Progression of microstructural deterioration in load-bearing immobilization osteopenia
Immobilization osteopenia is a major healthcare problem in clinical and social medicine. However, the mechanisms underlying this bone pathology caused by immobilization under load-bearing conditions are not yet fully understood. This study aimed to evaluate sequential changes to the three-dimensiona...
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| creator | Koseki, Hironobu Osaki, Makoto Honda, Yuichiro Sunagawa, Shinya Imai, Chieko Shida, Takayuki Matsumura, Umi Sakamoto, Junya Tomonaga, Iku Yokoo, Seiichi Mizukami, Satoshi Okita, Minoru |
| description | Immobilization osteopenia is a major healthcare problem in clinical and social medicine. However, the mechanisms underlying this bone pathology caused by immobilization under load-bearing conditions are not yet fully understood. This study aimed to evaluate sequential changes to the three-dimensional microstructure of bone in load-bearing immobilization osteopenia using a fixed-limb rat model.
Eight-week-old specific-pathogen-free male Wistar rats were divided into an immobilized group and a control group (n = 60 each). Hind limbs in the immobilized group were fixed using orthopedic casts with fixation periods of 1, 2, 4, 8, and 12 weeks. Feeding and weight-bearing were freely permitted. Length of the right femur was measured after each fixation period and bone microstructure was analyzed by micro-computed tomography. The architectural parameters of cortical and cancellous bone were analyzed statistically.
Femoral length was significantly shorter in the immobilized group than in the control group after 2 weeks. Total area and marrow area were significantly lower in the immobilized group than in the control group from 1 to 12 weeks. Cortical bone area, cortical thickness, and polar moment of inertia decreased significantly after 2 weeks. Some cancellous bone parameters showed osteoporotic changes at 2 weeks after immobilization and the gap with the control group widened as the fixation period extended (P < 0.05).
The present results indicate that load-bearing immobilization triggers early deterioration of microstructure in both cortical and cancellous bone after 2 weeks. |
| doi_str_mv | 10.1371/journal.pone.0275439 |
| format | Article |
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Eight-week-old specific-pathogen-free male Wistar rats were divided into an immobilized group and a control group (n = 60 each). Hind limbs in the immobilized group were fixed using orthopedic casts with fixation periods of 1, 2, 4, 8, and 12 weeks. Feeding and weight-bearing were freely permitted. Length of the right femur was measured after each fixation period and bone microstructure was analyzed by micro-computed tomography. The architectural parameters of cortical and cancellous bone were analyzed statistically.
Femoral length was significantly shorter in the immobilized group than in the control group after 2 weeks. Total area and marrow area were significantly lower in the immobilized group than in the control group from 1 to 12 weeks. Cortical bone area, cortical thickness, and polar moment of inertia decreased significantly after 2 weeks. Some cancellous bone parameters showed osteoporotic changes at 2 weeks after immobilization and the gap with the control group widened as the fixation period extended (P < 0.05).
The present results indicate that load-bearing immobilization triggers early deterioration of microstructure in both cortical and cancellous bone after 2 weeks.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0275439</identifier><identifier>PMID: 36331919</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Animal models ; Animals ; Biology and Life Sciences ; Bone (cancellous) ; Bone (cortical) ; Bone Density ; Bone Diseases, Metabolic - pathology ; Botulinum toxin ; Cancellous bone ; Care and treatment ; Computed tomography ; Cortical bone ; Development and progression ; Femur ; Fixation ; Fracture fixation ; Fractures ; Immobilization ; Immobilization - adverse effects ; Male ; Medicine and Health Sciences ; Metabolism ; Methods ; Microstructure ; Moments of inertia ; Muscle contraction ; Orthopedics ; Osteopenia ; Osteoporosis ; Parameters ; Patient outcomes ; Physical Sciences ; Rats ; Rats, Wistar ; Rodents ; Social medicine ; Specific pathogen free ; Spinal cord ; Weight-Bearing ; X-Ray Microtomography - adverse effects</subject><ispartof>PloS one, 2022-11, Vol.17 (11), p.e0275439-e0275439</ispartof><rights>Copyright: © 2022 Koseki et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.</rights><rights>COPYRIGHT 2022 Public Library of Science</rights><rights>2022 Koseki et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2022 Koseki et al 2022 Koseki et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c802t-b1a1ea82207ce1f6624c04fca48380dc46bfc5e530ea4cec8e4b1ec4f49874263</citedby><cites>FETCH-LOGICAL-c802t-b1a1ea82207ce1f6624c04fca48380dc46bfc5e530ea4cec8e4b1ec4f49874263</cites><orcidid>0000-0001-5621-2756 ; 0000-0002-9097-2115 ; 0000-0001-5047-2311</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC9635731/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC9635731/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,2102,2928,23866,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/36331919$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Tomaszewska, Ewa</contributor><creatorcontrib>Koseki, Hironobu</creatorcontrib><creatorcontrib>Osaki, Makoto</creatorcontrib><creatorcontrib>Honda, Yuichiro</creatorcontrib><creatorcontrib>Sunagawa, Shinya</creatorcontrib><creatorcontrib>Imai, Chieko</creatorcontrib><creatorcontrib>Shida, Takayuki</creatorcontrib><creatorcontrib>Matsumura, Umi</creatorcontrib><creatorcontrib>Sakamoto, Junya</creatorcontrib><creatorcontrib>Tomonaga, Iku</creatorcontrib><creatorcontrib>Yokoo, Seiichi</creatorcontrib><creatorcontrib>Mizukami, Satoshi</creatorcontrib><creatorcontrib>Okita, Minoru</creatorcontrib><title>Progression of microstructural deterioration in load-bearing immobilization osteopenia</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Immobilization osteopenia is a major healthcare problem in clinical and social medicine. However, the mechanisms underlying this bone pathology caused by immobilization under load-bearing conditions are not yet fully understood. This study aimed to evaluate sequential changes to the three-dimensional microstructure of bone in load-bearing immobilization osteopenia using a fixed-limb rat model.
