Characterization of hepatitis B viral forms from patient plasma using velocity gradient: Evidence for an excess of capsids in fractions enriched in Dane particles
Hepatitis B virus (HBV) morphogenesis is characterized by a large over-production of subviral particles and recently described new forms in parallel of complete viral particles (VP). This study was designed to depict circulating viral forms in HBV infected patient plasmas, using velocity gradients a...
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| creator | Pronier, Charlotte Bomo, Jérémy Besombes, Juliette Genet, Valentine Laperche, Syria Gripon, Philippe Thibault, Vincent |
| description | Hepatitis B virus (HBV) morphogenesis is characterized by a large over-production of subviral particles and recently described new forms in parallel of complete viral particles (VP). This study was designed to depict circulating viral forms in HBV infected patient plasmas, using velocity gradients and most sensitive viral markers. Plasmas from chronic hepatitis B (CHB) patients, HBeAg positive or negative, genotype D or E, were fractionated on velocity and equilibrium gradients with or without detergent treatment. Antigenic and molecular markers were measured in plasma and in each collected fraction. Fast Nycodenz velocity gradients revealed good reproducibility and provided additional information to standard equilibrium sucrose gradients. HBV-RNAs circulated as enveloped particles in all plasmas, except one, and at lesser concentrations than VP. Calculations based on standardized measurements and relative virion and subviral particle molecular stoichiometry allowed to refine the experimental approach. For the HBeAg-positive plasma, VP were accompanied by an overproduction of enveloped capsids, either containing HBs, likely corresponding to empty virions, or for the main part, devoid of this viral envelope protein. Similarly, in the HBeAg-negative sample, HBs enveloped capsids, likely corresponding to empty virions, were detected and the presence of enveloped capsids devoid of HBs protein was suspected but not clearly evidenced due to the presence of contaminating high-density subviral particles. While HBeAg largely influences HBcrAg measurement and accounts for two-thirds of HBcrAg reactivity in HBeAg-positive patients, it remains a 10 times more sensitive marker than HBsAg to characterize VP containing fractions. Using Nycodenz velocity gradients and standardized biomarkers, our study proposes a detailed characterization of circulating viral forms in chronically HBV infected patients. We provide evidence for an excess of capsids in fractions enriched in Dane particles, likely due to the presence of empty virions but also by capsids enveloped by an HBs free lipid layer. Identification of this new circulating viral particle sets the basis for studies around the potential role of these entities in hepatitis B pathogeny and their physiological regulation. |
| doi_str_mv | 10.1371/journal.pone.0272474 |
| format | Article |
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This study was designed to depict circulating viral forms in HBV infected patient plasmas, using velocity gradients and most sensitive viral markers. Plasmas from chronic hepatitis B (CHB) patients, HBeAg positive or negative, genotype D or E, were fractionated on velocity and equilibrium gradients with or without detergent treatment. Antigenic and molecular markers were measured in plasma and in each collected fraction. Fast Nycodenz velocity gradients revealed good reproducibility and provided additional information to standard equilibrium sucrose gradients. HBV-RNAs circulated as enveloped particles in all plasmas, except one, and at lesser concentrations than VP. Calculations based on standardized measurements and relative virion and subviral particle molecular stoichiometry allowed to refine the experimental approach. For the HBeAg-positive plasma, VP were accompanied by an overproduction of enveloped capsids, either containing HBs, likely corresponding to empty virions, or for the main part, devoid of this viral envelope protein. Similarly, in the HBeAg-negative sample, HBs enveloped capsids, likely corresponding to empty virions, were detected and the presence of enveloped capsids devoid of HBs protein was suspected but not clearly evidenced due to the presence of contaminating high-density subviral particles. While HBeAg largely influences HBcrAg measurement and accounts for