Cytokine response in asymptomatic and symptomatic Plasmodium falciparum infections in children in a rural area of south-eastern Gabon
Plasmodium falciparum is a parasite that causes asymptomatic or symptomatic malaria infections in humans depending on various factors. These infections are also a major cause of anemia in intertropical countries such as Gabon. Past studies have clearly demonstrated that inflammatory markers such as...
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| Veröffentlicht in: | PloS one 2023-02, Vol.18 (2), p.e0280818-e0280818 |
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| creator | Oyegue-Liabagui, Sandrine Lydie Mbani Mpega Ntigui, Chérone Nancy Ada Mengome, Mérédith Flore Kouna, Lady Charlene Tsafack Tegomo, Nathalie Pernelle Longo Pendy, Neil Michel Lekana-Douki, Jean-Bernard |
| description | Plasmodium falciparum is a parasite that causes asymptomatic or symptomatic malaria infections in humans depending on various factors. These infections are also a major cause of anemia in intertropical countries such as Gabon. Past studies have clearly demonstrated that inflammatory markers such as cytokines play a key role in the pathogenesis of malaria disease. However, the clinical manifestations of severe malaria vary according to the level of transmission and more information is needed to gain a better understanding of the factors involved. As such, the objective of this study was to investigate the circulating levels of nine cytokines in asymptomatic and symptomatic P. falciparum infections in Gabonese children and their roles in the pathogenesis of anemia. Blood samples were collected from 241 children aged 3 to 180 months in Lastourville, south-eastern Gabon. Diagnosis of P. falciparum infection was performed using Rapid Diagnosis Tests, microscopy and nested PCR. Levels in the plasma of the Th1 (IFN-γ, TNF-α, IL-6 and IL-12p70), Th17 (IL-17A and IL-22) and Th2 (IL-10, IL-4 and IL-13) cytokines were measured by ELISA. Data showed that IL-6, IFN-γ, IL-12p70, IL-10, and IL-13 levels were significantly higher in children with symptomatic P. falciparum infection compared to uninfected children. IL-10 levels were significantly higher in symptomatic children than in asymptomatic children, who had moderately increased levels compared to uninfected controls. Moreover, only IL-10 and IL-6 levels were significantly higher in children with severe malarial anemia compared to children with uncomplicated malaria who had significantly lower IL-10 levels than children with moderate malarial anemia. These data indicate that the progression of P. falciparum infection towards an advanced stage in children is accompanied by a significant increase in type Th1 and/or Th2 cytokines. These inflammatory mediators could serve as potential predictors of anemia for malaria patients. |
| doi_str_mv | 10.1371/journal.pone.0280818 |
| format | Article |
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These infections are also a major cause of anemia in intertropical countries such as Gabon. Past studies have clearly demonstrated that inflammatory markers such as cytokines play a key role in the pathogenesis of malaria disease. However, the clinical manifestations of severe malaria vary according to the level of transmission and more information is needed to gain a better understanding of the factors involved. As such, the objective of this study was to investigate the circulating levels of nine cytokines in asymptomatic and symptomatic P. falciparum infections in Gabonese children and their roles in the pathogenesis of anemia. Blood samples were collected from 241 children aged 3 to 180 months in Lastourville, south-eastern Gabon. Diagnosis of P. falciparum infection was performed using Rapid Diagnosis Tests, microscopy and nested PCR. Levels in the plasma of the Th1 (IFN-γ, TNF-α, IL-6 and IL-12p70), Th17 (IL-17A and IL-22) and Th2 (IL-10, IL-4 and IL-13) cytokines were measured by ELISA. Data showed that IL-6, IFN-γ, IL-12p70, IL-10, and IL-13 levels were significantly higher in children with symptomatic P. falciparum infection compared to uninfected children. IL-10 levels were significantly higher in symptomatic children than in asymptomatic children, who had moderately increased levels compared to uninfected controls. Moreover, only IL-10 and IL-6 levels were significantly higher in children with severe malarial anemia compared to children with uncomplicated malaria who had significantly lower IL-10 levels than children with moderate malarial anemia. These data indicate that the progression of P. falciparum infection towards an advanced stage in children is accompanied by a significant increase in type Th1 and/or Th2 cytokines. These inflammatory mediators could serve as potential predictors of anemia for malaria patients.