Drosophila melanogaster resilin improves the mechanical properties of transgenic silk
Resilin is a natural protein with high extensibility and resilience that plays a key role in the biological processes of insects, such as flight, bouncing, and vocalization. This study used piggyBac-mediated transgenic technology to stably insert the Drosophila melanogaster resilin gene into the sil...
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| Veröffentlicht in: | PloS one 2023-03, Vol.18 (3), p.e0282533-e0282533 |
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| creator | Zhao, Shuo Ye, Xiaogang Dai, Xiangping Wang, Xinqiu Yu, Shihua Zhong, Boxiong |
| description | Resilin is a natural protein with high extensibility and resilience that plays a key role in the biological processes of insects, such as flight, bouncing, and vocalization. This study used piggyBac-mediated transgenic technology to stably insert the Drosophila melanogaster resilin gene into the silkworm genome to investigate whether exogenous protein structures improve the mechanical properties of silkworm silk. Molecular detection showed that recombinant resilin was expressed and secreted into silk. Secondary structure and mechanical property analysis showed that the β-sheet content in silk from transgenic silkworms was higher than in wild-type silk. The fracture strength of silk fused with resilin protein was 7.2% higher than wild-type silk. The resilience of recombinant silk after one-time stretching and cyclic stretching was 20.5% and 18.7% higher than wild-type silk, respectively. In summary, Drosophila resilin can enhance the mechanical properties of silk, and this study is the first to improve the mechanical properties of silk using proteins other than spider silk, which broadens the possibilities for the design and application of biomimetic silk materials. |
| doi_str_mv | 10.1371/journal.pone.0282533 |
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This study used piggyBac-mediated transgenic technology to stably insert the Drosophila melanogaster resilin gene into the silkworm genome to investigate whether exogenous protein structures improve the mechanical properties of silkworm silk. Molecular detection showed that recombinant resilin was expressed and secreted into silk. Secondary structure and mechanical property analysis showed that the β-sheet content in silk from transgenic silkworms was higher than in wild-type silk. The fracture strength of silk fused with resilin protein was 7.2% higher than wild-type silk. The resilience of recombinant silk after one-time stretching and cyclic stretching was 20.5% and 18.7% higher than wild-type silk, respectively. In summary, Drosophila resilin can enhance the mechanical properties of silk, and this study is the first to improve the mechanical properties of silk using proteins other than spider silk, which broadens the possibilities for the design and application of biomimetic silk materials.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0282533</identifier><identifier>PMID: 36867637</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Animals ; Animals, Genetically Modified ; Antibodies ; Biological activity ; Biology and Life Sciences ; Biomimetic materials ; Biomimetics ; Bombyx ; Data smoothing ; Drosophila ; Drosophila melanogaster ; Fracture strength ; Fruit flies ; Genes ; Genomes ; Humidity ; Insect Proteins ; Insects ; Laboratories ; Mechanical properties ; Physical Sciences ; Polypeptides ; Properties ; Protein structure ; Proteins ; Research and Analysis Methods ; Resilience ; Secondary structure ; Silk ; Silkworms ; Spectrum analysis ; Stretching</subject><ispartof>PloS one, 2023-03, Vol.18 (3), p.e0282533-e0282533</ispartof><rights>Copyright: © 2023 Zhao et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.</rights><rights>COPYRIGHT 2023 Public Library of Science</rights><rights>2023 Zhao et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2023 Zhao et al 2023 Zhao et al</rights><rights>2023 Zhao et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c692t-6b9416a4e00fb993e63d822dc95738f58ddadecdb564340523fdab3a8df950f93</citedby><cites>FETCH-LOGICAL-c692t-6b9416a4e00fb993e63d822dc95738f58ddadecdb564340523fdab3a8df950f93</cites><orcidid>0000-0003-3917-5222 ; 0000-0002-6738-1747 ; 