Ternary regulation mechanism of Rhizoma drynariae total flavonoids on induced membrane formation and bone remodeling in Masquelet technique
Rhizoma drynariae total flavonoids (RDTF) are used to treat fractures. CD31hiEmcnhi vessels induced by PDGF-BB secreted by osteoclast precursors, together with osteoblasts and osteoclasts, constitute the ternary regulatory mechanism of bone tissue reconstruction. This study aimed to determine whethe...
Gespeichert in:
| Veröffentlicht in: | PloS one 2022-12, Vol.17 (12), p.e0278688-e0278688 |
|---|---|
| Hauptverfasser: | , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | e0278688 |
|---|---|
| container_issue | 12 |
| container_start_page | e0278688 |
| container_title | PloS one |
| container_volume | 17 |
| creator | Li, Ding Zhao, Dun Zeng, Zhikui Huang, Feng Jiang, Ziwei Xiong, Hao Guan, Tianan Fang, Bin Li, Yue |
| description | Rhizoma drynariae total flavonoids (RDTF) are used to treat fractures. CD31hiEmcnhi vessels induced by PDGF-BB secreted by osteoclast precursors, together with osteoblasts and osteoclasts, constitute the ternary regulatory mechanism of bone tissue reconstruction.
This study aimed to determine whether RDTF can promote bone tissue remodeling and induce membrane growth in the rat Masquelet model and to explore its molecular mechanism based on the ternary regulation theory.
Thirty-six SD rats were randomized to three groups: blank, induced membrane, and RDTF treatment (n = 12/group). The gross morphological characteristics of the new bone tissue were observed after 6 weeks. Sixty SD rats were also randomized to five groups: blank, induction membrane, low-dose RDTF, medium-dose RDTF, and high-dose RDTF (n = 12/group). After 4 weeks, immunohistochemistry and western blot were used to detect the expression of membrane tissue-related proteins. The mRNA expression of key factors of ternary regulation was analyzed by qRT-PCR.
RDTF positively affected angiogenesis and bone tissue reconstruction in the bone defect area. RDTF could upregulate the expression of key factors (PDGF-BB, CD31, and endomucin), VEGF, and HMGB1 mRNA and proteins in the ternary regulation pathway.
Although the expected CD31hiEmcnhi vessels in the induction membrane were not observed, this study confirmed that RDTF could promote the secretion of angiogenic factors in the induced membrane. The specific mechanisms still need to be further studied. |
| doi_str_mv | 10.1371/journal.pone.0278688 |
| format | Article |
| fullrecord | <record><control><sourceid>gale_plos_</sourceid><recordid>TN_cdi_plos_journals_2747243370</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A729093587</galeid><doaj_id>oai_doaj_org_article_252334ec872c4fa98b7e77561d7247be</doaj_id><sourcerecordid>A729093587</sourcerecordid><originalsourceid>FETCH-LOGICAL-c641t-32f3ee91fd9928235d60f30d94d0e6da54c59639846e72d2c72dd9e55a11f6e43</originalsourceid><addsrcrecordid>eNqNk11rFDEUhgdRbK3-A9EBQfRi13zMJDM3Qil-FCqFWr0N2eTMbpZMsiYzxfoX_NOedaelK72QgfnIPO97kjc5RfGckjnlkr5bxzEF7eebGGBOmGxE0zwoDmnL2Uwwwh_eeT8onuS8JqTmjRCPiwMuKskJaQ6L35eALum6TLAcvR5cDGUPZqWDy30Zu_Ji5X7FXpc2XSPnNJRDHLQvO6-vYojO5hIlLtjRgEVpv0g6QNnF1O_cdLDlAueIFfpowbuwRLz8ovOPETwM5YDlgsOPp8WjTvsMz6bnUfHt44fLk8-zs_NPpyfHZzMjKjrMOOs4QEs727asYby2gnSc2LayBITVdWXqVvC2qQRIZpnBm22hrjWlnYCKHxUvd74bH7OacsyKyUqyinNJkDjdETbqtdok12NEKmqn_g7EtFQ6Dc54UKxmnFdgGslM1em2WUiQshbUoplcAHq9n6qNix6sgTAk7fdM9_8Et1LLeKVayWrKJBq8mQxSxJTyoHqXDXiPOcdxmrcUlDJEX_2D3r-6iVpqXIALXcS6ZmuqjiVrScvrZlt2fg-Fl4XeGdzQzuH4nuDtngCZAX4OSz3mrE6_Xvw_e_59n319h12B9sMqRz9uT1feB6sdaFLMOUF3GzIlatszN2mobc-oqWdQ9uLuBt2KbpqE_wHtTxO7</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2747243370</pqid></control><display><type>article</type><title>Ternary regulation mechanism of Rhizoma drynariae total flavonoids on induced membrane formation and bone remodeling in Masquelet technique</title><source>MEDLINE</source><source>DOAJ Directory of Open Access Journals</source><source>EZB-FREE-00999 freely available EZB journals</source><source>PubMed Central</source><source>Free Full-Text Journals in Chemistry</source><source>Public Library of Science (PLoS)</source><creator>Li, Ding ; Zhao, Dun ; Zeng, Zhikui ; Huang, Feng ; Jiang, Ziwei ; Xiong, Hao ; Guan, Tianan ; Fang, Bin ; Li, Yue</creator><creatorcontrib>Li, Ding ; Zhao, Dun ; Zeng, Zhikui ; Huang, Feng ; Jiang, Ziwei ; Xiong, Hao ; Guan, Tianan ; Fang, Bin ; Li, Yue</creatorcontrib><description>Rhizoma drynariae total flavonoids (RDTF) are used to treat fractures. CD31hiEmcnhi vessels induced by PDGF-BB secreted by osteoclast precursors, together with osteoblasts and osteoclasts, constitute the ternary regulatory mechanism of bone tissue reconstruction.
