Genetic diversity and drug sensitivity profile of Mycobacterium tuberculosis among children in Ethiopia
Worldwide, tuberculosis (TB) affects about one million children every year. The burden of the disease is higher in developing countries. However, there is limited information on the lineages and drug sensitivity patterns of Mycobacterium tuberculosis (M. tuberculosis) infecting children in these cou...
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| description | Worldwide, tuberculosis (TB) affects about one million children every year. The burden of the disease is higher in developing countries. However, there is limited information on the lineages and drug sensitivity patterns of Mycobacterium tuberculosis (M. tuberculosis) infecting children in these countries, including Ethiopia. Thus, this study aimed to characterize the different lineages of the M. tuberculosis complex causing childhood pulmonary tuberculosis and evaluate the drug-sensitivity patterns to the first-line anti-TB drugs.
A total of 54 stored cultures were used in this study. The region of difference 9 (RD9) based polymerase chain reaction (PCR) and spoligotyping were employed for the identification of the isolates at the species and lineages level respectively. Lineage identification was done by using the pre-existing database. Identification of clustering of the spoligotype patterns was by using the SPOLIDB3-based model. The result was retrieved by the most probable family format. Furthermore, the phenotypic, and genotypic drug-sensitivity test (DST) was performed using Mycobacterium Growth Indicator Tube (MGIT™ 960) and GenoTypeMTBDRplus assay respectively. Data analysis was done using SPSS version 27 software.
Spoligotyping produced 39 interpretable results for M. tuberculosis. The majority (74.4%) of them were clustered into 7 groups, while the rest (25.6%) were single. The Euro-American (EA) lineage was the predominant lineage (64.1%) followed by the East-African Indian (EAI) (30.8%) and M. Africanum (5.1%) lineages. The most predominant subtypes were SIT37 (15.4%), SIT149 (12.8%), SIT25 (7.7%), and SIT53 (7.7%). Furthermore, of the identified SITs, T1 and CAS families consisted of 38.5% and 28.2% of the lineages respectively. Drug susceptibility was 91.9% by phenotypic method and 97.4% by molecular assay. The overall prevalence of any resistance was 7.8% and there was a single MDR-TB.
Many of the isolates belong to the modern lineages (Euro American) representing the most common circulating strains in the country. More importantly, despites the tiny isolates tested, drug resistance is low. To fully describe the molecular epidemiology of MTBC lineages in children, we recommend a prospective large-scale study. |
| doi_str_mv | 10.1371/journal.pone.0284363 |
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A total of 54 stored cultures were used in this study. The region of difference 9 (RD9) based polymerase chain reaction (PCR) and spoligotyping were employed for the identification of the isolates at the species and lineages level respectively. Lineage identification was done by using the pre-existing database. Identification of clustering of the spoligotype patterns was by using the SPOLIDB3-based model. The result was retrieved by the most probable family format. Furthermore, the phenotypic, and genotypic drug-sensitivity test (DST) was performed using Mycobacterium Growth Indicator Tube (MGIT™ 960) and GenoTypeMTBDRplus assay respectively. Data analysis was done using SPSS version 27 software.
Spoligotyping produced 39 interpretable results for M. tuberculosis. The majority (74.4%) of them were clustered into 7 groups, while the rest (25.6%) were single. The Euro-American (EA) lineage was the predominant lineage (64.1%) followed by the East-African Indian (EAI) (30.8%) and M. Africanum (5.1%) lineages. The most predominant subtypes were SIT37 (15.4%), SIT149 (12.8%), SIT25 (7.7%), and SIT53 (7.7%). Furthermore, of the identified SITs, T1 and CAS families consisted of 38.5% and 28.2% of the lineages respectively. Drug susceptibility was 91.9% by phenotypic method and 97.4% by molecular assay. The overall prevalence of any resistance was 7.8% and there was a single MDR-TB.
