Multisite imaging of neural activity using a genetically encoded calcium sensor in the honey bee
Understanding of the neural bases for complex behaviors in Hymenoptera insect species has been limited by a lack of tools that allow measuring neuronal activity simultaneously in different brain regions. Here, we developed the first pan-neuronal genetic driver in a Hymenopteran model organism, the h...
Gespeichert in:
| Veröffentlicht in: | PLoS biology 2023-01, Vol.21 (1), p.e3001984-e3001984 |
|---|---|
| Hauptverfasser: | , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | e3001984 |
|---|---|
| container_issue | 1 |
| container_start_page | e3001984 |
| container_title | PLoS biology |
| container_volume | 21 |
| creator | Carcaud, Julie Otte, Marianne Grünewald, Bernd Haase, Albrecht Sandoz, Jean-Christophe Beye, Martin |
| description | Understanding of the neural bases for complex behaviors in Hymenoptera insect species has been limited by a lack of tools that allow measuring neuronal activity simultaneously in different brain regions. Here, we developed the first pan-neuronal genetic driver in a Hymenopteran model organism, the honey bee, and expressed the calcium indicator GCaMP6f under the control of the honey bee synapsin promoter. We show that GCaMP6f is widely expressed in the honey bee brain, allowing to record neural activity from multiple brain regions. To assess the power of this tool, we focused on the olfactory system, recording simultaneous responses from the antennal lobe, and from the more poorly investigated lateral horn (LH) and mushroom body (MB) calyces. Neural responses to 16 distinct odorants demonstrate that odorant quality (chemical structure) and quantity are faithfully encoded in the honey bee antennal lobe. In contrast, odor coding in the LH departs from this simple physico-chemical coding, supporting the role of this structure in coding the biological value of odorants. We further demonstrate robust neural responses to several bee pheromone odorants, key drivers of social behavior, in the LH. Combined, these brain recordings represent the first use of a neurogenetic tool for recording large-scale neural activity in a eusocial insect and will be of utility in assessing the neural underpinnings of olfactory and other sensory modalities and of social behaviors and cognitive abilities. |
| doi_str_mv | 10.1371/journal.pbio.3001984 |
| format | Article |
| fullrecord | <record><control><sourceid>gale_plos_</sourceid><recordid>TN_cdi_plos_journals_2777430379</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A736385452</galeid><doaj_id>oai_doaj_org_article_c4e86531bb6f43d584b4bfae6229787c</doaj_id><sourcerecordid>A736385452</sourcerecordid><originalsourceid>FETCH-LOGICAL-c729t-cec17450370516b000e560a8cd79204c663b8bdc5b92d0693acc1fe250c5e2de3</originalsourceid><addsrcrecordid>eNqVk11v0zAUhiMEYmPwDxBY4oZdtPgzjm-QqglYpcIkvm6N45ykrlK7xElF_z3umk3rtAtQLpIcP-_rnNc5WfaS4ClhkrxbhaHzpp1uShemDGOiCv4oOyWCi4ksCvH4zvNJ9izGFcaUKlo8zU5YLolSVJ5mvz4Pbe-i6wG5tWmcb1CokYehMy0ytndb1-_QEPcLBjXgoXfWtO0OgbehggqlN-uGNYrgY-iQ86hfAloGDztUAjzPntSmjfBivJ9lPz5--H5xOVlcfZpfzBYTK6nqJxYskVxgJrEgeYkxBpFjU9hKKoq5zXNWFmVlRalohXPFjLWkBiqwFUArYGfZ64Pvpg1Rj-FETaWUnCVblYj5gaiCWelNl_rtdjoYp68LoWu06VJ3LWjLocgFI2WZ15xVouAlL2sDeQpQFtImr_fjbkO5hsqC71NgR6bHK94tdRO2WikiGebJ4PxgsLwnu5wt9L6GOVWCqHxLEvt23KwLvweIvV67aKFtjYcwXPdIGGNEiYS-uYc-nMRINSY163wd0jfavameSZazIv03NFHTB6h0VbB2Np1v7VL9SHB-JEhMD3_6xgwx6vm3r__Bfvl39urnMcsPrO1CjB3Ut-ESrPdzcxOI3s-NHucmyV7dPc9b0c2gsL8guxG5</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2777430379</pqid></control><display><type>article</type><title>Multisite imaging of neural activity using a genetically encoded calcium sensor in the honey bee</title><source>MEDLINE</source><source>DOAJ Directory of Open Access Journals</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>Public Library of Science (PLoS)</source><source>PubMed Central</source><creator>Carcaud, Julie ; Otte, Marianne ; Grünewald, Bernd ; Haase, Albrecht ; Sandoz, Jean-Christophe ; Beye, Martin</creator><contributor>Chittka, Lars</contributor><creatorcontrib>Carcaud, Julie ; Otte, Marianne ; Grünewald, Bernd ; Haase, Albrecht ; Sandoz, Jean-Christophe ; Beye, Martin ; Chittka, Lars</creatorcontrib><description>Understanding of the neural bases for complex behaviors in Hymenoptera insect species has been limited by a lack of tools that allow measuring neuronal activity simultaneously in different brain regions. Here, we developed the first pan-neuronal genetic driver in a Hymenopteran model organism, the honey bee, and expressed the calcium indicator GCaMP6f under the control of the honey bee synapsin promoter. We show that GCaMP6f is widely expressed in the honey bee brain, allowing to record neural activity from multiple brain regions. To assess the power of this tool, we focused on the olfactory system, recording simultaneous responses from the antennal lobe, and from the more poorly investigated lateral horn (LH) and mushroom body (MB) calyces. Neural responses to 16 distinct odorants demonstrate that odorant quality (chemical structure) and quantity are faithfully encoded in the honey bee antennal lobe. In contrast, odor coding in the LH departs from this simple physico-chemical coding, supporting the role of this structure in coding the biological value of odorants. We further demonstrate robust neural responses to several bee pheromone odorants, key drivers of social behavior, in the LH. Combined, these brain recordings represent the first use of a neurogenetic tool for recording large-scale neural activity in a eusocial insect and will be of utility in assessing the neural underpinnings of olfactory and other sensory modalities and of social behaviors and cognitive abilities.</description><identifier>ISSN: 1545-7885</identifier><identifier>ISSN: 1544-9173</identifier><identifier>EISSN: 1545-7885</identifier><identifier>DOI: 10.1371/journal.pbio.3001984</identifier><identifier>PMID: 36719927</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Animal cognition ; Animals ; Antennal lobe ; Apis mellifera ; Aroma compounds ; Bees ; Bees - genetics ; Behavior ; Biochemistry, Molecular Biology ; Biology and Life Sciences ; Biosensors ; Brain ; Brain - physiology ; Brain research ; Calcium ; Coding ; Cognitive ability ; Eusociality ; Gene expression ; Genetic aspects ; Genetic code ; Genetic engineering ; Genomes ; Genomics ; Honey ; Honeybee ; Insects ; Life Sciences ; Luteinizing hormone ; Methods and Resources ; Mushroom bodies ; Neural circuitry ; Neural coding ; Neurobiology ; Neuroimaging ; Neurons ; Neurons and Cognition ; Odorants ; Odors ; Olfactory system ; Pheromones - genetics ; Physical Sciences ; Physiological aspects ; Proteins ; Recording ; Research and Analysis Methods ; Sensors ; Sensory evaluation ; Smell - genetics ; Social behavior ; Social Sciences ; Synapsin</subject><ispartof>PLoS biology, 2023-01, Vol.21 (1), p.e3001984-e3001984</ispartof><rights>Copyright: © 2023 Carcaud et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.</rights><rights>COPYRIGHT 2023 Public Library of Science</rights><rights>2023 Carcaud et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><rights>2023 Carcaud et al 2023 Carcaud et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c729t-cec17450370516b000e560a8cd79204c663b8bdc5b92d0693acc1fe250c5e2de3</citedby><cites>FETCH-LOGICAL-c729t-cec17450370516b000e560a8cd79204c663b8bdc5b92d0693acc1fe250c5e2de3</cites><orcidid>0000-0003-3672-9963</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC9917304/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC9917304/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,724,777,781,861,882,2096,2915,23847,27905,27906,53772,53774,79349,79350</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/36719927$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://hal.science/hal-04295196$$DView record in