Rapid and sensitive point-of-care detection of Leptospira by RPA-CRISPR/Cas12a targeting lipL32

One of the key barriers preventing rapid diagnosis of leptospirosis is the lack of available sensitive point-of-care testing. This study aimed to develop and validate a clustered regularly-interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein 12a (CRISPR/Cas12a) platform combined w...

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Veröffentlicht in:	PLoS neglected tropical diseases 2022-01, Vol.16 (1), p.e0010112-e0010112
Hauptverfasser:	Jirawannaporn, Sirawit, Limothai, Umaporn, Tachaboon, Sasipha, Dinhuzen, Janejira, Kiatamornrak, Patcharakorn, Chaisuriyong, Watchadaporn, Bhumitrakul, Jom, Mayuramart, Oraphan, Payungporn, Sunchai, Srisawat, Nattachai
Format:	Artikel
Sprache:	eng
Schlagworte:	Agglutination tests Amplification Assaying Bacterial Outer Membrane Proteins - genetics Biology and Life Sciences Care and treatment Cells CRISPR CRISPR-Cas Systems - genetics Cross-reactivity Deoxyribonucleic acid Detection Diagnosis DNA Enzymes Female Fever Fluorescence Gene amplification Gene editing Health aspects Humans Infectious diseases Laboratories Leptospira Leptospira - genetics Leptospira - isolation & purification Leptospirosis Leptospirosis - diagnosis Limit of Detection Lipoproteins - genetics Male Mass Screening - methods Medicine and Health Sciences Middle Aged Molecular Diagnostic Techniques - methods Nucleic Acid Amplification Techniques - methods Pathogens Point-of-Care Testing Polymerase chain reaction Real-Time Polymerase Chain Reaction Recombinase Research and Analysis Methods Risk factors Samples Sensitivity Sensitivity analysis Sensitivity and Specificity Specificity Tropical diseases
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