Many of the isolates belong to the modern lineages (Euro American) representing the most common circulating strains in the country. More importantly, despites the tiny isolates tested, drug resistance is low. To fully describe the molecular epidemiology of MTBC lineages in children, we recommend a prospective large-scale study.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0284363</identifier><identifier>PMID: 37506094</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Age groups ; Analysis ; Antitubercular agents ; Biological diversity ; Biology and Life Sciences ; Child ; Children ; Clustering ; Consent ; Data analysis ; Developing countries ; Diagnosis ; Diseases ; Drug Resistance ; Drug therapy ; Epidemiology ; Ethics ; Ethiopia - epidemiology ; Genetic aspects ; Genetic diversity ; Genetic Variation ; Genotype ; Humans ; Laboratories ; LDCs ; Medicine and Health Sciences ; Mortality ; Mycobacterium tuberculosis ; Pediatrics ; People and Places ; Polymerase chain reaction ; Prospective Studies ; Research and Analysis Methods ; Sensitivity analysis ; Spoligotyping ; Statistical analysis ; Tuberculosis ; Tuberculosis - epidemiology</subject><ispartof>PloS one, 2023-07, Vol.18 (7), p.e0284363-e0284363</ispartof><rights>Copyright: © 2023 Mollalign et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.</rights><rights>COPYRIGHT 2023 Public Library of Science</rights><rights>2023 Mollalign et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2023 Mollalign et al 2023 Mollalign et al</rights><rights>2023 Mollalign et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c642t-d26a067512d7cf1a8d9886edd722ee84599b15c2128feec3acaf8464526ef87b3</cites><orcidid>0000-0002-4134-6329 ; 0000-0002-0166-682X ; 0000-0002-1918-0668</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC10381036/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC10381036/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,2096,2915,23845,27901,27902,53766,53768,79342,79343</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/37506094$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Mollalign, Hilina</creatorcontrib><creatorcontrib>Getahun, Muluwork</creatorcontrib><creatorcontrib>Diriba, Getu</creatorcontrib><creatorcontrib>Alemu, Ayinalem</creatorcontrib><creatorcontrib>Chala, Dawit</creatorcontrib><creatorcontrib>Tulu, Begna</creatorcontrib><creatorcontrib>Ameni, Gobena</creatorcontrib><title>Genetic diversity and drug sensitivity profile of Mycobacterium tuberculosis among children in Ethiopia</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Worldwide, tuberculosis (TB) affects about one million children every year. The burden of the disease is higher in developing countries. However, there is limited information on the lineages and drug sensitivity patterns of Mycobacterium tuberculosis (M. tuberculosis) infecting children in these countries, including Ethiopia. Thus, this study aimed to characterize the different lineages of the M. tuberculosis complex causing childhood pulmonary tuberculosis and evaluate the drug-sensitivity patterns to the first-line anti-TB drugs.
A total of 54 stored cultures were used in this study. The region of difference 9 (RD9) based polymerase chain reaction (PCR) and spoligotyping were employed for the identification of the isolates at the species and lineages level respectively. Lineage identification was done by using the pre-existing database. Identification of clustering of the spoligotype patterns was by using the SPOLIDB3-based model. The result was retrieved by the most probable family format. Furthermore, the phenotypic, and genotypic drug-sensitivity test (DST) was performed using Mycobacterium Growth Indicator Tube (MGIT™ 960) and GenoTypeMTBDRplus assay respectively. Data analysis was done using SPSS version 27 software.
Spoligotyping produced 39 interpretable results for M. tuberculosis. The majority (74.4%) of them were clustered into 7 groups, while the rest (25.6%) were single. The Euro-American (EA) lineage was the predominant lineage (64.1%) followed by the East-African Indian (EAI) (30.8%) and M. Africanum (5.1%) lineages. The most predominant subtypes were SIT37 (15.4%), SIT149 (12.8%), SIT25 (7.7%), and SIT53 (7.7%). Furthermore, of the identified SITs, T1 and CAS families consisted of 38.5% and 28.2% of the lineages respectively. Drug susceptibility was 91.9% by phenotypic method and 97.4% by molecular assay. The overall prevalence of any resistance was 7.8% and there was a single MDR-TB.
Many of the isolates belong to the modern lineages (Euro American) representing the most common circulating strains in the country. More importantly, despites the tiny isolates tested, drug resistance is low. To fully describe the molecular epidemiology of MTBC lineages in children, we recommend a prospective large-scale study.</description><subject>Age groups</subject><subject>Analysis</subject><subject>Antitubercular agents</subject><subject>Biological diversity</subject><subject>Biology and Life Sciences</subject><subject>Child</subject><subject>Children</subject><subject>Clustering</subject><subject>Consent</subject><subject>Data analysis</subject><subject>Developing countries</subject><subject>Diagnosis</subject><subject>Diseases</subject><subject>Drug Resistance</subject><subject>Drug therapy</subject><subject>Epidemiology</subject><subject>Ethics</subject><subject>Ethiopia - epidemiology</subject><subject>Genetic aspects</subject><subject>Genetic diversity</subject><subject>Genetic Variation</subject><subject>Genotype</subject><subject>Humans</subject><subject>Laboratories</subject><subject>LDCs</subject><subject>Medicine and Health Sciences</subject><subject>Mortality</subject><subject>Mycobacterium tuberculosis</subject><subject>Pediatrics</subject><subject>People and Places</subject><subject>Polymerase chain reaction</subject><subject>Prospective Studies</subject><subject>Research and Analysis Methods</subject><subject>Sensitivity analysis</subject><subject>Spoligotyping</subject><subject>Statistical analysis</subject><subject>Tuberculosis</subject><subject>Tuberculosis - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mollalign, Hilina</au><au>Getahun, Muluwork</au><au>Diriba, Getu</au><au>Alemu, Ayinalem</au><au>Chala, Dawit</au><au>Tulu, Begna</au><au>Ameni, Gobena</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Genetic diversity and drug sensitivity profile of Mycobacterium tuberculosis among children in Ethiopia</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2023-07-28</date><risdate>2023</risdate><volume>18</volume><issue>7</issue><spage>e0284363</spage><epage>e0284363</epage><pages>e0284363-e0284363</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Worldwide, tuberculosis (TB) affects about one million children every year. The burden of the disease is higher in developing countries. However, there is limited information on the lineages and drug sensitivity patterns of Mycobacterium tuberculosis (M. tuberculosis) infecting children in these countries, including Ethiopia. Thus, this study aimed to characterize the different lineages of the M. tuberculosis complex causing childhood pulmonary tuberculosis and evaluate the drug-sensitivity patterns to the first-line anti-TB drugs.
