Development of a programmable magnetic agitation device to maintain colloidal suspension of cells during microfluidic syringe pump perfusion
Droplet-based microfluidic devices have been used to achieve homogeneous cell encapsulation, but cells sediment in a solution, leading to heterogeneous products. In this technical note, we describe automated and programmable agitation device to maintain colloidal suspensions of cells. We demonstrate...
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| Veröffentlicht in: | PloS one 2023-03, Vol.18 (3), p.e0282563-e0282563 |
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| creator | Puttrich, Tommy O'Donnell, Steven Wong, Sing-Wan Kotche, Miiri Felder, Anthony E Shin, Jae-Won |
| description | Droplet-based microfluidic devices have been used to achieve homogeneous cell encapsulation, but cells sediment in a solution, leading to heterogeneous products. In this technical note, we describe automated and programmable agitation device to maintain colloidal suspensions of cells. We demonstrate that the agitation device can be interfaced with a syringe pump for microfluidic applications. Agitation profiles of the device were predictable and corresponded to device settings. The device maintains the concentration of cells in an alginate solution over time without implicating cell viability. This device replaces manual agitation, and hence is suitable for applications that require slow perfusion for a longer period of time in a scalable manner. |
| doi_str_mv | 10.1371/journal.pone.0282563 |
| format | Article |
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In this technical note, we describe automated and programmable agitation device to maintain colloidal suspensions of cells. We demonstrate that the agitation device can be interfaced with a syringe pump for microfluidic applications. Agitation profiles of the device were predictable and corresponded to device settings. The device maintains the concentration of cells in an alginate solution over time without implicating cell viability. This device replaces manual agitation, and hence is suitable for applications that require slow perfusion for a longer period of time in a scalable manner.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0282563</identifier><identifier>PMID: 36888609</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Agitation ; Alginates ; Alginic acid ; Analysis ; Automation ; Biology and Life Sciences ; Cell culture ; Cell Survival ; Cell viability ; Colloids ; Computer and Information Sciences ; Electronic equipment and supplies ; Engineering and Technology ; LCDs ; Liquid crystal displays ; Magnetic Phenomena ; Methods ; Microfluidic devices ; Microfluidics ; Perfusion ; Physical Sciences ; Plastic embedment ; Research and Analysis Methods ; Syringes ; Tissue engineering ; Translations ; Velocity</subject><ispartof>PloS one, 2023-03, Vol.18 (3), p.e0282563-e0282563</ispartof><rights>Copyright: © 2023 Puttrich et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.</rights><rights>COPYRIGHT 2023 Public Library of Science</rights><rights>2023 Puttrich et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2023 Puttrich et al 2023 Puttrich et al</rights><rights>2023 Puttrich et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c692t-a0342b9e0dd5fb3c644dd70d967661cd77fe402935e3f113ce7b366abc8cc7fe3</citedby><cites>FETCH-LOGICAL-c692t-a0342b9e0dd5fb3c644dd70d967661cd77fe402935e3f113ce7b366abc8cc7fe3</cites><orcidid>0000-0003-4823-6373</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC9994697/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC9994697/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,315,729,782,786,866,887,2106,2932,23875,27933,27934,53800,53802</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/36888609$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Chalmers, Jeffrey</contributor><creatorcontrib>Puttrich, Tommy</creatorcontrib><creatorcontrib>O'Donnell, Steven</creatorcontrib><creatorcontrib>Wong, Sing-Wan</creatorcontrib><creatorcontrib>Kotche, Miiri</creatorcontrib><creatorcontrib>Felder, Anthony E</creatorcontrib><creatorcontrib>Shin, Jae-Won</creatorcontrib><title>Development of a programmable magnetic agitation device to maintain colloidal suspension of cells during microfluidic syringe pump perfusion</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Droplet-based microfluidic devices have been used to achieve homogeneous cell encapsulation, but cells sediment in a solution, leading to heterogeneous products. In this technical note, we describe automated and programmable agitation device to maintain colloidal suspensions of cells. We demonstrate that the agitation device can be interfaced with a syringe pump for microfluidic applications. Agitation profiles of the device were predictable and corresponded to device settings. The device maintains the concentration of cells in an alginate solution over time without implicating cell viability. 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| subjects | Agitation Alginates Alginic acid Analysis Automation Biology and Life Sciences Cell culture Cell Survival Cell viability Colloids Computer and Information Sciences Electronic equipment and supplies Engineering and Technology LCDs Liquid crystal displays Magnetic Phenomena Methods Microfluidic devices Microfluidics Perfusion Physical Sciences Plastic embedment Research and Analysis Methods Syringes Tissue engineering Translations Velocity |
| title | Development of a programmable magnetic agitation device to maintain colloidal suspension of cells during microfluidic syringe pump perfusion |
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