Coelenterazine sulfotransferase from Renilla muelleri
The luciferin sulfokinase (coelenterazine sulfotransferase) of Renilla was previously reported to activate the storage form, luciferyl sulfate (coelenterazine sulfate) to luciferin (coelenterazine), the substrate for the luciferase bioluminescence reaction. The gene coding for the coelenterazine sul...
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| creator | Tzertzinis, George Baker, Brenda Benner, Jack Brown, Elizabeth Corrêa, Ivan R Ettwiller, Laurence McClung, Colleen Schildkraut, Ira |
| description | The luciferin sulfokinase (coelenterazine sulfotransferase) of Renilla was previously reported to activate the storage form, luciferyl sulfate (coelenterazine sulfate) to luciferin (coelenterazine), the substrate for the luciferase bioluminescence reaction. The gene coding for the coelenterazine sulfotransferase has not been identified. Here we used a combined proteomic/transcriptomic approach to identify and clone the sulfotransferase cDNA. Multiple isoforms of coelenterazine sulfotransferase were identified from the anthozoan Renilla muelleri by intersecting its transcriptome with the LC-MS/MS derived peptide sequences of coelenterazine sulfotransferase purified from Renilla. Two of the isoforms were expressed in E. coli, purified, and partially characterized. The encoded enzymes display sulfotransferase activity that is comparable to that of the native sulfotransferase isolated from Renilla reniformis that was reported in 1970. The bioluminescent assay for sensitive detection of 3'-phosphoadenosine 5'-phosphate (PAP) using the recombinant sulfotransferase is demonstrated. |
| doi_str_mv | 10.1371/journal.pone.0276315 |
| format | Article |
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The gene coding for the coelenterazine sulfotransferase has not been identified. Here we used a combined proteomic/transcriptomic approach to identify and clone the sulfotransferase cDNA. Multiple isoforms of coelenterazine sulfotransferase were identified from the anthozoan Renilla muelleri by intersecting its transcriptome with the LC-MS/MS derived peptide sequences of coelenterazine sulfotransferase purified from Renilla. Two of the isoforms were expressed in E. coli, purified, and partially characterized. The encoded enzymes display sulfotransferase activity that is comparable to that of the native sulfotransferase isolated from Renilla reniformis that was reported in 1970. The bioluminescent assay for sensitive detection of 3'-phosphoadenosine 5'-phosphate (PAP) using the recombinant sulfotransferase is demonstrated.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0276315</identifier><identifier>PMID: 36251663</identifier><language>eng</language><publisher>San Francisco: Public Library of Science</publisher><subject>Analysis ; Biology and Life Sciences ; Bioluminescence ; Chromatography ; Cloning ; E coli ; Enzymes ; Genes ; Genetic aspects ; Glycerol ; Homogenization ; Isoforms ; Luciferin ; Molecular weight ; Particle size ; Peptides ; Physical Sciences ; Proteins ; Proteomics ; Renilla muelleri ; Research and Analysis Methods ; Substrates ; Sulfates ; Sulfotransferase ; Transcriptomes ; Transcriptomics</subject><ispartof>PloS one, 2022-10, Vol.17 (10), p.e0276315-e0276315</ispartof><rights>COPYRIGHT 2022 Public Library of Science</rights><rights>2022 Tzertzinis et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2022 Tzertzinis et al 2022 Tzertzinis et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c669t-fbc5966606c3816b4cb95d2ef6de953f258c6b6685acd78d80e88c68a0106ad3</citedby><cites>FETCH-LOGICAL-c669t-fbc5966606c3816b4cb95d2ef6de953f258c6b6685acd78d80e88c68a0106ad3</cites><orcidid>0000-0001-8821-8060 ; 0000-0002-0070-6033 ; 0000-0002-3169-6878 ; 0000-0002-5801-1031</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC9576082/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC9576082/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,2102,2928,23866,27924,27925,53791,53793</link.rule.ids></links><search><contributor>Wang, Tianwen</contributor><creatorcontrib>Tzertzinis, George</creatorcontrib><creatorcontrib>Baker, Brenda</creatorcontrib><creatorcontrib>Benner, Jack</creatorcontrib><creatorcontrib>Brown, Elizabeth</creatorcontrib><creatorcontrib>Corrêa, Ivan R</creatorcontrib><creatorcontrib>Ettwiller, Laurence</creatorcontrib><creatorcontrib>McClung, Colleen</creatorcontrib><creatorcontrib>Schildkraut, Ira</creatorcontrib><title>Coelenterazine