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0280818</identifier><identifier>PMID: 36787308</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Anemia ; Anemia - etiology ; Asymptomatic ; Bacterial infections ; Biology and Life Sciences ; Blood ; Child ; Children ; Complications and side effects ; Cytokines ; Development and progression ; Diagnosis ; Diagnostic tests ; Disease ; Diseases ; Enzyme-linked immunosorbent assay ; Females ; Gabon ; Health aspects ; Helper cells ; Hemoglobin ; Humans ; Infection ; Infections ; Inflammation ; Interleukin 10 ; Interleukin 12 ; Interleukin 13 ; Interleukin 22 ; Interleukin 4 ; Interleukin 6 ; Lymphocytes T ; Malaria ; Malaria - complications ; Malaria, Falciparum - parasitology ; Medicine and Health Sciences ; Microscopy ; Parasites ; Pathogenesis ; Pediatrics ; Plasmodium falciparum ; Polymerase chain reaction ; Research and Analysis Methods ; Rural areas ; Sociodemographics ; Statistical analysis ; Tumor necrosis factor-α ; Values ; Vector-borne diseases ; γ-Interferon</subject><ispartof>PloS one, 2023-02, Vol.18 (2), p.e0280818-e0280818</ispartof><rights>Copyright: © 2023 Oyegue-Liabagui et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.</rights><rights>COPYRIGHT 2023 Public Library of Science</rights><rights>2023 Oyegue-Liabagui et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2023 Oyegue-Liabagui et al 2023 Oyegue-Liabagui et al</rights><rights>2023 Oyegue-Liabagui et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. 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These infections are also a major cause of anemia in intertropical countries such as Gabon. Past studies have clearly demonstrated that inflammatory markers such as cytokines play a key role in the pathogenesis of malaria disease. However, the clinical manifestations of severe malaria vary according to the level of transmission and more information is needed to gain a better understanding of the factors involved. As such, the objective of this study was to investigate the circulating levels of nine cytokines in asymptomatic and symptomatic P. falciparum infections in Gabonese children and their roles in the pathogenesis of anemia. Blood samples were collected from 241 children aged 3 to 180 months in Lastourville, south-eastern Gabon. Diagnosis of P. falciparum infection was performed using Rapid Diagnosis Tests, microscopy and nested PCR. 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These inflammatory mediators could serve as potential predictors of anemia for malaria patients.</description><subject>Anemia</subject><subject>Anemia - etiology</subject><subject>Asymptomatic</subject><subject>Bacterial infections</subject><subject>Biology and Life Sciences</subject><subject>Blood</subject><subject>Child</subject><subject>Children</subject><subject>Complications and side effects</subject><subject>Cytokines</subject><subject>Development and progression</subject><subject>Diagnosis</subject><subject>Diagnostic tests</subject><subject>Disease</subject><subject>Diseases</subject><subject>Enzyme-linked immunosorbent assay</subject><subject>Females</subject><subject>Gabon</subject><subject>Health aspects</subject><subject>Helper cells</subject><subject>Hemoglobin</subject><subject>Humans</subject><subject>Infection</subject><subject>Infections</subject><subject>Inflammation</subject><subject>Interleukin 10</subject><subject>Interleukin 12</subject><subject>Interleukin 13</subject><subject>Interleukin 22</subject><subject>Interleukin 4</subject><subject>Interleukin 6</subject><subject>Lymphocytes T</subject><subject>Malaria</subject><subject>Malaria - complications</subject><subject>Malaria, Falciparum - parasitology</subject><subject>Medicine and Health Sciences</subject><subject>Microscopy</subject><subject>Parasites</subject><subject>Pathogenesis</subject><subject>Pediatrics</subject><subject>Plasmodium falciparum</subject><subject>Polymerase chain reaction</subject><subject>Research and Analysis Methods</subject><subject>Rural areas</subject><subject>Sociodemographics</subject><subject>Statistical analysis</subject><subject>Tumor necrosis factor-α</subject><subject>Values</subject><subject>Vector-borne 