0000-0002-0087-8839</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC9983856/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC9983856/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,2100,2919,23857,27915,27916,53782,53784,79361,79362</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/36867637$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Xu, Jian</contributor><creatorcontrib>Zhao, Shuo</creatorcontrib><creatorcontrib>Ye, Xiaogang</creatorcontrib><creatorcontrib>Dai, Xiangping</creatorcontrib><creatorcontrib>Wang, Xinqiu</creatorcontrib><creatorcontrib>Yu, Shihua</creatorcontrib><creatorcontrib>Zhong, Boxiong</creatorcontrib><title>Drosophila melanogaster resilin improves the mechanical properties of transgenic silk</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Resilin is a natural protein with high extensibility and resilience that plays a key role in the biological processes of insects, such as flight, bouncing, and vocalization. This study used piggyBac-mediated transgenic technology to stably insert the Drosophila melanogaster resilin gene into the silkworm genome to investigate whether exogenous protein structures improve the mechanical properties of silkworm silk. Molecular detection showed that recombinant resilin was expressed and secreted into silk. Secondary structure and mechanical property analysis showed that the β-sheet content in silk from transgenic silkworms was higher than in wild-type silk. The fracture strength of silk fused with resilin protein was 7.2% higher than wild-type silk. The resilience of recombinant silk after one-time stretching and cyclic stretching was 20.5% and 18.7% higher than wild-type silk, respectively. In summary, Drosophila resilin can enhance the mechanical properties of silk, and this study is the first to improve the mechanical properties of silk using proteins other than spider silk, which broadens the possibilities for the design and application of biomimetic silk materials.</description><subject>Animals</subject><subject>Animals, Genetically Modified</subject><subject>Antibodies</subject><subject>Biological activity</subject><subject>Biology and Life Sciences</subject><subject>Biomimetic materials</subject><subject>Biomimetics</subject><subject>Bombyx</subject><subject>Data smoothing</subject><subject>Drosophila</subject><subject>Drosophila melanogaster</subject><subject>Fracture strength</subject><subject>Fruit flies</subject><subject>Genes</subject><subject>Genomes</subject><subject>Humidity</subject><subject>Insect Proteins</subject><subject>Insects</subject><subject>Laboratories</subject><subject>Mechanical properties</subject><subject>Physical 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This study used piggyBac-mediated transgenic technology to stably insert the Drosophila melanogaster resilin gene into the silkworm genome to investigate whether exogenous protein structures improve the mechanical properties of silkworm silk. Molecular detection showed that recombinant resilin was expressed and secreted into silk. Secondary structure and mechanical property analysis showed that the β-sheet content in silk from transgenic silkworms was higher than in wild-type silk. The fracture strength of silk fused with resilin protein was 7.2% higher than wild-type silk. The resilience of recombinant silk after one-time stretching and cyclic stretching was 20.5% and 18.7% higher than wild-type silk, respectively. In summary, Drosophila resilin can enhance the mechanical properties of silk, and this study is the first to improve the mechanical properties of silk using proteins other than spider silk, which broadens the possibilities for the design and application of biomimetic silk materials.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>36867637</pmid><doi>10.1371/journal.pone.0282533</doi><tpages>e0282533</tpages><orcidid>https://orcid.org/0000-0003-3917-5222</orcidid><orcidid>https://orcid.org/0000-0002-6738-1747</orcidid><orcidid>https://orcid.org/0000-0002-0087-8839</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Animals Animals, Genetically Modified Antibodies Biological activity Biology and Life Sciences Biomimetic materials Biomimetics Bombyx Data smoothing Drosophila Drosophila melanogaster Fracture strength Fruit flies Genes Genomes Humidity Insect Proteins Insects Laboratories Mechanical properties Physical Sciences Polypeptides Properties Protein structure Proteins Research and Analysis Methods Resilience Secondary structure Silk Silkworms Spectrum analysis Stretching |
| title | Drosophila melanogaster resilin improves the mechanical properties of transgenic silk |
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