This study aimed to determine whether RDTF can promote bone tissue remodeling and induce membrane growth in the rat Masquelet model and to explore its molecular mechanism based on the ternary regulation theory.
Thirty-six SD rats were randomized to three groups: blank, induced membrane, and RDTF treatment (n = 12/group). The gross morphological characteristics of the new bone tissue were observed after 6 weeks. Sixty SD rats were also randomized to five groups: blank, induction membrane, low-dose RDTF, medium-dose RDTF, and high-dose RDTF (n = 12/group). After 4 weeks, immunohistochemistry and western blot were used to detect the expression of membrane tissue-related proteins. The mRNA expression of key factors of ternary regulation was analyzed by qRT-PCR.
RDTF positively affected angiogenesis and bone tissue reconstruction in the bone defect area. RDTF could upregulate the expression of key factors (PDGF-BB, CD31, and endomucin), VEGF, and HMGB1 mRNA and proteins in the ternary regulation pathway.
Although the expected CD31hiEmcnhi vessels in the induction membrane were not observed, this study confirmed that RDTF could promote the secretion of angiogenic factors in the induced membrane. The specific mechanisms still need to be further studied.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0278688</identifier><identifier>PMID: 36473008</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Angiogenesis ; Animals ; Becaplermin ; Bioflavonoids ; Biology and Life Sciences ; Bone growth ; Bone regeneration ; Bone Remodeling ; Bones ; Cement ; Defects ; Flavones ; Flavonoids ; Flavonoids - pharmacology ; Fracture fixation ; Fractures ; Gene expression ; Glial stem cells ; Health aspects ; HMGB1 protein ; Immunohistochemistry ; Infections ; Laboratory animals ; Medicine and Health Sciences ; Membranes ; Methods ; Osteoclasts ; Osteoprogenitor cells ; Physical characteristics ; Physical Sciences ; Platelet-derived growth factor ; Platelet-derived growth factor BB ; Polypodiaceae ; Proteins ; Rats ; Rats, Sprague-Dawley ; Reconstruction ; Regulatory mechanisms (biology) ; Research and Analysis Methods ; Skin & tissue grafts ; Surgery ; Tissues ; Vascular endothelial growth factor</subject><ispartof>PloS one, 2022-12, Vol.17 (12), p.e0278688-e0278688</ispartof><rights>Copyright: © 2022 Li et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.</rights><rights>COPYRIGHT 2022 Public Library of Science</rights><rights>2022 Li et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2022 Li et al 2022 Li et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c641t-32f3ee91fd9928235d60f30d94d0e6da54c59639846e72d2c72dd9e55a11f6e43</cites><orcidid>0000-0001-8360-0834</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC9725127/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC9725127/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,2096,2915,23845,27901,27902,53766,53768,79342,79343</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/36473008$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Li, Ding</creatorcontrib><creatorcontrib>Zhao, Dun</creatorcontrib><creatorcontrib>Zeng, Zhikui</creatorcontrib><creatorcontrib>Huang, Feng</creatorcontrib><creatorcontrib>Jiang, Ziwei</creatorcontrib><creatorcontrib>Xiong, Hao</creatorcontrib><creatorcontrib>Guan, Tianan</creatorcontrib><creatorcontrib>Fang, Bin</creatorcontrib><creatorcontrib>Li, Yue</creatorcontrib><title>Ternary regulation mechanism of Rhizoma drynariae total flavonoids on induced membrane formation and bone remodeling in Masquelet technique</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Rhizoma drynariae total flavonoids (RDTF) are used to treat fractures. CD31hiEmcnhi vessels induced by PDGF-BB secreted by osteoclast precursors, together with osteoblasts and osteoclasts, constitute the ternary regulatory mechanism of bone tissue reconstruction.