two-thirds of HBcrAg reactivity in HBeAg-positive patients, it remains a 10 times more sensitive marker than HBsAg to characterize VP containing fractions. Using Nycodenz velocity gradients and standardized biomarkers, our study proposes a detailed characterization of circulating viral forms in chronically HBV infected patients. We provide evidence for an excess of capsids in fractions enriched in Dane particles, likely due to the presence of empty virions but also by capsids enveloped by an HBs free lipid layer. Identification of this new circulating viral particle sets the basis for studies around the potential role of these entities in hepatitis B pathogeny and their physiological regulation.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0272474</identifier><identifier>PMID: 36383523</identifier><language>eng</language><publisher>San Francisco: Public Library of Science</publisher><subject>Antigens ; Biology and Life Sciences ; Biomarkers ; Capsids ; Dane particles ; Earth Sciences ; Ecology and Environmental Sciences ; Env protein ; Equilibrium ; Genomes ; Genotype & phenotype ; Genotypes ; Hepatitis ; Hepatitis B ; Hepatitis B e antigen ; Hepatitis B surface antigen ; Infections ; Life Sciences ; Lipids ; Liver cancer ; Medicine and Health Sciences ; Morphogenesis ; Patients ; Physical Sciences ; Plasma ; Plasmas (physics) ; Proteins ; Research and analysis methods ; Stoichiometry ; Sucrose ; Velocity ; Velocity gradient ; Viral envelope proteins ; Virions ; Viruses</subject><ispartof>PloS one, 2022-11, Vol.17 (11), p.e0272474-e0272474</ispartof><rights>2022 Pronier et al. 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This study was designed to depict circulating viral forms in HBV infected patient plasmas, using velocity gradients and most sensitive viral markers. Plasmas from chronic hepatitis B (CHB) patients, HBeAg positive or negative, genotype D or E, were fractionated on velocity and equilibrium gradients with or without detergent treatment. Antigenic and molecular markers were measured in plasma and in each collected fraction. Fast Nycodenz velocity gradients revealed good reproducibility and provided additional information to standard equilibrium sucrose gradients. HBV-RNAs circulated as enveloped particles in all plasmas, except one, and at lesser concentrations than VP. Calculations based on standardized measurements and relative virion and subviral particle molecular stoichiometry allowed to refine the experimental approach. 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We provide evidence for an excess of capsids in fractions enriched in Dane particles, likely due to the presence of empty virions but also by capsids enveloped by an HBs free lipid layer. Identification of this new circulating viral particle sets the basis for studies around the potential role of these entities in hepatitis B pathogeny and their physiological regulation.</description><subject>Antigens</subject><subject>Biology and Life Sciences</subject><subject>Biomarkers</subject><subject>Capsids</subject><subject>Dane particles</subject><subject>Earth Sciences</subject><subject>Ecology and Environmental Sciences</subject><subject>Env protein</subject><subject>Equilibrium</subject><subject>Genomes</subject><subject>Genotype & phenotype</subject><subject>Genotypes</subject><subject>Hepatitis</subject><subject>Hepatitis B</subject><subject>Hepatitis B e antigen</subject><subject>Hepatitis B surface antigen</subject><subject>Infections</subject><subject>Life Sciences</subject><subject>Lipids</subject><subject>Liver cancer</subject><subject>Medicine and Health Sciences</subject><subject>Morphogenesis</subject><subject>Patients</subject><subject>Physical 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of hepatitis B viral forms from patient plasma using velocity gradient: Evidence for an excess of capsids in fractions enriched in Dane particles</title><author>Pronier, Charlotte ; Bomo, Jérémy ; Besombes, Juliette ; Genet, Valentine ; Laperche, Syria ; Gripon, Philippe ; Thibault, Vincent</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c471t-2061fa6e7a296d562c2ecdbe823772c2b4ebe6cae10c0554bf22fff9fb2ebdb3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Antigens</topic><topic>Biology and Life Sciences</topic><topic>Biomarkers</topic><topic>Capsids</topic><topic>Dane particles</topic><topic>Earth Sciences</topic><topic>Ecology and Environmental Sciences</topic><topic>Env