Colonization of intervertebral discs by Cutibacterium acnes in patients with low back pain: Protocol for an analytical study with microbiological, phenotypic, genotypic, and multiomic techniques
Lumbar disc degeneration (LDD) and low back pain (LBP) are two conditions that are closely related. Several studies have shown Cutibacterium acnes colonization of degenerated discs, but whether and how these finding correlates with LBP is unknown. A prospective study was planned to identify molecule...
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| creator | Magno da Rocha, Vinícius Lima, Carla Ormundo Gonçalves Ximenes Ferreira, Eliane de Oliveira de Farias, Gabriel Corrêa Nogueira, Fábio César Sousa Martha Antunes, Luis Caetano Cassiano, Keila Mara Fiorelli, Rossano Kepler Alvim |
| description | Lumbar disc degeneration (LDD) and low back pain (LBP) are two conditions that are closely related. Several studies have shown Cutibacterium acnes colonization of degenerated discs, but whether and how these finding correlates with LBP is unknown. A prospective study was planned to identify molecules present in lumbar intervertebral discs (LLIVD) colonized by C. acnes in patients with LDD and LBP and correlate them with their clinical, radiological, and demographic profiles. The clinical manifestations, risk factors, and demographic characteristics of participants undergoing surgical microdiscectomy will be tracked. Samples will be isolated and pathogens found in LLIVD will be characterized phenotypically and genotypically. Whole genome sequencing (WGS) of isolated species will be used to phylotype and detect genes associated with virulence, resistance, and oxidative stress. Multiomic analyses of LLIVD colonized and non-colonized will be carried out to explain not only the pathogen's role in LDD, but also its involvement in the pathophysiology of LBP. This study was approved by the Institutional Review Board (CAAE 50077521.0.0000.5258). All patients who agree to participate in the study will sign an informed consent form. Regardless of the study's findings, the results will be published in a peer-reviewed medical journal. Trials registration number NCT05090553; pre-results. |
| doi_str_mv | 10.1371/journal.pone.0271773 |
| format | Article |
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William</contributor><creatorcontrib>Magno da Rocha, Vinícius ; Lima, Carla Ormundo Gonçalves Ximenes ; Ferreira, Eliane de Oliveira ; de Farias, Gabriel Corrêa ; Nogueira, Fábio César Sousa ; Martha Antunes, Luis Caetano ; Cassiano, Keila Mara ; Fiorelli, Rossano Kepler Alvim ; Cameron, D. William</creatorcontrib><description>Lumbar disc degeneration (LDD) and low back pain (LBP) are two conditions that are closely related. Several studies have shown Cutibacterium acnes colonization of degenerated discs, but whether and how these finding correlates with LBP is unknown. A prospective study was planned to identify molecules present in lumbar intervertebral discs (LLIVD) colonized by C. acnes in patients with LDD and LBP and correlate them with their clinical, radiological, and demographic profiles. The clinical manifestations, risk factors, and demographic characteristics of participants undergoing surgical microdiscectomy will be tracked. Samples will be isolated and pathogens found in LLIVD will be characterized phenotypically and genotypically. Whole genome sequencing (WGS) of isolated species will be used to phylotype and detect genes associated with virulence, resistance, and oxidative stress. Multiomic analyses of LLIVD colonized and non-colonized will be carried out to explain not only the pathogen's role in LDD, but also its involvement in the pathophysiology of LBP. This study was approved by the Institutional Review Board (CAAE 50077521.0.0000.5258). All patients who agree to participate in the study will sign an informed consent form. Regardless of the study's findings, the results will be published in a peer-reviewed medical journal. Trials registration number NCT05090553; pre-results.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0271773</identifier><identifier>PMID: 36848344</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Analysis ; Antibiotics ; Back pain ; Back surgery ; Biofilms ; Biology and Life Sciences ; Care and treatment ; Causes of ; Clinical trials ; Colonization ; Computer and Information Sciences ; Control ; Cutibacterium acnes ; Degeneration ; Demographics ; Demography ; Diagnosis ; Engineering and Technology ; Gene sequencing ; Genomes ; Hernia ; Humans ; Hypotheses ; Identification and classification ; Informed consent ; Intervertebral Disc ; Intervertebral Disc Degeneration - genetics ; Intervertebral discs ; Intervertebral disk ; Intervertebral disk displacement ; Laboratories ; Low back pain ; Low Back Pain - genetics ; Medicine and Health Sciences ; Multiomics ; Oxidation resistance ; Oxidative stress ; Pain ; Pathogenic microorganisms ; Pathogens ; Patients ; Physical Sciences ; Propionibacterium acnes - genetics ; Prospective Studies ; Prostheses ; Proteins ; Research and Analysis Methods ; Review boards ; Risk factors ; Study Protocol ; Virulence ; Virulence (Microbiology) ; Whole genome sequencing</subject><ispartof>PloS one, 2023-02, Vol.18 (2), p.e0271773-e0271773</ispartof><rights>Copyright: © 2023 Magno da Rocha et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.</rights><rights>COPYRIGHT 2023 Public Library of Science</rights><rights>2023 Magno da Rocha et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2023 Magno da Rocha et al 2023 Magno da Rocha et al</rights><rights>2023 Magno da Rocha et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. 