This study aimed to determine whether RDTF can promote bone tissue remodeling and induce membrane growth in the rat Masquelet model and to explore its molecular mechanism based on the ternary regulation theory.
Thirty-six SD rats were randomized to three groups: blank, induced membrane, and RDTF treatment (n = 12/group). The gross morphological characteristics of the new bone tissue were observed after 6 weeks. Sixty SD rats were also randomized to five groups: blank, induction membrane, low-dose RDTF, medium-dose RDTF, and high-dose RDTF (n = 12/group). After 4 weeks, immunohistochemistry and western blot were used to detect the expression of membrane tissue-related proteins. The mRNA expression of key factors of ternary regulation was analyzed by qRT-PCR.
RDTF positively affected angiogenesis and bone tissue reconstruction in the bone defect area. RDTF could upregulate the expression of key factors (PDGF-BB, CD31, and endomucin), VEGF, and HMGB1 mRNA and proteins in the ternary regulation pathway.
Although the expected CD31hiEmcnhi vessels in the induction membrane were not observed, this study confirmed that RDTF could promote the secretion of angiogenic factors in the induced membrane. The specific mechanisms still need to be further studied.</description><subject>Angiogenesis</subject><subject>Animals</subject><subject>Becaplermin</subject><subject>Bioflavonoids</subject><subject>Biology and Life Sciences</subject><subject>Bone growth</subject><subject>Bone regeneration</subject><subject>Bone Remodeling</subject><subject>Bones</subject><subject>Cement</subject><subject>Defects</subject><subject>Flavones</subject><subject>Flavonoids</subject><subject>Flavonoids - pharmacology</subject><subject>Fracture fixation</subject><subject>Fractures</subject><subject>Gene expression</subject><subject>Glial stem cells</subject><subject>Health aspects</subject><subject>HMGB1 protein</subject><subject>Immunohistochemistry</subject><subject>Infections</subject><subject>Laboratory animals</subject><subject>Medicine and Health Sciences</subject><subject>Membranes</subject><subject>Methods</subject><subject>Osteoclasts</subject><subject>Osteoprogenitor cells</subject><subject>Physical characteristics</subject><subject>Physical Sciences</subject><subject>Platelet-derived growth factor</subject><subject>Platelet-derived growth factor BB</subject><subject>Polypodiaceae</subject><subject>Proteins</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Reconstruction</subject><subject>Regulatory mechanisms (biology)</subject><subject>Research and Analysis Methods</subject><subject>Skin & tissue grafts</subject><subject>Surgery</subject><subject>Tissues</subject><subject>Vascular endothelial growth factor</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><sourceid>DOA</sourceid><recordid>eNqNk11rFDEUhgdRbK3-A9EBQfRi13zMJDM3Qil-FCqFWr0N2eTMbpZMsiYzxfoX_NOedaelK72QgfnIPO97kjc5RfGckjnlkr5bxzEF7eebGGBOmGxE0zwoDmnL2Uwwwh_eeT8onuS8JqTmjRCPiwMuKskJaQ6L35eALum6TLAcvR5cDGUPZqWDy30Zu_Ji5X7FXpc2XSPnNJRDHLQvO6-vYojO5hIlLtjRgEVpv0g6QNnF1O_cdLDlAueIFfpowbuwRLz8ovOPETwM5YDlgsOPp8WjTvsMz6bnUfHt44fLk8-zs_NPpyfHZzMjKjrMOOs4QEs727asYby2gnSc2LayBITVdWXqVvC2qQRIZpnBm22hrjWlnYCKHxUvd74bH7OacsyKyUqyinNJkDjdETbqtdok12NEKmqn_g7EtFQ6Dc54UKxmnFdgGslM1em2WUiQshbUoplcAHq9n6qNix6sgTAk7fdM9_8Et1LLeKVayWrKJBq8mQxSxJTyoHqXDXiPOcdxmrcUlDJEX_2D3r-6iVpqXIALXcS6ZmuqjiVrScvrZlt2fg-Fl4XeGdzQzuH4nuDtngCZAX4OSz3mrE6_Xvw_e_59n319h12B9sMqRz9uT1feB6sdaFLMOUF3GzIlatszN2mobc-oqWdQ9uLuBt2KbpqE_wHtTxO7</recordid><startdate>20221206</startdate><enddate>20221206</enddate><creator>Li, Ding</creator><creator>Zhao, Dun</creator><creator>Zeng, Zhikui</creator><creator>Huang, Feng</creator><creator>Jiang, Ziwei</creator><creator>Xiong, Hao</creator><creator>Guan, Tianan</creator><creator>Fang, Bin</creator><creator>Li, Yue</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0001-8360-0834</orcidid></search><sort><creationdate>20221206</creationdate><title>Ternary