sulfotransferase from Renilla muelleri</title><title>PloS one</title><description>The luciferin sulfokinase (coelenterazine sulfotransferase) of Renilla was previously reported to activate the storage form, luciferyl sulfate (coelenterazine sulfate) to luciferin (coelenterazine), the substrate for the luciferase bioluminescence reaction. The gene coding for the coelenterazine sulfotransferase has not been identified. Here we used a combined proteomic/transcriptomic approach to identify and clone the sulfotransferase cDNA. Multiple isoforms of coelenterazine sulfotransferase were identified from the anthozoan Renilla muelleri by intersecting its transcriptome with the LC-MS/MS derived peptide sequences of coelenterazine sulfotransferase purified from Renilla. Two of the isoforms were expressed in E. coli, purified, and partially characterized. The encoded enzymes display sulfotransferase activity that is comparable to that of the native sulfotransferase isolated from Renilla reniformis that was reported in 1970. The bioluminescent assay for sensitive detection of 3'-phosphoadenosine 5'-phosphate (PAP) using the recombinant sulfotransferase is demonstrated.</description><subject>Analysis</subject><subject>Biology and Life Sciences</subject><subject>Bioluminescence</subject><subject>Chromatography</subject><subject>Cloning</subject><subject>E coli</subject><subject>Enzymes</subject><subject>Genes</subject><subject>Genetic aspects</subject><subject>Glycerol</subject><subject>Homogenization</subject><subject>Isoforms</subject><subject>Luciferin</subject><subject>Molecular weight</subject><subject>Particle size</subject><subject>Peptides</subject><subject>Physical Sciences</subject><subject>Proteins</subject><subject>Proteomics</subject><subject>Renilla muelleri</subject><subject>Research and Analysis 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Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tzertzinis, George</au><au>Baker, Brenda</au><au>Benner, Jack</au><au>Brown, Elizabeth</au><au>Corrêa, Ivan R</au><au>Ettwiller, Laurence</au><au>McClung, Colleen</au><au>Schildkraut, Ira</au><au>Wang, Tianwen</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Coelenterazine sulfotransferase from Renilla muelleri</atitle><jtitle>PloS one</jtitle><date>2022-10-17</date><risdate>2022</risdate><volume>17</volume><issue>10</issue><spage>e0276315</spage><epage>e0276315</epage><pages>e0276315-e0276315</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>The luciferin sulfokinase (coelenterazine sulfotransferase) of Renilla was previously reported to activate the storage form, luciferyl sulfate (coelenterazine sulfate) to luciferin (coelenterazine), the substrate for the luciferase bioluminescence reaction. The gene coding for the coelenterazine sulfotransferase has not been identified. Here we used a combined proteomic/transcriptomic approach to identify and clone the sulfotransferase cDNA. Multiple isoforms of coelenterazine sulfotransferase were identified from the anthozoan Renilla muelleri by intersecting its transcriptome with the LC-MS/MS derived peptide sequences of coelenterazine sulfotransferase purified from Renilla. Two of the isoforms were expressed in E. coli, purified, and partially characterized. The encoded enzymes display sulfotransferase activity that is comparable to that of the native sulfotransferase isolated from Renilla reniformis that was reported in 1970. The bioluminescent assay for sensitive detection of 3'-phosphoadenosine 5'-phosphate (PAP) using the recombinant sulfotransferase is demonstrated.</abstract><cop>San Francisco</cop><pub>Public Library of Science</pub><pmid>36251663</pmid><doi>10.1371/journal.pone.0276315</doi><tpages>e0276315</tpages><orcidid>https://orcid.org/0000-0001-8821-8060</orcidid><orcidid>https://orcid.org/0000-0002-0070-6033</orcidid><orcidid>https://orcid.org/0000-0002-3169-6878</orcidid><orcidid>https://orcid.org/0000-0002-5801-1031</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Analysis Biology and Life Sciences Bioluminescence Chromatography Cloning E coli Enzymes Genes Genetic aspects Glycerol Homogenization Isoforms Luciferin Molecular weight Particle size Peptides Physical Sciences Proteins Proteomics Renilla muelleri Research and Analysis Methods Substrates Sulfates Sulfotransferase Transcriptomes Transcriptomics |
| title | Coelenterazine sulfotransferase from Renilla muelleri |
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