Identification and characterization of gut-associated lactic acid bacteria isolated from the bean bug, Riptortus pedestris (Hemiptera: Alydidae)
Lactic acid bacteria (LAB) are beneficial bacteria for humans and animals. However, the characteristics and functions of LAB in insects remain unclear. Here, we isolated LAB from the gut of Riptortus pedestris, a pest that is a significant problem in soybean cultivation in Korea, and identified two...
Gespeichert in:
| Veröffentlicht in: | PloS one 2023-03, Vol.18 (3), p.e0281121-e0281121 |
|---|---|
| Hauptverfasser: | , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | e0281121 |
|---|---|
| container_issue | 3 |
| container_start_page | e0281121 |
| container_title | PloS one |
| container_volume | 18 |
| creator | Choi, Okhee Lee, Yeyeong Kang, Byeongsam Cho, Su Kyung Kang, Yongsung Kang, Dong-Wan Lee, Seul-Bi Bae, Sung-Mun Kim, Jinwoo |
| description | Lactic acid bacteria (LAB) are beneficial bacteria for humans and animals. However, the characteristics and functions of LAB in insects remain unclear. Here, we isolated LAB from the gut of Riptortus pedestris, a pest that is a significant problem in soybean cultivation in Korea, and identified two Lactococcus lactis and one Enterococcus faecalis using matrix-associated laser desorption/ionization-time of flight and 16S rRNA analyses. All three LAB strains survived at pH 8, and L. lactis B103 and E. faecalis B105 survived at pH 9 for 24 h. In addition, these strains survived well in simulated gastric juice of humans containing pepsin and exhibited high resistance to bile salts. Two strains of L. lactis and one of E. faecalis maintained constant density (> 104 colony-forming units [CFU]/mL) at pH 2.5, but viability at pH 2.2 was strain-dependent. The three LAB were reinoculated into second-instar nymphs of R. pedestris and colonized well, reaching a constant density (> 105 CFU/gut) in the adult insect gut. Interestingly, feeding of these LAB increased the survival rate of insects compared to the negative control, with the largest increase seen for L. lactis B103. However, the LAB did not increase the weight or length of adult insects. These results indicate that insect-derived LAB possess the traits required for survival under gastrointestinal conditions and have beneficial effects on insect hosts. The LAB infection frequency of the wild bean bug populations was 89% (n = 18) in Gyeongsangnam-do, South Korea. These LAB can be utilized as a novel probiotic in the cultivation of beneficial insects. This study provides fundamental information about the symbiosis between insects and LAB, and a novel concept for pest control. |
| doi_str_mv | 10.1371/journal.pone.0281121 |
| format | Article |
| fullrecord | <record><control><sourceid>gale_plos_</sourceid><recordid>TN_cdi_plos_journals_2792882761</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A743645985</galeid><doaj_id>oai_doaj_org_article_28d86d4a50d3472f99ddc0b6c328de9c</doaj_id><sourcerecordid>A743645985</sourcerecordid><originalsourceid>FETCH-LOGICAL-c693t-a79f81f9787443dd4144495baadd552c830d68234ad65e345fbd66055a9d47a73</originalsourceid><addsrcrecordid>eNqNk11rFDEUhgdRbK3-A9GAIC24a74mM_FGlqJ2oVCoH7fhTJKZTZmdbJMZsf4Kf7LZ7rTsSC9kLjKc85w3yZtzsuwlwXPCCvL-yg-hg3a-8Z2dY1oSQsmj7JBIRmeCYvZ47_8gexbjFcY5K4V4mh0wIaXAjB9mf5bGdr2rnYbe-Q5BZ5BeQQDd2-B-74K-Rs3QzyBGrx301qA25Z1GoJ1B1Y4F5KJvb7N18GvUryyqLHSoGpp36NJteh_6IaKNNTb2wUV0fGbXKWwDfECL9sY4A_bkefakhjbaF-N6lH3__Onb6dns_OLL8nRxPtNCsnSWQtYlqWVRFpwzYzjhnMu8AjAmz6kuGTaipIyDEbllPK8rIwTOc5CGF1Cwo-z1TnfT-qhGM6OihaRlSQtBErHcEcbDldoEt4Zwozw4dRvwoVEQkgutVbQ0pTAccmwYL2gtpTEaV0KzlLFSJ62P425DtbZGJ88DtBPRaaZzK9X4n4pgLKjAIikcjwrBXw_JQbV2Udu2hc76YXdwiUtS0IS--Qd9-Hoj1UC6getqnzbWW1G1KDgTPJdlnqj5A1T6THo7nVqvdik-KTiZFCSmt7_6BoYY1fLr5f-zFz-m7Ns9dmWh7Vep34Ztg8YpyHegDj7GYOt7lwlW28m5c0NtJ0eNk5PKXu2_0H3R3aiwvz_YFO4</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2792882761</pqid></control><display><type>article</type><title>Identification and characterization of gut-associated lactic acid bacteria isolated from the bean bug, Riptortus pedestris (Hemiptera: Alydidae)</title><source>MEDLINE</source><source>DOAJ Directory of Open Access Journals</source><source>Public