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Gabon</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2023-02-14</date><risdate>2023</risdate><volume>18</volume><issue>2</issue><spage>e0280818</spage><epage>e0280818</epage><pages>e0280818-e0280818</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Plasmodium falciparum is a parasite that causes asymptomatic or symptomatic malaria infections in humans depending on various factors. These infections are also a major cause of anemia in intertropical countries such as Gabon. Past studies have clearly demonstrated that inflammatory markers such as cytokines play a key role in the pathogenesis of malaria disease. However, the clinical manifestations of severe malaria vary according to the level of transmission and more information is needed to gain a better understanding of the factors involved. As such, the objective of this study was to investigate the circulating levels of nine cytokines in asymptomatic and symptomatic P. falciparum infections in Gabonese children and their roles in the pathogenesis of anemia. Blood samples were collected from 241 children aged 3 to 180 months in Lastourville, south-eastern Gabon. Diagnosis of P. falciparum infection was performed using Rapid Diagnosis Tests, microscopy and nested PCR. Levels in the plasma of the Th1 (IFN-γ, TNF-α, IL-6 and IL-12p70), Th17 (IL-17A and IL-22) and Th2 (IL-10, IL-4 and IL-13) cytokines were measured by ELISA. Data showed that IL-6, IFN-γ, IL-12p70, IL-10, and IL-13 levels were significantly higher in children with symptomatic P. falciparum infection compared to uninfected children. IL-10 levels were significantly higher in symptomatic children than in asymptomatic children, who had moderately increased levels compared to uninfected controls. Moreover, only IL-10 and IL-6 levels were significantly higher in children with severe malarial anemia compared to children with uncomplicated malaria who had significantly lower IL-10 levels than children with moderate malarial anemia. These data indicate that the progression of P. falciparum infection towards an advanced stage in children is accompanied by a significant increase in type Th1 and/or Th2 cytokines. These inflammatory mediators could serve as potential predictors of anemia for malaria patients.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>36787308</pmid><doi>10.1371/journal.pone.0280818</doi><tpages>e0280818</tpages><orcidid>https://orcid.org/0000-0002-1689-2459</orcidid><orcidid>https://orcid.org/0000-0001-5649-8061</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2023-02, Vol.18 (2), p.e0280818-e0280818 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_2776415965 |
| source | Public Library of Science (PLoS) Journals Open Access; MEDLINE; DOAJ Directory of Open Access Journals; EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Anemia Anemia - etiology Asymptomatic Bacterial infections Biology and Life Sciences Blood Child Children Complications and side effects Cytokines Development and progression Diagnosis Diagnostic tests Disease Diseases Enzyme-linked immunosorbent assay Females Gabon Health aspects Helper cells Hemoglobin Humans Infection Infections Inflammation Interleukin 10 Interleukin 12 Interleukin 13 Interleukin 22 Interleukin 4 Interleukin 6 Lymphocytes T Malaria Malaria - complications Malaria, Falciparum - parasitology Medicine and Health Sciences Microscopy Parasites Pathogenesis Pediatrics Plasmodium falciparum Polymerase chain reaction Research and Analysis Methods Rural areas Sociodemographics Statistical analysis Tumor necrosis factor-α Values Vector-borne diseases γ-Interferon |
| title | Cytokine response in asymptomatic and symptomatic Plasmodium falciparum infections in children in a rural area of south-eastern Gabon |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-05T20%3A33%3A39IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Cytokine%20response%20in%20asymptomatic%20and%20symptomatic%20Plasmodium%20falciparum%20infections%20in%20children%20in%20a%20rural%20area%20of%20south-eastern%20Gabon&rft.jtitle=PloS%20one&rft.au=Oyegue-Liabagui,%20Sandrine%20Lydie&rft.date=2023-02-14&rft.volume=18&rft.issue=2&rft.spage=e0280818&rft.epage=e0280818&rft.pages=e0280818-e0280818&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0280818&rft_dat=%3Cgale_plos_%3EA737156082%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2776415965&rft_id=info:pmid/36787308&rft_galeid=A737156082&rft_doaj_id=oai_doaj_org_article_3f42a737f1b948f29f59de43f21f46fd&rfr_iscdi=true |