Eight-week-old specific-pathogen-free male Wistar rats were divided into an immobilized group and a control group (n = 60 each). Hind limbs in the immobilized group were fixed using orthopedic casts with fixation periods of 1, 2, 4, 8, and 12 weeks. Feeding and weight-bearing were freely permitted. Length of the right femur was measured after each fixation period and bone microstructure was analyzed by micro-computed tomography. The architectural parameters of cortical and cancellous bone were analyzed statistically.
Femoral length was significantly shorter in the immobilized group than in the control group after 2 weeks. Total area and marrow area were significantly lower in the immobilized group than in the control group from 1 to 12 weeks. Cortical bone area, cortical thickness, and polar moment of inertia decreased significantly after 2 weeks. Some cancellous bone parameters showed osteoporotic changes at 2 weeks after immobilization and the gap with the control group widened as the fixation period extended (P < 0.05).
The present results indicate that load-bearing immobilization triggers early deterioration of microstructure in both cortical and cancellous bone after 2 weeks.</description><subject>Animal models</subject><subject>Animals</subject><subject>Biology and Life Sciences</subject><subject>Bone (cancellous)</subject><subject>Bone (cortical)</subject><subject>Bone Density</subject><subject>Bone Diseases, Metabolic - pathology</subject><subject>Botulinum toxin</subject><subject>Cancellous bone</subject><subject>Care and treatment</subject><subject>Computed tomography</subject><subject>Cortical bone</subject><subject>Development and progression</subject><subject>Femur</subject><subject>Fixation</subject><subject>Fracture fixation</subject><subject>Fractures</subject><subject>Immobilization</subject><subject>Immobilization - adverse effects</subject><subject>Male</subject><subject>Medicine and Health Sciences</subject><subject>Metabolism</subject><subject>Methods</subject><subject>Microstructure</subject><subject>Moments of inertia</subject><subject>Muscle contraction</subject><subject>Orthopedics</subject><subject>Osteopenia</subject><subject>Osteoporosis</subject><subject>Parameters</subject><subject>Patient outcomes</subject><subject>Physical Sciences</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Rodents</subject><subject>Social medicine</subject><subject>Specific pathogen free</subject><subject>Spinal cord</subject><subject>Weight-Bearing</subject><subject>X-Ray Microtomography - 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pathology</topic><topic>Botulinum toxin</topic><topic>Cancellous bone</topic><topic>Care and treatment</topic><topic>Computed tomography</topic><topic>Cortical bone</topic><topic>Development and progression</topic><topic>Femur</topic><topic>Fixation</topic><topic>Fracture fixation</topic><topic>Fractures</topic><topic>Immobilization</topic><topic>Immobilization - adverse effects</topic><topic>Male</topic><topic>Medicine and Health Sciences</topic><topic>Metabolism</topic><topic>Methods</topic><topic>Microstructure</topic><topic>Moments of inertia</topic><topic>Muscle contraction</topic><topic>Orthopedics</topic><topic>Osteopenia</topic><topic>Osteoporosis</topic><topic>Parameters</topic><topic>Patient outcomes</topic><topic>Physical Sciences</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Rodents</topic><topic>Social medicine</topic><topic>Specific pathogen free</topic><topic>Spinal cord</topic><topic>Weight-Bearing</topic><topic>X-Ray Microtomography - adverse effects</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Koseki, Hironobu</creatorcontrib><creatorcontrib>Osaki, Makoto</creatorcontrib><creatorcontrib>Honda, Yuichiro</creatorcontrib><creatorcontrib>Sunagawa, Shinya</creatorcontrib><creatorcontrib>Imai, Chieko</creatorcontrib><creatorcontrib>Shida, Takayuki</creatorcontrib><creatorcontrib>Matsumura, Umi</creatorcontrib><creatorcontrib>Sakamoto, Junya</creatorcontrib><creatorcontrib>Tomonaga, Iku</creatorcontrib><creatorcontrib>Yokoo, Seiichi</creatorcontrib><creatorcontrib>Mizukami, Satoshi</creatorcontrib><creatorcontrib>Okita, Minoru</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: Opposing Viewpoints</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Nursing & Allied Health Database</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Meteorological & Geoastrophysical Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Meteorological & Geoastrophysical Abstracts - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Koseki, Hironobu</au><au>Osaki, Makoto</au><au>Honda, Yuichiro</au><au>Sunagawa, Shinya</au><au>Imai, Chieko</au><au>Shida, Takayuki</au><au>Matsumura, Umi</au><au>Sakamoto, Junya</au><au>Tomonaga, Iku</au><au>Yokoo, Seiichi</au><au>Mizukami, Satoshi</au><au>Okita, Minoru</au><au>Tomaszewska, Ewa</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Progression of microstructural deterioration in load-bearing immobilization osteopenia</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2022-11-04</date><risdate>2022</risdate><volume>17</volume><issue>11</issue><spage>e0275439</spage><epage>e0275439</epage><pages>e0275439-e0275439</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Immobilization osteopenia is a major healthcare problem in clinical and social medicine. However, the mechanisms underlying this bone pathology caused by immobilization under load-bearing conditions are not yet fully understood. This study aimed to evaluate sequential changes to the three-dimensional microstructure of bone in load-bearing immobilization osteopenia using a fixed-limb rat model.
Eight-week-old specific-pathogen-free male Wistar rats were divided into an immobilized group and a control group (n = 60 each). Hind limbs in the immobilized group were fixed using orthopedic casts with fixation periods of 1, 2, 4, 8, and 12 weeks. Feeding and weight-bearing were freely permitted. Length of the right femur was measured after each fixation period and bone microstructure was analyzed by micro-computed tomography. The architectural parameters of cortical and cancellous bone were analyzed statistically.
Femoral length was significantly shorter in the immobilized group than in the control group after 2 weeks. Total area and marrow area were significantly lower in the immobilized group than in the control group from 1 to 12 weeks. Cortical bone area, cortical thickness, and polar moment of inertia decreased significantly after 2 weeks. Some cancellous bone parameters showed osteoporotic changes at 2 weeks after immobilization and the gap with the control group widened as the fixation period extended (P < 0.05).
The present results indicate that load-bearing immobilization triggers early deterioration of microstructure in both cortical and cancellous bone after 2 weeks.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>36331919</pmid><doi>10.1371/journal.pone.0275439</doi><tpages>e0275439</tpages><orcidid>https://orcid.org/0000-0001-5621-2756</orcidid><orcidid>https://orcid.org/0000-0002-9097-2115</orcidid><orcidid>https://orcid.org/0000-0001-5047-2311</orcidid><oa>free_for_read</oa></addata></record> |
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| ispartof | PloS one, 2022-11, Vol.17 (11), p.e0275439-e0275439 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_2731999620 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Public Library of Science (PLoS) Journals Open Access; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Animal models Animals Biology and Life Sciences Bone (cancellous) Bone (cortical) Bone Density Bone Diseases, Metabolic - pathology Botulinum toxin Cancellous bone Care and treatment Computed tomography Cortical bone Development and progression Femur Fixation Fracture fixation Fractures Immobilization Immobilization - adverse effects Male Medicine and Health Sciences Metabolism Methods Microstructure Moments of inertia Muscle contraction Orthopedics Osteopenia Osteoporosis Parameters Patient outcomes Physical Sciences Rats Rats, Wistar Rodents Social medicine Specific pathogen free Spinal cord Weight-Bearing X-Ray Microtomography - adverse effects |
| title | Progression of microstructural deterioration in load-bearing immobilization osteopenia |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-21T05%3A15%3A39IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Progression%20of%20microstructural%20deterioration%20in%20load-bearing%20immobilization%20osteopenia&rft.jtitle=PloS%20one&rft.au=Koseki,%20Hironobu&rft.date=2022-11-04&rft.volume=17&rft.issue=11&rft.spage=e0275439&rft.epage=e0275439&rft.pages=e0275439-e0275439&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0275439&rft_dat=%3Cgale_plos_%3EA725074035%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2731999620&rft_id=info:pmid/36331919&rft_galeid=A725074035&rft_doaj_id=oai_doaj_org_article_483653e4982b4e62a502c180b410d39c&rfr_iscdi=true |