A large-scale genetic screen identifies genes essential for motility in Agrobacterium fabrum
The genetic and molecular basis of flagellar motility has been investigated for several decades, with innovative research strategies propelling advances at a steady pace. Furthermore, as the phenomenon is examined in diverse bacteria, new taxon-specific regulatory and structural features are being e...
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| Veröffentlicht in: | PloS one 2023-01, Vol.18 (1), p.e0279936-e0279936 |
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| creator | Calvopina-Chavez, Diana G Howarth, Robyn E Memmott, Audrey K Pech Gonzalez, Oscar H Hafen, Caleb B Jensen, Kyson T Benedict, Alex B Altman, Jessica D Burnside, Brittany S Childs, Justin S Dallon, Samuel W DeMarco, Alexa C Flindt, Kirsten C Grover, Sarah A Heninger, Elizabeth Iverson, Christina S Johnson, Abigail K Lopez, Jack B Meinzer, McKay A Moulder, Brook A Moulton, Rebecca I Russell, Hyrum S Scott, Tiana M Shiobara, Yuka Taylor, Mason D Tippets, Kathryn E Vainerere, Kayla M Von Wallwitz, Isabella C Wagley, Madison Wiley, Megumi S Young, Naomi J Griffitts, Joel S |
| description | The genetic and molecular basis of flagellar motility has been investigated for several decades, with innovative research strategies propelling advances at a steady pace. Furthermore, as the phenomenon is examined in diverse bacteria, new taxon-specific regulatory and structural features are being elucidated. Motility is also a straightforward bacterial phenotype that can allow undergraduate researchers to explore the palette of molecular genetic tools available to microbiologists. This study, driven primarily by undergraduate researchers, evaluated hundreds of flagellar motility mutants in the Gram-negative plant-associated bacterium Agrobacterium fabrum. The nearly saturating screen implicates a total of 37 genes in flagellar biosynthesis, including genes of previously unknown function. |
| doi_str_mv | 10.1371/journal.pone.0279936 |
| format | Article |
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Furthermore, as the phenomenon is examined in diverse bacteria, new taxon-specific regulatory and structural features are being elucidated. Motility is also a straightforward bacterial phenotype that can allow undergraduate researchers to explore the palette of molecular genetic tools available to microbiologists. This study, driven primarily by undergraduate researchers, evaluated hundreds of flagellar motility mutants in the Gram-negative plant-associated bacterium Agrobacterium fabrum. 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large-scale genetic screen identifies genes essential for motility in Agrobacterium fabrum</title><author>Calvopina-Chavez, Diana G ; Howarth, Robyn E ; Memmott, Audrey K ; Pech Gonzalez, Oscar H ; Hafen, Caleb B ; Jensen, Kyson T ; Benedict, Alex B ; Altman, Jessica D ; Burnside, Brittany S ; Childs, Justin S ; Dallon, Samuel W ; DeMarco, Alexa C ; Flindt, Kirsten C ; Grover, Sarah A ; Heninger, Elizabeth ; Iverson, Christina S ; Johnson, Abigail K ; Lopez, Jack B ; Meinzer, McKay A ; Moulder, Brook A ; Moulton, Rebecca I ; Russell, Hyrum S ; Scott, Tiana M ; Shiobara, Yuka ; Taylor, Mason D ; Tippets, Kathryn E ; Vainerere, Kayla M ; Von Wallwitz, Isabella C ; Wagley, Madison ; Wiley, Megumi S ; Young, Naomi J ; Griffitts, Joel 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W</au><au>DeMarco, Alexa C</au><au>Flindt, Kirsten C</au><au>Grover, Sarah A</au><au>Heninger, Elizabeth</au><au>Iverson, Christina S</au><au>Johnson, Abigail K</au><au>Lopez, Jack B</au><au>Meinzer, McKay A</au><au>Moulder, Brook A</au><au>Moulton, Rebecca I</au><au>Russell, Hyrum S</au><au>Scott, Tiana M</au><au>Shiobara, Yuka</au><au>Taylor, Mason D</au><au>Tippets, Kathryn E</au><au>Vainerere, Kayla M</au><au>Von Wallwitz, Isabella C</au><au>Wagley, Madison</au><au>Wiley, Megumi S</au><au>Young, Naomi J</au><au>Griffitts, Joel S</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A large-scale genetic screen identifies genes essential for motility in Agrobacterium fabrum</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2023-01-04</date><risdate>2023</risdate><volume>18</volume><issue>1</issue><spage>e0279936</spage><epage>e0279936</epage><pages>e0279936-e0279936</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>The genetic and molecular basis of flagellar motility has been investigated for several decades, with innovative research strategies propelling advances at a steady pace. Furthermore, as the phenomenon is examined in diverse bacteria, new taxon-specific regulatory and structural features are being elucidated. Motility is also a straightforward bacterial phenotype that can allow undergraduate researchers to explore the palette of molecular genetic tools available to microbiologists. This study, driven primarily by undergraduate researchers, evaluated hundreds of flagellar motility mutants in the Gram-negative plant-associated bacterium Agrobacterium fabrum. The nearly saturating screen implicates a total of 37 genes in flagellar biosynthesis, including genes of previously unknown function.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>36598925</pmid><doi>10.1371/journal.pone.0279936</doi><tpages>e0279936</tpages><orcidid>https://orcid.org/0000-0001-6314-8895</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2023-01, Vol.18 (1), p.e0279936-e0279936 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_2760801079 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; Free Full-Text Journals in Chemistry; Public Library of Science (PLoS) |
| subjects | Agrobacterium Agrobacterium - genetics Antibiotics Bacteria Bacterial Proteins - genetics Biology and Life Sciences Biosynthesis Control Dosage and administration E coli Flagella Flagella (Microbiology) Flagella - metabolism Gene expression Gene Expression Regulation, Bacterial Genes Genetic screening Glycerol Identification and classification Medicine and Health Sciences Methods Molecular genetics Motility Mutagenesis Neomycin Phenotypes Proteins Research and analysis methods Stabbings |
| title | A large-scale genetic screen identifies genes essential for motility in Agrobacterium fabrum |
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