HAL$$Hfree_for_read</backlink></links><search><contributor>Chittka, Lars</contributor><creatorcontrib>Carcaud, Julie</creatorcontrib><creatorcontrib>Otte, Marianne</creatorcontrib><creatorcontrib>Grünewald, Bernd</creatorcontrib><creatorcontrib>Haase, Albrecht</creatorcontrib><creatorcontrib>Sandoz, Jean-Christophe</creatorcontrib><creatorcontrib>Beye, Martin</creatorcontrib><title>Multisite imaging of neural activity using a genetically encoded calcium sensor in the honey bee</title><title>PLoS biology</title><addtitle>PLoS Biol</addtitle><description>Understanding of the neural bases for complex behaviors in Hymenoptera insect species has been limited by a lack of tools that allow measuring neuronal activity simultaneously in different brain regions. Here, we developed the first pan-neuronal genetic driver in a Hymenopteran model organism, the honey bee, and expressed the calcium indicator GCaMP6f under the control of the honey bee synapsin promoter. We show that GCaMP6f is widely expressed in the honey bee brain, allowing to record neural activity from multiple brain regions. To assess the power of this tool, we focused on the olfactory system, recording simultaneous responses from the antennal lobe, and from the more poorly investigated lateral horn (LH) and mushroom body (MB) calyces. Neural responses to 16 distinct odorants demonstrate that odorant quality (chemical structure) and quantity are faithfully encoded in the honey bee antennal lobe. In contrast, odor coding in the LH departs from this simple physico-chemical coding, supporting the role of this structure in coding the biological value of odorants. We further demonstrate robust neural responses to several bee pheromone odorants, key drivers of social behavior, in the LH. Combined, these brain recordings represent the first use of a neurogenetic tool for recording large-scale neural activity in a eusocial insect and will be of utility in assessing the neural underpinnings of olfactory and other sensory modalities and of social behaviors and cognitive abilities.</description><subject>Animal cognition</subject><subject>Animals</subject><subject>Antennal lobe</subject><subject>Apis mellifera</subject><subject>Aroma compounds</subject><subject>Bees</subject><subject>Bees - genetics</subject><subject>Behavior</subject><subject>Biochemistry, Molecular Biology</subject><subject>Biology and Life Sciences</subject><subject>Biosensors</subject><subject>Brain</subject><subject>Brain - physiology</subject><subject>Brain research</subject><subject>Calcium</subject><subject>Coding</subject><subject>Cognitive ability</subject><subject>Eusociality</subject><subject>Gene expression</subject><subject>Genetic aspects</subject><subject>Genetic code</subject><subject>Genetic engineering</subject><subject>Genomes</subject><subject>Genomics</subject><subject>Honey</subject><subject>Honeybee</subject><subject>Insects</subject><subject>Life Sciences</subject><subject>Luteinizing hormone</subject><subject>Methods and Resources</subject><subject>Mushroom bodies</subject><subject>Neural circuitry</subject><subject>Neural coding</subject><subject>Neurobiology</subject><subject>Neuroimaging</subject><subject>Neurons</subject><subject>Neurons and Cognition</subject><subject>Odorants</subject><subject>Odors</subject><subject>Olfactory system</subject><subject>Pheromones - genetics</subject><subject>Physical Sciences</subject><subject>Physiological aspects</subject><subject>Proteins</subject><subject>Recording</subject><subject>Research and Analysis Methods</subject><subject>Sensors</subject><subject>Sensory evaluation</subject><subject>Smell - genetics</subject><subject>Social behavior</subject><subject>Social