Library of Science (PLoS)</source><source>EZB-FREE-00999 freely available EZB journals</source><source>PubMed Central</source><source>Free Full-Text Journals in Chemistry</source><creator>Choi, Okhee ; Lee, Yeyeong ; Kang, Byeongsam ; Cho, Su Kyung ; Kang, Yongsung ; Kang, Dong-Wan ; Lee, Seul-Bi ; Bae, Sung-Mun ; Kim, Jinwoo</creator><contributor>Pietri, Jose</contributor><creatorcontrib>Choi, Okhee ; Lee, Yeyeong ; Kang, Byeongsam ; Cho, Su Kyung ; Kang, Yongsung ; Kang, Dong-Wan ; Lee, Seul-Bi ; Bae, Sung-Mun ; Kim, Jinwoo ; Pietri, Jose</creatorcontrib><description>Lactic acid bacteria (LAB) are beneficial bacteria for humans and animals. However, the characteristics and functions of LAB in insects remain unclear. Here, we isolated LAB from the gut of Riptortus pedestris, a pest that is a significant problem in soybean cultivation in Korea, and identified two Lactococcus lactis and one Enterococcus faecalis using matrix-associated laser desorption/ionization-time of flight and 16S rRNA analyses. All three LAB strains survived at pH 8, and L. lactis B103 and E. faecalis B105 survived at pH 9 for 24 h. In addition, these strains survived well in simulated gastric juice of humans containing pepsin and exhibited high resistance to bile salts. Two strains of L. lactis and one of E. faecalis maintained constant density (> 104 colony-forming units [CFU]/mL) at pH 2.5, but viability at pH 2.2 was strain-dependent. The three LAB were reinoculated into second-instar nymphs of R. pedestris and colonized well, reaching a constant density (> 105 CFU/gut) in the adult insect gut. Interestingly, feeding of these LAB increased the survival rate of insects compared to the negative control, with the largest increase seen for L. lactis B103. However, the LAB did not increase the weight or length of adult insects. These results indicate that insect-derived LAB possess the traits required for survival under gastrointestinal conditions and have beneficial effects on insect hosts. The LAB infection frequency of the wild bean bug populations was 89% (n = 18) in Gyeongsangnam-do, South Korea. These LAB can be utilized as a novel probiotic in the cultivation of beneficial insects. This study provides fundamental information about the symbiosis between insects and LAB, and a novel concept for pest control.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0281121</identifier><identifier>PMID: 36996034</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Acids ; Agricultural production ; Analysis ; Animals ; Bacteria ; Beans ; Bees ; Beneficial arthropods ; Biology and Life Sciences ; Cellulose acetate ; Cultivation ; Density ; Digestive system ; Digestive tract ; Fabaceae ; Gastrointestinal tract ; Glucose ; Glycine max ; Hemiptera ; Heteroptera - microbiology ; High resistance ; Humans ; Insects ; Ionization ; Lactic acid ; Lactic acid bacteria ; Lactobacillales ; Lasers ; Medicine and Health Sciences ; Microorganisms ; Pathogens ; Pepsin ; Pest control ; Pests ; pH effects ; Physical Sciences ; Probiotics ; Properties ; Riptortus pedestris ; RNA ; RNA, Ribosomal, 16S - genetics ; rRNA 16S ; Soybean ; Soybeans ; Strains (organisms) ; Survival ; Symbiosis</subject><ispartof>PloS one, 2023-03, Vol.18 (3), p.e0281121-e0281121</ispartof><rights>Copyright: © 2023 Choi et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.</rights><rights>COPYRIGHT 2023 Public Library of Science</rights><rights>2023 Choi et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2023 Choi et al 2023 Choi et al</rights><rights>2023 Choi et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c693t-a79f81f9787443dd4144495baadd552c830d68234ad65e345fbd66055a9d47a73</citedby><cites>FETCH-LOGICAL-c693t-a79f81f9787443dd4144495baadd552c830d68234ad65e345fbd66055a9d47a73</cites><orcidid>0000-0003-1104-8088</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC10062606/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC10062606/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,2095,2914,23846,27903,27904,53770,53772,79347,79348</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/36996034$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Pietri, Jose</contributor><creatorcontrib>Choi, Okhee</creatorcontrib><creatorcontrib>Lee, Yeyeong</creatorcontrib><creatorcontrib>Kang, Byeongsam</creatorcontrib><creatorcontrib>Cho, Su