protein</topic><topic>Equilibrium</topic><topic>Genomes</topic><topic>Genotype & phenotype</topic><topic>Genotypes</topic><topic>Hepatitis</topic><topic>Hepatitis B</topic><topic>Hepatitis B e 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one</jtitle><date>2022-11-01</date><risdate>2022</risdate><volume>17</volume><issue>11</issue><spage>e0272474</spage><epage>e0272474</epage><pages>e0272474-e0272474</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Hepatitis B virus (HBV) morphogenesis is characterized by a large over-production of subviral particles and recently described new forms in parallel of complete viral particles (VP). This study was designed to depict circulating viral forms in HBV infected patient plasmas, using velocity gradients and most sensitive viral markers. Plasmas from chronic hepatitis B (CHB) patients, HBeAg positive or negative, genotype D or E, were fractionated on velocity and equilibrium gradients with or without detergent treatment. Antigenic and molecular markers were measured in plasma and in each collected fraction. Fast Nycodenz velocity gradients revealed good reproducibility and provided additional information to standard equilibrium sucrose gradients. HBV-RNAs circulated as enveloped particles in all plasmas, except one, and at lesser concentrations than VP. Calculations based on standardized measurements and relative virion and subviral particle molecular stoichiometry allowed to refine the experimental approach. For the HBeAg-positive plasma, VP were accompanied by an overproduction of enveloped capsids, either containing HBs, likely corresponding to empty virions, or for the main part, devoid of this viral envelope protein. Similarly, in the HBeAg-negative sample, HBs enveloped capsids, likely corresponding to empty virions, were detected and the presence of enveloped capsids devoid of HBs protein was suspected but not clearly evidenced due to the presence of contaminating high-density subviral particles. While HBeAg largely influences HBcrAg measurement and accounts for two-thirds of HBcrAg reactivity in HBeAg-positive patients, it remains a 10 times more sensitive marker than HBsAg to characterize VP containing fractions. Using Nycodenz velocity gradients and standardized biomarkers, our study proposes a detailed characterization of circulating viral forms in chronically HBV infected patients. We provide evidence for an excess of capsids in fractions enriched in Dane particles, likely due to the presence of empty virions but also by capsids enveloped by an HBs free lipid layer. Identification of this new circulating viral particle sets the basis for studies around the potential role of these entities in hepatitis B pathogeny and their physiological regulation.</abstract><cop>San Francisco</cop><pub>Public Library of Science</pub><pmid>36383523</pmid><doi>10.1371/journal.pone.0272474</doi><orcidid>https://orcid.org/0000-0001-6517-2901</orcidid><orcidid>https://orcid.org/0000-0002-3065-449X</orcidid><orcidid>https://orcid.org/0000-0002-6497-0108</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Antigens Biology and Life Sciences Biomarkers Capsids Dane particles Earth Sciences Ecology and Environmental Sciences Env protein Equilibrium Genomes Genotype & phenotype Genotypes Hepatitis Hepatitis B Hepatitis B e antigen Hepatitis B surface antigen Infections Life Sciences Lipids Liver cancer Medicine and Health Sciences Morphogenesis Patients Physical Sciences Plasma Plasmas (physics) Proteins Research and analysis methods Stoichiometry Sucrose Velocity Velocity gradient Viral envelope proteins Virions Viruses |
| title | Characterization of hepatitis B viral forms from patient plasma using velocity gradient: Evidence for an excess of capsids in fractions enriched in Dane particles |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-30T16%3A23%3A58IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Characterization%20of%20hepatitis%20B%20viral%20forms%20from%20patient%20plasma%20using%20velocity%20gradient:%20Evidence%20for%20an%20excess%20of%20capsids%20in%20fractions%20enriched%20in%20Dane%20particles&rft.jtitle=PloS%20one&rft.au=Pronier,%20Charlotte&rft.date=2022-11-01&rft.volume=17&rft.issue=11&rft.spage=e0272474&rft.epage=e0272474&rft.pages=e0272474-e0272474&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0272474&rft_dat=%3Cproquest_plos_%3E2737093772%3C/proquest_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2737093772&rft_id=info:pmid/36383523&rfr_iscdi=true |