Sciences</subject><subject>Synapsin</subject><issn>1545-7885</issn><issn>1544-9173</issn><issn>1545-7885</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>DOA</sourceid><recordid>eNqVk11v0zAUhiMEYmPwDxBY4oZdtPgzjm-QqglYpcIkvm6N45ykrlK7xElF_z3umk3rtAtQLpIcP-_rnNc5WfaS4ClhkrxbhaHzpp1uShemDGOiCv4oOyWCi4ksCvH4zvNJ9izGFcaUKlo8zU5YLolSVJ5mvz4Pbe-i6wG5tWmcb1CokYehMy0ytndb1-_QEPcLBjXgoXfWtO0OgbehggqlN-uGNYrgY-iQ86hfAloGDztUAjzPntSmjfBivJ9lPz5--H5xOVlcfZpfzBYTK6nqJxYskVxgJrEgeYkxBpFjU9hKKoq5zXNWFmVlRalohXPFjLWkBiqwFUArYGfZ64Pvpg1Rj-FETaWUnCVblYj5gaiCWelNl_rtdjoYp68LoWu06VJ3LWjLocgFI2WZ15xVouAlL2sDeQpQFtImr_fjbkO5hsqC71NgR6bHK94tdRO2WikiGebJ4PxgsLwnu5wt9L6GOVWCqHxLEvt23KwLvweIvV67aKFtjYcwXPdIGGNEiYS-uYc-nMRINSY163wd0jfavameSZazIv03NFHTB6h0VbB2Np1v7VL9SHB-JEhMD3_6xgwx6vm3r__Bfvl39urnMcsPrO1CjB3Ut-ESrPdzcxOI3s-NHucmyV7dPc9b0c2gsL8guxG5</recordid><startdate>20230101</startdate><enddate>20230101</enddate><creator>Carcaud, Julie</creator><creator>Otte, Marianne</creator><creator>Grünewald, Bernd</creator><creator>Haase, Albrecht</creator><creator>Sandoz, Jean-Christophe</creator><creator>Beye, Martin</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISN</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TK</scope><scope>7TM</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PATMY</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>1XC</scope><scope>VOOES</scope><scope>5PM</scope><scope>DOA</scope><scope>CZG</scope><orcidid>https://orcid.org/0000-0003-3672-9963</orcidid></search><sort><creationdate>20230101</creationdate><title>Multisite imaging of neural activity using a genetically encoded calcium sensor in the honey bee</title><author>Carcaud, Julie ; Otte, Marianne ; Grünewald, Bernd ; Haase, Albrecht ; Sandoz, Jean-Christophe ; Beye, Martin</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c729t-cec17450370516b000e560a8cd79204c663b8bdc5b92d0693acc1fe250c5e2de3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Animal cognition</topic><topic>Animals</topic><topic>Antennal lobe</topic><topic>Apis mellifera</topic><topic>Aroma compounds</topic><topic>Bees</topic><topic>Bees - genetics</topic><topic>Behavior</topic><topic>Biochemistry, Molecular Biology</topic><topic>Biology and Life Sciences</topic><topic>Biosensors</topic><topic>Brain</topic><topic>Brain - physiology</topic><topic>Brain research</topic><topic>Calcium</topic><topic>Coding</topic><topic>Cognitive ability</topic><topic>Eusociality</topic><topic>Gene expression</topic><topic>Genetic aspects</topic><topic>Genetic code</topic><topic>Genetic engineering</topic><topic>Genomes</topic><topic>Genomics</topic><topic>Honey</topic><topic>Honeybee</topic><topic>Insects</topic><topic>Life Sciences</topic><topic>Luteinizing hormone</topic><topic>Methods and Resources</topic><topic>Mushroom bodies</topic><topic>Neural circuitry</topic><topic>Neural coding</topic><topic>Neurobiology</topic><topic>Neuroimaging</topic><topic>Neurons</topic><topic>Neurons and Cognition</topic><topic>Odorants</topic><topic>Odors</topic><topic>Olfactory system</topic><topic>Pheromones - genetics</topic><topic>Physical Sciences</topic><topic>Physiological aspects</topic><topic>Proteins</topic><topic>Recording</topic><topic>Research and Analysis Methods</topic><topic>Sensors</topic><topic>Sensory evaluation</topic><topic>Smell - genetics</topic><topic>Social behavior</topic><topic>Social Sciences</topic><topic>Synapsin</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Carcaud, Julie</creatorcontrib><creatorcontrib>Otte, Marianne</creatorcontrib><creatorcontrib>Grünewald, Bernd</creatorcontrib><creatorcontrib>Haase, Albrecht</creatorcontrib><creatorcontrib>Sandoz, Jean-Christophe</creatorcontrib><creatorcontrib>Beye, Martin</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: Opposing Viewpoints</collection><collection>Gale In Context: Canada</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Environmental Science Collection</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>Hyper Article en Ligne (HAL)</collection><collection>Hyper Article en Ligne (HAL) (Open Access)</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><collection>PLoS Biology</collection><jtitle>PLoS biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Carcaud, Julie</au><au>Otte, Marianne</au><au>Grünewald, Bernd</au><au>Haase, Albrecht</au><au>Sandoz, Jean-Christophe</au><au>Beye, Martin</au><au>Chittka, Lars</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Multisite