Kyung</creatorcontrib><creatorcontrib>Kang, Yongsung</creatorcontrib><creatorcontrib>Kang, Dong-Wan</creatorcontrib><creatorcontrib>Lee, Seul-Bi</creatorcontrib><creatorcontrib>Bae, Sung-Mun</creatorcontrib><creatorcontrib>Kim, Jinwoo</creatorcontrib><title>Identification and characterization of gut-associated lactic acid bacteria isolated from the bean bug, Riptortus pedestris (Hemiptera: Alydidae)</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Lactic acid bacteria (LAB) are beneficial bacteria for humans and animals. However, the characteristics and functions of LAB in insects remain unclear. Here, we isolated LAB from the gut of Riptortus pedestris, a pest that is a significant problem in soybean cultivation in Korea, and identified two Lactococcus lactis and one Enterococcus faecalis using matrix-associated laser desorption/ionization-time of flight and 16S rRNA analyses. All three LAB strains survived at pH 8, and L. lactis B103 and E. faecalis B105 survived at pH 9 for 24 h. In addition, these strains survived well in simulated gastric juice of humans containing pepsin and exhibited high resistance to bile salts. Two strains of L. lactis and one of E. faecalis maintained constant density (> 104 colony-forming units [CFU]/mL) at pH 2.5, but viability at pH 2.2 was strain-dependent. The three LAB were reinoculated into second-instar nymphs of R. pedestris and colonized well, reaching a constant density (> 105 CFU/gut) in the adult insect gut. Interestingly, feeding of these LAB increased the survival rate of insects compared to the negative control, with the largest increase seen for L. lactis B103. However, the LAB did not increase the weight or length of adult insects. These results indicate that insect-derived LAB possess the traits required for survival under gastrointestinal conditions and have beneficial effects on insect hosts. The LAB infection frequency of the wild bean bug populations was 89% (n = 18) in Gyeongsangnam-do, South Korea. These LAB can be utilized as a novel probiotic in the cultivation of beneficial insects. This study provides fundamental information about the symbiosis between insects and LAB, and a novel concept for pest control.</description><subject>Acids</subject><subject>Agricultural production</subject><subject>Analysis</subject><subject>Animals</subject><subject>Bacteria</subject><subject>Beans</subject><subject>Bees</subject><subject>Beneficial arthropods</subject><subject>Biology and Life Sciences</subject><subject>Cellulose acetate</subject><subject>Cultivation</subject><subject>Density</subject><subject>Digestive system</subject><subject>Digestive tract</subject><subject>Fabaceae</subject><subject>Gastrointestinal tract</subject><subject>Glucose</subject><subject>Glycine max</subject><subject>Hemiptera</subject><subject>Heteroptera - microbiology</subject><subject>High resistance</subject><subject>Humans</subject><subject>Insects</subject><subject>Ionization</subject><subject>Lactic acid</subject><subject>Lactic acid bacteria</subject><subject>Lactobacillales</subject><subject>Lasers</subject><subject>Medicine and Health Sciences</subject><subject>Microorganisms</subject><subject>Pathogens</subject><subject>Pepsin</subject><subject>Pest control</subject><subject>Pests</subject><subject>pH effects</subject><subject>Physical Sciences</subject><subject>Probiotics</subject><subject>Properties</subject><subject>Riptortus pedestris</subject><subject>RNA</subject><subject>RNA, Ribosomal, 16S - genetics</subject><subject>rRNA 16S</subject><subject>Soybean</subject><subject>Soybeans</subject><subject>Strains (organisms)</subject><subject>Survival</subject><subject>Symbiosis</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>DOA</sourceid><recordid>eNqNk11rFDEUhgdRbK3-A9GAIC24a74mM_FGlqJ2oVCoH7fhTJKZTZmdbJMZsf4Kf7LZ7rTsSC9kLjKc85w3yZtzsuwlwXPCCvL-yg-hg3a-8Z2dY1oSQsmj7JBIRmeCYvZ47_8gexbjFcY5K4V4mh0wIaXAjB9mf5bGdr2rnYbe-Q5BZ5BeQQDd2-B-74K-Rs3QzyBGrx301qA25Z1GoJ1B1Y4F5KJvb7N18GvUryyqLHSoGpp36NJteh_6IaKNNTb2wUV0fGbXKWwDfECL9sY4A_bkefakhjbaF-N6lH3__Onb6dns_OLL8nRxPtNCsnSWQtYlqWVRFpwzYzjhnMu8AjAmz6kuGTaipIyDEbllPK8rIwTOc5CGF1Cwo-z1TnfT-qhGM6OihaRlSQtBErHcEcbDldoEt4Zwozw4dRvwoVEQkgutVbQ0pTAccmwYL2gtpTEaV0KzlLFSJ62P425DtbZGJ88DtBPRaaZzK9X4n4pgLKjAIikcjwrBXw_JQbV2Udu2hc76YXdwiUtS0IS--Qd9-Hoj1UC6getqnzbWW1G1KDgTPJdlnqj5A1T6THo7nVqvdik-KTiZFCSmt7_6BoYY1fLr5f-zFz-m7Ns9dmWh7Vep34Ztg8YpyHegDj7GYOt7lwlW28m5c0NtJ0eNk5PKXu2_0H3R3aiwvz_YFO4</recordid><startdate>20230330</startdate><enddate>20230330</enddate><creator>Choi, Okhee</creator><creator>Lee, Yeyeong</creator><creator>Kang, Byeongsam</creator><creator>Cho, Su Kyung</creator><creator>Kang, Yongsung</creator><creator>Kang, Dong-Wan</creator><creator>Lee, Seul-Bi</creator><creator>Bae, Sung-Mun</creator><creator>Kim, Jinwoo</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0003-1104-8088</orcidid></search><sort><creationdate>20230330</creationdate><title>Identification and characterization of gut-associated lactic acid bacteria isolated from the bean bug, Riptortus pedestris (Hemiptera: Alydidae)</title><author>Choi, Okhee ; Lee, Yeyeong ; Kang, Byeongsam ; Cho, Su Kyung ; Kang, Yongsung ; Kang, Dong-Wan ; Lee, Seul-Bi ; Bae, Sung-Mun ; Kim, Jinwoo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c693t-a79f81f9787443dd4144495baadd552c830d68234ad65e345fbd66055a9d47a73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Acids</topic><topic>Agricultural production</topic><topic>Analysis</topic><topic>Animals</topic><topic>Bacteria</topic><topic>Beans</topic><topic>Bees</topic><topic>Beneficial arthropods</topic><topic>Biology and Life Sciences</topic><topic>Cellulose acetate</topic><topic>Cultivation</topic><topic>Density</topic><topic>Digestive system</topic><topic>Digestive tract</topic><topic>Fabaceae</topic><topic>Gastrointestinal tract</topic><topic>Glucose</topic><topic>Glycine max</topic><topic>Hemiptera</topic><topic>Heteroptera - microbiology</topic><topic>High resistance</topic><topic>Humans</topic><topic>Insects</topic><topic>Ionization</topic><topic>Lactic acid</topic><topic>Lactic acid bacteria</topic><topic>Lactobacillales</topic><topic>Lasers</topic><topic>Medicine and Health Sciences</topic><topic>Microorganisms</topic><topic>Pathogens</topic><topic>Pepsin</topic><topic>Pest control</topic><topic>Pests</topic><topic>pH effects</topic><topic>Physical Sciences</topic><topic>Probiotics</topic><topic>Properties</topic><topic>Riptortus pedestris</topic><topic>RNA</topic><topic>RNA, Ribosomal, 16S - genetics</topic><topic>rRNA 16S</topic><topic>Soybean</topic><topic>Soybeans</topic><topic>Strains (organisms)</topic><topic>Survival</topic><topic>Symbiosis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Choi, Okhee</creatorcontrib><creatorcontrib>Lee, Yeyeong</creatorcontrib><creatorcontrib>Kang, Byeongsam</creatorcontrib><creatorcontrib>Cho, Su Kyung</creatorcontrib><creatorcontrib>Kang, Yongsung</creatorcontrib><creatorcontrib>Kang, Dong-Wan</creatorcontrib><creatorcontrib>Lee, Seul-Bi</creatorcontrib><creatorcontrib>Bae, Sung-Mun</creatorcontrib><creatorcontrib>Kim, Jinwoo</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: Opposing Viewpoints</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Proquest Nursing & Allied Health Source</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Meteorological & Geoastrophysical Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Meteorological & Geoastrophysical Abstracts - Academic</collection><collection>ProQuest Engineering Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Engineering Database</collection><collection>Nursing & Allied Health Premium</collection><collection>Advanced Technologies & Aerospace Database</collection><collection>ProQuest Advanced Technologies & Aerospace Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>Materials Science Collection</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Engineering Collection</collection><collection>Environmental Science Collection</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Choi, Okhee</au><au>Lee, Yeyeong</au><au>Kang, Byeongsam</au><au>Cho, Su Kyung</au><au>Kang, Yongsung</au><au>Kang, Dong-Wan</au><au>Lee, Seul-Bi</au><au>Bae, Sung-Mun</au><au>Kim, Jinwoo</au><au>Pietri, Jose</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Identification