regulation mechanism of Rhizoma drynariae total flavonoids on induced membrane formation and bone remodeling in Masquelet technique</title><author>Li, Ding ; Zhao, Dun ; Zeng, Zhikui ; Huang, Feng ; Jiang, Ziwei ; Xiong, Hao ; Guan, Tianan ; Fang, Bin ; Li, Yue</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c641t-32f3ee91fd9928235d60f30d94d0e6da54c59639846e72d2c72dd9e55a11f6e43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Angiogenesis</topic><topic>Animals</topic><topic>Becaplermin</topic><topic>Bioflavonoids</topic><topic>Biology and Life Sciences</topic><topic>Bone growth</topic><topic>Bone regeneration</topic><topic>Bone Remodeling</topic><topic>Bones</topic><topic>Cement</topic><topic>Defects</topic><topic>Flavones</topic><topic>Flavonoids</topic><topic>Flavonoids - pharmacology</topic><topic>Fracture fixation</topic><topic>Fractures</topic><topic>Gene expression</topic><topic>Glial stem cells</topic><topic>Health aspects</topic><topic>HMGB1 protein</topic><topic>Immunohistochemistry</topic><topic>Infections</topic><topic>Laboratory animals</topic><topic>Medicine and Health Sciences</topic><topic>Membranes</topic><topic>Methods</topic><topic>Osteoclasts</topic><topic>Osteoprogenitor cells</topic><topic>Physical characteristics</topic><topic>Physical Sciences</topic><topic>Platelet-derived growth factor</topic><topic>Platelet-derived growth factor BB</topic><topic>Polypodiaceae</topic><topic>Proteins</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Reconstruction</topic><topic>Regulatory mechanisms (biology)</topic><topic>Research and Analysis Methods</topic><topic>Skin & tissue grafts</topic><topic>Surgery</topic><topic>Tissues</topic><topic>Vascular endothelial growth factor</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Li, Ding</creatorcontrib><creatorcontrib>Zhao, Dun</creatorcontrib><creatorcontrib>Zeng, Zhikui</creatorcontrib><creatorcontrib>Huang, Feng</creatorcontrib><creatorcontrib>Jiang, Ziwei</creatorcontrib><creatorcontrib>Xiong, Hao</creatorcontrib><creatorcontrib>Guan, Tianan</creatorcontrib><creatorcontrib>Fang, Bin</creatorcontrib><creatorcontrib>Li, Yue</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: Opposing Viewpoints</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Nursing & Allied Health Database</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Meteorological & Geoastrophysical Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Meteorological & Geoastrophysical Abstracts - Academic</collection><collection>ProQuest Engineering Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Engineering Database</collection><collection>Nursing & Allied Health Premium</collection><collection>Advanced Technologies & Aerospace Database</collection><collection>ProQuest Advanced Technologies & Aerospace Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>Materials Science Collection</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Engineering Collection</collection><collection>Environmental Science Collection</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Li, Ding</au><au>Zhao, Dun</au><au>Zeng, Zhikui</au><au>Huang, Feng</au><au>Jiang, Ziwei</au><au>Xiong, Hao</au><au>Guan, Tianan</au><au>Fang, Bin</au><au>Li, Yue</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Ternary regulation mechanism of Rhizoma drynariae total flavonoids on induced membrane formation and bone remodeling in Masquelet technique</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2022-12-06</date><risdate>2022</risdate><volume>17</volume><issue>12</issue><spage>e0278688</spage><epage>e0278688</epage><pages>e0278688-e0278688</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Rhizoma drynariae total flavonoids (RDTF) are used to treat fractures. CD31hiEmcnhi vessels induced by PDGF-BB secreted by osteoclast precursors, together with osteoblasts and osteoclasts, constitute the ternary regulatory mechanism of bone tissue reconstruction.