imaging of neural activity using a genetically encoded calcium sensor in the honey bee</atitle><jtitle>PLoS biology</jtitle><addtitle>PLoS Biol</addtitle><date>2023-01-01</date><risdate>2023</risdate><volume>21</volume><issue>1</issue><spage>e3001984</spage><epage>e3001984</epage><pages>e3001984-e3001984</pages><issn>1545-7885</issn><issn>1544-9173</issn><eissn>1545-7885</eissn><abstract>Understanding of the neural bases for complex behaviors in Hymenoptera insect species has been limited by a lack of tools that allow measuring neuronal activity simultaneously in different brain regions. Here, we developed the first pan-neuronal genetic driver in a Hymenopteran model organism, the honey bee, and expressed the calcium indicator GCaMP6f under the control of the honey bee synapsin promoter. We show that GCaMP6f is widely expressed in the honey bee brain, allowing to record neural activity from multiple brain regions. To assess the power of this tool, we focused on the olfactory system, recording simultaneous responses from the antennal lobe, and from the more poorly investigated lateral horn (LH) and mushroom body (MB) calyces. Neural responses to 16 distinct odorants demonstrate that odorant quality (chemical structure) and quantity are faithfully encoded in the honey bee antennal lobe. In contrast, odor coding in the LH departs from this simple physico-chemical coding, supporting the role of this structure in coding the biological value of odorants. We further demonstrate robust neural responses to several bee pheromone odorants, key drivers of social behavior, in the LH. Combined, these brain recordings represent the first use of a neurogenetic tool for recording large-scale neural activity in a eusocial insect and will be of utility in assessing the neural underpinnings of olfactory and other sensory modalities and of social behaviors and cognitive abilities.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>36719927</pmid><doi>10.1371/journal.pbio.3001984</doi><orcidid>https://orcid.org/0000-0003-3672-9963</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1545-7885 |
| ispartof | PLoS biology, 2023-01, Vol.21 (1), p.e3001984-e3001984 |
| issn | 1545-7885 1544-9173 1545-7885 |
| language | eng |
| recordid | cdi_plos_journals_2777430379 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Public Library of Science (PLoS); PubMed Central |
| subjects | Animal cognition Animals Antennal lobe Apis mellifera Aroma compounds Bees Bees - genetics Behavior Biochemistry, Molecular Biology Biology and Life Sciences Biosensors Brain Brain - physiology Brain research Calcium Coding Cognitive ability Eusociality Gene expression Genetic aspects Genetic code Genetic engineering Genomes Genomics Honey Honeybee Insects Life Sciences Luteinizing hormone Methods and Resources Mushroom bodies Neural circuitry Neural coding Neurobiology Neuroimaging Neurons Neurons and Cognition Odorants Odors Olfactory system Pheromones - genetics Physical Sciences Physiological aspects Proteins Recording Research and Analysis Methods Sensors Sensory evaluation Smell - genetics Social behavior Social Sciences Synapsin |
| title | Multisite imaging of neural activity using a genetically encoded calcium sensor in the honey bee |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-19T16%3A37%3A31IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Multisite%20imaging%20of%20neural%20activity%20using%20a%20genetically%20encoded%20calcium%20sensor%20in%20the%20honey%20bee&rft.jtitle=PLoS%20biology&rft.au=Carcaud,%20Julie&rft.date=2023-01-01&rft.volume=21&rft.issue=1&rft.spage=e3001984&rft.epage=e3001984&rft.pages=e3001984-e3001984&rft.issn=1545-7885&rft.eissn=1545-7885&rft_id=info:doi/10.1371/journal.pbio.3001984&rft_dat=%3Cgale_plos_%3EA736385452%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2777430379&rft_id=info:pmid/36719927&rft_galeid=A736385452&rft_doaj_id=oai_doaj_org_article_c4e86531bb6f43d584b4bfae6229787c&rfr_iscdi=true |