 |
| issn | 1935-2735 1935-2727 1935-2735 |
| language | eng |
| recordid | cdi_plos_journals_3069183964 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central Open Access; Public Library of Science (PLoS); PubMed Central |
| subjects | Aedes Animals Antibodies Antibodies, Neutralizing - genetics Antibodies, Viral Aquatic insects Asymptomatic Biology and life sciences Blood Brazil Brazil - epidemiology Care and treatment Chikungunya fever Chikungunya Fever - epidemiology Chikungunya virus Chikungunya virus - genetics Circulation Consent Control Cross-reactivity Culicidae Dengue - diagnosis Dengue - epidemiology Dengue fever Diagnosis Epidemics Epidemiology Genomic analysis Genotypes Health surveillance Human diseases Humans Immunoglobulin G Immunoglobulin M Infections Medical research Medicine and Health Sciences Mosquitoes Neighborhoods Neutralization Neutralizing Outbreaks People and places Phylogenetics Phylogeny Population dynamics Population studies Prospective Studies Public health Questionnaires Research and Analysis Methods Review boards Risk factors Seroconversion Seroepidemiologic Studies Serology Signs and symptoms Sociodemographics Surveillance Vector-borne diseases Viral infections Viruses |
| title | Cryptic circulation of chikungunya virus in São Jose do Rio Preto, Brazil, 2015-2019 |
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