This study aimed to determine whether RDTF can promote bone tissue remodeling and induce membrane growth in the rat Masquelet model and to explore its molecular mechanism based on the ternary regulation theory.
Thirty-six SD rats were randomized to three groups: blank, induced membrane, and RDTF treatment (n = 12/group). The gross morphological characteristics of the new bone tissue were observed after 6 weeks. Sixty SD rats were also randomized to five groups: blank, induction membrane, low-dose RDTF, medium-dose RDTF, and high-dose RDTF (n = 12/group). After 4 weeks, immunohistochemistry and western blot were used to detect the expression of membrane tissue-related proteins. The mRNA expression of key factors of ternary regulation was analyzed by qRT-PCR.
RDTF positively affected angiogenesis and bone tissue reconstruction in the bone defect area. RDTF could upregulate the expression of key factors (PDGF-BB, CD31, and endomucin), VEGF, and HMGB1 mRNA and proteins in the ternary regulation pathway.
Although the expected CD31hiEmcnhi vessels in the induction membrane were not observed, this study confirmed that RDTF could promote the secretion of angiogenic factors in the induced membrane. The specific mechanisms still need to be further studied.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>36473008</pmid><doi>10.1371/journal.pone.0278688</doi><tpages>e0278688</tpages><orcidid>https://orcid.org/0000-0001-8360-0834</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2022-12, Vol.17 (12), p.e0278688-e0278688 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_2747243370 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry; Public Library of Science (PLoS) |
| subjects | Angiogenesis Animals Becaplermin Bioflavonoids Biology and Life Sciences Bone growth Bone regeneration Bone Remodeling Bones Cement Defects Flavones Flavonoids Flavonoids - pharmacology Fracture fixation Fractures Gene expression Glial stem cells Health aspects HMGB1 protein Immunohistochemistry Infections Laboratory animals Medicine and Health Sciences Membranes Methods Osteoclasts Osteoprogenitor cells Physical characteristics Physical Sciences Platelet-derived growth factor Platelet-derived growth factor BB Polypodiaceae Proteins Rats Rats, Sprague-Dawley Reconstruction Regulatory mechanisms (biology) Research and Analysis Methods Skin & tissue grafts Surgery Tissues Vascular endothelial growth factor |
| title | Ternary regulation mechanism of Rhizoma drynariae total flavonoids on induced membrane formation and bone remodeling in Masquelet technique |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-08T11%3A35%3A45IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Ternary%20regulation%20mechanism%20of%20Rhizoma%20drynariae%20total%20flavonoids%20on%20induced%20membrane%20formation%20and%20bone%20remodeling%20in%20Masquelet%20technique&rft.jtitle=PloS%20one&rft.au=Li,%20Ding&rft.date=2022-12-06&rft.volume=17&rft.issue=12&rft.spage=e0278688&rft.epage=e0278688&rft.pages=e0278688-e0278688&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0278688&rft_dat=%3Cgale_plos_%3EA729093587%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2747243370&rft_id=info:pmid/36473008&rft_galeid=A729093587&rft_doaj_id=oai_doaj_org_article_252334ec872c4fa98b7e77561d7247be&rfr_iscdi=true |