Skin microbiota of oxazolone-induced contact hypersensitivity mouse model
Contact allergy is a common skin allergy, which can be studied utilising contact hypersensitivity (CHS) animal model. However, it is not clear, whether CHS is a suitable model to investigate skin microbiota interactions. We characterised the effect of contact dermatitis on the skin microbiota and st...
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| description | Contact allergy is a common skin allergy, which can be studied utilising contact hypersensitivity (CHS) animal model. However, it is not clear, whether CHS is a suitable model to investigate skin microbiota interactions. We characterised the effect of contact dermatitis on the skin microbiota and studied the biological effects of oxazolone (OXA) -induced inflammation on skin thickness, immune cell numbers and changes of the microbiota in CHS mouse model (n = 72) for 28 days. Through 16S rRNA gene sequencing we defined the composition of bacterial communities and associations of bacteria with inflammation. We observed that the vehicle solution of acetone and olive oil induced bacterial community changes on day 1, and OXA-induced changes were observed mainly on day 7. Many of the notably enriched bacteria present in the OXA-challenged positive group represented the genus Faecalibaculum which were most likely derived from the cage environment. Additionally, skin inflammation correlated negatively with Streptococcus, which is considered a native skin bacterium, and positively with Muribacter muris, which is typical in oral environment. Skin inflammation favoured colonisation of cage-derived faecal bacteria, and additionally mouse grooming transferred oral bacteria on the skin. Due to the observed changes, we conclude that CHS model could be used for certain skin microbiome-related research set-ups. However, since vehicle exposure can alter the skin microbiome as such, future studies should include considerations such as careful control sampling and statistical tests to account for potential confounding factors. |
| doi_str_mv | 10.1371/journal.pone.0276071 |
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However, it is not clear, whether CHS is a suitable model to investigate skin microbiota interactions. We characterised the effect of contact dermatitis on the skin microbiota and studied the biological effects of oxazolone (OXA) -induced inflammation on skin thickness, immune cell numbers and changes of the microbiota in CHS mouse model (n = 72) for 28 days. Through 16S rRNA gene sequencing we defined the composition of bacterial communities and associations of bacteria with inflammation. We observed that the vehicle solution of acetone and olive oil induced bacterial community changes on day 1, and OXA-induced changes were observed mainly on day 7. Many of the notably enriched bacteria present in the OXA-challenged positive group represented the genus Faecalibaculum which were most likely derived from the cage environment. Additionally, skin inflammation correlated negatively with Streptococcus, which is considered a native skin bacterium, and positively with Muribacter muris, which is typical in oral environment. Skin inflammation favoured colonisation of cage-derived faecal bacteria, and additionally mouse grooming transferred oral bacteria on the skin. Due to the observed changes, we conclude that CHS model could be used for certain skin microbiome-related research set-ups. However, since vehicle exposure can alter the skin microbiome as such, future studies should include considerations such as careful control sampling and statistical tests to account for potential confounding factors.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0276071</identifier><language>eng</language><publisher>San Francisco: Public Library of Science</publisher><subject>Acetone ; Analysis ; Animal models ; Bacteria ; Biological effects ; Biology and Life Sciences ; Biopsy ; Cages ; Care and treatment ; Colonization ; Complications and side effects ; Contact dermatitis ; Diagnosis ; Experiments ; Gene sequencing ; Grooming ; Haptens ; Histology ; Hypersensitivity ; Immune system ; Inflammation ; Laboratory animals ; Medicine and Health Sciences ; Microbiomes ; Microbiota ; Microbiota (Symbiotic organisms) ; Modelling ; Neutrophils ; Olive oil ; Oxazolone ; Research and Analysis Methods ; rRNA 16S ; Skin ; Skin tests ; Statistical analysis ; Statistical tests</subject><ispartof>PloS one, 2022-10, Vol.17 (10), p.e0276071-e0276071</ispartof><rights>COPYRIGHT 2022 Public Library of Science</rights><rights>2022 Mäenpää et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. 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However, it is not clear, whether CHS is a suitable model to investigate skin microbiota interactions. We characterised the effect of contact dermatitis on the skin microbiota and studied the biological effects of oxazolone (OXA) -induced inflammation on skin thickness, immune cell numbers and changes of the microbiota in CHS mouse model (n = 72) for 28 days. Through 16S rRNA gene sequencing we defined the composition of bacterial communities and associations of bacteria with inflammation. We observed that the vehicle solution of acetone and olive oil induced bacterial community changes on day 1, and OXA-induced changes were observed mainly on day 7. Many of the notably enriched bacteria present in the OXA-challenged positive group represented the genus Faecalibaculum which were most likely derived from the cage environment. 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Harri</au><au>Sinkko, Hanna</au><au>Karisola, Piia</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Skin microbiota of oxazolone-induced contact hypersensitivity mouse model</atitle><jtitle>PloS one</jtitle><date>2022-10-20</date><risdate>2022</risdate><volume>17</volume><issue>10</issue><spage>e0276071</spage><epage>e0276071</epage><pages>e0276071-e0276071</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Contact allergy is a common skin allergy, which can be studied utilising contact hypersensitivity (CHS) animal model. However, it is not clear, whether CHS is a suitable model to investigate skin microbiota interactions. We characterised the effect of contact dermatitis on the skin microbiota and studied the biological effects of oxazolone (OXA) -induced inflammation on skin thickness, immune cell numbers and changes of the microbiota in CHS mouse model (n = 72) for 28 days. Through 16S rRNA gene sequencing we defined the composition of bacterial communities and associations of bacteria with inflammation. We observed that the vehicle solution of acetone and olive oil induced bacterial community changes on day 1, and OXA-induced changes were observed mainly on day 7. Many of the notably enriched bacteria present in the OXA-challenged positive group represented the genus Faecalibaculum which were most likely derived from the cage environment. 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| source | Public Library of Science (PLoS) Journals Open Access; Full-Text Journals in Chemistry (Open access); EZB Free E-Journals; DOAJ Directory of Open Access Journals; PubMed Central; SWEPUB Freely available online |
| subjects | Acetone Analysis Animal models Bacteria Biological effects Biology and Life Sciences Biopsy Cages Care and treatment Colonization Complications and side effects Contact dermatitis Diagnosis Experiments Gene sequencing Grooming Haptens Histology Hypersensitivity Immune system Inflammation Laboratory animals Medicine and Health Sciences Microbiomes Microbiota Microbiota (Symbiotic organisms) Modelling Neutrophils Olive oil Oxazolone Research and Analysis Methods rRNA 16S Skin Skin tests Statistical analysis Statistical tests |
| title | Skin microbiota of oxazolone-induced contact hypersensitivity mouse model |
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