A field-wide assessment of differential expression profiling by high-throughput sequencing reveals widespread bias

We assess inferential quality in the field of differential expression profiling by high-throughput sequencing (HT-seq) based on analysis of datasets submitted from 2008 to 2020 to the NCBI GEO data repository. We take advantage of the parallel differential expression testing over thousands of genes,...

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Veröffentlicht in:	PLoS biology 2023-03, Vol.21 (3), p.e3002007-e3002007
Hauptverfasser:	Päll, Taavi, Luidalepp, Hannes, Tenson, Tanel, Maiväli, Ülo
Format:	Artikel
Sprache:	eng
Schlagworte:	Analysis Bias Biology and Life Sciences Computer and Information Sciences DNA sequencing Engineering and Technology Experiments Gene expression Gene Expression Profiling - methods Genes High-Throughput Nucleotide Sequencing Histograms Hypotheses Hypothesis testing Medical research Medicine and Health Sciences Meta Methods Next-generation sequencing Nucleotide sequencing People and Places Physical Sciences Quality assessment Regression analysis Reproducibility Research and Analysis Methods Sample Size Science Science Policy Sequence Analysis, RNA - methods Statistical analysis Statistical inference Statistical methods
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