CRISPR/Cas9-Mediated genomic knock out of tyrosine hydroxylase and yellow genes in cricket Gryllus bimaculatus

Gryllus bimaculatus is an emerging model organism in various fields of biology such as behavior, neurology, physiology and genetics. Recently, application of reverse genetics provides an opportunity of understanding the functional genomics and manipulating gene regulation networks with specific phys...

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Veröffentlicht in:	PloS one 2023-04, Vol.18 (4), p.e0284124-e0284124
Hauptverfasser:	Bai, Yun, He, Yuan, Shen, Chu-Ze, Li, Kai, Li, Dong-Liang, He, Zhu-Qing
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Biology and Life Sciences Biosynthesis Catecholamine Catecholamines Conversion Crickets CRISPR CRISPR-Cas Systems - genetics Defects Dihydroxyphenylalanine Dopachrome isomerase Dopamine Efficiency Eggs Embryogenesis Embryonic development Embryonic growth stage Enzymes Evolution Gene deletion Gene regulation Genes Genetic aspects Genetic regulation Genetics Genomes Genomics Gryllidae Gryllus bimaculatus Homozygotes Humidity Hybridization Hydroxylases Insects Melanin Melanins - genetics Melanins - metabolism Mutants Mutation Neurology Nucleotides Phenols Phenotypes Physical Sciences Physiological aspects Physiology Pigmentation Proteins Research and analysis methods Tyrosine Tyrosine 3-monooxygenase Tyrosine 3-Monooxygenase - genetics Tyrosine 3-Monooxygenase - metabolism Wings Y gene Yellow protein
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