Identification of SaCas9 orthologs containing a conserved serine residue that determines simple NNGG PAM recognition

Due to different nucleotide preferences at target sites, no single Cas9 is capable of editing all sequences. Thus, this highlights the need to establish a Cas9 repertoire covering all sequences for efficient genome editing. Cas9s with simple protospacer adjacent motif (PAM) requirements are particul...

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Veröffentlicht in:	PLoS biology 2022-11, Vol.20 (11), p.e3001897-e3001897
Hauptverfasser:	Wang, Shuai, Tao, Chen, Mao, Huilin, Hou, Linghui, Wang, Yao, Qi, Tao, Yang, Yuan, Ong, Sang-Ging, Hu, Shijun, Chai, Renjie, Wang, Yongming
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acids Analysis Biology and Life Sciences CRISPR Databases, Factual DNA sequencing Editing Efficiency Engineering and Technology Gene loci Gene sequencing Genome editing Genomes Genomics Methods Mutation Nucleotide sequencing Nucleotides Physical Sciences Recognition, Psychology Research and Analysis Methods Residues Serine Serine - genetics
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