Characterization of the pattern of expression of Gas1 in the kidney during postnatal development in the rat
Growth Arrest-Specific 1 (Gas1) is a pleiotropic protein with different functions, in the adult kidney Gas1 acts as an endogenous inhibitor of cell proliferation but it is also necessary for the maintenance and proliferation of Renal Progenitor Cells (RPC) during early development, thus it fulfills...
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| creator | Cetina-Palma, Andrea Namorado-Tónix, Carmen Rodríguez-Muñoz, Rafael Vergara, Paula Reyes-Sánchez, José Luis Segovia, José |
| description | Growth Arrest-Specific 1 (Gas1) is a pleiotropic protein with different functions, in the adult kidney Gas1 acts as an endogenous inhibitor of cell proliferation but it is also necessary for the maintenance and proliferation of Renal Progenitor Cells (RPC) during early development, thus it fulfills important functions in the adult kidney. However, it is not known whether or not Gas1 is expressed during postnatal development, a critical stage for renal maturation. For this reason, the main objective of this work was to characterize the expression pattern of Gas1 in the different regions of the kidney by immunofluorescence and Western blot analysis during the postnatal development of the rat. We found that Gas1 is present and has a differential expression pattern in the various regions of the nephron during postnatal development. We observed that the highest levels of expression of Gas1 occur in the adult, however, Gas1 is also expressed in RPC and interestingly, the expression of RPC markers such as the Neural cell adhesion molecule (NCAM) and Cluster of differentiation 24 (CD24) were found to have an inverse pattern of expression to Gas1 (decreases as the kidney matures) during postnatal renal maturation, this indicates a role for Gas1 in the regulation of renal cell proliferation at this stage of development. |
| doi_str_mv | 10.1371/journal.pone.0284816 |
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However, it is not known whether or not Gas1 is expressed during postnatal development, a critical stage for renal maturation. For this reason, the main objective of this work was to characterize the expression pattern of Gas1 in the different regions of the kidney by immunofluorescence and Western blot analysis during the postnatal development of the rat. We found that Gas1 is present and has a differential expression pattern in the various regions of the nephron during postnatal development. We observed that the highest levels of expression of Gas1 occur in the adult, however, Gas1 is also expressed in RPC and interestingly, the expression of RPC markers such as the Neural cell adhesion molecule (NCAM) and Cluster of differentiation 24 (CD24) were found to have an inverse pattern of expression to Gas1 (decreases as the kidney matures) during postnatal renal maturation, this indicates a role for Gas1 in the regulation of renal cell proliferation at this stage of development.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0284816</identifier><identifier>PMID: 37093844</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Analysis ; Animal experimentation ; Animals ; Antibodies ; Biology and Life Sciences ; Cell adhesion ; Cell adhesion molecules ; Cell Cycle Proteins - metabolism ; Cell growth ; Cell Proliferation ; Cells (biology) ; Developmental stages ; Epithelial Cells - metabolism ; Ethylenediaminetetraacetic acid ; Evaluation ; Gas1 protein ; GPI-Linked Proteins - metabolism ; Immunofluorescence ; Kidneys ; Laboratory animals ; Maturation ; Medicine and Health Sciences ; Nephrons - metabolism ; Neural cell adhesion molecule ; Neural stem cells ; Progenitor cells ; Proteins ; Rats ; Rattus ; Research and Analysis Methods ; Sodium ; Stem Cells - metabolism ; Urine</subject><ispartof>PloS one, 2023-04, Vol.18 (4), p.e0284816-e0284816</ispartof><rights>Copyright: © 2023 Cetina-Palma et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.</rights><rights>COPYRIGHT 2023 Public Library of Science</rights><rights>2023 Cetina-Palma et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2023 Cetina-Palma et al 2023 Cetina-Palma et al</rights><rights>2023 Cetina-Palma et al. 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Cetina-Palma, Andrea</au><au>Namorado-Tónix, Carmen</au><au>Rodríguez-Muñoz, Rafael</au><au>Vergara, Paula</au><au>Reyes-Sánchez, José Luis</au><au>Segovia, José</au><au>Corridon, Peter R.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Characterization of the pattern of expression of Gas1 in the kidney during postnatal development in the rat</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2023-04-24</date><risdate>2023</risdate><volume>18</volume><issue>4</issue><spage>e0284816</spage><epage>e0284816</epage><pages>e0284816-e0284816</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Growth Arrest-Specific 1 (Gas1) is a pleiotropic protein with different functions, in the adult kidney Gas1 acts as an endogenous inhibitor of cell proliferation but it is also necessary for the maintenance and proliferation of Renal Progenitor Cells (RPC) during early development, thus it fulfills important functions in the adult kidney. However, it is not known whether or not Gas1 is expressed during postnatal development, a critical stage for renal maturation. For this reason, the main objective of this work was to characterize the expression pattern of Gas1 in the different regions of the kidney by immunofluorescence and Western blot analysis during the postnatal development of the rat. We found that Gas1 is present and has a differential expression pattern in the various regions of the nephron during postnatal development. We observed that the highest levels of expression of Gas1 occur in the adult, however, Gas1 is also expressed in RPC and interestingly, the expression of RPC markers such as the Neural cell adhesion molecule (NCAM) and Cluster of differentiation 24 (CD24) were found to have an inverse pattern of expression to Gas1 (decreases as the kidney matures) during postnatal renal maturation, this indicates a role for Gas1 in the regulation of renal cell proliferation at this stage of development.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>37093844</pmid><doi>10.1371/journal.pone.0284816</doi><tpages>e0284816</tpages><orcidid>https://orcid.org/0000-0001-8215-4772</orcidid><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; DOAJ Directory of Open Access Journals; Public Library of Science (PLoS) Journals Open Access; EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Analysis Animal experimentation Animals Antibodies Biology and Life Sciences Cell adhesion Cell adhesion molecules Cell Cycle Proteins - metabolism Cell growth Cell Proliferation Cells (biology) Developmental stages Epithelial Cells - metabolism Ethylenediaminetetraacetic acid Evaluation Gas1 protein GPI-Linked Proteins - metabolism Immunofluorescence Kidneys Laboratory animals Maturation Medicine and Health Sciences Nephrons - metabolism Neural cell adhesion molecule Neural stem cells Progenitor cells Proteins Rats Rattus Research and Analysis Methods Sodium Stem Cells - metabolism Urine |
| title | Characterization of the pattern of expression of Gas1 in the kidney during postnatal development in the rat |
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