A new DNA extraction method (HV-CTAB-PCI) for amplification of nuclear markers from open ocean-retrieved faeces of an herbivorous marine mammal, the dugong

Non-invasively collected faecal samples are an alternative source of DNA to tissue samples, that may be used in genetic studies of wildlife when direct sampling of animals is difficult. Although several faecal DNA extraction methods exist, their efficacy varies between species. Previous attempts to...

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Veröffentlicht in:	PloS one 2023-06, Vol.18 (6), p.e0278792-e0278792
Hauptverfasser:	Ooi, Vicky, McMichael, Lee, Hunter, Margaret E, Takoukam Kamla, Aristide, Lanyon, Janet M
Format:	Artikel
Sprache:	eng
Schlagworte:	Ammonium Ammonium bromides Amplification Analysis Animals Aquatic mammals Biology and Life Sciences Bromides Caniformia Cetacea Cetrimonium Cetyltrimethylammonium bromide Chemical analysis Chloroform Deoxyribonucleic acid DNA DNA, Mitochondrial - genetics DNA, Mitochondrial - metabolism Dugong Dugongs Earth Sciences Feces Genetic aspects Genomes Herbivores Herbivory Isopentyl alcohol Marine mammals Microsatellites Mitochondrial DNA Nucleotides Oceans and Seas Percutaneous Coronary Intervention Phenols Polymorphism Population genetics Population studies Research and analysis methods Sampling Seawater Single-nucleotide polymorphism Skin Success Water analysis Wildlife
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