The epithelial polarity genes frazzled and GUK-holder adjust morphogen gradients to coordinate changes in cell position with cell fate specification
Morphogenetic gradients specify distinct cell populations within tissues. Originally, morphogens were conceived as substances that act on a static field of cells, yet cells usually move during development. Thus, the way cell fates are defined in moving cells remains a significant and largely unsolve...
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| Veröffentlicht in: | PLoS biology 2023-03, Vol.21 (3), p.e3002021-e3002021 |
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| creator | Xue, Yongqiang Krishnan, Aravindan Chahda, Juan Sebastian Schweickart, Robert Allen Sousa-Neves, Rui Mizutani, Claudia Mieko |
| description | Morphogenetic gradients specify distinct cell populations within tissues. Originally, morphogens were conceived as substances that act on a static field of cells, yet cells usually move during development. Thus, the way cell fates are defined in moving cells remains a significant and largely unsolved problem. Here, we investigated this issue using spatial referencing of cells and 3D spatial statistics in the Drosophila blastoderm to reveal how cell density responds to morphogenetic activity. We show that the morphogen decapentaplegic (DPP) attracts cells towards its peak levels in the dorsal midline, whereas dorsal (DL) stalls them ventrally. We identified frazzled and GUK-holder as the downstream effectors regulated by these morphogens that constrict cells and provide the mechanical force necessary to draw cells dorsally. Surprisingly, GUKH and FRA modulate the DL and DPP gradient levels and this regulation creates a very precise mechanism of coordinating cell movement and fate specification. |
| doi_str_mv | 10.1371/JOURNAL.PBIO.3002021 |
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Originally, morphogens were conceived as substances that act on a static field of cells, yet cells usually move during development. Thus, the way cell fates are defined in moving cells remains a significant and largely unsolved problem. Here, we investigated this issue using spatial referencing of cells and 3D spatial statistics in the Drosophila blastoderm to reveal how cell density responds to morphogenetic activity. We show that the morphogen decapentaplegic (DPP) attracts cells towards its peak levels in the dorsal midline, whereas dorsal (DL) stalls them ventrally. We identified frazzled and GUK-holder as the downstream effectors regulated by these morphogens that constrict cells and provide the mechanical force necessary to draw cells dorsally. 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| subjects | Analysis Animals Biology and Life Sciences Blastoderm Cell density Cell Differentiation Cell fate Cell migration Cells Computer and Information Sciences Drosophila Drosophila - genetics Drosophila melanogaster - genetics Drosophila Proteins - genetics Embryos Genetic aspects Identification and classification Metadata Morphogenesis Morphogenesis - genetics Nervous system Polarity Research and Analysis Methods Spatial analysis (Statistics) Specifications |
| title | The epithelial polarity genes frazzled and GUK-holder adjust morphogen gradients to coordinate changes in cell position with cell fate specification |
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