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William</contributor><creatorcontrib>Magno da Rocha, Vinícius</creatorcontrib><creatorcontrib>Lima, Carla Ormundo Gonçalves Ximenes</creatorcontrib><creatorcontrib>Ferreira, Eliane de Oliveira</creatorcontrib><creatorcontrib>de Farias, Gabriel Corrêa</creatorcontrib><creatorcontrib>Nogueira, Fábio César Sousa</creatorcontrib><creatorcontrib>Martha Antunes, Luis Caetano</creatorcontrib><creatorcontrib>Cassiano, Keila Mara</creatorcontrib><creatorcontrib>Fiorelli, Rossano Kepler Alvim</creatorcontrib><title>Colonization of intervertebral discs by Cutibacterium acnes in patients with low back pain: Protocol for an analytical study with microbiological, phenotypic, genotypic, and multiomic techniques</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Lumbar disc degeneration (LDD) and low back pain (LBP) are two conditions that are closely related. Several studies have shown Cutibacterium acnes colonization of degenerated discs, but whether and how these finding correlates with LBP is unknown. A prospective study was planned to identify molecules present in lumbar intervertebral discs (LLIVD) colonized by C. acnes in patients with LDD and LBP and correlate them with their clinical, radiological, and demographic profiles. The clinical manifestations, risk factors, and demographic characteristics of participants undergoing surgical microdiscectomy will be tracked. Samples will be isolated and pathogens found in LLIVD will be characterized phenotypically and genotypically. Whole genome sequencing (WGS) of isolated species will be used to phylotype and detect genes associated with virulence, resistance, and oxidative stress. Multiomic analyses of LLIVD colonized and non-colonized will be carried out to explain not only the pathogen's role in LDD, but also its involvement in the pathophysiology of LBP. 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William</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Colonization of intervertebral discs by Cutibacterium acnes in patients with low back pain: Protocol for an analytical study with microbiological, phenotypic, genotypic, and multiomic techniques</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2023-02-27</date><risdate>2023</risdate><volume>18</volume><issue>2</issue><spage>e0271773</spage><epage>e0271773</epage><pages>e0271773-e0271773</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Lumbar disc degeneration (LDD) and low back pain (LBP) are two conditions that are closely related. Several studies have shown Cutibacterium acnes colonization of degenerated discs, but whether and how these finding correlates with LBP is unknown. A prospective study was planned to identify molecules present in lumbar intervertebral discs (LLIVD) colonized by C. acnes in patients with LDD and LBP and correlate them with their clinical, radiological, and demographic profiles. The clinical manifestations, risk factors, and demographic characteristics of participants undergoing surgical microdiscectomy will be tracked. Samples will be isolated and pathogens found in LLIVD will be characterized phenotypically and genotypically. Whole genome sequencing (WGS) of isolated species will be used to phylotype and detect genes associated with virulence, resistance, and oxidative stress. Multiomic analyses of LLIVD colonized and non-colonized will be carried out to explain not only the pathogen's role in LDD, but also its involvement in the pathophysiology of LBP. This study was approved by the Institutional Review Board (CAAE 50077521.0.0000.5258). All patients who agree to participate in the study will sign an informed consent form. Regardless of the study's findings, the results will be published in a peer-reviewed medical journal. Trials registration number NCT05090553; pre-results.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>36848344</pmid><doi>10.1371/journal.pone.0271773</doi><tpages>e0271773</tpages><orcidid>https://orcid.org/0000-0001-5507-7142</orcidid><orcidid>https://orcid.org/0000-0003-1686-0788</orcidid><orcidid>https://orcid.org/0000-0002-9977-6460</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2023-02, Vol.18 (2), p.e0271773-e0271773 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_2780394383 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Public Library of Science (PLoS) Journals Open Access; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Analysis Antibiotics Back pain Back surgery Biofilms Biology and Life Sciences Care and treatment Causes of Clinical trials Colonization Computer and Information Sciences Control Cutibacterium acnes Degeneration Demographics Demography Diagnosis Engineering and Technology Gene sequencing Genomes Hernia Humans Hypotheses Identification and classification Informed consent Intervertebral Disc Intervertebral Disc Degeneration - genetics Intervertebral discs Intervertebral disk Intervertebral disk displacement Laboratories Low back pain Low Back Pain - genetics Medicine and Health Sciences Multiomics Oxidation resistance Oxidative stress Pain Pathogenic microorganisms Pathogens Patients Physical Sciences Propionibacterium acnes - genetics Prospective Studies Prostheses Proteins Research and Analysis Methods Review boards Risk factors Study Protocol Virulence Virulence (Microbiology) Whole genome sequencing |
| title | Colonization of intervertebral discs by Cutibacterium acnes in patients with low back pain: Protocol for an analytical study with microbiological, phenotypic, genotypic, and multiomic techniques |
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