A total of 54 stored cultures were used in this study. The region of difference 9 (RD9) based polymerase chain reaction (PCR) and spoligotyping were employed for the identification of the isolates at the species and lineages level respectively. Lineage identification was done by using the pre-existing database. Identification of clustering of the spoligotype patterns was by using the SPOLIDB3-based model. The result was retrieved by the most probable family format. Furthermore, the phenotypic, and genotypic drug-sensitivity test (DST) was performed using Mycobacterium Growth Indicator Tube (MGIT™ 960) and GenoTypeMTBDRplus assay respectively. Data analysis was done using SPSS version 27 software.
Spoligotyping produced 39 interpretable results for M. tuberculosis. The majority (74.4%) of them were clustered into 7 groups, while the rest (25.6%) were single. The Euro-American (EA) lineage was the predominant lineage (64.1%) followed by the East-African Indian (EAI) (30.8%) and M. Africanum (5.1%) lineages. The most predominant subtypes were SIT37 (15.4%), SIT149 (12.8%), SIT25 (7.7%), and SIT53 (7.7%). Furthermore, of the identified SITs, T1 and CAS families consisted of 38.5% and 28.2% of the lineages respectively. Drug susceptibility was 91.9% by phenotypic method and 97.4% by molecular assay. The overall prevalence of any resistance was 7.8% and there was a single MDR-TB.
Many of the isolates belong to the modern lineages (Euro American) representing the most common circulating strains in the country. More importantly, despites the tiny isolates tested, drug resistance is low. To fully describe the molecular epidemiology of MTBC lineages in children, we recommend a prospective large-scale study.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>37506094</pmid><doi>10.1371/journal.pone.0284363</doi><tpages>e0284363</tpages><orcidid>https://orcid.org/0000-0002-4134-6329</orcidid><orcidid>https://orcid.org/0000-0002-0166-682X</orcidid><orcidid>https://orcid.org/0000-0002-1918-0668</orcidid><oa>free_for_read</oa></addata></record> |
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| language | eng |
| recordid | cdi_plos_journals_2843384250 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; Free Full-Text Journals in Chemistry; Public Library of Science (PLoS) |
| subjects | Age groups Analysis Antitubercular agents Biological diversity Biology and Life Sciences Child Children Clustering Consent Data analysis Developing countries Diagnosis Diseases Drug Resistance Drug therapy Epidemiology Ethics Ethiopia - epidemiology Genetic aspects Genetic diversity Genetic Variation Genotype Humans Laboratories LDCs Medicine and Health Sciences Mortality Mycobacterium tuberculosis Pediatrics People and Places Polymerase chain reaction Prospective Studies Research and Analysis Methods Sensitivity analysis Spoligotyping Statistical analysis Tuberculosis Tuberculosis - epidemiology |
| title | Genetic diversity and drug sensitivity profile of Mycobacterium tuberculosis among children in Ethiopia |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-09T19%3A15%3A49IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Genetic%20diversity%20and%20drug%20sensitivity%20profile%20of%20Mycobacterium%20tuberculosis%20among%20children%20in%20Ethiopia&rft.jtitle=PloS%20one&rft.au=Mollalign,%20Hilina&rft.date=2023-07-28&rft.volume=18&rft.issue=7&rft.spage=e0284363&rft.epage=e0284363&rft.pages=e0284363-e0284363&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0284363&rft_dat=%3Cgale_plos_%3EA758741371%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2843384250&rft_id=info:pmid/37506094&rft_galeid=A758741371&rft_doaj_id=oai_doaj_org_article_45ffd104312449378a6cb626b79b2cb8&rfr_iscdi=true |