and characterization of gut-associated lactic acid bacteria isolated from the bean bug, Riptortus pedestris (Hemiptera: Alydidae)</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2023-03-30</date><risdate>2023</risdate><volume>18</volume><issue>3</issue><spage>e0281121</spage><epage>e0281121</epage><pages>e0281121-e0281121</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Lactic acid bacteria (LAB) are beneficial bacteria for humans and animals. However, the characteristics and functions of LAB in insects remain unclear. Here, we isolated LAB from the gut of Riptortus pedestris, a pest that is a significant problem in soybean cultivation in Korea, and identified two Lactococcus lactis and one Enterococcus faecalis using matrix-associated laser desorption/ionization-time of flight and 16S rRNA analyses. All three LAB strains survived at pH 8, and L. lactis B103 and E. faecalis B105 survived at pH 9 for 24 h. In addition, these strains survived well in simulated gastric juice of humans containing pepsin and exhibited high resistance to bile salts. Two strains of L. lactis and one of E. faecalis maintained constant density (> 104 colony-forming units [CFU]/mL) at pH 2.5, but viability at pH 2.2 was strain-dependent. The three LAB were reinoculated into second-instar nymphs of R. pedestris and colonized well, reaching a constant density (> 105 CFU/gut) in the adult insect gut. Interestingly, feeding of these LAB increased the survival rate of insects compared to the negative control, with the largest increase seen for L. lactis B103. However, the LAB did not increase the weight or length of adult insects. These results indicate that insect-derived LAB possess the traits required for survival under gastrointestinal conditions and have beneficial effects on insect hosts. The LAB infection frequency of the wild bean bug populations was 89% (n = 18) in Gyeongsangnam-do, South Korea. These LAB can be utilized as a novel probiotic in the cultivation of beneficial insects. This study provides fundamental information about the symbiosis between insects and LAB, and a novel concept for pest control.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>36996034</pmid><doi>10.1371/journal.pone.0281121</doi><tpages>e0281121</tpages><orcidid>https://orcid.org/0000-0003-1104-8088</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2023-03, Vol.18 (3), p.e0281121-e0281121 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_2792882761 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Public Library of Science (PLoS); EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Acids Agricultural production Analysis Animals Bacteria Beans Bees Beneficial arthropods Biology and Life Sciences Cellulose acetate Cultivation Density Digestive system Digestive tract Fabaceae Gastrointestinal tract Glucose Glycine max Hemiptera Heteroptera - microbiology High resistance Humans Insects Ionization Lactic acid Lactic acid bacteria Lactobacillales Lasers Medicine and Health Sciences Microorganisms Pathogens Pepsin Pest control Pests pH effects Physical Sciences Probiotics Properties Riptortus pedestris RNA RNA, Ribosomal, 16S - genetics rRNA 16S Soybean Soybeans Strains (organisms) Survival Symbiosis |
| title | Identification and characterization of gut-associated lactic acid bacteria isolated from the bean bug, Riptortus pedestris (Hemiptera: Alydidae) |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-22T08%3A43%3A13IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Identification%20and%20characterization%20of%20gut-associated%20lactic%20acid%20bacteria%20isolated%20from%20the%20bean%20bug,%20Riptortus%20pedestris%20(Hemiptera:%20Alydidae)&rft.jtitle=PloS%20one&rft.au=Choi,%20Okhee&rft.date=2023-03-30&rft.volume=18&rft.issue=3&rft.spage=e0281121&rft.epage=e0281121&rft.pages=e0281121-e0281121&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0281121&rft_dat=%3Cgale_plos_%3EA743645985%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2792882761&rft_id=info:pmid/36996034&rft_galeid=A743645985&rft_doaj_id=oai_doaj_org_article_28d86d4a50d3472f99ddc0b6c328de9c&rfr_iscdi=true |